Antibody libraries

ABSTRACT

The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity.

RELATED APPLICATION

This application is a national phase entry under 35 U.S.C.§371 ofInternational Application No. PCT/US2011/044063, filed Jul. 14, 2011,which claims priority to U.S. provisional application Ser. No.61/365,194, filed on Jul 16, 2010, incorporated herein in their entiretyby this reference.

SEQUENCE LISTING

In accordance with 37 CFR 1.52(e)(5), a Sequence Listing in the form ofa text file (entitled “2009186-0093 ST25”created on Dec. 22, 2015, and2,508,849 bytes in size) is incorporated by reference in its entirety.

BACKGROUND

Antibodies have profound relevance as research tools and in diagnosticand therapeutic applications. However, the identification of usefulantibodies is difficult and once identified, antibodies often requireconsiderable redesign or “humanization” before they are suitable fortherapeutic applications in humans.

Many methods for identifying antibodies involve display of libraries ofantibodies derived by amplification of nucleic acids from B cells ortissues. Some of these methods have utilized synthetic libraries.However, many of these approaches have limitations. For example, mosthuman antibody libraries known in the art contain only the antibodysequence diversity that can be experimentally captured or cloned from abiological source (e.g., B cells). Accordingly, such libraries mayover-represent some sequences, while completely lacking orunder-representing other sequences, particularly those binding humanantigens. Most synthetic libraries known in the art have otherlimitations, such as the occurrence of unnatural (i.e., non-human) aminoacid sequence motifs that have the potential to be immunogenic.

Accordingly, a need exists for diverse antibody libraries that containcandidate antibodies that are non-immunogenic (i.e., are human) and havedesired properties (e.g., the ability to recognize a broad variety ofantigens). However, obtaining such libraries requires balancing thecompeting objectives of generating diverse libraries while stillmaintaining the human character of the sequences within the library. Thecurrent invention provides antibody libraries that have these and otherdesirable features, and methods of making and using such libraries.

SUMMARY

The invention provides, among other things, improvements in the designand production of synthetic libraries that mimic the diversity of thenatural human repertoire of CDRH3, CDRL3, heavy chain, light chain,and/or full-length (intact) antibody sequences. In some embodiments theinvention defines and provides methods of generating theoretical segmentpools of TN1, DH, N2, and H3-JH segments to consider for inclusion in aphysical manifestation of a library (e.g., polynucleotide orpolypeptide) comprising or encoding CDRH3 sequences (e.g., an antibodylibrary). In certain embodiments the invention defines and providesmethods of matching the individual members of these theoretical segmentpools to a reference set of CDRH3 sequences, to determine the frequencyof occurrence (or segment usage weight) of each of the segments in thetheoretical segment pool in the reference set. While any set of CDRH3sequences may be used as a reference set, the invention also defines andprovides methods of generating particular reference sets or subsets ofinterest. For example, among other things, the present inventionprovides methods for filtering an original reference set to obtain aprovided reference set with a preimmune character. Also provided aremethods to define and/or identify segments that occur within the CDRH3sequences in the reference set but not in the theoretical segment pool.Such segments can be added to a theoretical segment pool, for example inorder to be considered for inclusion in a physical library. Although thefrequency of occurrence of a particular segment in a reference set isuseful to select segments for inclusion in a physical library, theinvention also provides a number of physicochemical and biologicalproperties that can be used (alone or together with any other criterionor criteria) to select segments for inclusion in a physical library.

In some embodiments the invention provides libraries that differ fromcertain other libraries known in the art in that they are notsitewise-stochastic in composition or sequence, and are thereforeintrinsically less random than these certain other libraries of the art(see e.g., Example 14 of US Pub. No. 2009/0181855, incorporated byreference in its entirety, for a discussion of information content andrandomness). In some embodiments, degenerate oligonucleotides may beused to further increase the diversity of the members of a library whilefurther improving matching with a reference set of sequences (e.g.,CDRH3, CDRL3, heavy chain, light chain, and/or full-length (intact)antibody sequences).

The invention also provides libraries whose members have sequences thatare related to one another in that they would be selected for inclusionin a physical library by performing the analyses described herein, forexample by generating a CDRH3 reference set as in Example 3; generatingtheoretical segment pools as in Examples 5-7; matching the members of atheoretical segment pool to the reference set as in Examples 4 and 8;and selecting members of the theoretical segment pool for inclusion in aphysical library as in Examples 8-9. Also provided are methods offurther increasing diversity in certain sequences by utilizingdegenerate oligonucleotides as in Examples 12-16.

In some embodiments, the present invention provides polynucleotide andpolypeptide libraries comprising CDRH3, CDRL3, heavy chain, light chain,and/or full-length (intact) antibody sequences, and methods of makingand using such libraries.

In some embodiments, the invention provides libraries comprising,consisting essentially of, or consisting of any of the libraries ortheoretical segment pools described herein.

In some embodiments, the present invention recognizes that by mimickingthe in vivo activity of the enzyme TdT computationally, theoreticalsegment pools can be generated and subsequently matched to largereference datasets of CDR sequences to choose, for inclusion in alibrary, those theoretical segments that best recapitulate the CDRsequences in the reference data sets.

In certain embodiments, the invention provides libraries ofpolynucleotides comprising at least about 10⁴ polynucleotides encodingCDRH3 polypeptides with the structure: [TN1]-[DH]-[N2]-[H3-JH], wherein:TN1 is a polypeptide corresponding to any of the TN1 polypeptides ofTables 9-10 and 18-26, or a polypeptide produced by translation of anyof the TN1 polynucleotides of Tables 25-26; DH is a polypeptidecorresponding to any of the DH polypeptides of Tables 9, 11, 17-25 and28, or a polypeptide produced by translation of any of the DH-encodingpolynucleotides of Tables 16, 25 and 27; N2 is a polypeptidecorresponding to any of the N2 polypeptides of Tables 9, 12, 18-25 and30, or a polypeptide produced by translation of any of the N2-encodingpolynucleotides of Tables 25 and 29; and H3-JH is a polypeptidecorresponding to any of the H3-JH polypeptides of Tables 9, 13, 15,18-25 and 32, or a polypeptide produced by translation of any of theH3-JH-encoding polynucleotides of Tables 14, 25 and 31.

In some embodiments, the invention provides libraries wherein at leastabout 1%, 5%, or 10% of the sequences in the library have the structureprovided above, or that of any of the libraries provided herein.

In certain embodiments, the invention provides libraries comprisingpolynucleotides encoding CDRH3 polypeptides produced by the sets of TN1,DH, N2, and H3-JH polypeptides provided in any one of Tables 23-25.

In some embodiments, the invention provides libraries comprisingpolynucleotides encoding CDRH3 polypeptides produced by the set of TN1polypeptides provided in Table 26, the set of DH polypeptides providedin Table 28, the set of N2 polypeptides provided in Table 30 and the setof H3-JH polypeptides provided in Table 32.

In certain embodiments, the invention provides libraries whose membersshow (or encode polypeptides that show) at least a certain percentidentity with the polypeptides described above, for example, a librarycomprising at least about 10⁴ polynucleotides encoding CDRH3polypeptides with the structure: [TN1]-[DH]-[N2]-[H3-JH], wherein: TN1is a polypeptide at least about 80%, 90%, or 95% identical to any of theTN1 polypeptides of Tables 9-10 and 18-26, or a polypeptide at leastabout 80%, 90%, or 95% identical to a polypeptide produced bytranslation of any of the TN1 polynucleotides of Tables 25-26; DH is apolypeptide at least about 80%, 90%, or 95% identical to any of the DHpolypeptides of Tables 9, 11, 17-25 and 28, or a polypeptide at leastabout 80%, 90%, or 95% identical to a polypeptide produced bytranslation of any of the DH-encoding polynucleotides of Tables 16, 25and 27; N2 is a polypeptide at least about 80%, 90%, or 95% identical toany of the N2 polypeptides of Tables 9, 12, 18-25 and 30, or apolypeptide at least about 80%, 90%, or 95% identical to a polypeptideproduced by translation of any of the N2-encoding polynucleotides ofTables 25 and 29; and H3-JH is a polypeptide at least about 80%, 90%, or95% identical to any of the H3-JH polypeptides of Tables 9, 13, 15,18-25 and 32, or a polypeptide at least about 80%, 90%, or 95% identicalto a polypeptide produced by translation of any of the H3-JH-encodingpolynucleotides of Tables 14, 25 and 31.

In some embodiments, the invention provides libraries comprisingpolynucleotides encoding light chain variable regions, wherein the lightchain variable regions are selected from the group consisting of: (a) aVK1-05 sequence varied at one or more of positions 4, 49, and 46; (b) aVK1-12 sequence varied at one or more of positions 4, 49, 46, and 66;(c) a VK1-33 sequence varied at one or more of positions 4, 49, and 66;(d) a VK1-39 sequence varied at one or more of positions 4, 49, and 46;(e) a VK2-28 sequence varied at one or more of positions 2, 4, 46, and49; (f) a VK3-11 sequence varied at one or more of positions 2, 4, 36,and 49; (g) a VK3-15 sequence varied at one or more of positions 2, 4,48, and 49; (h) a VK3-20 sequence varied at one or more of positions 2,4, 48, and 49; and/or (i) a VK4-1 sequence varied at one or more ofpositions 4, 46, 49, and 66.

In certain embodiments, the invention provides libraries comprisingpolynucleotides encoding light chain variable regions that comprisepolypeptide sequences at least about 80%, 90%, or 95% identical to twoor more of the light chain polypeptide sequences provided in Table 3.

In some embodiments, the invention provides libraries wherein the lightchain variable regions comprise the polypeptide sequences provided inTable 3.

In certain embodiments, the invention provides libraries comprisingpolynucleotides encoding light chain variable regions, wherein the L3-VLpolypeptide sequences of the light chain variable regions are varied attwo or three residues between positions 89 to 94, inclusive, incomparison to an L3-VL germline sequence. In some embodiments, librariescontaining a single light chain germline sequence and its variants areprovided. In certain embodiments, variants produced from different lightchain germline sequences can be combined to produce libraries encodingmultiple light chain germline sequences and their variants. Any of thelight chain L3-VL germline sequences provided herein may be varied attwo or three residues between positions 89 to 94, inclusive, and one ofordinary skill in the art will recognize that any other L3-VL sequencecan also be varied according to the principles described herein toproduce libraries provided by the invention. In some embodiments, thepresent invention comprises libraries containing polynucleotides thatencode antibody light chain variable regions, wherein the antibody lightchain variable regions comprise one or more of the following L3-VLsequences: (i) an amino acid sequence that is identical to an L3-VLgermline sequence (e.g., see Table 1); (ii) an amino acid sequence thatcontains two substitutions between residues 89-94, inclusive, incomparison to an L3-VL germline sequence; and (iii) an amino acidsequence that contains three substitutions between residues 89-94,inclusive, in comparison to an L3-VL germline sequence. In someembodiments, each antibody light chain variable region on a libraryincludes one or more of the above L3-VL sequences. In some embodiments,such a library is combined with one or more sets of other nucleic acidsthat may or may not encode antibody light chain variable regions, andmay or may not contain such L3-VL sequences. In some embodiments, thepresent invention comprises libraries containing polynucleotides thatencode an antibody light chain variable region having an amino acidsequence as set forth in Table 4, or a polynucleotide sequence as setforth in one or more of Tables 5-7, wherein two or three residues atpositions 89-94, inclusive, are varied.

In some embodiments, the present invention comprises librariescontaining polynucleotides that encode an antibody light chain variableregion, wherein, across the library, all encoded antibody light chainvariable regions are identical to one another except for substitutionsof residues at positions between residue 89 and residue 94, inclusiveand further wherein, across the library, sequences of any two encodedantibody light chain variable regions differ from one another at notmore than 3 positions.

In some embodiments, the invention provides libraries comprisingpolynucleotides encoding light chain variable regions comprisingpolypeptide sequences at least about 80%, 90%, or 95% identical topolypeptides produced by translation of two or more of thepolynucleotide sequences provided in Tables 5-7. In certain embodimentsall members of the library are at least about 80%, 90%, or 95% identicalto polypeptides produced by translation of two or more of thepolynucleotide sequences provided in Tables 5-7.

In certain embodiments, the invention provides a library comprisinglight chain variable regions that comprise the polypeptides produced bytranslation of the polynucleotide sequences provided in Tables 5-7. Incertain embodiments, all members of the library comprise thepolypeptides produced by translation of the polynucleotide sequencesprovided in Tables 5-7.

In some embodiments, any of the libraries described herein as containingor encoding CDRL3 and/or light chain variable regions, contains orencodes such CDRL3 and/or light chain variable regions in the context ofcomplete light chains. Furthermore, in some embodiments, such libraries(and/or complete light chain libraries) further contain or encode one ormore heavy chain CDRH3, variable domains, or intact heavy chains. Insome embodiments, provided libraries include or encode intact antibodiessuch as, for example, intact IgGs.

In some embodiments, provided libraries include or encode humanantibodies or antibody fragments; in some such embodiments, providedlibraries include or encode intact human antibodies.

In certain embodiments, the invention provides libraries that comprisenucleic acid vectors containing library nucleic acids described herein.In many embodiments, each such library member comprises the same vector.

In some embodiments, the invention provides host cells containing one ormore provided libraries, for example including a vector. In someembodiments the host cell is a yeast, and in certain embodiments theyeast is Saccharomyces cerevisiae.

In some embodiments, the invention provides antibodies isolated from thelibraries described herein.

In certain embodiments, the invention provides kits containing any ofthe libraries described herein.

In some embodiments, the invention provides representations of librariesand/or theoretical segment pools in a computer readable format, forexample, the TN1 polypeptides of Tables 10, 23-25 and 26; the DHpolypeptides of Tables 11, 23-25 and 28; the N2 polypeptides of Tables12, 23-25 and 30; the H3-JH polypeptides of Tables 13, 15, 17, 23-25 and32; the TN1 polynucleotides of Tables 25-26; the DH polynucleotides ofTables 25 and 27; the N2 polynucleotides of Tables 25 and 29; and/or theH3-JH polynucleotides of Tables 25 and 31.

In certain embodiments, the invention provides a representation of thepolynucleotide sequences of the Human Preimmune Set (Appendix A), or thepolypeptide expression products thereof, in a computer readable format.

In some embodiments, the invention provides a method of making syntheticpolynucleotides encoding a CDRH3 library, comprising: (a) providing atheoretical segment pool containing TN1, DH, N2, and H3-JH segments; (b)providing a reference set of CDRH3 sequences; (c) utilizing thetheoretical segment pool of (a) to identify the closest match(es) toeach CDRH3 sequence in the reference set of (b); (d) selecting segmentsfrom the theoretical segment pool for inclusion in a synthetic library;and (e) synthesizing the synthetic CDRH3 library. In certainembodiments, the invention provides libraries made by this method. Insome embodiments, the segments selected for inclusion in the syntheticlibrary are selected according to their segment usage weight in thereference set of CDRH3 sequences.

In certain embodiments, the invention provides a method of makingsynthetic polynucleotides encoding a CDRL3 library, comprising: (i)obtaining a reference set of light chain sequences, wherein thereference set contains light chain sequences with VL segmentsoriginating from the same IGVL germline gene and/or its allelicvariants; (ii) determining which amino acids occur at each of the CDRL3positions in the reference set that are encoded by the IGVL gene; (iii)synthesizing light chain variable domain encoding sequences wherein twopositions between positions 89 and 94, inclusive, contain degeneratecodons encoding two or more of the five most frequently occurring aminoacid residues at the corresponding positions in the reference set; and(iv) synthesizing the polynucleotides encoding the CDRL3 library. Incertain embodiments, the invention provides libraries made by thismethod.

In some embodiments, the invention provides a method of using any of thelibraries of the invention to isolate an antibody binding an antigen,comprising contacting the polypeptide expression products of saidlibraries with an antigen and isolating polypeptide expression productsthat bind to the antigen.

In certain embodiments, the number of N-linked glycosylation sites,deamidation motifs, and/or Cys residues in the libraries of theinvention are reduced or eliminated in comparison to libraries producedby amplification of a repertoire from a biological source.

The invention provides a number of polynucleotide and polypeptidesequences and segments that can be used to build larger polynucleotideand polypeptide sequences (e.g., TN1, DH, N2, and H3-JH segments thatcan be used to build CDRH3). One of ordinary skill in the art willreadily recognize that in some instances these sequences can be moresuccinctly represented by providing consensus sequences after alignmentof the sequences provided by the invention, and that these consensussequences fall within the scope of the invention and may be used to moresuccinctly represent any of the sequences provided herein.

BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 shows that Vernier residues 4 and 49 (starred) in VK1-39 have adiversity index comparable to or greater than the diversity indices ofthe CDR positions (i.e., at or above 0.07, in this example).

FIG. 2 shows that clinically validated CDRL3 sequences deviate littlefrom germline-like sequences (n=35).

FIG. 3 shows the percent of sequences in the jumping dimer CDRL3libraries of the invention and a previous CDRL3 library, VK-v1.0, with Xor fewer mutations from germline. Here, FX is the percentage ofsequences in a library with X or fewer mutations from germline.

FIG. 4 shows the application of a provided method used to generatenucleotide sequences (SEQ ID NOS 8748-8759, respectively, in order ofappearance) encoding the parent H3-JH segments.

FIG. 5 shows the general schematic of an approach used to selectsegments from a theoretical segment pool for inclusion in a theoreticaland/or synthetic library.

FIG. 6 shows the frequency of “Good” and “Poor” expressing CDRH3sequences isolated from yeast-based libraries described in US2009/0181855, and their comparison to the sequences contained in thelibrary design described therein (“Design”), as a function of the DHsegment hydrophobicity (increasing to the right).

FIG. 7 shows the percentage of CDRH3 sequences in the LUA-141 libraryand Exemplary Library Design 3 (ELD-3) that match CDRH3 sequences fromLee-666 and Boyd-3000 with zero, one, two, three, or more than threeamino acid mismatches.

FIG. 8 shows that Exemplary Library Design 3 (ELD-3) and the ExtendedDiversity Library Design both return better matches to clinicallyrelevant CDRH3 sequences than the LUA-141 library.

FIG. 9 shows that the combinatorial efficiency of Exemplary LibraryDesign 3 (ELD-3) is greater than that of the LUA-141 library.Specifically, the ELD-3 segments are more likely to yield a unique CDRH3than the LUA-141 library segments.

FIG. 10 shows the amino acid compositions of the Kabat-CDRH3s ofLUA-141, Exemplary Library Design 3 (ELD-3), and Human CDRH3 sequencesfrom the HPS (Human H3).

FIG. 11 shows the Kabat-CDRH3 length distribution of LUA-141, ExemplaryLibrary Design 3 (ELD-3), and Human CDRH3 sequences from the HPS (HumanH3).

FIG. 12 shows the percentage of CDRH3 sequences in the ExtendedDiversity library that match CDRH3 sequences from Boyd et al. with zeroto thirty-two amino acid mismatches

FIG. 13 shows the Kabat-CDRH3 length distribution of Exemplary LibraryDesign 3 (“ELD-3”), the Extended Diversity Library Design (“ExtendedDiversity”) and human CDRH3 sequences from the Boyd et al. data set(“Boyd 2009”).

FIG. 14 shows the amino acid compositions of the Kabat-CDRH3s of theExtended Diversity Library Design (“Extended Diversity”) and human CDRH3sequences from the Boyd et al. dataset (“Boyd 2009”).

FIG. 15 shows the combinatorial efficiency of the Extended DiversityLibrary Design by matching 20,000 randomly selected sequences from the(same) design. About 65% of the sequences appear only once in the designand about 17% appear twice.

DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS OF THE INVENTION

The present invention provides, among other things, polynucleotide andpolypeptide libraries, methods of producing and using the libraries,kits containing the libraries, and computer readable forms ofrepresentations of libraries and/or theoretical segment pools disclosedherein. Libraries taught in this application can be described, at leastin part, in terms of components (e.g., polynucleotide or polypeptide“segments”) from which they are assembled. Among other things, thepresent invention specifically provides and contemplates thesepolynucleotide or polypeptide segments, methods of producing and usingsuch segments, and kits and computer readable forms of representationsthat include such library segments.

In certain embodiments, the invention provides antibody librariesspecifically designed based on sequences and CDR length distribution ina naturally occurring human antibody repertoire. It is estimated that,even in the absence of antigenic stimulation, an individual human makesat least about 10⁷ different antibody molecules (Boyd et al., ScienceTranslational Medicine, 2009, 1: 1). The antigen-binding sites of manyantibodies can cross-react with a variety of related but differentepitopes. In addition, the human antibody repertoire is large enough toensure that there is an antigen-binding site to fit almost any potentialepitope, albeit potentially with low affinity.

The mammalian immune system has evolved unique genetic mechanisms thatenable it to generate an almost unlimited number of different light andheavy chains in a remarkably economical way, by combinatorially joiningchromosomally separated gene segments prior to transcription. Each typeof immunoglobulin (Ig) chain (i.e., kappa light, lambda light, andheavy) is synthesized by combinatorial assembly of DNA sequences,selected from two or more families of gene segments, to produce a singlepolypeptide chain. Specifically, the heavy chains and light chains eachconsist of a variable region and a constant (C) region. The variableregions of the heavy chains are encoded by DNA sequences assembled fromthree families of gene sequences: variable (IGHV), diversity (IGHD), andjoining (IGHJ). The variable regions of light chains are encoded by DNAsequences assembled from two families of gene sequences for each of thekappa and lambda light chains: variable (IGLV) and joining (IGLJ). Eachvariable region (heavy and light) is also recombined with a constantregion, to produce a full-length immunoglobulin chain.

While combinatorial assembly of the V, D and J gene segments make asubstantial contribution to antibody variable region diversity, furtherdiversity is introduced in vivo, at the pre-B cell stage, via imprecisejoining of these gene segments and the introduction of non-templatednucleotides at the junctions between the gene segments (see e.g., U.S.Pub. No. 2009/0181855, which is incorporated by reference in itsentirety, for more information).

After a B cell recognizes an antigen, it is induced to proliferate.During proliferation, the B cell receptor locus undergoes an extremelyhigh rate of somatic mutation that is far greater than the normal rateof genomic mutation. The mutations that occur are primarily localized tothe Ig variable regions and comprise substitutions, insertions anddeletions. This somatic hypermutation enables the production of B cellsthat express antibodies possessing enhanced affinity toward an antigen.Such antigen-driven somatic hypermutation fine-tunes antibody responsesto a given antigen.

Synthetic antibody libraries of the instant invention have the potentialto recognize any antigen, including antigens of human origin. Theability to recognize antigens of human origin may not be present inother antibody libraries, such as antibody libraries prepared from humanbiological sources (e.g., from human cDNA), because self-reactiveantibodies are removed by the donor's immune system via negativeselection.

Still further, the present invention provides strategies that streamlineand/or simplify certain aspects of library development and/or screening.For example, in some embodiments, the present invention permits use ofcell sorting technologies (e.g., fluorescence activated cell sorting,FACS) to identify positive clones, and therefore bypasses or obviatesthe need for the standard and tedious methodology of generating ahybridoma library and supernatant screening.

Yet further, in some embodiments, the present invention provideslibraries and/or sublibraries that accommodate multiple screeningpasses. For example, in some embodiments, provided libraries and/orsublibraries can be screened multiple times. In some such embodiments,individual provided libraries and/or sublibraries can be used todiscover additional antibodies against many targets.

Before further description of the invention, certain terms are defined.

Definitions

Unless defined otherwise, all technical and scientific terms used hereinhave the meaning commonly understood by one of ordinary skill in the artrelevant to the invention. Unless otherwise specified, the Kabatnumbering system is used throughout the application. The definitionsbelow supplement those in the art and are directed to the embodimentsdescribed in the current application.

The term “amino acid” or “amino acid residue,” as would be understood byone of ordinary skill in the art, typically refers to an amino acidhaving its art recognized definition such as an amino acid selected fromthe group consisting of: alanine (Ala or A); arginine (Arg or R);asparagine (Asn or N); aspartic acid (Asp or D); cysteine (Cys or C);glutamine (Gln or Q); glutamic acid (Glu or E); glycine (Gly or G);histidine (H is or H); isoleucine (Ile or I): leucine (Leu or L); lysine(Lys or K); methionine (Met or M); phenylalanine (Phe or F); proline(Pro or P); serine (Ser or S); threonine (Thr or T); tryptophan (Trp orW); tyrosine (Tyr or Y); and valine (Val or V), although modified,synthetic, or rare amino acids may be used as desired. Generally, aminoacids can be grouped as having a non-polar side chain (e.g., Ala, Cys,Ile, Leu, Met, Phe, Pro, Val); a negatively charged side chain (e.g.,Asp, Glu); a positively charged side chain (e.g., Arg, His, Lys); or anuncharged polar side chain (e.g., Asn, Cys, Gln, Gly, His, Met, Phe,Ser, Thr, Trp, and Tyr).

As would be understood by those of ordinary skill in the art, the term“antibody” is used herein in the broadest sense and specificallyencompasses at least monoclonal antibodies, polyclonal antibodies,multi-specific antibodies (e.g., bispecific antibodies), chimericantibodies, humanized antibodies, human antibodies, and antibodyfragments. An antibody is a protein comprising one or more polypeptidessubstantially or partially encoded by immunoglobulin genes or fragmentsof immunoglobulin genes. The recognized immunoglobulin genes include thekappa, lambda, alpha, gamma, delta, epsilon and mu constant regiongenes, as well as myriad immunoglobulin variable region genes.

The term “antibody binding region” refers to one or more portions of animmunoglobulin or antibody variable region capable of binding anantigen(s). Typically, the antibody binding region is, for example, anantibody light chain (or variable region or one or more CDRs thereof),an antibody heavy chain (or variable region or one or more CDRsthereof), a heavy chain Fd region, a combined antibody light and heavychain (or variable regions thereof) such as a Fab, F(ab′)₂, singledomain, or single chain antibodies (scFv), or any region of a fulllength antibody that recognizes an antigen, for example, an IgG (e.g.,an IgG1, IgG2, IgG3, or IgG4 subtype), IgA1, IgA2, IgD, IgE, or IgMantibody.

“Antibody fragments” comprise a portion of an intact antibody, forexample, one or more portions of the antigen-binding region thereof.Examples of antibody fragments include Fab, Fab′, F(ab′)₂, and Fvfragments, diabodies, linear antibodies, single-chain antibodies, andmulti-specific antibodies formed from intact antibodies and antibodyfragments.

The term “antibody of interest” refers to an antibody that has aproperty of interest that is identified and/or isolated from a libraryof the invention. Exemplary properties of interest include, for example,but are not limited to, binding to a particular antigen or epitope,binding with a certain affinity, cross-reactivity, blocking a bindinginteraction between two molecules, and/or eliciting a certain biologicaleffect.

The term “canonical structure,” as understood by those of ordinary skillin the art, refers to the main chain conformation that is adopted by theantigen binding (CDR) loops. From comparative structural studies, it hasbeen found that five of the six antigen binding loops have only alimited repertoire of available conformations. Each canonical structurecan be characterized by the torsion angles of the polypeptide backbone.Correspondent loops between antibodies may, therefore, have very similarthree dimensional structures, despite high amino acid sequencevariability in most parts of the loops (Chothia and Lesk, J. Mol. Biol.,1987, 196: 901; Chothia et al., Nature, 1989, 342: 877; Martin andThornton, J. Mol. Biol., 1996, 263: 800, each of which is incorporatedby reference in its entirety). Furthermore, there is a relationshipbetween the adopted loop structure and the amino acid sequencessurrounding it. As is known in the art, the conformation of a particularcanonical class is determined by the length of the loop and the aminoacid residues residing at key positions within the loop, as well aswithin the conserved framework (i.e., outside of the loop). Assignmentto a particular canonical class can therefore be made based on thepresence of these key amino acid residues. The term “canonicalstructure” may also include considerations as to the linear sequence ofthe antibody, for example, as catalogued by Kabat (Kabat et al., in“Sequences of Proteins of Immunological Interest,” 5^(th) Edition, U.S.Department of Heath and Human Services, 1992). The Kabat numberingscheme is a widely adopted standard for numbering the amino acidresidues of an antibody variable domain in a consistent manner, and isused herein unless indicated otherwise. Additional structuralconsiderations can also be used to determine the canonical structure ofan antibody. For example, those differences not fully reflected by Kabatnumbering can be described by the numbering system of Chothia et al.and/or revealed by other techniques, for example, crystallography andtwo or three-dimensional computational modeling. Accordingly, a givenantibody sequence may be placed into a canonical class which allows for,among other things, identifying appropriate chassis sequences (e.g.,based on a desire to include a variety of canonical structures in alibrary). Kabat numbering of antibody amino acid sequences andstructural considerations as described by Chothia et al., and theirimplications for construing canonical aspects of antibody structure, aredescribed in the literature.

The terms “CDR”, and its plural “CDRs”, refer to a complementaritydetermining region (CDR) of which three make up the binding character ofa light chain variable region (CDRL1, CDRL2 and CDRL3) and three make upthe binding character of a heavy chain variable region (CDRH1, CDRH2 andCDRH3). CDRs contribute to the functional activity of an antibodymolecule and are separated by amino acid sequences that compriseframework regions. The exact definitional CDR boundaries and lengths aresubject to different classification and numbering systems. CDRs maytherefore be referred to by Kabat, Chothia, contact or other boundarydefinitions, including for example the CDRH3 numbering system describedbelow. Despite differing boundaries, each of these systems has somedegree of overlap in what constitutes the so called “hypervariableregions” within the variable region. CDR definitions according to thesesystems may therefore differ in length and boundary areas with respectto the adjacent framework region. See, for example Kabat et al., in“Sequences of Proteins of Immunological Interest,” 5^(th) Edition, U.S.Department of Health and Human Services, 1992; Chothia et al., J. Mol.Biol., 1987, 196: 901; and MacCallum et al., J. Mol. Biol., 1996, 262:732, each of which is incorporated by reference in its entirety.

The “CDRH3 numbering system” used herein defines the first amino acid ofCDRH3 as being at position 95 and the last amino acid of CDRH3 asposition 102. Note that this is a custom numbering system that is notaccording to Kabat. The amino acid segment, beginning at position 95 iscalled “TN1” and, when present, is assigned numbers 95, 96, 96A, 96B,etc. Note that the nomenclature used in the current application isslightly different from that used in U.S. Publication Nos. 2009/0181855and 2010/0056386, and WO/2009/036379. In those applications, position 95was designated a “Tail” residue, while here, the Tail (T) has beencombined with the N1 segment, to produce one segment, designated “TN1.”The TN1 segment is followed by the “DH” segment, which is assignednumbers 97, 97A, 97B, 97C, etc. The DH segment is followed by the “N2”segment, which, when present, is numbered 98, 98A, 98B, etc. Finally,the most C-terminal amino acid residue of the “H3-JH” segment isdesignated as number 102. The residue directly before (N-terminal) it,when present, is 101, and the one before (if present) is 100. The restof the H3-JH amino acids are numbered in reverse order, beginning with99 for the amino acid just N-terminal to 100, 99A for the residueN-terminal to 99, and so forth for 99B, 99C, etc. Examples of CDRH3sequence residue numbers may therefore include the following:

“Chassis” of the invention are portions of the antibody heavy chainvariable (IGHV) or light chain variable (IGLV) domains that are not partof CDRH3 or CDRL3, respectively. A chassis of the invention is definedas the portion of the variable region of an antibody beginning with thefirst amino acid of FRM1 and ending with the last amino acid of FRM3. Inthe case of the heavy chain, the chassis includes the amino acidsincluding from position 1 to position 94. In the case of the lightchains (kappa and lambda), the chassis are defined as including fromposition 1 to position 88. The chassis of the invention may containcertain modifications relative to the corresponding germline variabledomain sequences. These modifications may be engineered (e.g., to removeN-linked glycosylation sites) or naturally occurring (e.g., to accountfor naturally occurring allelic variation). For example, it is known inthe art that the immunoglobulin gene repertoire is polymorphic (Wang etal., Immunol. Cell. Biol., 2008, 86: 111; Collins et al.,Immunogenetics, 2008, 60: 669, each incorporated by reference in itsentirety); chassis, CDRs and constant regions representative of theseallelic variants are also encompassed by the invention. In someembodiments, the allelic variant(s) used in a particular embodiment ofthe invention may be selected based on the allelic variation present indifferent patient populations, for example, to identify antibodies thatare non-immunogenic in these patient populations. In certainembodiments, the immunogenicity of an antibody of the invention maydepend on allelic variation in the major histocompatibility complex(MHC) genes of a patient population. Such allelic variation may also beconsidered in the design of libraries of the invention. In certainembodiments of the invention, the chassis and constant regions arecontained in a vector, and a CDR3 region is introduced between them viahomologous recombination.

As used herein, a sequence designed with “directed diversity” has beenspecifically designed to contain both sequence diversity and lengthdiversity. Directed diversity is not stochastic.

As used herein, the term “diversity” refers to a variety or a noticeableheterogeneity. The term “sequence diversity” refers to a variety ofsequences which are collectively representative of several possibilitiesof sequences, for example, those found in natural human antibodies. Forexample, CDRH3 sequence diversity may refer to a variety ofpossibilities of combining the known human TN1, DH, N2, and H3-JHsegments to form CDRH3 sequences. The CDRL3 sequence diversity (kappa orlambda) may refer to a variety of possibilities of combining thenaturally occurring light chain variable region contributing to CDRL3(i.e., “L3-VL”) and joining (i.e., “L3-JL”) segments, to form CDRL3sequences. As used herein, “H3-JH” refers to the portion of the IGHJgene contributing to CDRH3. As used herein, “L3-VL” and “L3-JL” refer tothe portions of the IGLV and IGLJ genes (kappa or lambda) contributingto CDRL3, respectively.

As used herein, the term “expression” refers to steps involved in theproduction of a polypeptide including, but not limited to,transcription, post-transcriptional modification, translation,post-translational modification, and secretion.

The term “framework region” refers to the art-recognized portions of anantibody variable region that exist between the more divergent (i.e.,hypervariable) CDRs. Such framework regions are typically referred to asframeworks 1 through 4 (FRM1, FRM2, FRM3, and FRM4) and provide ascaffold for the presentation of the six CDRs (three from the heavychain and three from the light chain) in three dimensional space, toform an antigen-binding surface.

The term “full-length heavy chain” refers to an immunoglobulin heavychain that contains each of the canonical structural domains of animmunoglobulin heavy chain, including the four framework regions, thethree CDRs, and the constant region.

The term “full-length light chain” refers to an immunoglobulin lightchain that contains each of the canonical structural domains of animmunoglobulin light chain, including the four framework regions, thethree CDRs, and the constant region.

The term “germline-like,” when used with respect to the CDRL3 sequencesof the light chains of the invention, means those sequences consistingof combinations of: (i) the first six wild-type residues contributed toCDRL3 by the IGVL germline gene (i.e., positions 89 to 94 in the Kabatnumbering system; “L” is kappa or lambda); and (ii) one of several aminoacid sequences, two one to four amino acids in length, largely, but notexclusively, derived from the JL segment (“L,” again is kappa orlambda). For kappa CDRL3 sequences of the most common lengths (i.e., 8,9, and 10 residues), the sequences of (ii) number twenty and are: FT,LT, IT, RT, WT, YT, [X]T, [X]PT, [X]FT, [X]LT, [X]IT, [X]RT, [X]WT,[X]YT, [X]PFT, [X]PLT, [X]PIT, [X]PRT, [X]PWT and [X]PYT, where[X]corresponds to the amino acid residue found at position 95 (Kabat) inthe respective VK germline sequence. X is most commonly P, but may alsobe S or any other amino acid residue found at position 95 of a VKgermline sequence. For eight exemplified VK chassis exemplified herein,the corresponding 160 germline-like sequences, (i.e., 20 sequences oftwo to four amino acids in length combined with positions 89 to 94 ofeach of eight VK germline sequences) are provided in Table 1. A similarapproach is applied to define germline-like CDRL3 sequences for lambdalight chains. As for the kappa sequences described above, the intact,un-mutated portions of CDRL3 encoded by the IGVL genes (in this case,IGVλ) would be combined with the sequences largely, but not exclusively,derived from the Jλ segment. Here, the latter sequences (correspondingto (ii), above), number five and are: YV, VV, WV, AV or V. In addition,and as described in Example 7 of US 2009/0818155, one could furtherallow for variation at the last position of the Vλ-gene-encoded portionof CDRL3 by considering partial codons, while still considering theresulting sequences “germline-like.” More specifically, the entire“minimalist library” of Example 7 in US 2009/0818155 would be defined as“germline-like.” One of ordinary skill in the art will readily recognizethat these methods can be extended to other VK and Vλ sequences.

The term “genotype-phenotype linkage,” as understood by those ofordinary skill in the art, refers to the fact that the nucleic acid(genotype) encoding a protein with a particular phenotype (e.g., bindingan antigen) can be isolated from a library. For the purposes ofillustration, an antibody fragment expressed on the surface of a phagecan be isolated based on its binding to an antigen (e.g., U.S. Pat. No.5,837,500). The binding of the antibody to the antigen simultaneouslyenables the isolation of the phage containing the nucleic acid encodingthe antibody fragment. Thus, the phenotype (antigen-bindingcharacteristics of the antibody fragment) has been “linked” to thegenotype (nucleic acid encoding the antibody fragment). Other methods ofmaintaining a genotype-phenotype linkage include those of Wittrup et al.(U.S. Pat. Nos. 6,300,065, 6,331,391, 6,423,538, 6,696,251, 6,699,658,and U.S. Pub. No. 20040146976, each of which is incorporated byreference in its entirety), Miltenyi (U.S. Pat. No. 7,166,423,incorporated by reference in its entirety), Fandl (U.S. Pat. No.6,919,183, US Pub No. 20060234311, each incorporated by reference in itsentirety), Clausell-Tormos et al. (Chem. Biol., 2008, 15: 427,incorporated by reference in its entirety), Love et al. (Nat.Biotechnol., 2006, 24: 703, incorporated by reference in its entirety),and Kelly et al. (Chem. Commun., 2007, 14: 1773, incorporated byreference in its entirety). The term can be used to refer to any methodwhich localizes an antibody protein together with the gene encoding theantibody protein, in a way in which they can both be recovered while thelinkage between them is maintained.

The term “heterologous moiety” is used herein to indicate the additionof a moiety to an antibody wherein the moiety is not part of anaturally-occurring antibody. Exemplary heterologous moieties includedrugs, toxins, imaging agents, and any other compositions which mightprovide an activity that is not inherent in the antibody itself.

As used herein, the term “host cell” is intended to refer to a cellcomprising a polynucleotide of the invention. It should be understoodthat such terms refer not only to the particular subject cell but to theprogeny or potential progeny of such a cell. Because certainmodifications may occur in succeeding generations due to either mutationor environmental influences, such progeny may not, in fact, be identicalto the parent cell, but are still included within the scope of the termas used herein.

As used herein, the term “human antibody CDR library” includes at leasta polynucleotide or polypeptide library which has been designed torepresent the sequence diversity and length diversity of naturallyoccurring CDRs in human antibodies (e.g., the term “CDR” in “humanantibody CDR library” may be substituted with “CDRL1,” “CDRL2,” “CDRL3,”“CDRH1,” “CDRH2,” and/or “CDRH3”). Known human CDR sequences arerepresented in various data sets, including Jackson et al., J. Immunol.Methods, 2007, 324: 26; Martin, Proteins, 1996, 25: 130; Lee et al.,Immunogenetics, 2006, 57: 917, Boyd et al., Science TranslationalMedicine, 2009, 1: 1, and WO/2009/036379, each of which is incorporatedby reference in its entirety, and the HPS, which is provided in AppendixA.

The term “Human Preimmune Set,” or “HPS,” refers to a reference set of3,571 curated human preimmune heavy chain sequences corresponding to theGI Nos. provided in Appendix A.

An “intact antibody” is one comprising full-length heavy- andlight-chains (i.e., four frameworks, three CDRs, and a constant regionfor each of the heavy and light chains). An intact antibody is alsoreferred to as a “full-length” antibody.

The term “length diversity” refers to a variety in the length of afamily of nucleotide or amino acid sequence. For example, in naturallyoccurring human antibodies, the heavy chain CDR3 sequence varies inlength, for example, from about 2 amino acids to over about 35 aminoacids, and the light chain CDR3 sequence varies in length, for example,from about 5 to about 16 amino acids.

The term “library” refers to a set of entities comprising two or moreentities having diversity as described herein, and/or designed accordingto the methods of the invention. For example, a “library ofpolynucleotides” refers to a set of polynucleotides comprising two ormore polynucleotides having diversity as described herein, and/ordesigned according to the methods of the invention. A “library ofpolypeptides” refers to a set of polypeptides comprising two or morepolypeptides having diversity as described herein, and/or designedaccording to the methods of the invention. A “library of syntheticpolynucleotides” refers to a set of polynucleotides comprising two ormore synthetic polynucleotides having diversity as described herein,and/or designed according to the methods of the invention. Librarieswhere all members are synthetic are also encompassed by the invention. A“human antibody library” refers to a set of polypeptides comprising twoor more polypeptides having diversity as described herein, and/ordesigned according to the methods of the invention, for example alibrary designed to represent the sequence diversity and lengthdiversity of naturally occurring human antibodies. In some embodiments,the term “library” may refer to a set of entities sharing similarstructural or sequence characteristics, for example, a “heavy chainlibrary,” “light chain library,” “antibody library,” and/or “CDRH3library.”

The term “physical realization” refers to a portion of a theoretical(e.g., computer-based) or synthetic (e.g., oligonucleotide-based)diversity that can actually be physically sampled, for example, by anydisplay methodology. Exemplary display methodology include: phagedisplay, ribosomal display, and yeast display. For synthetic sequences,the size of the physical realization of a library depends on (1) thefraction of the theoretical diversity that can actually be synthesized,and (2) the limitations of the particular screening method. Exemplarylimitations of screening methods include the number of variants that canbe screened in a particular assay (e.g., ribosome display, phagedisplay, yeast display) and the transformation efficiency of the hostcells (e.g., yeast, mammalian cells, bacteria) which are used in ascreening assay. For the purposes of illustration, given a library witha theoretical diversity of 10¹² members, an exemplary physicalrealization of the library (e.g., in yeast, bacterial cells, or ribosomedisplay) that can maximally include 10¹¹ members will, therefore, sampleabout 10% of the theoretical diversity of the library. However, if fewerthan 10¹¹ members of the library with a theoretical diversity of 10¹²are synthesized, and the physical realization of the library canmaximally include 10¹¹ members, less than 10% of the theoreticaldiversity of the library is sampled in the physical realization of thelibrary. Similarly, a physical realization of the library that canmaximally include more than 10¹² members would “oversample” thetheoretical diversity, meaning that each member may be present more thanonce (assuming that the entire 10¹² theoretical diversity issynthesized).

The term “polynucleotide(s)” refers to nucleic acids such as DNAmolecules and RNA molecules and analogs thereof (e.g., DNA or RNAgenerated using nucleotide analogs or using nucleic acid chemistry). Asdesired, the polynucleotides may be made synthetically, e.g., usingart-recognized nucleic acid chemistry or enzymatically using, e.g., apolymerase, and, if desired, be modified. Typical modifications includemethylation, biotinylation, and other art-known modifications. Inaddition, the nucleic acid molecule can be single-stranded ordouble-stranded and, where desired, linked to a detectable moiety. Therepresentation of nucleotide bases herein follows International Union ofPure and Applied Chemistry (IUPAC) nomenclature (see U.S. Pub. No.2009/0181855, incorporated by reference in its entirety).

“Preimmune” antibody libraries have sequence diversities and lengthdiversities similar to naturally occurring human antibody sequencesbefore these sequences have undergone negative selection and/or somatichypermutation. For example, the set of sequences described in Lee et al.(Immunogenetics, 2006, 57: 917, incorporated by reference in itsentirety) and the Human Preimmune Set (HPS) described herein (seeAppendix A) are believed to represent sequences from the preimmunerepertoire. In certain embodiments of the invention, the sequences ofthe invention will be similar to these sequences (e.g., in terms ofcomposition and length).

As used herein, the term “sitewise stochastic” describes a process ofgenerating a sequence of amino acids, where only the amino acidoccurrences at the individual positions are considered, and higher ordermotifs (e.g., pair-wise correlations) are not accounted for (e.g., seeKnappik, et al., J Mol Biol, 2000, 296: 57, and analysis provided inU.S. Publication No. 2009/0181855, each incorporated by reference in itsentirety).

The term “split-pool synthesis” refers to a procedure in which theproducts of a plurality of individual first reactions are combined(pooled) and then separated (split) before participating in a pluralityof second reactions. For example, U.S. Publication No. 2009/0181855(incorporated by reference in its entirety) describes the synthesis of278 DH segments (products), each in a separate reaction. Aftersynthesis, these 278 segments are combined (pooled) and then distributed(split) amongst 141 columns for the synthesis of the N2 segments. Thisenables the pairing of each of the 278 DH segments with each of 141 N2segments.

As used herein, “stochastic” describes a process of generating a randomsequence of nucleotides or amino acids, which is considered as a sampleof one element from a probability distribution (e.g., see U.S. Pat. No.5,723,323).

As used herein, the term “synthetic polynucleotide” refers to a moleculeformed through a chemical process, as opposed to molecules of naturalorigin, or molecules derived via template-based amplification ofmolecules of natural origin (e.g., immunoglobulin chains cloned frompopulations of B cells via PCR amplification are not “synthetic” as usedherein). In some instances, for example, when referring to libraries ofthe invention that comprise multiple segments (e.g., TN1, DH, N2, and/orH3-JH), the invention encompasses libraries in which at least one, two,three, or four of the aforementioned components is synthetic. By way ofillustration, a library in which certain components are synthetic, whileother components are of natural origin or derived via template-basedamplification of molecules of natural origin, would be encompassed bythe invention. Libraries that are fully synthetic would, of course, alsobe encompassed by the invention.

The term “theoretical diversity” refers to the maximum number ofvariants in a library design. For example, given an amino acid sequenceof three residues, where residues one and three may each be any one offive amino acid types and residue two may be any one of 20 amino acidtypes, the theoretical diversity is 5×20×5=500 possible sequences.Similarly if sequence X is constructed by combination of 4 amino acidsegments, where segment 1 has 100 possible sequences, segment 2 has 75possible sequences, segment 3 has 250 possible sequences, and segment 4has 30 possible sequences, the theoretical diversity of fragment X wouldbe 100×75×200×30, or 5.6×10⁵ possible sequences.

The term “theoretical segment pool” refers to a set of polynucleotide orpolypeptide segments that can be used as building blocks to assemble alarger polynucleotide or polypeptide. For example, a theoretical segmentpool containing TN1, DH, N2, and H3-JH segments can be used to assemblea library of CDRH3 sequences by concatenating them combinatorially toform a sequence represented by [TN1]-[DH]-[N2]-[H3-JH], and synthesizingthe corresponding oligonucleotide(s). The term “theoretical segmentpool” can apply to any set of polynucleotide or polypeptide segments.Thus, while a set of TN1, DH, N2, and H3-JH segments are collectivelyconsidered a theoretical segment pool, each of the individual sets ofsegments also comprise a theoretical segment pool, specifically a TN1theoretical segment pool, a DH theoretical segment pool, an N2theoretical segment pool, and an H3-JH theoretical segment pool. Anysubsets of these theoretical segment pools containing two or moresequences can also be considered theoretical segment pools.

The term “unique,” as used herein, refers to a sequence that isdifferent (e.g., has a different chemical structure) from every othersequence within the designed set (e.g., the theoretical diversity). Itshould be understood that there are likely to be more than one copy ofmany unique sequences from the theoretical diversity in a particularphysical realization. For example, a library comprising three uniquesequences at the theoretical level may comprise nine total members ifeach sequence occurs three times in the physical realization of thelibrary. However, in certain embodiments, each unique sequence may occuronly once, less than once, or more than once.

The term “variable” refers to the portions of the immunoglobulin domainsthat exhibit variability in their sequence and that are involved indetermining the specificity and binding affinity of a particularantibody (i.e., the “variable domain(s)”). Variability is not evenlydistributed throughout the variable domains of antibodies; it isconcentrated in sub-domains of each of the heavy and light chainvariable regions. These sub-domains are called “hypervariable” regionsor “complementarity determining regions” (CDRs). The more conserved(i.e., non-hypervariable) portions of the variable domains are calledthe “framework” regions (FRM). The variable domains of naturallyoccurring heavy and light chains each comprise four FRM regions, largelyadopting a 13-sheet configuration, connected by three hypervariableregions, which form loops connecting, and in some cases forming part of,the 13-sheet structure. The hypervariable regions in each chain are heldtogether in close proximity by the FRM and, with the hypervariableregions from the other chain, contribute to the formation of theantigen-binding site (see Kabat et al. Sequences of Proteins ofImmunological Interest, 5th Ed. Public Health Service, NationalInstitutes of Health, Bethesda, Md., 1991, incorporated by reference inits entirety). The constant domains are not directly involved in antigenbinding, but exhibit various effector functions, such as, for example,antibody-dependent, cell-mediated cytotoxicity and complementactivation.

Libraries of the invention containing “VKCDR3” sequences and “VλCDR3”sequences refer to the kappa and lambda sub-sets of the light chain CDR3(CDRL3) sequences, respectively. Such libraries may be designed withdirected diversity, to collectively represent the length and sequencediversity of the human antibody CDRL3 repertoire. “Preimmune” versionsof these libraries have similar sequence diversities and lengthdiversities as naturally occurring human antibody CDRL3 sequences beforethese sequences undergo negative selection and/or somatic hypermutation.Known human CDRL3 sequences are represented in various data sets,including the NCBI database, WO/2009/036379, and Martin, Proteins, 1996,25: 130 each incorporated by reference in its entirety.

General Design of Libraries

Antibody libraries provided by the present invention may be designed toreflect certain aspects of the preimmune repertoire as created by thehuman immune system. Certain libraries of the invention are based onrational design informed by collections of human V, D, and J genes, andlarge databases of human heavy and light chain sequences (e.g., publiclyknown germline sequences and sequences from Jackson et al., J. Immunol.Methods, 2007, 324: 26; Lee et al., Immunogenetics, 2006, 57: 917; Boydet al., Science Translational Medicine, 2009, 1: 1-8, each incorporatedby reference in its entirety; and sequences compiled from rearranged VKand Vλ sequences (see WO/2009/036379, also incorporated by reference inits entirety). Additional information may be found, for example, inScaviner et al., Exp. Clin. Immunogenet., 1999, 16: 234; Tomlinson etal., J. Mol. Biol., 1992, 227: 799; and Matsuda et al., J. Exp. Med.,1998, 188: 2151, each incorporated by reference in its entirety.

In certain embodiments of the invention, segments representing thepossible V, D, and J diversity found in the human repertoire, as well asjunctional diversity (i.e., TN1 and N2), are synthesized de novo assingle or double-stranded DNA oligonucleotides. In certain embodimentsof the invention, oligonucleotides encoding CDR sequences are introducedinto yeast along with one or more acceptor vectors containing heavy orlight chain chassis sequences and constant domains. No primer-based PCRamplification or template-directed cloning steps from mammalian cDNA ormRNA are employed. Through standard homologous recombination, therecipient yeast recombines the CDR segments with the acceptor vectorscontaining the chassis sequences and constant regions, to create aproperly ordered synthetic, full-length human heavy chain and/or lightchain immunoglobulin library that can be genetically propagated,expressed, presented, and screened. One of ordinary skill in the artwill readily recognize that the acceptor vector can be designed so as toproduce constructs other than full-length human heavy chains and/orlight chains. For example, in certain embodiments of the invention, thechassis may be designed to encode portions of a polypeptide encoding anantibody fragment or subunit of an antibody fragment, so that a sequenceencoding an antibody fragment, or subunit thereof, is produced when theoligonucleotide cassette containing the CDR is recombined with theacceptor vector.

Thus, in certain embodiments, the invention provides a synthetic,preimmune human antibody repertoire the repertoire comprising:

(a) one or more selected human antibody heavy chain chassis (i.e., aminoacids 1 to 94 of the heavy chain variable region, using Kabat'sdefinition);

(b) a CDRH3 repertoire (described more fully below), designed based onthe human IGHD and IGHJ germline sequences, and the extraction of TN1and N2 sequences from reference sets of human CDRH3 sequences, the CDRH3repertoire comprising (i) a TN1 segment; (ii) a DH segment; (iii) an N2segment; (iv) an H3-JH segment.

(c) one or more selected human antibody kappa and/or lambda light chainchassis; and

(d) a CDRL3 repertoire designed based on the human IGLV and IGLJgermline sequences, wherein “L” may be a kappa or lambda light chain.

The instant invention also provides methods for producing and using suchlibraries, as well as libraries comprising one or more immunoglobulindomains or antibody fragments. Design and synthesis of each component ofthe antibody libraries of the invention is provided in more detailbelow.

Design of Antibody Library Chassis Sequences

In certain embodiments, provided libraries are constructed from selectedchassis sequences that are based on naturally occurring variable domainsequences (e.g., IGHV and IGLV genes). The selection of such chassissequences can be done arbitrarily, or through the definition of certainpre-determined criteria. For example, the Kabat database, an electronicdatabase containing non-redundant rearranged antibody sequences, can bequeried for those heavy and light chain germline sequences that are mostfrequently represented. An algorithm such as BLAST, or a morespecialized tool such as SoDA (Volpe et al., Bioinformatics, 2006, 22:438-44, incorporated by reference in its entirety), can be used tocompare rearranged antibody sequences with germline sequences (e.g.,using the V BASE2 database; see, for example, Retter et al., NucleicAcids Res., 2005, 33: D671-D674, incorporated by reference in itsentirety), or similar collections of human V, D, and J genes, toidentify germline families that are most frequently used to generatefunctional antibodies.

Several criteria can be utilized for the selection of chassis forinclusion in the libraries of the invention. For example, sequences thatare known (or have been determined) to express poorly in yeast, or otherorganisms used in the invention (e.g., bacteria, mammalian cells, fungi,or plants) can be excluded from the libraries. Chassis may also bechosen based on the representation of their corresponding germline genesin the peripheral blood of humans. In certain embodiments of theinvention, it may be desirable to select chassis that correspond togermline sequences that are highly represented in the peripheral bloodof humans. In some embodiments, it may be desirable to select chassisthat correspond to germline sequences that are less frequentlyrepresented, for example, to increase the canonical diversity of thelibrary. Therefore, chassis may be selected to produce libraries thatrepresent the largest and most structurally diverse group of functionalhuman antibodies.

In certain embodiments of the invention, less diverse chassis may beutilized, for example, if it is desirable to produce a smaller, morefocused library with less chassis variability and greater CDRvariability. In some embodiments of the invention, chassis may beselected based on both their expression in a cell of the invention(e.g., a yeast cell) and the diversity of canonical structuresrepresented by the selected sequences. One may therefore produce alibrary with a diversity of canonical structures that express well in acell of the invention.

Design of Heavy Chain Chassis Sequences

The design and selection of heavy chain chassis sequences that can beused in the current invention is described in detail in U.S. PublicationNos. 2009/0181855 and 2010/0056386, and WO/2009/036379, each of which isincorporated by reference in its entirety, and is therefore describedonly briefly here.

In general, VH domains of the library comprise three components: (1) aVH “chassis,” which includes amino acids 1 to 94 (using Kabatnumbering), (2) the CDRH3, which is defined herein to include the KabatCDRH3 proper (positions 95-102), and (3) the FRM4 region, includingamino acids 103 to 113 (Kabat numbering). The overall VH domainstructure may therefore be depicted schematically (not to scale) as:

In certain embodiments of the invention, the VH chassis of the librariesmay comprise from about Kabat residue 1 to about Kabat residue 94 of oneor more of the following IGHV germline sequences: IGHV1-2, IGHV1-3,IGHV1-8, IGHV1-18, IGHV1-24, IGHV1-45, IGHV1-46, IGHV1-58, IGHV1-69,IGH8, IGH56, IGH100, IGHV3-7, IGHV3-9, IGHV3-11, IGHV3-13, IGHV3-15,IGHV3-20, IGHV3-21, IGHV3-23, IGHV3-30, IGHV3-33, IGHV3-43, IGHV3-48,IGHV3-49, IGHV3-53, IGHV3-64, IGHV3-66, IGHV3-72, IGHV3-73, IGHV3-74,IGHV4-4, IGHV4-28, IGHV4-31, IGHV4-34, IGHV4-39, IGHV4-59, IGHV4-61,IGHV4-B, IGHV5-51, IGHV6-1, and/or IGHV7-4-1. In some embodiments of theinvention, a library may contain one or more of these sequences, one ormore allelic variants of these sequences, or encode an amino acidsequence at least about 99.9%, 99.5%, 99%, 98.5%, 98%, 97.5%, 97%,96.5%, 96%, 95.5%, 95%, 94.5%, 94%, 93.5%, 93%, 92.5%, 92%, 91.5%, 91%,90.5%, 90%, 89%, 88%, 87%, 86%, 85%, 84%, 83%, 82%, 81%, 80%, 77.5%,75%, 73.5%, 70%, 65%, 60%, 55%, or 50% identical to one or more of thesesequences. One of ordinary skill in the art will recognize that giventhe chassis definition provided above, any IGHV-encoding sequence can beadapted for use as a chassis of the invention. As exemplified in U.S.Publication Nos. 2009/0181855 and 2010/0056386, and WO/2009/036379 (eachincorporated by reference in its entirety), these chassis can also bevaried, particularly by altering the amino acid residues in the CDRH1and CDRH2 regions, further increasing the diversity of the library.

Design of Light Chain Chassis Sequences

The design and selection of light chain chassis sequences that can beused in the current invention is described in detail in U.S. PublicationNos. 2009/0181855 and 2010/0056386, and WO/2009/036379, each of which isincorporated by reference in its entirety, and is therefore describedonly briefly here. The light chain chassis of the invention may be basedon kappa and/or lambda light chain sequences.

The VL domains of the library comprise three primary components: (1) aVL “chassis”, which includes amino acids 1 to 88 (using Kabatnumbering), (2) the CDRL3, which is defined herein to include the KabatCDRL3 proper (positions 89-97), and (3) the FRM4 region, including aminoacids 98 to 107 (Kabat numbering). The overall VL domain structure maytherefore be depicted schematically (not to scale) as:

In certain embodiments of the invention, the VL chassis of the librariesinclude one or more chassis based on IGKV germline sequences. In certainembodiments of the invention, the VL chassis of the libraries maycomprise from about Kabat residue 1 to about Kabat residue 88 of one ormore of the following IGKV germline sequences: IGKV1-05, IGKV1-06,IGKV1-08, IGKV1-09, IGKV1-12, IGKV1-13, IGKV1-16, IGKV1-17, IGKV1-27,IGKV1-33, IGKV1-37, IGKV1-39, IGKV1D-16, IGKV1D-17, IGKV1D-43, IGKV1D-8,IGK54, IGK58, IGK59, IGK60, IGK70, IGKV2D-26, IGKV2D-29, IGKV2D-30,IGKV3-11, IGKV3-15, IGKV3-20, IGKV3D-07, IGKV3D-11, IGKV3D-20, IGKV4-1,IGKV5-2, IGKV6-21, and/or IGKV6D-41. In some embodiments of theinvention, a library may contain one or more of these sequences, one ormore allelic variants of these sequences, or encode an amino acidsequence at least about 99.9%, 99.5%, 99%, 98.5%, 98%, 97.5%, 97%,96.5%, 96%, 95.5%, 95%, 94.5%, 94%, 93.5%, 93%, 92.5%, 92%, 91.5%, 91%,90.5%, 90%, 89%, 88%, 87%, 86%, 85%, 84%, 83%, 82%, 81%, 80%, 77.5%,75%, 73.5%, 70%, 65%, 60%, 55%, or 50% identical to one or more of thesesequences.

In certain embodiments of the invention, the VL chassis of the librariesinclude one or more chassis based on IGλV germline sequences. In certainembodiments of the invention, the VL chassis of the libraries maycomprise from about Kabat residue 1 to about Kabat residue 88 of one ormore of the following IGλV germline sequences: IGλV3-1, IGλV3-21, IGλ44,IGλV1-40, IGλV3-19, IGλV1-51, IGλV1-44, IGλV6-57, IGλ11, IGλV3-25,IGλ53, IGλV3-10, IGλV4-69, IGλV1-47, IGλ41, IGλV7-43, IGλV7-46,IGλV5-45, IGλV4-60, IGλV10-54, IGλV8-61, IGλV3-9, IGλV1-36, IGλ48,IGλV3-16, IGλV3-27, IGλV4-3, IGλV5-39, IGλV9-49, and/or IGλV3-12. Insome embodiments of the invention, a library may contain one or more ofthese sequences, one or more allelic variants of these sequences, orencode an amino acid sequence at least about 99.9%, 99.5%, 99%, 98%,97%, 96%, 95%, 94%, 93%, 92%, 91%, 90%, 85%, 80%, 75%, 70%, 65%, 60%,55%, or 50% identical to one or more of these sequences.

One of ordinary skill in the art will recognize that given the chassisdefinition provided above, any IGKV- or IGλV-encoding sequence can beadapted for use as a chassis of the invention.

Design and Selection of TN1, DH, N2, and H3-JH Segments

The human germline repertoire contains at least six IGHJ genes (IGHJ1,IGHJ2, IGHJ3, IGHJ4, IGHJ5, and IGHJ6; included in Table 14, where theprimary allele is designated “01,” and selected allelic variants aredesignated “02” or “03”), and at least 27 IGHD genes (Table 16,including allelic variants). In some embodiments, the inventioncomprises a library of CDRH3 polypeptide sequences, or polynucleotidesequences encoding CDRH3 sequences, the library comprising members ofany of the theoretical segment pools disclosed herein.

A person of ordinary skill in the art will recognize that not everysegment in a theoretical segment pool provided herein is necessary toproduce a functional CDRH3 library of the invention. Therefore, incertain embodiments, a CDRH3 library of the invention will contain asubset of the segments of any of the theoretical segment pools describedherein. For example, in certain embodiments of the invention, at leastabout 15, 30, 45, 60, 75, 90, 100, 105, 120, 135, 150, 165, 180, 195,200, 210, 225, 240, 255, 270, 285, 300, 320, 340, 360, 380, 400, 420,440, 460, 480, 500, 520, 540, 560, 580, 600, 620, 640, or 643 of theH3-JH segments of any of the theoretical segment pools provided herein,or generated by the methods described herein, are included in a library.In some embodiments of the invention, at least about 15, 30, 45, 60, 75,90, 100, 105, 120, 135, 150, 165, 180, 195, 200, 250, 300, 350, 400,450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100,1111, 2000, 3000, 4000, 5000, 6000, 7000, 14000, 21000, 28000, 35000,42000, 49000, 56000, 63000, or 68374 of the DH segments of any of thetheoretical segment pools provided herein, or generated by the methodsdescribed herein, are included in a library. In some embodiments of theinvention, at least about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110,120, 130, 140, 141, 150, 160, 170, 180, 190, or 200, 220, 240, 260, 280,300, 320, 340, 360, 380, 400, 420, 424, 440, 460, 480, 500, 550, 600,650, 700, 727, 750, 800, 850, 900, 950, or 1000 of the TN1 and/or N2segments of any of the theoretical segment pools provided herein, orgenerated by the methods described herein, are included in a library. Incertain embodiments, a library of the invention may contain less than aparticular number of polynucleotide or polypeptide segments, where thenumber of segments is defined using any one of the integers providedabove for the respective segment. In some embodiments of the invention,a particular numerical range is defined, using any two of the integersprovided above as lower and upper boundaries of the range, inclusive orexclusive. All combinations of the integers provided, which define anupper and lower boundary, are contemplated.

In certain embodiments, the invention provides CDRH3 librariescomprising at least about 1%, 2.5%, 5%, 10%, 15%, 20%, 25%, 30%, 40%,45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% of thesegments from any of the theoretical segment pools provided herein. Forexample, the invention provides libraries comprising at least about 1%,2.5%, 5%, 10%, 15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%,75%, 80%, 85%, 90%, 95%, or 99% of the TN1, DH, N2, and/or H3-JHsegments from any of the theoretical segment pools provided herein. Insome embodiments of the invention, a particular percentage range isdefined, using any two of the percentages provided above as lower andupper boundaries of the range, inclusive or exclusive. All combinationsof the percentages provided, which define an upper and lower boundary,are contemplated.

In some embodiments of the invention, at least about 1%, 2.5%, 5%, 10%,15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%,90%, 95%, or 99% of the H3-JH, DH, TN1, and/or N2 segments in a CDRH3library are H3-JH, DH, TN1, and/or N2 segments of any of the theoreticalsegment pools provided herein, or generated by the methods describedherein. In some embodiments of the invention, at least about 1%, 2.5%,5%, 10%, 15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%,80%, 85%, 90%, 95%, or 99% of the H3-JH, DH, TN1, and/or N2 segments ofantibodies isolated from a CDRH3 library (e.g., by binding to aparticular antigen and/or generic ligand through one or more rounds ofselection) are H3-JH, DH, TN1, and/or N2 segments of any of thetheoretical segment pools provided herein, or generated by methodsdescribed herein. In certain embodiments, a CDRH3 library of theinvention may contain less than a particular percentage of H3-JH, DH,TN1, and/or N2 segments of any of the theoretical segment pools providedherein, or generated by the methods described herein, where the numberof segments is defined using any one of the percentages provided abovefor the respective segment. In some embodiments of the invention, aparticular percentage range is defined, using any two of the percentagesprovided above as lower and upper boundaries of the range, inclusive orexclusive. All combinations of the percentages provided, which define anupper and lower boundary, are contemplated.

One of ordinary skill in the art will appreciate, upon reading thedisclosure herein. that given the TN1, DH, N2, and/or H3-JH segments ofany of the theoretical segment pools provided herein, or generated bythe methods described herein, similar TN1, DH, N2, and/or H3-JHsegments, and corresponding CDRH3 libraries, could be produced which,while not 100% identical to those provided in terms of their sequences,may be functionally very similar. Such theoretical segment pools andCDRH3 libraries also fall within the scope of the invention. A varietyof techniques well-known in the art could be used to obtain theseadditional sequences, including the mutagenesis techniques providedherein. Therefore, each of the explicitly enumerated embodiments of theinvention can also be practiced using segments that share a particularpercent identity to any of the segments of any of the theoreticalsegment pools provided herein, or generated by the methods describedherein. For example, each of the previously described embodiments of theinvention can be practiced using TN1, DH, N2, and/or H3-JH segments thatare at least about 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%,92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 99.9% identical to theTN1, DH, N2, and/or H3-JH segments of any of the theoretical segmentpools provided herein, or generated by the methods described herein.

In some embodiments, the invention provides libraries produced from oneor more VH chassis sequences combined with one or more TN1 segments, oneor more DH segments, one or more N2 segments, and one or more H3-JHsegments. In certain embodiments at least 1, 2, 5, 10, 20, 50, 75, or100, of each chassis, TN1, DH, N2, or H3-JH segments are included in alibrary of the invention.

In some embodiments, the invention provides a method of selecting TN1,DH, N2, and H3-JH segments from a theoretical segment pool for inclusionin a synthetic CDRH3 library, comprising:

-   -   (i) providing a theoretical segment pool containing one or more        of TN1, DH, N2, and H3-JH segments;    -   (ii) providing a reference set of CDRH3 sequences;    -   (iii) utilizing the theoretical segment pool of (i) to identify        the closest match(es) to each CDRH3 sequence in the reference        set of (ii); and    -   (iv) selecting segments from the theoretical segment pool for        inclusion in a synthetic library.

In some embodiments, the selection process of (iv) can optionallyinvolve any number of additional criteria, including the frequency ofoccurrence of the segments of (i) in the reference set of (ii); thecorresponding segmental usage weights; and any physicochemicalproperties (see all numerical indices at www.genome.jp/aaindex/) of thesegments (e.g., hydrophobicity, alpha-helical propensity, and/orisoelectric point). Optionally, TN1 and/or N2 segments that do not occurin the theoretical segment pool of (i) but that are found in thereference set of (ii) may be identified and added to prospectivetheoretical segment pools to produce theoretical segment pools withincreased TN1 and/or N2 diversity in the prospective theoretical segmentpools and/or synthetic libraries of the invention.

Any characteristic or set of characteristics of the segments can be usedto choose them for inclusion in the library, including for example oneor more biological properties (e.g., immunogenicity, stability,half-life) and/or one or more physicochemical properties such as thenumerical indices provided above. In some embodiments, at least 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more such properties is usedto select segments for inclusion in a library of the invention.

Physiochemical properties included in the index provided above caninclude, for example, ANDN920101 alpha-CH chemical shifts (Andersen etal., 1992); ARGP820101 Hydrophobicity index (Argos et al., 1982);ARGP820102 Signal sequence helical potential (Argos et al., 1982);ARGP820103 Membrane-buried preference parameters (Argos et al., 1982);BEGF750101 Conformational parameter of inner helix (Beghin-Dirkx, 1975);BEGF750102 Conformational parameter of beta-structure (Beghin-Dirkx,1975); BEGF750103 Conformational parameter of beta-turn (Beghin-Dirkx,1975); BHAR880101Average flexibility indices (Bhaskaran-Ponnuswamy,1988); BIGC670101 Residue volume (Bigelow, 1967); BIOV880101 Informationvalue for accessibility; average fraction 35% (Biou et al., 1988);BIOV880102 Information value for accessibility; average fraction 23%(Biou et al., 1988); BROC820101 Retention coefficient in TFA (Browne etal., 1982); BROC820102 Retention coefficient in HFBA (Browne et al.,1982); BULH740101 Transfer free energy to surface (Bull-Breese, 1974);BULH740102 Apparent partial specific volume (Bull-Breese, 1974);BUNA790101 alpha-NH chemical shifts (Bundi-Wuthrich, 1979); BUNA790102alpha-CH chemical shifts (Bundi-Wuthrich, 1979); BUNA790103 Spin-spincoupling constants 3JHalpha-NH (Bundi-Wuthrich, 1979); BURA740101Normalized frequency of alpha-helix (Burgess et al., 1974); BURA740102Normalized frequency of extended structure (Burgess et al., 1974);CHAM810101 Steric parameter (Charton, 1981); CHAM820101 Polarizabilityparameter (Charton-Charton, 1982); CHAM820102 Free energy of solution inwater, kcal/mole (Charton-Charton, 1982); CHAM830101 The Chou-Fasmanparameter of the coil conformation (Charton-Charton, 1983); CHAM830102 Aparameter defined from the residuals obtained from the best correlationof the Chou-Fasman parameter of beta-sheet (Charton-Charton, 1983);CHAM830103 The number of atoms in the side chain labelled 1+1(Charton-Charton, 1983); CHAM830104 The number of atoms in the sidechain labelled 2+1 (Charton-Charton, 1983); CHAM830105 The number ofatoms in the side chain labelled 3+1 (Charton-Charton, 1983); CHAM830106The number of bonds in the longest chain (Charton-Charton, 1983);CHAM830107 A parameter of charge transfer capability (Charton-Charton,1983); CHAM830108 A parameter of charge transfer donor capability(Charton-Charton, 1983); CHOC750101 Average volume of buried residue(Chothia, 1975); CHOC760101 Residue accessible surface area intripeptide (Chothia, 1976); CHOC760102 Residue accessible surface areain folded protein (Chothia, 1976); CHOC760103 Proportion of residues 95%buried (Chothia, 1976); CHOC760104 Proportion of residues 100% buried(Chothia, 1976); CHOP780101 Normalized frequency of beta-turn(Chou-Fasman, 1978a); CHOP780201 Normalized frequency of alpha-helix(Chou-Fasman, 1978b); CHOP780202 Normalized frequency of beta-sheet(Chou-Fasman, 1978b); CHOP780203 Normalized frequency of beta-turn(Chou-Fasman, 1978b); CHOP780204 Normalized frequency of N-terminalhelix (Chou-Fasman, 1978b); CHOP780205 Normalized frequency ofC-terminal helix (Chou-Fasman, 1978b); CHOP780206 Normalized frequencyof N-terminal non helical region (Chou-Fasman, 1978b); CHOP780207Normalized frequency of C-terminal non helical region (Chou-Fasman,1978b); CHOP780208 Normalized frequency of N-terminal beta-sheet(Chou-Fasman, 1978b); CHOP780209 Normalized frequency of C-terminalbeta-sheet (Chou-Fasman, 1978b); CHOP780210 Normalized frequency ofN-terminal non beta region (Chou-Fasman, 1978b); CHOP780211 Normalizedfrequency of C-terminal non beta region (Chou-Fasman, 1978b); CHOP780212Frequency of the 1st residue in turn (Chou-Fasman, 1978b); CHOP780213Frequency of the 2nd residue in turn (Chou-Fasman, 1978b); CHOP780214Frequency of the 3rd residue in turn (Chou-Fasman, 1978b); CHOP780215Frequency of the 4th residue in turn (Chou-Fasman, 1978b); CHOP780216Normalized frequency of the 2nd and 3rd residues in turn (Chou-Fasman,1978b); CIDH920101 Normalized hydrophobicity scales for alpha-proteins(Cid et al., 1992); CIDH920102 Normalized hydrophobicity scales forbeta-proteins (Cid et al., 1992); CIDH920103 Normalized hydrophobicityscales for alpha+beta-proteins (Cid et al., 1992); CIDH920104 Normalizedhydrophobicity scales for alpha/beta-proteins (Cid et al., 1992);CIDH920105 Normalized average hydrophobicity scales (Cid et al., 1992);COHE430101 Partial specific volume (Cohn-Edsall, 1943); CRAJ730101Normalized frequency of middle helix (Crawford et al., 1973); CRAJ730102Normalized frequency of beta-sheet (Crawford et al., 1973); CRAJ730103Normalized frequency of turn (Crawford et al., 1973); DAWD720101 Size(Dawson, 1972); DAYM780101 Amino acid composition (Dayhoff et al.,1978a); DAYM780201 Relative mutability (Dayhoff et al., 1978b);DESM900101 Membrane preference for cytochrome b: MPH89 (Degli Esposti etal., 1990); DESM900102 Average membrane preference: AMP07 (Degli Espostiet al., 1990); EISD840101 Consensus normalized hydrophobicity scale(Eisenberg, 1984); EISD860101 Solvation free energy(Eisenberg-McLachlan, 1986); EISD860102 Atom-based hydrophobic moment(Eisenberg-McLachlan, 1986); EISD860103 Direction of hydrophobic moment(Eisenberg-McLachlan, 1986); FASG760101 Molecular weight (Fasman, 1976);FASG760102 Melting point (Fasman, 1976); FASG760103 Optical rotation(Fasman, 1976); FASG760104 pK-N (Fasman, 1976); FASG760105 pK-C (Fasman,1976); FAUJ830101 Hydrophobic parameter pi (Fauchere-Pliska, 1983);FAUJ880101 Graph shape index (Fauchere et al., 1988); FAUJ880102Smoothed upsilon steric parameter (Fauchere et al., 1988); FAUJ880103Normalized van der Waals volume (Fauchere et al., 1988); FAUJ880104STERIMOL length of the side chain (Fauchere et al., 1988); FAUJ880105STERIMOL minimum width of the side chain (Fauchere et al., 1988);FAUJ880106 STERIMOL maximum width of the side chain (Fauchere et al.,1988); FAUJ880107 N.m.r. chemical shift of alpha-carbon (Fauchere etal., 1988); FAUJ880108 Localized electrical effect (Fauchere et al.,1988); FAUJ880109 Number of hydrogen bond donors (Fauchere et al.,1988); FAUJ880110 Number of full nonbonding orbitals (Fauchere et al.,1988); FAUJ880111 Positive charge (Fauchere et al., 1988); FAUJ880112Negative charge (Fauchere et al., 1988); FAUJ880113 pK-a(RCOOH)(Fauchere et al., 1988); FINA770101 Helix-coil equilibrium constant(Finkelstein-Ptitsyn, 1977); FINA910101 Helix initiation parameter atposision i−1 (Finkelstein et al., 1991); FINA910102 Helix initiationparameter at posision i, i+1, i+2 (Finkelstein et al., 1991); FINA910103Helix termination parameter at posision j−2, j−1,j (Finkelstein et al.,1991); FINA910104 Helix termination parameter at posision j+1(Finkelstein et al., 1991); GARJ730101 Partition coefficient (Garel etal., 1973); GEIM800101 Alpha-helix indices (Geisow-Roberts, 1980);GEIM800102 Alpha-helix indices for alpha-proteins (Geisow-Roberts,1980); GEIM800103 Alpha-helix indices for beta-proteins (Geisow-Roberts,1980); GEIM800104 Alpha-helix indices for alpha/beta-proteins(Geisow-Roberts, 1980); GEIM800105 Beta-strand indices (Geisow-Roberts,1980); GEIM800106 Beta-strand indices for beta-proteins (Geisow-Roberts,1980); GEIM800107 Beta-strand indices for alpha/beta-proteins(Geisow-Roberts, 1980) GEIM800108 Aperiodic indices (Geisow-Roberts,1980); GEIM800109 Aperiodic indices for alpha-proteins (Geisow-Roberts,1980); GEIM800110 Aperiodic indices for beta-proteins (Geisow-Roberts,1980); GEIM800111 Aperiodic indices for alpha/beta-proteins(Geisow-Roberts, 1980); GOLD730101 Hydrophobicity factor(Goldsack-Chalifoux, 1973); GOLD730102 Residue volume(Goldsack-Chalifoux, 1973); GRAR740101 Composition (Grantham, 1974);GRAR740102Polarity (Grantham, 1974) GRAR740103 Volume (Grantham, 1974);GUYH850101 Partition energy (Guy, 1985); HOPA770101 Hydration number(Hopfinger, 1971), Cited by Charton-Charton (1982) HOPT810101Hydrophilicity value (Hopp-Woods, 1981); HUTJ700101 Heat capacity(Hutchens, 1970); HUTJ700102 Absolute entropy (Hutchens, 1970);HUTJ700103 Entropy of formation (Hutchens, 1970); ISOY800101 Normalizedrelative frequency of alpha-helix (Isogai et al., 1980); ISOY800102Normalized relative frequency of extended structure (Isogai et al.,1980); ISOY800103 Normalized relative frequency of bend (Isogai et al.,1980); ISOY800104 Normalized relative frequency of bend R (Isogai etal., 1980); ISOY800105 Normalized relative frequency of bend S (Isogaiet al., 1980); ISOY800106 Normalized relative frequency of helix end(Isogai et al., 1980); ISOY800107 Normalized relative frequency ofdouble bend (Isogai et al., 1980); ISOY800108 Normalized relativefrequency of coil (Isogai et al., 1980); JANJ780101 Average accessiblesurface area (Janin et al., 1978); JANJ780102 Percentage of buriedresidues (Janin et al., 1978); JANJ780103 Percentage of exposed residues(Janin et al., 1978); JANJ790101 Ratio of buried and accessible molarfractions (Janin, 1979); JANJ790102 Transfer free energy (Janin, 1979);JOND750101 Hydrophobicity (Jones, 1975); JOND750102 pK (—COOH) (Jones,1975); JOND920101 Relative frequency of occurrence (Jones et al., 1992);JOND920102 Relative mutability (Jones et al., 1992) JUKT750101 Aminoacid distribution (Jukes et al., 1975); JUNJ780101 Sequence frequency(Jungck, 1978); KANM800101 Average relative probability of helix(Kanehisa-Tsong, 1980); KANM800102 Average relative probability ofbeta-sheet (Kanehisa-Tsong, 1980); KANM800103 Average relativeprobability of inner helix (Kanehisa-Tsong, 1980); KANM800104 Averagerelative probability of inner beta-sheet (Kanehisa-Tsong, 1980);KARP850101 Flexibility parameter for no rigid neighbors (Karplus-Schulz,1985); KARP850102 Flexibility parameter for one rigid neighbor(Karplus-Schulz, 1985); KARP850103 Flexibility parameter for two rigidneighbors (Karplus-Schulz, 1985); KHAG800101 The Kerr-constantincrements (Khanarian-Moore, 1980); KLEP840101 Net charge (Klein et al.,1984); KRIW710101 Side chain interaction parameter (Krigbaum-Rubin,1971); KRIW790101 Side chain interaction parameter (Krigbaum-Komoriya,1979); KRIW790102 Fraction of site occupied by water (Krigbaum-Komoriya,1979); KRIW790103 Side chain volume (Krigbaum-Komoriya, 1979);KYTJ820101 Hydropathy index (Kyte-Doolittle, 1982); LAWE840101 Transferfree energy, CHP/water (Lawson et al., 1984); LEVM760101 Hydrophobicparameter (Levitt, 1976); LEVM760102 Distance between C-alpha andcentroid of side chain (Levitt, 1976); LEVM760103 Side chain angletheta(AAR) (Levitt, 1976); LEVM760104 Side chain torsion angle phi(AAAR)(Levitt, 1976); LEVM760105 Radius of gyration of side chain (Levitt,1976); LEVM760106 van der Waals parameter R0 (Levitt, 1976) LEVM760107van der Waals parameter epsilon (Levitt, 1976); LEVM780101 Normalizedfrequency of alpha-helix, with weights (Levitt, 1978); LEVM780102Normalized frequency of beta-sheet, with weights (Levitt, 1978);LEVM780103 Normalized frequency of reverse turn, with weights (Levitt,1978); LEVM780104 Normalized frequency of alpha-helix, unweighted(Levitt, 1978); LEVM780105 Normalized frequency of beta-sheet,unweighted (Levitt, 1978); LEVM780106 Normalized frequency of reverseturn, unweighted (Levitt, 1978); LEWP710101 Frequency of occurrence inbeta-bends (Lewis et al., 1971); LIFS790101 Conformational preferencefor all beta-strands (Lifson-Sander, 1979); LIFS790102 Conformationalpreference for parallel beta-strands (Lifson-Sander, 1979); LIFS790103Conformational preference for antiparallel beta-strands (Lifson-Sander,1979); MANP780101 Average surrounding hydrophobicity(Manavalan-Ponnuswamy, 1978); MAXF760101 Normalized frequency ofalpha-helix (Maxfield-Scheraga, 1976); MAXF760102 Normalized frequencyof extended structure (Maxfield-Scheraga, 1976); MAXF760103 Normalizedfrequency of zeta R (Maxfield-Scheraga, 1976); MAXF760104 Normalizedfrequency of left-handed alpha-helix (Maxfield-Scheraga, 1976);MAXF760105 Normalized frequency of zeta L (Maxfield-Scheraga, 1976);MAXF760106 Normalized frequency of alpha region (Maxfield-Scheraga,1976); MCMT640101 Refractivity (McMeekin et al., 1964), Cited by Jones(1975); MEEJ800101 Retention coefficient in HPLC, pH7.4 (Meek, 1980);MEEJ800102 Retention coefficient in HPLC, pH2.1 (Meek, 1980); MEEJ810101Retention coefficient in NaClO4 (Meek-Rossetti, 1981); MEEJ810102Retention coefficient in NaH2PO4 (Meek-Rossetti, 1981); MEIH800101Average reduced distance for C-alpha (Meirovitch et al., 1980);MEIH800102 Average reduced distance for side chain (Meirovitch et al.,1980); MEIH800103 Average side chain orientation angle (Meirovitch etal., 1980); MIYS850101 Effective partition energy (Miyazawa-Jernigan,1985); NAGK730101 Normalized frequency of alpha-helix (Nagano, 1973);NAGK730102 Normalized frequency of bata-structure (Nagano, 1973)NAGK730103 Normalized frequency of coil (Nagano, 1973); NAKH900101 AAcomposition of total proteins (Nakashima et al., 1990); NAKH900102 SD ofAA composition of total proteins (Nakashima et al., 1990); NAKH900103 AAcomposition of mt-proteins (Nakashima et al., 1990); NAKH900104Normalized composition of mt-proteins (Nakashima et al., 1990);NAKH900105 AA composition of mt-proteins from animal (Nakashima et al.,1990); NAKH900106 Normalized composition from animal (Nakashima et al.,1990); NAKH900107 AA composition of mt-proteins from fungi and plant(Nakashima et al., 1990); NAKH900108 Normalized composition from fungiand plant (Nakashima et al., 1990); NAKH900109 AA composition ofmembrane proteins (Nakashima et al., 1990); NAKH900110 Normalizedcomposition of membrane proteins (Nakashima et al., 1990); NAKH900111Transmembrane regions of non-mt-proteins (Nakashima et al., 1990);NAKH900112 Transmembrane regions of mt-proteins (Nakashima et al.,1990); NAKH900113 Ratio of average and computed composition (Nakashimaet al., 1990); NAKH920101 AA composition of CYT of single-spanningproteins (Nakashima-Nishikawa, 1992); NAKH920102 AA composition of CYT2of single-spanning proteins (Nakashima-Nishikawa, 1992); NAKH920103 AAcomposition of EXT of single-spanning proteins (Nakashima-Nishikawa,1992); NAKH920104 AA composition of EXT2 of single-spanning proteins(Nakashima-Nishikawa, 1992); NAKH920105 AA composition of MEM ofsingle-spanning proteins (Nakashima-Nishikawa, 1992); NAKH920106 AAcomposition of CYT of multi-spanning proteins (Nakashima-Nishikawa,1992); NAKH920107 AA composition of EXT of multi-spanning proteins(Nakashima-Nishikawa, 1992); NAKH920108 AA composition of MEM ofmulti-spanning proteins (Nakashima-Nishikawa, 1992); NISK800101 8 Acontact number (Nishikawa-Ooi, 1980); NISK860101 14 A contact number(Nishikawa-Ooi, 1986); NOZY710101 Transfer energy, organic solvent/water(Nozaki-Tanford, 1971); OOBM770101 Average non-bonded energy per atom(Oobatake-Ooi, 1977); OOBM770102 Short and medium range non-bondedenergy per atom (Oobatake-Ooi, 1977); OOBM770103 Long range non-bondedenergy per atom (Oobatake-Ooi, 1977) OOBM770104 Average non-bondedenergy per residue (Oobatake-Ooi, 1977); OOBM770105 Short and mediumrange non-bonded energy per residue (Oobatake-Ooi, 1977); OOBM850101Optimized beta-structure-coil equilibrium constant (Oobatake et al.,1985); OOBM850102 Optimized propensity to form reverse turn (Oobatake etal., 1985); OOBM850103 Optimized transfer energy parameter (Oobatake etal., 1985); OOBM850104 Optimized average non-bonded energy per atom(Oobatake et al., 1985); OOBM850105 Optimized side chain interactionparameter (Oobatake et al., 1985); PALJ810101 Normalized frequency ofalpha-helix from LG (Palau et al., 1981); PALJ810102 Normalizedfrequency of alpha-helix from CF (Palau et al., 1981); PALJ810103Normalized frequency of beta-sheet from LG (Palau et al., 1981);PALJ810104 Normalized frequency of beta-sheet from CF (Palau et al.,1981); PALJ810105 Normalized frequency of turn from LG (Palau et al.,1981); PALJ810106 Normalized frequency of turn from CF (Palau et al.,1981); PALJ810107 Normalized frequency of alpha-helix in all-alpha class(Palau et al., 1981); PALJ810108 Normalized frequency of alpha-helix inalpha+beta class (Palau et al., 1981); PALJ810109 Normalized frequencyof alpha-helix in alpha/beta class (Palau et al., 1981); PALJ810110Normalized frequency of beta-sheet in all-beta class (Palau et al.,1981); PALJ810111 Normalized frequency of beta-sheet in alpha+beta class(Palau et al., 1981); PALJ810112 Normalized frequency of beta-sheet inalpha/beta class (Palau et al., 1981); PALJ810113 Normalized frequencyof turn in all-alpha class (Palau et al., 1981); PALJ810114 Normalizedfrequency of turn in all-beta class (Palau et al., 1981); PALJ810115Normalized frequency of turn in alpha+beta class (Palau et al., 1981);PALJ810116 Normalized frequency of turn in alpha/beta class (Palau etal., 1981); PARJ860101 HPLC parameter (Parker et al., 1986); PLIV810101Partition coefficient (Pliska et al., 1981); PONP800101 Surroundinghydrophobicity in folded form (Ponnuswamy et al., 1980); PONP800102Average gain in surrounding hydrophobicity (Ponnuswamy et al., 1980);PONP800103 Average gain ratio in surrounding hydrophobicity (Ponnuswamyet al., 1980); PONP800104 Surrounding hydrophobicity in alpha-helix(Ponnuswamy et al., 1980); PONP800105 Surrounding hydrophobicity inbeta-sheet (Ponnuswamy et al., 1980); PONP800106 Surroundinghydrophobicity in turn (Ponnuswamy et al., 1980); PONP800107Accessibility reduction ratio (Ponnuswamy et al., 1980); PONP800108Average number of surrounding residues (Ponnuswamy et al., 1980);PRAM820101 Intercept in regression analysis (Prabhakaran-Ponnuswamy,1982); PRAM820102 Slope in regression analysis ×1.0E1(Prabhakaran-Ponnuswamy, 1982); PRAM820103 Correlation coefficient inregression analysis (Prabhakaran-Ponnuswamy, 1982); PRAM900101Hydrophobicity (Prabhakaran, 1990); PRAM900102 Relative frequency inalpha-helix (Prabhakaran, 1990); PRAM900103 Relative frequency inbeta-sheet (Prabhakaran, 1990); PRAM900104 Relative frequency inreverse-turn (Prabhakaran, 1990); PTIO830101 Helix-coil equilibriumconstant (Ptitsyn-Finkelstein, 1983); PTIO830102 Beta-coil equilibriumconstant (Ptitsyn-Finkelstein, 1983); QIAN880101 Weights for alpha-helixat the window position of −6 (Qian-Sejnowski, 1988); QIAN880102 Weightsfor alpha-helix at the window position of −5 (Qian-Sejnowski, 1988);QIAN880103 Weights for alpha-helix at the window position of −4(Qian-Sejnowski, 1988); QIAN880104 Weights for alpha-helix at the windowposition of −3 (Qian-Sejnowski, 1988); QIAN880105 Weights foralpha-helix at the window position of −2 (Qian-Sejnowski, 1988);QIAN880106 Weights for alpha-helix at the window position of −1(Qian-Sejnowski, 1988); QIAN880107 Weights for alpha-helix at the windowposition of 0 (Qian-Sejnowski, 1988); QIAN880108 Weights for alpha-helixat the window position of 1 (Qian-Sejnowski, 1988); QIAN880109 Weightsfor alpha-helix at the window position of 2 (Qian-Sejnowski, 1988);QIAN880110 Weights for alpha-helix at the window position of 3(Qian-Sejnowski, 1988); QIAN880111 Weights for alpha-helix at the windowposition of 4 (Qian-Sejnowski, 1988); QIAN880112 Weights for alpha-helixat the window position of 5 (Qian-Sejnowski, 1988); QIAN880113 Weightsfor alpha-helix at the window position of 6 (Qian-Sejnowski, 1988);QIAN880114 Weights for beta-sheet at the window position of −6(Qian-Sejnowski, 1988); QIAN880115 Weights for beta-sheet at the windowposition of −5 (Qian-Sejnowski, 1988); QIAN880116 Weights for beta-sheetat the window position of −4 (Qian-Sejnowski, 1988); QIAN880117 Weightsfor beta-sheet at the window position of −3 (Qian-Sejnowski, 1988);QIAN880118 Weights for beta-sheet at the window position of −2(Qian-Sejnowski, 1988); QIAN880119 Weights for beta-sheet at the windowposition of −1 (Qian-Sejnowski, 1988); QIAN880120 Weights for beta-sheetat the window position of 0 (Qian-Sejnowski, 1988); QIAN880121 Weightsfor beta-sheet at the window position of 1 (Qian-Sejnowski, 1988);QIAN880122 Weights for beta-sheet at the window position of 2(Qian-Sejnowski, 1988); QIAN880123 Weights for beta-sheet at the windowposition of 3 (Qian-Sejnowski, 1988); QIAN880124 Weights for beta-sheetat the window position of 4 (Qian-Sejnowski, 1988); QIAN880125 Weightsfor beta-sheet at the window position of 5 (Qian-Sejnowski, 1988);QIAN880126 Weights for beta-sheet at the window position of 6(Qian-Sejnowski, 1988); QIAN880127 Weights for coil at the windowposition of −6 (Qian-Sejnowski, 1988); QIAN880128 Weights for coil atthe window position of −5 (Qian-Sejnowski, 1988); QIAN880129 Weights forcoil at the window position of −4 (Qian-Sejnowski, 1988); QIAN880130Weights for coil at the window position of −3 (Qian-Sejnowski, 1988);QIAN880131 Weights for coil at the window position of −2(Qian-Sejnowski, 1988); QIAN880132 Weights for coil at the windowposition of −1 (Qian-Sejnowski, 1988); QIAN880133 Weights for coil atthe window position of 0 (Qian-Sejnowski, 1988); QIAN880134 Weights forcoil at the window position of 1 (Qian-Sejnowski, 1988); QIAN880135Weights for coil at the window position of 2 (Qian-Sejnowski, 1988);QIAN880136 Weights for coil at the window position of 3 (Qian-Sejnowski,1988); QIAN880137 Weights for coil at the window position of 4(Qian-Sejnowski, 1988); QIAN880138 Weights for coil at the windowposition of 5 (Qian-Sejnowski, 1988); QIAN880139 Weights for coil at thewindow position of 6 (Qian-Sejnowski, 1988); RACS770101 Average reduceddistance for C-alpha (Rackovsky-Scheraga, 1977); RACS770102 Averagereduced distance for side chain (Rackovsky-Scheraga, 1977); RACS770103Side chain orientational preference (Rackovsky-Scheraga, 1977);RACS820101 Average relative fractional occurrence in A0(i)(Rackovsky-Scheraga, 1982); RACS820102 Average relative fractionaloccurrence in AR(i) (Rackovsky-Scheraga, 1982); RACS820103 Averagerelative fractional occurrence in AL(i) (Rackovsky-Scheraga, 1982);RACS820104 Average relative fractional occurrence in EL(i)(Rackovsky-Scheraga, 1982); RACS820105 Average relative fractionaloccurrence in E0(i) (Rackovsky-Scheraga, 1982); RACS820106 Averagerelative fractional occurrence in ER(i) (Rackovsky-Scheraga, 1982);RACS820107 Average relative fractional occurrence in A0(i−1)(Rackovsky-Scheraga, 1982); RACS820108 Average relative fractionaloccurrence in AR(i−1) (Rackovsky-Scheraga, 1982); RACS820109 Averagerelative fractional occurrence in AL(i−1) (Rackovsky-Scheraga, 1982);RACS820110 Average relative fractional occurrence in EL(i−1)(Rackovsky-Scheraga, 1982); RACS820111 Average relative fractionaloccurrence in E0(1-1) (Rackovsky-Scheraga, 1982); RACS820112 Averagerelative fractional occurrence in ER(i−1) (Rackovsky-Scheraga, 1982);RACS820113 Value of theta(i) (Rackovsky-Scheraga, 1982); RACS820114Value of theta(i−1) (Rackovsky-Scheraga, 1982); RADA880101 Transfer freeenergy from chx to wat (Radzicka-Wolfenden, 1988); RADA880102 Transferfree energy from oct to wat (Radzicka-Wolfenden, 1988); RADA880103Transfer free energy from vap to chx (Radzicka-Wolfenden, 1988);RADA880104 Transfer free energy from chx to oct (Radzicka-Wolfenden,1988); RADA880105 Transfer free energy from vap to oct(Radzicka-Wolfenden, 1988); RADA880106 Accessible surface area(Radzicka-Wolfenden, 1988); RADA880107 Energy transfer from out to in(95% buried) (Radzicka-Wolfenden, 1988); RADA880108 Mean polarity(Radzicka-Wolfenden, 1988); RICJ880101 Relative preference value at N″(Richardson-Richardson, 1988); RICJ880102 Relative preference value atN′ (Richardson-Richardson, 1988); RICJ880103 Relative preference valueat N-cap (Richardson-Richardson, 1988); RICJ880104 Relative preferencevalue at N1 (Richardson-Richardson, 1988); RICJ880105 Relativepreference value at N2 (Richardson-Richardson, 1988); RICJ880106Relative preference value at N3 (Richardson-Richardson, 1988);RICJ880107 Relative preference value at N4 (Richardson-Richardson,1988); RICJ880108 Relative preference value at N5(Richardson-Richardson, 1988); RICJ880109 Relative preference value atMid (Richardson-Richardson, 1988); RICJ880110 Relative preference valueat C5 (Richardson-Richardson, 1988); RICJ880111 Relative preferencevalue at C4 (Richardson-Richardson, 1988); RICJ880112 Relativepreference value at C3 (Richardson-Richardson, 1988); RICJ880113Relative preference value at C2 (Richardson-Richardson, 1988);RICJ880114 Relative preference value at C1 (Richardson-Richardson,1988); RICJ880115 Relative preference value at C-cap(Richardson-Richardson, 1988); RICJ880116 Relative preference value atC′ (Richardson-Richardson, 1988); RICJ880117 Relative preference valueat C″ (Richardson-Richardson, 1988); ROBB760101 Information measure foralpha-helix (Robson-Suzuki, 1976); ROBB760102 Information measure forN-terminal helix (Robson-Suzuki, 1976); ROBB760103 Information measurefor middle helix (Robson-Suzuki, 1976); ROBB760104 Information measurefor C-terminal helix (Robson-Suzuki, 1976); ROBB760105 Informationmeasure for extended (Robson-Suzuki, 1976); ROBB760106 Informationmeasure for pleated-sheet (Robson-Suzuki, 1976); ROBB760107 Informationmeasure for extended without H-bond (Robson-Suzuki, 1976); ROBB760108Information measure for turn (Robson-Suzuki, 1976); ROBB760109Information measure for N-terminal turn (Robson-Suzuki, 1976);ROBB760110 Information measure for middle turn (Robson-Suzuki, 1976);ROBB760111 Information measure for C-terminal turn (Robson-Suzuki,1976); ROBB760112 Information measure for coil (Robson-Suzuki, 1976);ROBB760113 Information measure for loop (Robson-Suzuki, 1976);ROBB790101 Hydration free energy (Robson-Osguthorpe, 1979); ROSG850101Mean area buried on transfer (Rose et al., 1985); ROSG850102 Meanfractional area loss (Rose et al., 1985); ROSM880101 Side chainhydropathy, uncorrected for solvation (Roseman, 1988); ROSM880102 Sidechain hydropathy, corrected for solvation (Roseman, 1988); ROSM880103Loss of Side chain hydropathy by helix formation (Roseman, 1988);SIMZ760101 Transfer free energy (Simon, 1976), Cited by Charton-Charton(1982); SNEP660101 Principal component I (Sneath, 1966); SNEP660102Principal component II (Sneath, 1966); SNEP660103 Principal componentIII (Sneath, 1966); SNEP660104 Principal component IV (Sneath, 1966);SUEM840101 Zimm-Bragg parameter s at 20 C (Sueki et al., 1984);SUEM840102 Zimm-Bragg parameter sigma ×1.0E4 (Sueki et al., 1984);SWER830101 Optimal matching hydrophobicity (Sweet-Eisenberg, 1983);TANS770101 Normalized frequency of alpha-helix (Tanaka-Scheraga, 1977);TANS770102 Normalized frequency of isolated helix (Tanaka-Scheraga,1977); TANS770103 Normalized frequency of extended structure(Tanaka-Scheraga, 1977); TANS770104 Normalized frequency of chainreversal R (Tanaka-Scheraga, 1977); TANS770105 Normalized frequency ofchain reversal S (Tanaka-Scheraga, 1977); TANS770106 Normalizedfrequency of chain reversal D (Tanaka-Scheraga, 1977); TANS770107Normalized frequency of left-handed helix (Tanaka-Scheraga, 1977);TANS770108 Normalized frequency of zeta R (Tanaka-Scheraga, 1977);TANS770109 Normalized frequency of coil (Tanaka-Scheraga, 1977)TANS770110 Normalized frequency of chain reversal (Tanaka-Scheraga,1977); VASM830101 Relative population of conformational state A (Vasquezet al., 1983); VASM830102 Relative population of conformational state C(Vasquez et al., 1983); VASM830103 Relative population of conformationalstate E (Vasquez et al., 1983); VELV850101 Electron-ion interactionpotential (Veljkovic et al., 1985); VENT840101 Bitterness (Venanzi,1984); VHEG790101 Transfer free energy to lipophilic phase (vonHeijne-Blomberg, 1979); WARP780101 Average interactions per side chainatom (Warme-Morgan, 1978); WEBA780101 RF value in high saltchromatography (Weber-Lacey, 1978); WERD780101 Propensity to be buriedinside (Wertz-Scheraga, 1978); WERD780102 Free energy change ofepsilon(i) to epsilon(ex) (Wertz-Scheraga, 1978); WERD780103 Free energychange of alpha(R1) to alpha(Rh) (Wertz-Scheraga, 1978); WERD780104 Freeenergy change of epsilon(i) to alpha(Rh) (Wertz-Scheraga, 1978);WOEC730101 Polar requirement (Woese, 1973); WOLR810101Hydrationpotential (Wolfenden et al., 1981); WOLS870101 Principal property valuez1 (Wold et al., 1987); WOLS870102 Principal property value z2 (Wold etal., 1987); WOLS870103 Principal property value z3 (Wold et al., 1987);YUTK870101 Unfolding Gibbs energy in water, pH7.0 (Yutani et al., 1987);YUTK870102 Unfolding Gibbs energy in water, pH9.0 (Yutani et al., 1987);YUTK870103 Activation Gibbs energy of unfolding, pH7.0 (Yutani et al.,1987); YUTK870104 Activation Gibbs energy of unfolding, pH9.0 (Yutani etal., 1987); ZASB820101 Dependence of partition coefficient on ionicstrength (Zaslaysky et al., 1982); ZIMJ680101 Hydrophobicity (Zimmermanet al., 1968); ZIMJ680102 Bulkiness (Zimmerman et al., 1968); ZIMJ680103Polarity (Zimmerman et al., 1968); ZIMJ680104 Isoelectric point(Zimmerman et al., 1968); ZIMJ680105 RF rank (Zimmerman et al., 1968);AURR980101 Normalized positional residue frequency at helix terminiN4′(Aurora-Rose, 1998); AURR980102 Normalized positional residuefrequency at helix termini N′″ (Aurora-Rose, 1998); AURR980103Normalizedpositional residue frequency at helix termini N″ (Aurora-Rose, 1998);AURR980104 Normalized positional residue frequency at helix terminiN′(Aurora-Rose, 1998); AURR980105 Normalized positional residuefrequency at helix termini Nc (Aurora-Rose, 1998); AURR980106 Normalizedpositional residue frequency at helix termini N1 (Aurora-Rose, 1998);AURR980107 Normalized positional residue frequency at helix termini N2(Aurora-Rose, 1998); AURR980108 Normalized positional residue frequencyat helix termini N3 (Aurora-Rose, 1998); AURR980109 Normalizedpositional residue frequency at helix termini N4 (Aurora-Rose, 1998);AURR980110 Normalized positional residue frequency at helix termini N5(Aurora-Rose, 1998); AURR980111 Normalized positional residue frequencyat helix termini C5 (Aurora-Rose, 1998); AURR980112 Normalizedpositional residue frequency at helix termini C4 (Aurora-Rose, 1998);AURR980113 Normalized positional residue frequency at helix termini C3(Aurora-Rose, 1998); AURR980114 Normalized positional residue frequencyat helix termini C2 (Aurora-Rose, 1998); AURR980115 Normalizedpositional residue frequency at helix termini C1 (Aurora-Rose, 1998);AURR980116 Normalized positional residue frequency at helix termini Cc(Aurora-Rose, 1998); AURR980117 Normalized positional residue frequencyat helix termini C′ (Aurora-Rose, 1998); AURR980118 Normalizedpositional residue frequency at helix termini C″ (Aurora-Rose, 1998);AURR980119 Normalized positional residue frequency at helix termini C′″(Aurora-Rose, 1998); AURR980120 Normalized positional residue frequencyat helix termini C4′ (Aurora-Rose, 1998); ONEK900101 Delta G values forthe peptides extrapolated to 0 M urea (O'Neil-DeGrado, 1990); ONEK900102Helix formation parameters (delta delta G) (O'Neil-DeGrado, 1990);VINM940101 Normalized flexibility parameters (B-values), average(Vihinen et al., 1994); VINM940102 Normalized flexibility parameters(B-values) for each residue surrounded by none rigid neighbours (Vihinenet al., 1994); VINM940103 Normalized flexibility parameters (B-values)for each residue surrounded by one rigid neighbours (Vihinen et al.,1994); VINM940104 Normalized flexibility parameters (B-values) for eachresidue surrounded by two rigid neighbours (Vihinen et al., 1994);MUNV940101 Free energy in alpha-helical conformation (Munoz-Serrano,1994); MUNV940102 Free energy in alpha-helical region (Munoz-Serrano,1994); MUNV940103 Free energy in beta-strand conformation(Munoz-Serrano, 1994); MUNV940104 Free energy in beta-strand region(Munoz-Serrano, 1994); MUNV940105 Free energy in beta-strand region(Munoz-Serrano, 1994) WIMW960101 Free energies of transfer of AcW1-X-LLpeptides from bilayer interface to water (Wimley-White, 1996);KIMC930101 Thermodynamic beta sheet propensity (Kim-Berg, 1993);MONM990101 Turn propensity scale for transmembrane helices (Monne etal., 1999); BLAM930101 Alpha helix propensity of position 44 in T4lysozyme (Blaber et al., 1993); PARS000101 p-Values of mesophilicproteins based on the distributions of B values (Parthasarathy-Murthy,2000); PARS000102 p-Values of thermophilic proteins based on thedistributions of B values (Parthasarathy-Murthy, 2000); KUMS000101Distribution of amino acid residues in the 18 non-redundant families ofthermophilic proteins (Kumar et al., 2000); KUMS000102 Distribution ofamino acid residues in the 18 non-redundant families of mesophilicproteins (Kumar et al., 2000); KUMS000103 Distribution of amino acidresidues in the alpha-helices in thermophilic proteins (Kumar et al.,2000); KUMS000104 Distribution of amino acid residues in thealpha-helices in mesophilic proteins (Kumar et al., 2000); TAKK010101Side-chain contribution to protein stability (kJ/mol) (Takano-Yutani,2001); FODMO20101 Propensity of amino acids within pi-helices(Fodje-Al-Karadaghi, 2002); NADH010101 Hydropathy scale based onself-information values in the two-state model (5% accessibility)(Naderi-Manesh et al., 2001); NADH010102 Hydropathy scale based onself-information values in the two-state model (9% accessibility)(Naderi-Manesh et al., 2001); NADH010103 Hydropathy scale based onself-information values in the two-state model (16% accessibility)(Naderi-Manesh et al., 2001); NADH010104 Hydropathy scale based onself-information values in the two-state model (20% accessibility)(Naderi-Manesh et al., 2001); NADH010105 Hydropathy scale based onself-information values in the two-state model (25% accessibility)(Naderi-Manesh et al., 2001); NADH010106 Hydropathy scale based onself-information values in the two-state model (36% accessibility)(Naderi-Manesh et al., 2001); NADH010107 Hydropathy scale based onself-information values in the two-state model (50% accessibility)(Naderi-Manesh et al., 2001); MONM990201 Averaged turn propensities in atransmembrane helix (Monne et al., 1999); KOEP990101 Alpha-helixpropensity derived from designed sequences (Koehl-Levitt, 1999);KOEP990102 Beta-sheet propensity derived from designed sequences(Koehl-Levitt, 1999); CEDJ970101 Composition of amino acids inextracellular proteins (percent) (Cedano et al., 1997); CEDJ970102Composition of amino acids in anchored proteins (percent) (Cedano etal., 1997); CEDJ970103 Composition of amino acids in membrane proteins(percent) (Cedano et al., 1997); CEDJ970104 Composition of amino acidsin intracellular proteins (percent) (Cedano et al., 1997); CEDJ970105Composition of amino acids in nuclear proteins (percent) (Cedano et al.,1997); FUKS010101 Surface composition of amino acids in intracellularproteins of thermophiles (percent) (Fukuchi-Nishikawa, 2001); FUKS010102Surface composition of amino acids in intracellular proteins ofmesophiles (percent) (Fukuchi-Nishikawa, 2001); FUKS010103 Surfacecomposition of amino acids in extracellular proteins of mesophiles(percent) (Fukuchi-Nishikawa, 2001); FUKS010104 Surface composition ofamino acids in nuclear proteins (percent) (Fukuchi-Nishikawa, 2001);FUKS010105 Interior composition of amino acids in intracellular proteinsof thermophiles (percent) (Fukuchi-Nishikawa, 2001); FUKS010106 Interiorcomposition of amino acids in intracellular proteins of mesophiles(percent) (Fukuchi-Nishikawa, 2001); FUKS010107 Interior composition ofamino acids in extracellular proteins of mesophiles (percent)(Fukuchi-Nishikawa, 2001); FUKS010108 Interior composition of aminoacids in nuclear proteins (percent) (Fukuchi-Nishikawa, 2001);FUKS010109 Entire chain composition of amino acids in intracellularproteins of thermophiles (percent) (Fukuchi-Nishikawa, 2001); FUKS010110Entire chain composition of amino acids in intracellular proteins ofmesophiles (percent) (Fukuchi-Nishikawa, 2001); FUKS010111 Entire chaincomposition of amino acids in extracellular proteins of mesophiles(percent) (Fukuchi-Nishikawa, 2001); FUKS010112 Entire chain compositionof amino acids in nuclear proteins (percent) (Fukuchi-Nishikawa, 2001);AVBF000101 Screening coefficients gamma, local (Avbelj, 2000);AVBF000102 Screening coefficients gamma, non-local (Avbelj, 2000);AVBF000103 Slopes tripeptide, FDPB VFF neutral (Avbelj, 2000);AVBF000104 Slopes tripeptides, LD VFF neutral (Avbelj, 2000); AVBF000105Slopes tripeptide, FDPB VFF noside (Avbelj, 2000); AVBF000106 Slopestripeptide FDPB VFF all (Avbelj, 2000); AVBF000107 Slopes tripeptideFDPB PARSE neutral (Avbelj, 2000); AVBF000108 Slopes dekapeptide, FDPBVFF neutral (Avbelj, 2000); AVBF000109 Slopes proteins, FDPB VFF neutral(Avbelj, 2000); YANJ020101 Side-chain conformation by gaussianevolutionary method (Yang et al., 2002); MITS020101 Amphiphilicity index(Mitaku et al., 2002); TSAJ990101 Volumes including the crystallographicwaters using the ProtOr (Tsai et al., 1999); TSAJ990102 Volumes notincluding the crystallographic waters using the ProtOr (Tsai et al.,1999); COSI940101 Electron-ion interaction potential values (Cosic,1994); PONP930101 Hydrophobicity scales (Ponnuswamy, 1993); WILM950101Hydrophobicity coefficient in RP-HPLC, C18 with 0.1% TFA/MeCN/H2O (Wilceet al. 1995); WILM950102 Hydrophobicity coefficient in RP-HPLC, C8 with0.1% TFA/MeCN/H2O (Wilce et al. 1995); WILM950103 Hydrophobicitycoefficient in RP-HPLC, C4 with 0.1% TFA/MeCN/H2O (Wilce et al. 1995);WILM950104 Hydrophobicity coefficient in RP-HPLC, C18 with 0.1%TFA/2-PrOH/MeCN/H2O (Wilce et al. 1995); KUHL950101 Hydrophilicity scale(Kuhn et al., 1995); GUOD860101 Retention coefficient at pH 2 (Guo etal., 1986); JURD980101 Modified Kyte-Doolittle hydrophobicity scale(Juretic et al., 1998); BASU050101 Interactivity scale obtained from thecontact matrix (Bastolla et al., 2005); BASU050102 Interactivity scaleobtained by maximizing the mean of correlation coefficient oversingle-domain globular proteins (Bastolla et al., 2005); BASU050103Interactivity scale obtained by maximizing the mean of correlationcoefficient over pairs of sequences sharing the TIM barrel fold(Bastolla et al., 2005); SUYM030101 Linker propensity index(Suyama-Ohara, 2003); PUNT030101 Knowledge-based membrane-propensityscale from 1D_Helix in MPtopo databases (Punta-Maritan, 2003);PUNT030102 Knowledge-based membrane-propensity scale from 3D_Helix inMPtopo databases (Punta-Maritan, 2003); GEOR030101 Linker propensityfrom all dataset (George-Heringa, 2003); GEOR030102 Linker propensityfrom 1-linker dataset (George-Heringa, 2003); GEOR030103 Linkerpropensity from 2-linker dataset (George-Heringa, 2003); GEOR030104Linker propensity from 3-linker dataset (George-Heringa, 2003);GEOR030105 Linker propensity from small dataset (linker length is lessthan six residues) (George-Heringa, 2003); GEOR030106 Linker propensityfrom medium dataset (linker length is between six and 14 residues)(George-Heringa, 2003); GEOR030107 Linker propensity from long dataset(linker length is greater than 14 residues) (George-Heringa, 2003);GEOR030108 Linker propensity from helical (annotated by DSSP) dataset(George-Heringa, 2003); GEOR030109 Linker propensity from non-helical(annotated by DSSP) dataset (George-Heringa, 2003); ZHOH040101 Thestability scale from the knowledge-based atom-atom potential (Zhou-Zhou,2004); ZHOH040102 The relative stability scale extracted from mutationexperiments (Zhou-Zhou, 2004); ZHOH040103 Buriability (Zhou-Zhou, 2004);BAEK050101 Linker index (Bae et al., 2005); HARY940101 Mean volumes ofresidues buried in protein interiors (Harpaz et al., 1994); PONJ960101Average volumes of residues (Pontius et al., 1996); DIGM050101Hydrostatic pressure asymmetry index, PAI (Di Giulio, 2005); WOLR790101Hydrophobicity index (Wolfenden et al., 1979); OLSK800101 Averageinternal preferences (Olsen, 1980); KIDA850101 Hydrophobicity-relatedindex (Kidera et al., 1985); GUYH850102 Apparent partition energiescalculated from Wertz-Scheraga index (Guy, 1985); GUYH850103 Apparentpartition energies calculated from Robson-Osguthorpe index (Guy, 1985);GUYH850104 Apparent partition energies calculated from Janin index (Guy,1985); GUYH850105 Apparent partition energies calculated from Chothiaindex (Guy, 1985); ROSM880104 Hydropathies of amino acid side chains,neutral form (Roseman, 1988); ROSM880105 Hydropathies of amino acid sidechains, pi-values in pH 7.0 (Roseman, 1988); JACR890101Weights from theIFH scale (Jacobs-White, 1989); COWR900101Hydrophobicity index, 3.0 pH(Cowan-Whittaker, 1990) BLAS910101 Scaled side chain hydrophobicityvalues (Black-Mould, 1991); CASG920101 Hydrophobicity scale from nativeprotein structures (Casari-Sippl, 1992); CORJ870101 NNEIG index(Cornette et al., 1987); CORJ870102 SWEIG index (Cornette et al., 1987);CORJ870103 PRIFT index (Cornette et al., 1987); CORJ870104 PRILS index(Cornette et al., 1987); CORJ870105 ALTFT index (Cornette et al., 1987)CORJ870106 ALTLS index (Cornette et al., 1987); CORJ870107 TOTFT index(Cornette et al., 1987); CORJ870108 TOTLS index (Cornette et al., 1987);MIYS990101 Relative partition energies derived by the Betheapproximation (Miyazawa-Jernigan, 1999); MIYS990102 Optimized relativepartition energies—method A (Miyazawa-Jernigan, 1999); MIYS990103Optimized relative partition energies—method B (Miyazawa-Jernigan,1999); MIYS990104 Optimized relative partition energies—method C(Miyazawa-Jernigan, 1999); MIYS990105 Optimized relative partitionenergies—method D (Miyazawa-Jernigan, 1999); ENGD860101 Hydrophobicityindex (Engelman et al., 1986); and FASG890101 Hydrophobicity index(Fasman, 1989)

In some embodiments of the invention, degenerate oligonucleotides areused to synthesize one or more of the TN1, DH, N2, and/or H3-JH segmentsof the invention. In certain embodiments of the invention, the codon ator near the 5′ end of the oligonucleotide encoding the H3-JH segment isa degenerate codon. Such degenerate codons may be the first codon fromthe 5′ end, the second codon from the 5′ end, the third codon from the5′ end, the fourth codon from the 5′ end, the fifth codon from the 5′end, and/or any combination of the above. In some embodiments of theinvention, one or more of the codons at or near the 5′ and/or 3′ ends ofthe DH segment are degenerate. Such degenerate codons may be the firstcodon from the 5′ and/or 3′ end(s), the second codon from the 5′ and/or3′ end(s), the third codons from the 5′ and/or 3′ end(s), the fourthcodon from the 5′ and/or 3′ end(s), the fifth codon from the 5′ and/or3′ end(s), and/or any combination of the above. Degenerate codons usedin each of the oligonucleotides encoding the segments may be selectedfor their ability to optimally recapitulate sequences in a theoreticalsegment pool and/or CDRH3 reference set.

In some embodiments, the invention provides methods of producing atheoretical segment pool of H3-JH segments, as described in theExamples. Theoretical segment pools generated utilizing NNN triplets,instead of or in addition to the NN doublets described in Example 5 alsofall within the scope of the invention, as do synthetic librariesincorporating segments from these theoretical segment pools.

In some embodiments, the invention provides methods of producing atheoretical segment pool of DH segments, as described in the Examples.In particular, for example, the invention provides methods of producinga theoretical segment pool of DH segments described by the PYTHONprogram of Example 6. Example 6 describes the application of thisprogram to produce the 68K theoretical segment pool (minimum length ofDNA sequences after progressive deletions=4 bases; and minimum length ofpeptide sequences for inclusion in the theoretical segment pool=2). Analternative example is provided wherein the minimum length of the DNAsequences after progressive deletions was one base and the minimumlength of the peptide sequence is one amino acid. It is alsocontemplated that other values could be used for these parameters. Forexample, the minimum length of the DNA sequences after progressivedeletions could be set as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,or 15, and the minimum length of the peptide sequences in thetheoretical segment pool could be set as 1, 2, 3, 4, or 5.

Design of CDRH3 Libraries Using the TN1, DH, N2, and H3-JH Segments

The CDRH3 libraries of the invention comprise TN1, DH, N2, and H3-JHsegments. Thus, in certain embodiments of the invention, the overalldesign of the CDRH3 libraries can be represented by the followingformula:[TN1]-[DH]-[N2]-[H3-JH].

In certain embodiments of the invention, a synthetic CDRH3 repertoire iscombined with selected VH chassis sequences and heavy chain constantregions, via homologous recombination. Therefore, in certain embodimentsof the invention, it may be desirable to include DNA sequences flankingthe 5′ and 3′ ends of the synthetic CDRH3 libraries, to facilitatehomologous recombination between the synthetic CDRH3 libraries andvectors containing the selected chassis and constant regions. In certainembodiments, the vectors also contain a sequence encoding at least aportion of the non-truncated region of the IGHJ gene (i.e., FRM4-JH).Thus, a polynucleotide encoding an N-terminal sequence (e.g., CA(K/R/T))may be added to the synthetic CDRH3 sequences, wherein the N-terminalpolynucleotide is homologous with FRM3 of the chassis, while apolynucleotide encoding a C-terminal sequence (e.g., WG(Q/R/K)G) may beadded to the synthetic CDRH3, wherein the C-terminal polynucleotide ishomologous with FRM4-JH. Although the sequence WG(Q/R)G is presented inthis exemplary embodiment, additional amino acids, C-terminal to thissequence in FRM4-JH may also be included in the polynucleotide encodingthe C-terminal sequence. The purpose of the polynucleotides encoding theN-terminal and C-terminal sequences, in this case, is to facilitatehomologous recombination, and one of ordinary skill in the art wouldrecognize that these sequences may be longer or shorter than depictedbelow. Accordingly, in certain embodiments of the invention, the overalldesign of the CDRH3 repertoire, including the sequences required tofacilitate homologous recombination with the selected chassis, can berepresented by the following formula (regions homologous with vectorunderlined):

CA[R/K/T]-[TN1]-[DH]-[N2]-[H3-JH]-[WG(Q/R/K)G] (SEQ ID NO. 8762, SEQ IDNO. 8763, and SEQ ID NO. 8764, respectively).

In some embodiments of the invention, the CDRH3 repertoire can berepresented by the following formula, which excludes the T residuepresented in the schematic above:

CA[R/K]-[TN1]-[DH]-[N2]-[H3-JH]-[WG(Q/R/K)G] (SEQ ID NO. 8762, SEQ IDNO. 8763, and SEQ ID NO. 8764, respectively).

References describing collections of V, D, and J genes include Scavineret al., Exp. Clin, Immunogenet., 1999, 16: 243 and Ruiz et al., Exp.Clin. Immunogenet, 1999, 16: 173, each incorporated by reference in itsentirety.

Although homologous recombination is one method of producing thelibraries of the invention, a person of ordinary skill in the art willreadily recognize that other methods of DNA assembly, such as ligationor site-specific recombination, and/or DNA synthesis, can also be usedto produce the libraries of the invention.

CDRH3 Lengths

The lengths of the segments may also be varied, for example, to producelibraries with a particular distribution of CDRH3 lengths. In oneembodiment of the invention, the H3-JH segments are about 0 to about 10amino acids in length, the DH segments are about 0 to about 12 aminoacids in length, the TN1 segments are about 0 to about 4 amino acids inlength, and the N2 segments are about 0 to about 4 amino acids inlength. In certain embodiments, the H3-JH segments are at least about 0,1, 2, 3, 4, 5, 6, 7, 8, 9, and/or 10 amino acids in length. In someembodiments, the DH segments are at least about 0, 1, 2, 3, 4, 5, 6, 7,8, 9, 10, 11, and/or 12 amino acids in length. In certain embodiments,the TN1 segments are at least about 0, 1, 2, 3, or 4 amino acids inlength. In some embodiments, the N2 amino acids are at least about 0, 1,2, 3, or 4 amino acids in length. In certain embodiments of theinvention, the CDRH3 is about 2 to about 35, about 2 to about 28, orabout 5 to about 26 amino acids in length. In some embodiments, theCDRH3 is at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, and/or 35 amino acids in length. In some embodiments, the length ofany of the segments or CDRH3s of the invention may be less than aparticular number of amino acids, where the number of amino acids isdefined using any one of the integers provided above for the respectivesegment or CDRH3. In certain embodiments of the invention, a particularnumerical range is defined, using any two of the integers provided aboveas lower and upper boundaries of the range, inclusive or exclusive. Allcombinations of the integers provided, which define an upper and lowerboundary, are contemplated.

Design of CDRL3 Libraries

The design of CDRL3 libraries, and light chain sequences, is describedin detail in U.S. Publication Nos. 2009/0181855 and 2010/0056386, andWO/2009/036379, each of which is incorporated by reference in itsentirety, and is therefore only described briefly herein. Librariesdescribed herein are designed according to similar principles, withthree important differences, namely that the libraries of the currentinvention contain (1) variability in CDRL1 and CDRL2; (2) variability inthe framework regions; and/or (3) variability in CDRL3 that is designedto produce light chain libraries with CDRL3s that closely resemble humangermline-like CDRL3 sequences, as defined above (Table 1).

A CDRL3 library of the invention may be a VKCDR3 library and/or a VλCDR3library. In certain embodiments of the invention, patterns of occurrenceof particular amino acids at defined positions within VL sequences aredetermined by analyzing data available in public or other databases, forexample, the NCBI database (see, for example, WO/2009/036379). Incertain embodiments of the invention, these sequences are compared onthe basis of identity and assigned to families on the basis of thegermline genes from which they are derived. The amino acid compositionat each position of the sequence, in each germline family, may then bedetermined. This process is illustrated in the Examples provided herein.

Light Chains with Framework Variability

In some embodiments, the invention provides a library of light chainvariable domains wherein the light chain variable domains are varied atone or more of framework positions 2, 4, 36, 46, 48, 49, and 66. In someembodiments, the invention provides a library of light chain variabledomains comprising at least a plurality of light chain variable domainswhose amino acid sequences are identical to one another except forsubstitutions at one or more of positions 2, 4, 36, 46, 48, 49, and 66.In certain embodiments, the invention provides a library of light chainvariable domains comprising at least a plurality of light chain variabledomains whose amino acid sequences are at least about 70%, 75%, 80%,85%, 90%, 95%, 96%, 97%, 98%, 99%, and/or 99.5% to any of the lightchain variable domain sequences disclosed herein, and further havesubstitutions at one or more of positions 2, 4, 36, 46, 48, 49, and 66.In some embodiments, the amino acids selected for inclusion in thesepositions are selected from amongst about the most 2, 3, 4, 5, 6, 7, 8,9, and/or 10 most frequently occurring amino acids at the correspondingposition in a reference set of light chain variable domains.

In some embodiments, the invention provides systems and methods ofselecting framework positions to be varied in a light chain variabledomain, comprising:

-   -   (i) obtaining a reference set of light chain sequences, wherein        the reference set contains light chain sequences with VL        segments selected from the group consisting of sequences found        in, or encoded by, a single IGVL germline gene and/or sequences        found in, or encoded by, allelic variants of the single IGVL        germline gene;    -   (ii) determining which framework positions within the reference        set have a degree of variability that is similar to the degree        of variability occurring in one more CDR positions of the        sequences in the reference set (e.g., the variability in a        framework position is at least about 70%, 80%, 90%, or 95%,        100%, or more of the variability found in a CDR position of the        sequences in the reference set);    -   (iii) determining the frequency of occurrence of amino acid        residues for each of the framework positions identified in (ii);    -   (iv) synthesizing light chain variable domain encoding sequences        wherein the framework positions identified in (ii) are varied to        include the 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,        17, 18, 19, or 20 most frequently occurring amino acid residues        (identified in (iii)) at the corresponding position.

One of ordinary skill in the art, reading the present disclosure willappreciate that the present invention provides analogous methods fordeveloping framework variants of heavy chain sequences.

Light Chains with CDR1 and/or CDR2Variability

In some embodiments, the invention provides a library of light chainvariable domains wherein the light chain variable domains are varied atone or more of CDRL1 positions 28, 29, 30, 30A, 30B, 30E, 31, and 32(Chothia-Lesk numbering scheme; Chothia and Lesk, J. Mol. Biol., 1987,196: 901). In some embodiments, the invention provides a library oflight chain variable domains wherein the light chain variable domainsare varied at one or more of CDRL2 positions 50, 51, 53, and 55. In someembodiments, the amino acids selected for inclusion in these CDRL1and/or CDRL2 positions are selected from amongst about the most 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and/or 20 mostfrequently occurring amino acids at the corresponding position in areference set of light chain variable domains.

In some embodiments, the invention provides systems and methods forselecting CDRL1 and/or CDRL2 positions to be varied in a light chainvariable domain, comprising:

-   -   (i) obtaining a reference set of light chain sequences, wherein        the reference set contains light chain sequences with VL        segments selected from the group consisting of sequences found        in, or encoded by, a single IGVL germline gene and sequences        found in, or encoded by, allelic variants of the single IGVL        germline gene;    -   (ii) determining which CDRL1 and/or CDRL2 positions are variable        within the reference set;    -   (iii) synthesizing light chain variable domain encoding        sequences wherein the CDRL1 and/or CDRL2 positions identified        in (ii) are varied to include the 2, 3, 4, 5, 6, 7, 8, 9, 10,        11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 most frequently        occurring amino acid residues at the corresponding position.

One of ordinary skill in the art, reading the present disclosure willappreciate that the present invention provides analogous methods fordeveloping CDRH2 and/or CDRH2 variants of heavy chain sequences.

Light Chain Sequences

In some embodiments, the invention provides a light chain librarycomprising one or more of any of the light chain sequences providedherein, for example, the polypeptide sequences of Table 3 and/or Table 4and/or the polynucleotide sequences of Table 5, Table 6, and/or Table 7.A person of ordinary skill in the art will recognize that not everylight chain sequence provided herein is necessary to produce afunctional light chain library of the invention. Therefore, in certainembodiments, a light chain library of the invention will contain asubset of the sequences described above. For example, in certainembodiments of the invention, at least about 10, 100, 200, 300, 400,500, 600, 700, 800, 900, 10³, 10⁴, and/or 10⁵ of the light chainpolynucleotide and/or polypeptide sequences provided herein are includedin a library. In some embodiments, a library of the invention maycontain less than a particular number of polynucleotide or polypeptidesegments, where the number of segments is defined using any one of theintegers provided above for the respective segment. In certainembodiments of the invention, a particular numerical range is defined,using any two of the integers provided above as lower and upperboundaries of the range, inclusive or exclusive. All combinations of theintegers provided, which define an upper and lower boundary, arecontemplated.

In certain embodiments, the invention provides light chain librariescomprising at least about 1%, 2.5%, 5%, 10%, 15%, 20%, 25%, 30%, 40%,45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% of thesequences from any of the sets of light chain sequences provided herein.For example, the invention provides libraries comprising at least about1%, 2.5%, 5%, 10%, 15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%,70%, 75%, 80%, 85%, 90%, 95%, or 99% of the light chain sequencesprovided in Table 3, Table 4, Table 5, Table 6, and/or Table 7. In someembodiments of the invention, a particular percentage range is defined,using any two of the percentages provided above as lower and upperboundaries of the range, inclusive or exclusive. All combinations of thepercentages provided, which define an upper and lower boundary, arecontemplated.

In some embodiments of the invention, at least about 1%, 2.5%, 5%, 10%,15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%,90%, 95%, or 99% of the light chain sequences in a library are lightchain sequences provided herein. In certain embodiments of theinvention, at least about 1%, 2.5%, 5%, 10%, 15%, 20%, 25%, 30%, 40%,45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% of thelight chain sequences isolated from a light chain library (e.g., bybinding to a particular antigen and/or generic ligand) are light chainsequences provided herein. In some embodiments, a light chain library ofthe invention may contain less than a particular percentage of lightchain sequences provided herein, where the percentage of light chainsequences is defined using any one of the percentages provided above. Incertain embodiments of the invention, a particular percentage range isdefined, using any two of the percentages provided above as lower andupper boundaries of the range, inclusive or exclusive. All combinationsof the percentages provided, which define an upper and lower boundary,are contemplated.

One of ordinary skill in the art will further recognize that given thelight chain sequences provided herein, similar light chain sequencescould be produced which share a designated level of overall sequenceidentity and/or one or more characteristic sequence elements describedherein, which overall degree of sequence identity and/or characteristicsequence elements may confer common functional attributes. Those ofordinary skill in the art will be well familiar with a variety oftechniques for preparing such related sequences, including themutagenesis techniques provided herein. Therefore, each of theexplicitly enumerated embodiments of the invention can also be practicedusing light chain sequences that share a particular percent identity toany of the light chain sequences provided herein. For example, each ofthe previously described embodiments of the invention can be practicedusing light chain sequences that are at least about 70%, 75%, 80%, 85%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 99.9%identical to the light chain sequences provided herein. For example, insome embodiments, light chain libraries provided by the inventioncomprise light chain variable domains at least about 70%, 75%, 80%, 85%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 99.9%identical to the light chain sequences provided herein, withsubstitutions in one or more of framework positions 2, 4, 36, 46, 48,49, and 66, CDRL1 positions 28, 29, 30, 30A, 30B, 30E, 31, and 32(Chothia-Lesk numbering scheme), and/or CDRL2 positions 50, 51, 53, and55.

In some embodiments, the invention provides systems and methods forvarying positions within the portion of CDRL3s encoded by a particularIGVL germline gene, comprising:

-   -   (i) obtaining a reference set of light chain sequences, wherein        the reference set contains light chain sequences with VL        segments originating from the same IGVL germline gene and/or its        allelic variants;    -   (ii) determining which amino acids occur at each of the CDRL3        positions in the reference set that are encoded by the IGVL gene        (i.e., positions 89-94, inclusive);    -   (iii) synthesizing light chain variable domain encoding        sequences wherein two positions in each light chain variable        domain encoding sequence contain degenerate codons encoding the        2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,        or 20 most frequently occurring amino acid residues at the        corresponding positions in the reference set.

As described in the examples, the degenerate codons of (iii) can bechosen to best reproduce the amino acid diversity contained in thereference set for each of the two positions varied in each light chain.Finally, while the methods and systems described above are describedwith respect to CDRL3, one of ordinary skill in the art will readilyrecognize that the same principles can be applied to CDRH1 and/or CDRH2of the heavy chain, which are encoded entirely by the IGHV gene.

CDRL3 Lengths

In some embodiments, as an alternative or in addition to other featuresdescribed herein, the present invention provides libraries in whichlengths of CDRL3s may be varied. The present invention thereforeprovides, among other things, libraries with a particular distributionof CDRL3 lengths. Although CDRL3 libraries of lengths 8, 9, and 10 areexemplified, one of ordinary skill in the art will readily recognizethat the methods described herein can be applied to produce light chainswith CDRL3s of different lengths (e.g., about 5, 6, 7, 11, 12, 13, 14,15, and/or 16) that also fall within the scope of the invention. In someembodiments, the length of any of the CDRL3s of the invention may beless than a particular number of amino acids, where the number of aminoacids is defined using any one of the integers provided above. In someembodiments of the invention, a particular numerical range is defined,using any two of the integers provided above as lower and upperboundaries of the range, inclusive or exclusive. All combinations of theintegers provided, which define an upper and lower boundary, arecontemplated.

Synthetic Antibody Libraries

In some embodiments of the invention, provided libraries include one ormore synthetic polynucleotides. In some embodiments, provided librariesmay comprise synthetic polynucleotides selected from (a) heavy chainchassis polynucleotides; (b) light chain chassis polynucleotides; (c)CDR3 polynucleotides; (d) constant domain polynucleotides; and (e)combinations thereof. Those of ordinary skill in the art will appreciatethat such synthetic polynucleotides may be linked to other synthetic ornon-synthetic polynucleotides in provided libraries.

Synthetic polynucleotides provided herein may be prepared by anyavailable method. For example, in some embodiments, syntheticpolynucleotides can be synthesized by split pool DNA synthesis asdescribed in Feldhaus et al., Nucleic Acids Research, 2000, 28: 534;Omstein et al., Biopolymers, 1978, 17: 2341; Brenner and Lerner, PNAS,1992, 87: 6378, U.S. Publication Nos. 2009/0181855 and 2010/0056386, andWO/2009/036379 (each incorporated by reference in its entirety).

In some embodiments of the invention, segments representing the possibleTN1, DH, N2, and JH diversity found in the human repertoire aresynthesized de novo either as double-stranded DNA oligonucleotides,single-stranded DNA oligonucleotides representative of the codingstrand, or single-stranded DNA oligonucleotides representative of thenon-coding strand. Such sequences can then be introduced into a hostcell along with an acceptor vector containing a chassis sequence and, insome cases a portion of FRM4 and a constant region. No primer-based PCRamplification from mammalian cDNA or mRNA or template-directed cloningsteps from mammalian cDNA or mRNA need be employed.

Construction of Libraries by Yeast Homologous Recombination

In certain embodiments, the invention exploits the inherent ability ofyeast cells to facilitate homologous recombination at high efficiency.The mechanism of homologous recombination in yeast and its applicationsare briefly described below (also see e.g., U.S. Pat. Nos. 6,406,863;6,410,246; 6,410,271; 6,610,472; and 7,700,302, each of which isincorporated by reference in its entirety).

As an illustrative embodiment, homologous recombination can be carriedout in, for example, Saccharomyces cerevisiae, which has geneticmachinery designed to carry out homologous recombination with highefficiency. Exemplary S. cerevisiae strains include EM93, CEN.PK2,RM11-1a, YJM789, and BJ5465. This mechanism is believed to have evolvedfor the purpose of chromosomal repair, and is also called “gap repair”or “gap filling”. By exploiting this mechanism, mutations can beintroduced into specific loci of the yeast genome. For example, a vectorcarrying a mutant gene can contain two sequence segments that arehomologous to the 5′ and 3′ open reading frame (ORF) sequences of a genethat is intended to be interrupted or mutated. The vector may alsoencode a positive selection marker, such as a nutritional enzyme allele(e.g., URA3) and/or an antibiotic resistant marker (e.g.,Geneticin/G418), flanked by the two homologous DNA segments. Otherselection markers and antibiotic resistance markers are known to one ofordinary skill in the art.

In some embodiments of the invention, this vector (e.g., a plasmid) islinearized and transformed into the yeast cells. Through homologousrecombination between the plasmid and the yeast genome, at the twohomologous recombination sites, a reciprocal exchange of the DNA contentoccurs between the wild type gene in the yeast genome and the mutantgene (including the selection marker gene(s)) that is flanked by the twohomologous sequence segments. By selecting for the one or more selectionmarkers, the surviving yeast cells will be those cells in which thewild-type gene has been replaced by the mutant gene (Pearson et al.,Yeast, 1998, 14: 391, incorporated by reference in its entirety). Thismechanism has been used to make systematic mutations in all 6,000 yeastgenes, or open reading frames (ORFs), for functional genomics studies.Because the exchange is reciprocal, a similar approach has also beenused successfully to clone yeast genomic DNA fragments into a plasmidvector (Iwasaki et al., Gene, 1991, 109: 81, incorporated by referencein its entirety).

By utilizing the endogenous homologous recombination machinery presentin yeast, gene fragments or synthetic oligonucleotides can also becloned into a plasmid vector without a ligation step. In thisapplication of homologous recombination, a target gene fragment (i.e.,the fragment to be inserted into a plasmid vector, e.g., a CDR3) isobtained (e.g., by oligonucleotides synthesis, PCR amplification,restriction digestion out of another vector, etc.). DNA sequences thatare homologous to selected regions of the plasmid vector are added tothe 5′ and 3′ ends of the target gene fragment. These homologous regionsmay be fully synthetic, or added via PCR amplification of a target genefragment with primers that incorporate the homologous sequences. Theplasmid vector may include a positive selection marker, such as anutritional enzyme allele (e.g., URA3), or an antibiotic resistancemarker (e.g., Geneticin/G418). The plasmid vector is then linearized bya unique restriction cut located in-between the regions of sequencehomology shared with the target gene fragment, thereby creating anartificial gap at the cleavage site. The linearized plasmid vector andthe target gene fragment flanked by sequences homologous to the plasmidvector are co-transformed into a yeast host strain. The yeast is thenable to recognize the two stretches of sequence homology between thevector and target gene fragment and facilitate a reciprocal exchange ofDNA content through homologous recombination at the gap. As aconsequence, the target gene fragment is inserted into the vectorwithout ligation.

The method described above has also been demonstrated to work when thetarget gene fragments are in the form of single stranded DNA, forexample, as a circular M13 phage derived form, or as single strandedoligonucleotides (Simon and Moore, Mol. Cell. Biol., 1987, 7: 2329;Ivanov et al., Genetics, 1996, 142: 693; and DeMarini et al., 2001, 30:520., each incorporated by reference in its entirety). Thus, the form ofthe target that can be recombined into the gapped vector can be doublestranded or single stranded, and derived from chemical synthesis, PCR,restriction digestion, or other methods.

Several factors may influence the efficiency of homologous recombinationin yeast. For example, the efficiency of the gap repair is correlatedwith the length of the homologous sequences flanking both the linearizedvector and the target gene. In certain embodiments, about 20 or morebase pairs may be used for the length of the homologous sequence, andabout 80 base pairs may give a near-optimized result (Hua et al.,Plasmid, 1997, 38: 91; Raymond et al., Genome Res., 2002, 12: 190, eachincorporated by reference in its entirety). In certain embodiments ofthe invention, at least about 5, 10, 15, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34 35, 36, 37, 38, 39, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180,187, 190, or 200 homologous base pairs may be used to facilitaterecombination. In certain embodiments, between about 20 and about 40base pairs are utilized. In addition, the reciprocal exchange betweenthe vector and gene fragment is strictly sequence-dependent, i.e. itdoes not cause a frame shift. Therefore, gap-repair cloning assures theinsertion of gene fragments with both high efficiency and precision. Thehigh efficiency makes it possible to clone two, three, or more targetedgene fragments simultaneously into the same vector in one transformationattempt (Raymond et al., Biotechniques, 1999, 26: 134, incorporated byreference in its entirety). Moreover, the nature of precision sequenceconservation through homologous recombination makes it possible to cloneselected genes or gene fragments into expression or fusion vectors fordirect functional examination (El-Deiry et al., Nature Genetics, 1992,1: 4549; Ishioka et al., PNAS, 1997, 94: 2449, each incorporated byreference in its entirety).

Libraries of gene fragments have also been constructed in yeast usinghomologous recombination. For example, a human brain cDNA library wasconstructed as a two-hybrid fusion library in vector pJG4-5 (Guidottiand Zervos, Yeast, 1999, 15: 715, incorporated by reference in itsentirety). It has also been reported that a total of 6,000 pairs of PCRprimers were used for amplification of 6,000 known yeast ORFs for astudy of yeast genomic protein interactions (Hudson et al., Genome Res.,1997, 7: 1169, incorporated by reference in its entirety). In 2000, Uetzet al. conducted a comprehensive analysis-of protein-proteininteractions in Saccharomyces cerevisiae (Uetz et al., Nature, 2000,403: 623, incorporated by reference in its entirety). Theprotein-protein interaction map of the budding yeast was studied byusing a comprehensive system to examine two-hybrid interactions in allpossible combinations between the yeast proteins (Ito et al., PNAS,2000, 97: 1143, incorporated by reference in its entirety), and thegenomic protein linkage map of Vaccinia virus was studied using thissystem (McCraith et al., PNAS, 2000, 97: 4879, incorporated by referencein its entirety).

In certain embodiments of the invention, a synthetic CDR3 (heavy orlight chain) may be joined by homologous recombination with a vectorencoding a heavy or light chain chassis, a portion of FRM4, and aconstant region, to form a full-length heavy or light chain. In certainembodiments of the invention, the homologous recombination is performeddirectly in yeast cells. In some embodiments, such a method comprises:

-   -   (a) transforming into yeast cells:        -   (i) a linearized vector encoding a heavy or light chain            chassis, a portion of FRM4, and a constant region, wherein            the site of linearization is between the end of FRM3 of the            chassis and the beginning of the constant region; and        -   (ii) a library of CDR3 insert nucleotide sequences that are            linear and double stranded, wherein each of the CDR3 insert            sequences comprises a nucleotide sequence encoding CDR3 and            5′- and 3′-flanking sequences that are sufficiently            homologous to the termini of the vector of (i) at the site            of linearization to enable homologous recombination to occur            between the vector and the library of CDR3 insert sequences;            and    -   (b) allowing homologous recombination to occur between the        vector and the CDR3 insert sequences in the transformed yeast        cells, such that the CDR3 insert sequences are incorporated into        the vector, to produce a vector encoding full-length heavy chain        or light chain.

As specified above, CDR3 inserts may have a 5′ flanking sequence and a3′ flanking sequence that are homologous to the termini of thelinearized vector. When the CDR3 inserts and the linearized vectors areintroduced into a host cell, for example, a yeast cell, the “gap” (thelinearization site) created by linearization of the vector is filled bythe CDR3 fragment insert through recombination of the homologoussequences at the 5′ and 3′ termini of these two linear double-strandedDNAs (i.e., the vector and the insert). Through this event of homologousrecombination, libraries of circular vectors encoding full-length heavyor light chains comprising variable CDR3 inserts is generated.Particular instances of these methods are presented in the Examples.

Subsequent analysis may be carried out to determine, for example, theefficiency of homologous recombination that results in correct insertionof the CDR3 sequences into the vectors. For example, PCR amplificationof the CDR3 inserts directly from selected yeast clones may reveal howmany clones are recombinant. In certain embodiments, libraries withminimum of about 90% recombinant clones are utilized. In certainembodiments libraries with a minimum of about 1%, 5% 10%, 15%, 20%, 25%,30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%recombinant clones are utilized. The same PCR amplification of selectedclones may also reveal the insert size.

To verify the sequence diversity of the inserts in the selected clones,a PCR amplification product with the correct size of insert may be“fingerprinted” with restriction enzymes known to cut or not cut withinthe amplified region. From a gel electrophoresis pattern, it may bedetermined whether the clones analyzed are of the same identity or ofthe distinct or diversified identity. The PCR products may also besequenced directly to reveal the identity of inserts and the fidelity ofthe cloning procedure, and to prove the independence and diversity ofthe clones.

Expression and Screening Systems

Libraries of polynucleotides generated by any of the techniquesdescribed herein, or other suitable techniques, can be expressed andscreened to identify antibodies having desired structure and/oractivity. Expression of the antibodies can be carried out, for example,using cell-free extracts (and e.g., ribosome display), phage display,prokaryotic cells (e.g., bacterial display), or eukaryotic cells (e.g.,yeast display). In certain embodiments of the invention, the antibodylibraries are expressed in yeast.

In some embodiments, polynucleotides are engineered to serve astemplates that can be expressed in a cell-free extract. Vectors andextracts as described, for example in U.S. Pat. Nos. 5,324,637;5,492,817; 5,665,563, (each incorporated by reference in its entirety)can be used and many are commercially available. Ribosome display andother cell-free techniques for linking a polynucleotide (i.e., agenotype) to a polypeptide (i.e., a phenotype) can be used, e.g.,Profusion™ (see, e.g., U.S. Pat. Nos. 6,348,315; 6,261,804; 6,258,558;and 6,214,553, each incorporated by reference in its entirety).

Alternatively or additionally, polynucleotides of the invention can beexpressed in an E. coli expression system, such as that described byPluckthun and Skerra. (Meth. Enzymol., 1989, 178: 476; Biotechnology,1991, 9: 273, each incorporated by reference in its entirety). Mutantproteins can be expressed for secretion in the medium and/or in thecytoplasm of the bacteria, as described by Better and Horwitz, Meth.Enzymol., 1989, 178: 476, incorporated by reference in its entirety. Insome embodiments, the single domains encoding VH and VL are eachattached to the 3′ end of a sequence encoding a signal sequence, such asthe ompA, phoA or pelB signal sequence (Lei et al., J. Bacteriol., 1987,169: 4379, incorporated by reference in its entirety). These genefusions are assembled in a dicistronic construct, so that they can beexpressed from a single vector, and secreted into the periplasmic spaceof E. coli where they will refold and can be recovered in active form.(Skerra et al., Biotechnology, 1991, 9: 273, incorporated by referencein its entirety). For example, antibody heavy chain genes can beconcurrently expressed with antibody light chain genes to produceantibodies or antibody fragments.

In some embodiments of the invention, antibody sequences are expressedon the membrane surface of a prokaryote, e.g., E. coli, using asecretion signal and lipidation moiety as described, e.g., inUS2004/0072740; US2003/0100023; and US2003/0036092 (each incorporated byreference in its entirety).

Higher eukaryotic cells, such as mammalian cells, for example myelomacells (e.g., NS/0 cells), hybridoma cells, Chinese hamster ovary (CHO),and human embryonic kidney (HEK) cells, can also be used for expressionof the antibodies of the invention. Typically, antibodies expressed inmammalian cells are designed to be secreted into the culture medium, orexpressed on the surface of the cell. Antibody or antibody fragments canbe produced, for example, as intact antibody molecules or as individualVH and VL fragments, Fab fragments, single domains, or as single chains(scFv) (Huston et al., PNAS, 1988, 85: 5879, incorporated by referencein its entirety).

Alternatively or additionally, antibodies can be expressed and screenedby anchored periplasmic expression (APEx 2-hybrid surface display), asdescribed, for example, in Jeong et al., PNAS, 2007, 104: 8247(incorporated by reference in its entirety) or by other anchoringmethods as described, for example, in Mazor et al., NatureBiotechnology, 2007, 25: 563 (incorporated by reference in itsentirety).

In some embodiments of the invention, antibodies can be selected usingmammalian cell display (Ho et al., PNAS, 2006, 103: 9637, incorporatedby reference in its entirety).

Screening of the antibodies derived from the libraries of the inventioncan be carried out by any appropriate means. For example, bindingactivity can be evaluated by standard immunoassay and/or affinitychromatography. Screening of antibodies of the invention for catalyticfunction, e.g., proteolytic function can be accomplished using astandard assays, e.g., the hemoglobin plaque assay as described in U.S.Pat. No. 5,798,208 (incorporated by reference in its entirety).Determining the ability of candidate antibodies to bind therapeutictargets can be assayed in vitro using, e.g., a BIACORE™ instrument,which measures binding rates of an antibody to a given target or antigenbased on surface plasmon resonance. In vivo assays can be conductedusing any of a number of animal models and then subsequently tested, asappropriate, in humans. Cell-based biological assays are alsocontemplated.

One feature of the instant invention is the speed at which theantibodies of the library can be expressed and screened. In certainembodiments of the invention, the antibody library can be expressed inyeast, which have a doubling time of less than about 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours.In some embodiments, the doubling times are about 1 to about 3 hours,about 2 to about 4, about 3 to about 8 hours, about 3 to about 24, about5 to about 24, about 4 to about 6 about 5 to about 22, about 6 to about8, about 7 to about 22, about 8 to about 10 hours, about 7 to about 20,about 9 to about 20, about 9 to about 18, about 11 to about 18, about 11to about 16, about 13 to about 16, about 16 to about 20, or about 20 toabout 30 hours. In certain embodiments of the invention, an antibodylibrary is expressed in yeast with a doubling time of about 16 to about20 hours, about 8 to about 16 hours, or about 4 to about 8 hours. Thus,an antibody library of the instant invention can be expressed andscreened in a matter of hours, as compared to previously knowntechniques which take several days to express and screen antibodylibraries. A limiting step in the throughput of such screening processesin mammalian cells is typically the time required to iteratively regrowpopulations of isolated cells, which, in some cases, have doubling timesgreater than the doubling times of the yeast used in the currentinvention.

In certain embodiments of the invention, the composition of a librarymay be defined after one or more enrichment steps (for example byscreening for antigen binding, binding to a generic ligand, or otherproperties). For example, a library with a composition comprising about×% sequences or libraries of the invention may be enriched to containabout 2×%, 3×%, 4×%, 5×%, 6×%, 7×%, 8×%, 9×%, 10×%, 20×%, 25×%, 40×%,50×%, 60×% 75×%, 80×%, 90×%, 95×%, or 99×% sequences or libraries of theinvention, after one or more screening steps. In some embodiments of theinvention, the sequences or libraries of the invention may be enrichedabout 2-fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold, 9-fold,10-fold, 100-fold, 1,000-fold, or more, relative to their occurrenceprior to the one or more enrichment steps. In certain embodiments of theinvention, a library may contain at least a certain number of aparticular type of sequence(s), such as CDRH3s, CDRL3s, heavy chains,light chains, or whole antibodies (e.g., at least about 10³, 10⁴, 10⁵,10⁶, 10⁷, 10⁸, 10⁹, 10¹⁰, 10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵, 10¹⁶, 10¹⁷,10¹⁸, 10¹⁹, or 10²⁰). In certain embodiments, these sequences may beenriched during one or more enrichment steps, to provide librariescomprising at least about 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, 10¹⁰,10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵, 10¹⁶, 10¹⁷, 10¹⁸, or 10¹⁹ of therespective sequence(s).

Mutagenesis Approaches for Affinity Maturation

As described above, antibody leads can be identified through a selectionprocess that involves screening the antibodies of a library of theinvention for binding to one or more antigens, or for a biologicalactivity. Coding sequences of these antibody leads may be furthermutagenized in vitro or in vivo to generate secondary libraries withdiversity introduced in the context of the initial antibody leads. Suchmutagenized antibody leads can then be further screened for binding totarget antigens or biological activity, in vitro or in vivo, followingprocedures similar to those used for the selection of the initialantibody lead from the primary library. Such mutagenesis and selectionof primary antibody leads effectively mimics the affinity maturationprocess naturally occurring in a mammal that produces antibodies withprogressive increases in the affinity to an antigen.

In some embodiments of the invention, only the CDRH3 region ismutagenized. In some embodiments of the invention, the whole variableregion is mutagenized. In some embodiments of the invention one or moreof CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and/CDRL3 may be mutagenized. Insome embodiments of the invention, “light chain shuffling” may be usedas part of the affinity maturation protocol. In certain embodiments,this may involve pairing one or more heavy chains with a number of lightchains, to select light chains that enhance the affinity and/orbiological activity of an antibody. In certain embodiments of theinvention, the number of light chains to which the one or more heavychains can be paired is at least about 2, 5, 10, 100, 10³, 10⁴, 10⁵,10⁶, 10⁷, 10⁸, 10⁹, or 10¹⁰. In certain embodiments of the invention,these light chains are encoded by plasmids. In some embodiments of theinvention, the light chains may be integrated into the genome of thehost cell.

Coding sequences of antibody leads may be mutagenized using any of widevariety of methods. Examples of methods of mutagenesis include, but arenot limited to site-directed mutagenesis, error-prone PCR mutagenesis,cassette mutagenesis, and random PCR mutagenesis. Alternatively oradditionally, oligonucleotides encoding regions with the desiredmutations can be synthesized and introduced into the sequence to bemutagenized, for example, via recombination or ligation.

Site-directed mutagenesis or point mutagenesis may be used to graduallychange the CDR sequences in specific regions. For example, this may beaccomplished by using oligonucleotide-directed mutagenesis or PCR. Forexample, a short sequence of an antibody lead may be replaced with asynthetically mutagenized oligonucleotide in either the heavy chain orlight chain region, or both. Such a method may not be efficient formutagenizing large numbers of CDR sequences, but may be used for finetuning of a particular lead to achieve higher affinity toward a specifictarget protein.

Cassette mutagenesis may alternatively or additionally be used tomutagenize the CDR sequences in specific regions. In a typical cassettemutagenesis, a sequence block, or a region, of a single template isreplaced by a completely or partially randomized sequence. However, themaximum information content that can be obtained may be statisticallylimited by the number of random sequences of the oligonucleotides.Similar to point mutagenesis, this method may also be used for finetuning of a particular lead to achieve higher affinity towards aspecific target protein.

Error-prone PCR, or “poison” PCR, may be used to mutagenize the CDRsequences, for example, by following protocols described in U.S. Pat.No. 6,153,745; Caldwell and Joyce, PCR Methods and Applications, 1992,2: 28; Leung et al., Technique, 1989, 1: 11; Shafikhani et al.,Biotechniques, 1997, 23: 304; and Stemmer et al., PNAS, 1994, 91: 10747(each of which is incorporated by reference in its entirety).

Conditions for error prone PCR may include, for example, (a) highconcentrations of Mn² (e.g., about 0.4 to about 0.6 mM) that efficientlyinduces malfunction of Taq DNA polymerase; and/or (b) adisproportionally high concentration of one nucleotide substrate (e.g.,dGTP) in the PCR reaction that causes incorrect incorporation of thishigh concentration substrate into the template and produces mutations.Alternatively or additionally, other factors such as, the number of PCRcycles, the species of DNA polymerase used, and the length of thetemplate, may affect the rate of misincorporation of “wrong” nucleotidesinto the PCR product. Commercially available kits may be utilized forthe mutagenesis of the selected antibody library, such as the “DiversityPCR random mutagenesis kit” (CLONTECH™).

Primer pairs used in PCR-based mutagenesis may, in certain embodiments,include regions matched with the homologous recombination sites in theexpression vectors. Such a design allows facile re-introduction of thePCR products back into the heavy or light chain chassis vectors, aftermutagenesis, via homologous recombination.

Other PCR-based mutagenesis methods can also be used, alone or inconjunction with the error prone PCR described above. For example, thePCR amplified CDR segments may be digested with DNase to create nicks inthe double stranded DNA. These nicks can be expanded into gaps by otherexonucleases such as Bal 31. Gaps may then be filled by random sequencesby using DNA Klenow polymerase at a low concentration of regularsubstrates dGTP, dATP, dTTP, and dCTP with one substrate (e.g., dGTP) ata disproportionately high concentration. This fill-in reaction shouldproduce high frequency mutations in the filled gap regions. Such methodsof DNase digestion may be used in conjunction with error prone PCR tocreate a high frequency of mutations in the desired CDR segments.

CDR or antibody segments amplified from the primary antibody leads mayalso be mutagenized in vivo by exploiting the inherent ability ofmutation in pre-B cells. The Ig genes in pre-B cells are specificallysusceptible to a high-rate of mutation. The Ig promoter and enhancerfacilitate such high rate mutations in a pre-B cell environment whilethe pre-B cells proliferate. Accordingly, CDR gene segments may becloned into a mammalian expression vector that contains a human Igenhancer and promoter. Such a construct may be introduced into a pre-Bcell line, such as 38B9, which allows the mutation of the VH and VL genesegments naturally in the pre-B cells (Liu and Van Ness, Mol. Immunol.,1999, 36: 461, incorporated by reference in its entirety). Themutagenized CDR segments can be amplified from the cultured pre-B cellline and re-introduced back into the chassis-containing vector(s) via,for example, homologous recombination.

In some embodiments, a CDR “hit” isolated from screening the library canbe re-synthesized, for example using degenerate codons ortrinucleotides, and re-cloned into the heavy or light chain vector usinggap repair.

Other Variants of Polynucleotide Sequences of the Invention

In certain embodiments, the invention provides a polynucleotide thathybridizes with a polynucleotide taught herein, or that hybridizes withthe complement of a polynucleotide taught herein. For example, anisolated polynucleotide that remains hybridized after hybridization andwashing under low, medium, or high stringency conditions to apolynucleotide taught herein or the complement of a polynucleotidetaught herein is encompassed by the present invention.

Exemplary low stringency conditions include hybridization with a buffersolution of about 30% to about 35% formamide, about 1 M NaCl, about 1%SDS (sodium dodecyl sulphate) at about 37° C., and a wash in about 1× toabout 2×SSC (20×SSC=3.0 M NaCl/0.3 M trisodium citrate) at about 50° C.to about 55° C.

Exemplary moderate stringency conditions include hybridization in about40% to about 45% formamide, about 1 M NaCl, about 1% SDS at about 37°C., and a wash in about 0.5× to about 1×SSC at abut 55° C. to about 60°C.

Exemplary high stringency conditions include hybridization in about 50%formamide, about 1 M NaCl, about 1% SDS at about 37° C., and a wash inabout 0.1×SSC at about 60° C. to about 65° C.

Optionally, wash buffers may comprise about 0.1% to about 1% SDS.

The duration of hybridization is generally less than about 24 hours,usually about 4 to about 12 hours.

Sublibraries and Larger Libraries Comprising Libraries or Sub-Librariesof the Invention

Libraries comprising combinations of the libraries described herein(e.g., CDRH3 and CDRL3 libraries) are encompassed by the invention.Sublibraries comprising portions of the libraries described herein arealso encompassed by the invention (e.g., a CDRH3 library in a particularheavy chain chassis or a sub-set of the CDRH3 libraries, for examplebased on length).

Moreover, libraries containing one of the libraries or sublibraries ofthe invention also fall within the scope of the invention. For example,in certain embodiments of the invention, one or more libraries orsublibraries of the invention may be contained within a larger library(theoretical or physical), which may include sequences derived by othermeans, for example, non-human or human sequence derived by stochastic orsitewise-stochastic synthesis. In certain embodiments of the invention,at least about 1% of the sequences in a polynucleotide library may bethose of the invention (e.g., CDRH3 sequences, CDRL3 sequences, VHsequences, VL sequences), regardless of the composition of the other 99%of sequences. For the purposes of illustration only, one of ordinaryskill in the art would readily recognize that a library containing 10⁹total members, where 10⁷ members are members of the libraries of theinvention (i.e., 1%) would have utility, and that members of thelibraries of the invention could be isolated from such a library. Insome embodiments of the invention, at least about 0.001%, 0.01%, 0.1%,2%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 81%, 82%, 83%, 84%, 85%,86%, 87%, 88%, 89%, 90%, 91,%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%of the sequences in any polynucleotide library may be those of theinvention, regardless of the composition of the other sequences. In someembodiments, the sequences of the invention may comprise about 0.001% toabout 1%, about 1% to about 2%, about 2% to about 5%, about 5% to about10%, about 10% to about 15%, about 15% to about 20%, about 20% to about25%, about 25% to about 30%, about 30% to about 35%, about 35% to about40%, about 40% to about 45%, about 45% to about 50%, about 50% to about55%, about 55% to about 60%, about 60% to about 65%, about 65% to about70%, about 70% to about 75%, about 75% to about 80%, about 80% to about85%, about 85% to about 90%, about 90% to about 95%, or about 95% toabout 99% of the sequences in any polynucleotide library, regardless ofthe composition of the other sequences. Thus, libraries more diversethan one or more libraries or sublibraries of the invention, but yetstill comprising one or more libraries or sublibraries of the invention,in an amount in which the one or more libraries or sublibraries of theinvention can be effectively screened and from which sequences encodedby the one or more libraries or sublibraries of the invention can beisolated, also fall within the scope of the invention.

Alternative Scaffolds

As would be evident to one of ordinary skill in the art, the CDRH3and/or CDRL3 polypeptides provided by the invention may also bedisplayed on alternative scaffolds. Several such scaffolds have beenshown to yield molecules with specificities and affinities that rivalthose of antibodies. Exemplary alternative scaffolds include thosederived from fibronectin (e.g., AdNectin), the β-sandwich (e.g., iMab),lipocalin (e.g., Anticalin), EETI-II/AGRP, BPTI/LACI-D1/ITI-D2 (e.g.,Kunitz domain), thioredoxin (e.g., peptide aptamer), protein A (e.g.,Affibody), ankyrin repeats (e.g., DARPin), γB-crystallin/ubiquitin(e.g., Affilin), CTLD₃ (e.g., Tetranectin), and (LDLR-A module)₃ (e.g.,Avimers). Additional information on alternative scaffolds is provided,for example, in Binz et al., Nat. Biotechnol., 2005 23: 1257 and Skerra,Current Opin. in Biotech., 2007 18: 295-304, each of which isincorporated by reference in its entirety.

Additional Embodiments of the Invention

Library Sizes

In some embodiments of the invention, a library comprises about 10¹ toabout 10²⁰ different polynucleotide or polypeptide sequences (encodingor comprising e.g., antibodies, heavy chains, CDRH3s, light chains,and/or CDRL3s). In some embodiments, the libraries of the invention aredesigned to include at least about 10¹, 10², 10³, 10⁴, 10⁵, 10⁶, 10⁷,10⁸, 10⁹, 10¹⁰, 10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵, 10¹⁶, 10¹⁷, 10¹⁸, 10¹⁹, or10²⁰, or more different antibody, heavy chain, CDRH3, light chain,and/or CDRL3 polynucleotide or polypeptide sequences. In someembodiments, a library of the invention may contain less than aparticular number of polynucleotide or polypeptide sequences, where thenumber of sequences is defined using any one of the integers providedabove. In certain embodiments of the invention, a particular numericalrange is defined, using any two of the integers provided above as lowerand upper boundaries of the range, inclusive or exclusive. Allcombinations of the integers provided, which define an upper and lowerboundary, are contemplated.

In some embodiments, the invention provides libraries wherein a fractionof the members of the library are members produced according to themethods, systems, and compositions provided herein. One importantproperty of the libraries of the invention is that they favorably mimiccertain aspects of the human preimmune repertoire, including lengthdiversity and sequence diversity. One or ordinary skill in the art willreadily recognize that libraries provided by the invention includelibraries where a subset of the members of the library are membersproduced according to the methods, systems, and compositions providedherein. For example, a library containing 10⁸ members wherein 10⁶members are produced according to the methods, systems, and compositionsprovided herein, would contain 1% sequences produced according to themethods, systems, and compositions provided herein. One of ordinaryskill in the art would recognize that one or more of the 10⁶ memberscould readily be isolated using screening techniques known in the art.Therefore, said libraries fall within the scope of the invention. Morespecifically, libraries comprising at least about 1%, 2.5%, 5%, 10%,15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%,90%, 95%, or 99% CDRH3, CDRL3, light chain, or heavy chain, and/orfull-length antibody sequences provided herein fall within the scope ofthe invention. Libraries comprising at least about 10³, 10⁴, 10⁵, 10⁶,10⁷, 10⁸, 10⁹, 10¹⁰, 10¹¹, 10¹², 10¹³, 10¹⁴, 10¹⁵ CDRH3, CDRL3, lightchain, heavy chain, and/or full-length antibody sequences providedherein also fall within the scope of the invention.

Human Preimmune Set

In some embodiments, the invention comprises the set of 3,571 curatedhuman preimmune antibody sequences contained within the HPS, theircorresponding CDRH3 sequences (Appendix A), and/or a representation ofthese CDRH3 sequences (and/or TN1, DH, N2, and/or H3-JH segmentsthereof) in a computer readable format. In certain embodiments, theinvention comprises a method of producing a CDRH3 library, the methodcomprising matching candidate segments (i.e., TN1, DH, N2, and H3-JH)from a theoretical segment pool with CDRH3 sequences in the HPS and/orany other repertoire of CDRH3 sequences. In some embodiments, theinvention comprises the candidate segments from the theoretical segmentpools disclosed herein and/or the segments selected for inclusion in aphysical library.

Embodiments

While the methods described herein demonstrate the production oftheoretical segment pools of H3-JH and DH segments using a limitednumber of allelic variants, one of ordinary skill in the art willrecognize that methods taught herein may be applied to any IGHJ and IGHDgenes, including any other allelic variants and all non-human IGHJ andIGHD genes. Alternatively or additionally, methods described herein maybe applied to any reference set of CDRH3 sequences, for example toextract additional TN1 and/or N2 segments. Alternatively oradditionally, one of ordinary skill in the art will recognize that eachof the described embodiments of the invention may be in polynucleotideor polypeptide form, within a vector, virus, or microorganism (e.g., ayeast or bacteria). Furthermore, since the invention involves syntheticlibraries that are fully enumerated, certain embodiments of theinvention relate to any of the embodiments described above in a computerreadable format, and uses thereof.

Non-human antibody libraries also fall within the scope of theinvention.

The present disclosure describes the removal of sequences containing Cysresidues, N-linked glycosylation motifs, deamidation motifs, and highlyhydrophobic sequences from the libraries of the invention. One ofordinary skill in the art will recognize that one or more of thesecriteria (i.e., not necessarily all) can be applied to removeundesirable sequences from any library of the invention. However,libraries containing one or more of these types of sequences also fallwithin the scope of the invention. Other criteria can also be used;those described herein are not limiting.

In certain embodiments, the invention provides libraries in which thenumber of times a particular sequence is repeated within the library(either theoretical, synthetic, or physical realization) is limited. Forexample, in some embodiments, the invention provides libraries whereinthe frequency of occurrence of any of the sequences in the library(e.g., CDRH3, CDRL3, heavy chain, light chain, full-length antibody) isless than about 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 95, 100, 200, 300, 400, 500, 600, 700, 800, 900, or1000. In some embodiments, the frequency of occurrence of any of thesequences in the library is less than a multiple of the frequency ofoccurrence of any other sequence in the library, for examples less thanabout 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80,85, 90, 95, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000 timesthe frequency of occurrence of any other sequence in the library.

In some embodiments, libraries are defined by the combinatorialdiversity of the segments used to produce CDRH3 sequences, in particularthe number of non-degenerate segment combinations that can be used toproduce a particular CDRH3 sequence. In some embodiments, this metricmay be calculated using, for example, a sample of about 2000, 5000,10000, 20000, 50000, 100000, or more sequences from the CDRH3 libraryand “self-matching” using the segments used to generate the CDRH3sequences of that library. In certain embodiments, the inventionprovides libraries wherein at least about 95%, 90%, 85%, 80%, 75%, 70%,65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10%, or 5% of theCDRH3 sequences in the library may be formed by a single combination ofsegments.

In certain embodiments of the invention, a statistical bootstrapanalysis was used to generate CDRH3 reference sets. While it may beadvantageous to use this method, it is not required for every embodimentof the invention.

In some embodiments, the invention provides methods and systems ofselecting polynucleotides to encode polypeptides of the invention,comprising selecting polynucleotide segments lacking (or containing)certain restriction sites individually and/or after combinatorialconcatenation with other segments (e.g., see Example 9.3.7).

The exemplary libraries provided herein are not limiting and providedfor exemplification only.

EXAMPLES

This invention is further illustrated by the following examples whichshould not be construed as limiting. The contents of all references,patents and published patent applications cited throughout thisapplication are hereby incorporated by reference.

In general, the practice of the present invention employs, unlessotherwise indicated, conventional techniques of chemistry, molecularbiology, recombinant DNA technology, PCR technology, immunology(especially, e.g., antibody technology), expression systems (e.g., yeastexpression, cell-free expression, phage display, ribosome display, andPROFUSION™), and any necessary cell culture that are within the skill ofthe art and are explained in the literature. See, e.g., Sambrook,Fritsch and Maniatis, Molecular Cloning Cold Spring Harbor LaboratoryPress (1989); DNA Cloning, Vols. 1 and 2, (D. N. Glover, Ed. 1985);Oligonucleotide Synthesis (M. J. Gait, Ed. 1984); PCR Handbook CurrentProtocols in Nucleic Acid Chemistry, Beaucage, Ed. John Wiley & Sons(1999) (Editor); Oxford Handbook of Nucleic Acid Structure, Neidle, Ed.,Oxford Univ Press (1999); PCR Protocols: A Guide to Methods andApplications, Innis et al., Academic Press (1990); PCR EssentialTechniques: Essential Techniques, Burke, Ed., John Wiley & Son Ltd(1996); The PCR Technique: RT-PCR, Siebert, Ed., Eaton Pub. Co. (1998);Antibody Engineering Protocols (Methods in Molecular Biology), 510,Paul, S., Humana Pr (1996); Antibody Engineering: A Practical Approach(Practical Approach Series, 169), McCafferty, Ed., Irl Pr (1996);Antibodies: A Laboratory Manual, Harlow et al., C.S.H.L. Press, Pub.(1999); Current Protocols in Molecular Biology, eds. Ausubel et al.,John Wiley & Sons (1992); Large-Scale Mammalian Cell Culture Technology,Lubiniecki, A., Ed., Marcel Dekker, Pub., (1990); Phage Display: ALaboratory Manual, C. Barbas (Ed.), CSHL Press, (2001); Antibody PhageDisplay, P O'Brien (Ed.), Humana Press (2001); Border et al., NatureBiotechnology, 1997, 15: 553; Border et al., Methods Enzymol., 2000,328: 430; ribosome display as described by Pluckthun et al. in U.S. Pat.No. 6,348,315, and Profusion™ as described by Szostak et al. in U.S.Pat. Nos. 6,258,558; 6,261,804; and 6,214,553; and bacterial periplasmicexpression as described in US20040058403A1. Each of the references citedin this paragraph is incorporated by reference in its entirety.

Further details regarding antibody sequence analysis using Kabatconventions and programs to analyze aligned nucleotide and amino acidsequences may be found, e.g., in Johnson et al., Methods Mol. Biol.,2004, 248: 11; Johnson et al., Int. Immunol., 1998, 10: 1801; Johnson etal., Methods Mol. Biol., 1995, 51: 1; Wu et al., Proteins, 1993, 16: 1;and Martin, Proteins, 1996, 25: 130. Each of the references cited inthis paragraph is incorporated by reference in its entirety.

Further details regarding antibody sequence analysis using Chothiaconventions may be found, e.g., in Chothia et al., J. Mol. Biol., 1998,278: 457; Morea et al., Biophys. Chem., 1997, 68: 9; Morea et al., J.Mol. Biol., 1998, 275: 269; Al-Lazikani et al., J. Mol. Biol., 1997,273: 927. Bane et al., Nat. Struct. Biol., 1994, 1: 915; Chothia et al.,J. Mol. Biol., 1992, 227: 799; Chothia et al., Nature, 1989, 342: 877;and Chothia et al., J. Mol. Biol., 1987, 196: 901. Further analysis ofCDRH3 conformation may be found in Shirai et al., FEBS Lett., 1999, 455:188 and Shirai et al., FEBS Lett., 1996, 399: 1. Further detailsregarding Chothia analysis are described, for example, in Chothia etal., Cold Spring Harb. Symp. Quant Biol., 1987, 52: 399. Each of thereferences cited in this paragraph is incorporated by reference in itsentirety.

Further details regarding CDR contact considerations are described, forexample, in MacCallum et al., J. Mol. Biol., 1996, 262: 732,incorporated by reference in its entirety.

Further details regarding the antibody sequences and databases referredto herein are found, e.g., in Tomlinson et al., J. Mol. Biol., 1992,227: 776, VBASE2 (Retter et al., Nucleic Acids Res., 2005, 33: D671);BLAST (www.ncbi.nlm.nih.gov/BLAST/); CDHIT(bioinformatics.ljcrf.edu/cd-hi/); EMBOSS(www.hgmp.mrc.ac.uk/Software/EMBOSS/); PHYLIP(evolution.genetics.washington.edu/phylip.html); and FASTA(fasta.bioch.virginia.edu). Each of the references cited in thisparagraph is incorporated by reference in its entirety.

Light Chain Libraries

Example 1 Light Chain Libraries with Framework and/or CDRL1 and/or CDRL2Variability

Although the diversity in antibody sequences is concentrated in theCDRs, certain residues in the framework regions can also influenceantigen recognition and/or modulate affinity (Queen et al., Proc. Natl.Acad. Sci. USA, 1989, 86: 10029; Carter et al., Proc. Natl. Acad. Sci.USA, 1992, 89: 4285, each incorporated by reference in its entirety).These residues have been cataloged and used to make frameworksubstitutions that improve antibody affinity, for example, during theprocess of antibody humanization (e.g., see the “Vernier” residues inFoote and Winter, J. Mol. Biol., 1992, 224: 487, incorporated byreference in its entirety). In the heavy chain, the Vernier residuesinclude Kabat-numbered residues 2, 27-30, 47-49, 67, 69, 71, 73, 78,93-94, and 103. In the light chain, the Vernier residues include Kabatresidues 2, 4, 35-36, 46-49, 64, 66, 68-69, 71, and 98. The Vernierresidue numbers are the same for kappa and lambda light chain sequences(see Table 4 in Chothia et al., J. Mol. Biol., 1985, 186: 651, which isincorporated by reference in its entirety). Additionally, frameworkpositions at the VL-VH interface may also influence affinity. In theheavy chain, the interface residues include Kabat residues 35, 37, 39,45, 47, 91, 93, 95, 100, and 103 (Chothia et al., J. Mol. Biol., 1985,186: 651, incorporated by reference in its entirety). In the lightchain, the interface residues include Kabat residues 34, 36, 38 44, 46,87, 89, 91, 96, and 98.

The following procedure was used to select the framework residues to bevaried and the amino acids to which they should be varied:

-   -   a. A collection of human VK light chain DNA sequences was        obtained from NCBI (see Appendix A of WO/2009/036379 for GI        Nos.). These sequences were classified according to the germline        origin of their VK germline segment.    -   b. Patterns of variation at each of the Vernier and interface        positions were examined as follows:        -   i. Equation 1 (from Makowski & Soares, Bioinformatics, 2003,            19: 483, incorporated by reference in its entirety) was used            to calculate a diversity index for the Vernier positions,            interface positions, CDRL1, and CDRL2.

$\begin{matrix}{d = \frac{1}{N{\sum p_{i\;}^{2}}}} & {{Equation}\mspace{14mu} 1}\end{matrix}$

-   -   -   Here, d is the diversity index, N is 20, the total number of            amino acid types, and p_(i) is the fraction of amino acid of            type “i” at the position of interest. The sum is carried out            over the 20 amino acid types. The parameter d will attain            its minimum value of 0.05 or 1/20, when a single amino acid            type is observed at a given position: p_(i) is 1 for one            type and zero for all the rest. Conversely, when all the            amino acid types are equally probable (e.g., p_(i) is 0.05            for all i), d will attain its maximum value of 1.0.        -   ii. The diversity index for each of the Vernier and            interface positions were compared to the diversity index for            the positions in CDRL1 and CDRL2.        -   iii. The interface positions were found to be relatively            invariant, with d values very close to the minimum of 0.05,            and were thus not altered. The Vernier residues with a            diversity index comparable to or larger than that of the CDR            positions (i.e., at or above 0.07 for the particular example            provided in FIG. 1) were selected as candidates for variance            (see FIG. 1). The amino acid residues included in these            positions were selected from amongst the two to three amino            acids most frequently occurring in that position in the            sequences in the collection of human VK light chains, for            each particular VK germline.        -   iv. Table 2 shows the positions selected for variance in            each of nine exemplified light chain germlines. The            alternative framework positions represent positions with a            diversity index less than the primary framework positions,            but where variability may still be incorporated to influence            antigen binding.        -   v. The amino acid residues in the framework positions            selected for variance were varied as follows (Table 3            provides the polypeptide sequences of these variants):            -   1. Position 2: Germline I was optionally changed to V.            -   2. Position 4: Germline M or L was optionally changed to                L or M. In some embodiments, changes from M to L, but                not the reverse, may be preferred, because M may undergo                oxidation during production, processing, or storage,                potentially altering the properties of the antibody.            -   3. Position 36: Germline Y was optionally changed to F                and H.            -   4. Position 46: Germline L was optionally changed to V.            -   5. Position 48: Germline I was optionally changed to L.            -   6. Position 49: Germline Y was optionally changed to S,                F, and H.            -   7. Position 66: Germline G was optionally changed to R                and E.

One of ordinary skill in the art would readily recognize that theprocedure outlined above could also be used to select positions to varyin Vλ germline sequences, and that libraries containing Vλ chains alsofall within the scope of the invention.

In addition to the framework mutations, variability was also introducedinto CDRL1 and CDRL2. This was performed by determining which residuesin CDRL1 and CDRL2 were variable, within a particular germline, in theVK dataset used above and incorporating the most frequently occurring 2to 4 variants into CDRL1 and CDRL2 in the synthetic libraries of theinvention. With the exception of position 50 of CDRL2 of the VK1-5germline, these alternatives did not arise from allelic variation. Table3 shows the polypeptide sequences of nine light chain chassis and theirframework and CDR L1/L2 variants for the currently exemplifiedembodiment of the invention. The amino acid residues in the CDRL1/L2positions selected for variance were varied as follows (using theChothia-Lesk numbering system; Chothia and Lesk, J. Mol. Biol., 1987,196: 901):

-   -   1. Position 28: Germline S or G were optionally changed to G, A,        or D.    -   2. Position 29: Germline V was optionally changed to I.    -   3. Position 30: Germline S was optionally changed to N, D, G, T,        A, or R.    -   4. Position 30A: Germline H was optionally changed to Y    -   5. Position 30B: Germline S was optionally changed to R or T.    -   6. Position 30E: Germline Y was optionally changed to N.    -   7. Position 31: Germline S was optionally changed to D, R, I, N,        or T.    -   8. Position 32: Germline Y or N were optionally changed to F, S,        or D.    -   9. Position 50: Germline A, D, or G were optionally changed to        G, S, E, K, or D.    -   10. Position 51: Germline G or A were optionally changed to A,        S, or T.    -   11. Position 53: Germline S or N were optionally changed to N,        H, S, K, or R.    -   12. Position 55: Germline E was optionally changed to A or Q.

Example 2 Light Chain Libraries with Enhanced Diversity in CDRL3

A variety of methods of producing light chain libraries are known in theart (e.g., see U.S. Publication Nos. 2009/0181855, 2010/0056386, andWO/2009/036379). An analysis of clinically validated antibody sequencesindicated that these sequences have very little deviation fromgermline-like VL-JL (where “L” can be a kappa or lambda germlinesequence) rearrangements prior to somatic mutation (FIG. 2). Here, agermline-like rearrangement is one where neither the V nor J portiondiffer from the respective germline genes and, for the purposes of thisparticular example, where the length of CDRL3 is restricted to 8, 9 or10 amino acids (see U.S. Publication Nos. 2009/0181855, 2010/0056386,and WO/2009/036379). For the IGHJK1 gene, however, both WT (Trp-Thr) andRT (Arg-Thr) sequences (the first two N-terminal residues) areconsidered “germline-like” and so are full L3 rearrangements containingsuch sequences. Therefore, new light chain libraries were designed andconstructed with the objectives of simultaneously (1) minimizingdeviation from germline-like sequences, as defined above; and (2)generating maximal diversity. In particular, the overarching goal was tomaximize the type of diversity that is indicated to be most favorable byclinically validated antibody sequences. In particular, the designedlibrary sought to maximize the diversity of CDRL3 sequences that differfrom length-matched germline sequences by two amino acids or fewer.

This was accomplished by utilizing a “jumping dimer” or “jumping trimer”approach to light chain oligonucleotide design. The jumping dimerapproach involves the incorporation of degenerate codons at each of thesix positions of CDRL3 encoded by the VL segment (L3-VL). At most twopositions vary from germline in each individual L3-VL sequence, but thetwo positions do not have to be adjacent to one another. Thus, the totalnumber of designed degenerate oligonucleotides synthesized per VLchassis is 6!/(4!2!), or fifteen (accounting for six of the mostcommonly occurring amino acids at the junction (position 96) between VLand JL for each kappa germline chassis (namely F, L, I, R, W, Y, and P;see U.S. Publication Nos. 2009/0181855 and 2010/0056386, andWO/2009/036379, each of which is incorporated by reference in itsentirety, for more details on the junctional amino acids at position96). The jumping trimer approach is analogous to the jumping dimerapproach, but with three positions varying from germline in eachindividual L3-VL sequence, instead of two as in the jumping dimer. Thedegenerate codons selected for each position in the jumping dimer andtrimer approaches were chosen to (1) to reproduce the diversitycontained in the known repertoire of publicly available human VKsequences (see Appendix A of WO/2009/036379); and (2) to minimize oreliminate undesirable sequences within the CDRL3s of the resultingsynthetic light chains, such as N-linked glycosylation motifs (NXS/NXT),Cys residues, stop codons, and deamidation-prone NG motifs. Table 4shows the fifteen degenerate oligonucleotides encoding the VK1-39 CDRL3sequences with a length of nine amino acids and F or Y as the junctionalamino acid, and the corresponding degenerate polypeptide sequences.Table 5, Table 6, and Table 7 provide the oligonucleotide sequences foreach of the VK sequences of the exemplary jumping dimer and trimerlibraries, for CDRL3 lengths of 8, 9, and 10, respectively, and thesequences for the corresponding CDRL3s.

The number of unique CDRL3 sequences within each germline library wasthen enumerated and compared to the number of unique CDRL3 sequences ina different light chain library, designated “VK-v1.0” (see Example 6.2in US Publication No. 2009/0181855), for each of the three lengths.Table 8 provides the number of unique CDRL3 sequences in each of therespective germline libraries.

FIG. 3 provides the percentage of sequences in the jumping dimer andVK-v1.0 libraries with CDRL3 length of nine amino acids that contain nomutations from germline-like sequences (Table 1) or 1, 2, 3, or 4 orfewer mutations from germline-like sequences. Naturally-occurring VK1-05sequences are almost as likely to have Ser (germline amino acid type) asPro at Kabat position 95, thus both residues (S and P) were incorporatedin the synthetic libraries representing VK1-05 repertoires. However, asindicated in Table 1, only Ser was considered to be a germline-likeresidue at position 95 for the purposes of this analysis when the VKgene is VK1-05. The plot for VK3-20 is representative of the remainingchassis in the library for a length nine. All of the sequences in theVK1-05 library were within three amino acids of human germlinesequences, and approximately 63% of the sequences were within two aminoacids of human germline-like sequences. For the rest of the libraries,and as designed, 100% of the sequences were within two amino acids ofhuman germline-like sequences; thus, over 95% of the sequences of length9 in the jumping dimer library considered as a whole were within 2 aminoacids of germline-like sequences. By comparison, only 16% of the membersof the VK-v1.0 libraries of length nine amino acids are within two aminoacids of the corresponding human germline-like sequences. For length 8,about 98% of the sequences in the jumping dimer libraries were withintwo amino acids of germline-like, versus about 19% for VK-v1.0. Forlength 10, more than 95% of the sequences of the jumping dimer librarywere within two amino acids of germline-like, versus about 8% forVK-v1.0.

In some embodiments, to concentrate the diversity in positions mostlikely to be solvent-exposed in the folded antibody, positions 89 and 90(Kabat numbering) are not modified from germline—these are most oftenQQ, but the sequence is MQ for the VK2-28 chassis. Other VK germlinegenes have different sequences at positions 88-89, and the use of thesegenes as chassis also falls within the scope of the invention. Forexample, VK1-27 has QK, VK1-17 and VK1-6 both have LQ, and so on. Thesequences in these positions are known in the art and can be obtained,for example, from Scaviner et al., Exp. Clin. Immunogenet., 1999, 16:234 (see FIG. 2), which is incorporated by reference in its entirety.

CDRH3 Libraries

The following examples describe methods and compositions useful for thedesign and synthesis of antibody libraries with improved CDRH3 sequencesin comparison to libraries known in the art. The CDRH3 sequences of theinvention have enhanced diversity in comparison to libraries known inthe art, while retaining the character of human sequences, improvingcombinatorial efficiency of the synthetic CDRH3 segments, and/orimproving the matching between synthetic CDRH3 sequences and human CDRH3sequences in one or more reference sets.

Example 3 Generating a Curated Reference Set of Human Preimmune CDRH3Sequences

A file containing approximately 84,000 human and mouse heavy chain DNAsequences was downloaded from the BLAST public resource(ftp.ncbi.nih.gov/blast/db/FASTA/; filename: igSeqNt.gz; download date:Aug. 29, 2008). Of these approximately 84,000 sequences, approximately34,000 sequences were identified as human heavy chain sequences based onanalysis of the sequence header annotation. These sequences were thenfiltered as follows: First, all sequences were classified, via theirVH-region, according to their corresponding (closest matched) VHgermline. Sequences that were of an incorrect or insufficient length, orthat could not be matched due to extensive mutation, were discarded.Second, any sequences containing more than five mutations, at the DNAlevel, when compared to their corresponding germline VH sequence werealso discarded. It was assumed, consistent with Rada and Milstein, EMBOJ., 2001, 20: 4570, that mutations (or lack thereof) in the N-terminalportion of the variable region may be used as conservative surrogatesfor mutations (or lack thereof) in the C-terminal portion of thevariable region, in particular in CDRH3. Therefore, selecting onlysequences with five or fewer nucleotide mutations in VH, which isN-terminal to CDRH3 is highly likely to also select for CDRH3 sequencesthat are either lightly mutated or not mutated at all (i.e., havingpreimmune character).

After translating the remaining DNA sequences into their amino acidcounterparts, the appropriate reading frame containing the heavy chaingermline amino acid sequence was identified and used to identify thesequences of the CDRs, including that of the CDRH3. The list of CDRH3sequences obtained at this point was further filtered to eliminatemembers that did not differ from any other sequence in the set by atleast three amino acids (after matching for length). This processyielded 11,411 CDRH3 sequences, with 3,571 sequences annotated asoriginating from healthy adults (“Healthy Preimmune Set” or “HPS”; seeAppendix A for GI Nos.) and the other 7,840 sequences annotated asoriginating from individuals suffering from disease, of fetal origin, orof antigen-specific origin. The methods described below were then usedto deconvolute each of the sequences in the HPS into the four segmentsthat constitute the CDRH3: (1) TN1, (2) DH, (3) N2, and (4) H3-JH.

Example 4 Method to Match Segments from a Theoretical Segment Pool toCDRH3s in a Reference Set

This example describes the method used to identify the TN1, DH, N2, andH3-JH segments of the CDRH3s in the HPS. The currently exemplifiedapproach to the design and synthesis of human CDRH3 sequences mimics thesegmental V-D-J gene recombination processes by which the human immunesystem generates the preimmune CDRH3 repertoire. The matching methoddescribed here determines which TN1, DH, N2 and H3-JH segments have beenused to produce a particular CDRH3 across a reference set of CDRH3s(e.g., the HPS). This information is then used, optionally inconjunction with other information described below (e.g.,physicochemical properties), to determine which TN1, DH, N2, and H3-JHsegments from a theoretical segment pool (or segments extracted from theCDRH3 sequences in the reference set, in the case of the TN1 and N2)should be included in a synthetic CDRH3 library.

The inputs to the matching method are: (1) a reference set of CDRH3sequences (e.g., the human CDRH3 sequences in the HPS), and (2) atheoretical segment pool, containing a plurality of TN1, DH, N2 and/orH3-JH segments. Methods by which the members of the theoretical segmentpool are generated are more fully described below. For each CDRH3 in thereference set, the matching method generates two outputs: (i) a list ofthe closest matched CDRH3 sequences that can be generated using thesegments of the theoretical segment pool, and (ii) the one or moresegment combinations from the theoretical segment pool that can be usedto create these closest matched CDRH3 sequences.

The matching method was performed as follows: Each TN1 segment in thetheoretical segment pool was aligned at its first amino acid with thefirst amino acid (position 95) of the CDRH3 sequence from the referenceset. For each segment length, all (i.e., one or more) of the segmentsreturning the best matches are retained, and the remaining segments arediscarded. The retained TN1 segments are then concatenated with all DHsegments from the theoretical segment pool, to create [TN1]-[DH]segments. These segments are then aligned as above, and all the bestmatches for each of the [TN1]-[DH] segments are retained. The procedureis repeated with [TN1]-[DH]-[N2] and [TN1]-[DH]-[N2]-[H3-JH] segmentsuntil the length of the CDRH3 sequence from the reference set isidentically recapitulated by the segment combinations from thetheoretical segment pool. All segment combinations returning the bestmatch to the CDRH3s in the reference set are retained as the output ofthe matching method.

Table 9 provides an example of the output of the matching method,specifically the output for four individual sequences from the HPS,using a theoretical segment pool designated “Theoretical Segment Pool1,” or “TSP1”. TSP1 contains several theoretical segment pools, namely:212 TN1 segments (Table 10), 1,111 DH segments (Table 11), 141 N2segments (Table 12), and 285 H3-JH segments (Table 13). The CDRH3sequence in Test Case 1 contains an identical match in TSP1 that isreached via a unique combination of the four segments. Test Cases 2.1and 2.2 each return an identical match, but via two distinctcombinations that differ in the TN1 and DH segments. In Test Cases 3.1,4.1, and 4.2, the closest matches are all a single amino acid away fromthe reference CDRH3, and can be reached via one (3.1) or two (4.1 and4.2) combinations of segments from TSP1. This approach can begeneralized to find all of the closest matches to any reference CDRH3sequence within any theoretical segment pool and all combinations of thesegments within the theoretical segment pool that can produce thereference CDRH3 sequence exactly and/or its closest matches.

Example 5 Deriving Theoretical Segment Pools of H3-JH Segments

In order to produce theoretical segment pools of H3-JH segments forconsideration for inclusion in a synthetic CDRH3 library, the followingmethod was applied to generate mutants of seven (IGHJ1-01, IGHJ2-01,IGHJ3-02, IGHJ4-02, IGHJ5-02, IGHJ6-02 and IGHJ6-03) of the twelvegermline IGHJ sequences of Table 14. These seven alleles were chosenbecause they were among the most commonly occurring alleles in humansequences. Libraries where all sequences of Table 14 (some differingonly in FRM4) are used to generate H3-JH and/or JH (i.e., H3-JH andFRM4) also fall within the scope of the invention. The method isintended to simulate the creation of junctional diversity during theV-D-J recombination process in vivo, which occurs via enzyme-mediatedaddition and deletion of nucleotides to the germline gene segments. Themethod proceeds as follows, and results in a fully enumeratedtheoretical segment pool of H3-JH segments:

-   -   1. A pre-treatment was applied to the IGHJ genes that contain a        partial codon consisting of two nucleotide bases at their 5′        terminus (IGHJ3-02, IGHJ4-02, IGHJ5-02, IGHJ6-02 and IGHJ6-03),        prior to the first nucleotide encoding the translation of the JH        segment that produces the well-known JH framework regions. For        example, the IGHJ3-02 gene contains an AT dinucleotide sequence        prior to the first nucleotide encoding the translation of the JH        segment that produces the JH framework region (FIG. 4, top). All        partial codons consisting of two nucleotide bases were        completed, using all possible nucleotide doublets (i.e., NN) at        their two most 5′ positions (FIG. 4, top, second row for        IGHJ3-02). More specifically, the most 5′ nucleotide in the        germline sequence was mutated to N and an additional N was added        5′ to that nucleotide.    -   2. IGHJ genes IGHJ1-01 (FIG. 4, center) and IGHJ2-01 (FIG. 4,        bottom) contain zero and one nucleotide base(s) at their 5′        termini, prior to the first nucleotide encoding the translation        of the JH segment that produces the JH framework region. For        these genes, the pre-treatment described in step 1 was not        performed. Instead, the 5′ doublets were mutated to NN (FIG. 4,        middle and bottom, second row of each). Therefore, after        performing this step, each of the seven IGHJ genes enumerated        above was converted to a variant with an NN doublet as its first        two 5′ positions.    -   3. The 5′ codons of the sequences produced via steps 1 and 2        were then deleted, and the first two bases of the resulting DNA        sequence were subsequently mutated to an NN doublet (FIG. 4,        rows 3-4 for all).    -   4. The 5′ codons of the sequences produced in step 3 were then        deleted, and the first two bases of the resulting DNA sequence        were subsequently mutated to an NN doublet (FIG. 4, rows 5-6 for        all).    -   5. Each of the polynucleotide sequences generated by steps        (1)-(4) were then translated, to obtain a theoretical segment        pool consisting of 248 parent H3-JH polypeptide segments        (Table 15) from the reading frame for each sequence that        produced the JH framework region.    -   6. The parent H3-JH polypeptide segments were truncated at their        N-termini, by removing one amino acid at a time until only the        portion of the JH segment comprising FW4 remains (i.e., an H3-JH        segment with a length of zero amino acids).

The methods described above resulted in the production of a theoreticalsegment pool of 285 H3-JH segments (Table 13).

Example 6 Deriving Theoretical Segment Pools of DH Segments

Two theoretical pools of DH segments were generated, using one or moreof two translation methods, designated “Translation Method 0” (TM0), or“Translation Method 1” (“TM1”), each performed in the three forwardreading frames of 27 human germline IGHD DNA sequences or segmentsderived therefrom (Table 16).

The 1K DH Theoretical Segment Pool (1K DH)

TM1 was used to generate the “1K DH Theoretical Segment Pool” (“1K DH”;see the 1,111 DH segments of Table 11). In TM1, IGHD sequences that hada partial codon containing two untranslated bases after translation inany of the three forward reading frames were completed to produce a fullcodon only if the two bases could encode only a single amino acid uponcompletion. For example, a DNA sequence such as TTA-GCT-CG has two fullcodons that would be translated to LA, and a remaining partial codon(CG) that can only encode R, as any of CGA, CGC, CGG, or CGT will encodeR. Thus, applying TM1 to this sequence will yield LAR. For sequenceswith partial codons that could encode more than one amino acid (e.g., GAor AG), the partial codons were ignored. Applying TM1 to the 27 IGHDsequences of Table 16 generated a theoretical segment pool containingthe 73 DH parent segments of Table 17 (some containing stop codons (“Z”)and unpaired Cys residues). These sequences were then progressivelydeleted at the amino acid level, at their N- and C-termini, until onlytwo amino acids remained. Truncated segments were discarded if theycontained a stop codon, unpaired Cys residues, N-linked glycosylationmotifs, or deamidation motifs. This process yielded the 1,111 DHsegments of Table 11.

The 68K DH Theoretical Segment Pool (68K DH)

The 27 IGHD genes and alleles of Table 16 were progressively deleted oneither or both of their 5′ and 3′ ends until four bases remained,yielding 5,076 unique polynucleotide sequences of four or morenucleotides. These 5,076 sequences were subjected to systematic additionof 0, 1 and/or 2 N nucleotides their 5′ and/or 3′ ends. The resultingsequences were translated using TM0. In TM0, only full codons aretranslated; partial codons (i.e., 1 or 2 bases) are ignored. This methodyielded 68,374 unique DH polypeptide segments after elimination ofsegments with stop codons, unpaired Cys residues, Asn in the last ornext to last position that can lead to N-linked glycosylation motifs,and deamidation motifs (the “68K DH Theoretical Segment Pool”). Usingthe IGHD genes of Table 16 as an input for the PYTHON computer codeprovided below will reproduce the exact theoretical segment pool of68,374 DH segments. There are two free parameters in this program: (1)the minimum length of the DNA sequences remaining after progressivedeletions (4 bases in this example), and (2) the minimum length of thepeptide sequences (2 amino acids in this example) acceptable forinclusion in the theoretical segment pool. These parameters can bechanged to alter the output of the program. For example, changing thefirst parameter to one base and the second parameter to one amino acidwould lead to a larger theoretical segment pool with 68,396 uniquesequences, including 18 single-amino acid segments. DH segmentsprogressively truncated to different lengths also fall within the scopeof the invention; for example those truncated to 1, 2, 3, or 4 or moreamino acids, or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more nucleotides priorto translation.

PYTHON Computer Program to Generate 68,374 DH Segments import math, sys,string class genes:   name = ′x′   seq = ′x′   progdel = set( ) classtable:  name = ′x′  dna = ′x′  dna_n = 20 * [′x′]  prot = 60 * [′x′] uprot = set( ) pepcod = {′A′:0, ′C′:1, ′D′:2, ′E′:3, ′F′:4, ′G′:5,′H′:6, ′I′:7, ′K′:8, ′L′:9, ′M′:10, ′N′:11, ′P′:12, ′Q′:13, ′R′:14,′S′:15, ′T′:16, ′V′:17, ′W′:18, ′Y′:19, ′Z′:20} codpep = 21 * [″]codpep[0] = ′A′ codpep[1] = ′C′ codpep[2] = ′D′ codpep[3] = ′E′codpep[4] = ′F′ codpep[5] = ′G′ codpep[6] = ′H′ codpep[7] = ′I′codpep[8] = ′K′ codpep[9] = ′L′ codpep[10] = ′M′ codpep[11] = ′N′codpep[12] = ′P′ codpep[13] = ′Q′ codpep[14] = ′R′ codpep[15] = ′S′codpep[16] = ′T′ codpep[17] = ′V′ codpep[18] = ′W′ codpep[19] = ′Y′ # Zrepresents a stop codon codpep[20] = ′Z′ bases = ′ACGT′ deftranslate_dna(sequence): # Translation of input DNA sequence usingstandard genetic code # Only full codons are considered with anyremaining 1 or 2 bp being ignored # Z represents a stop codon   code = {  ′ATA′:′I′, ′ATC′:′I′, ′ATT′:′I′, ′ATG′:′M′,   ′ACA′:′T′, ′ACC′:′T′,′ACG′:′T′, ′ACT′:′T′,   ′AAC′:′N′, ′AAT′:′N′, ′AAA′:′K′, ′AAG′:′K′,  ′AGC′:′S′, ′AGT′:′S′, ′AGA′:′R′, ′AGG′:′R′,   ′CTA′:′L′, ′CTC′:′L′,′CTG′:′L′, ′CTT′:′L′,   ′CCA′:′P′, ′CCC′:′P′, ′CCG′:′P′, ′CCT′:′P′,  ′CAC′:′H′, ′CAT′:′H′, ′CAA′:′Q′, ′CAG′:′Q′,   ′CGA′:′R′, ′CGC′:′R′,′CGG′:′R′, ′CGT′:′R′,   ′GTA′:′V′, ′GTC′:′V′, ′GTG′:′V′, ′GTT′:′V′,  ′GCA′:′A′, ′GCC′:′A′, ′GCG′:′A′, ′GCT′:′A′,   ′GAC′:′D′, ′GAT′:′D′,′GAA′:′E′, ′GAG′:′E′,   ′GGA′:′G′, ′GGC′:′G′, ′GGG′:′G′, ′GGT′:′G′,  ′TCA′:′S′, ′TCC′:′S′, ′TCG′:′S′, ′TCT′:′S′,   ′TTC′:′F′, ′TTT′:′F′,′TTA′:′L′, ′TTG′:′L′,   ′TAC′:′Y′, ′TAT′:′Y′, ′TAA′:′Z′, ′TAG′:′Z′,  ′TGC′:′C′, ′TGT′:′C′, ′TGA′:′Z′, ′TGG′:′W′,   }   proteinseq ″   for nin range(0, len(sequence), 3):    if code.has_key(sequence[n:n+3]) ==True:     proteinseq += code[sequence[n:n+3]]   return proteinseq # mainbody starts here # open input and output files in1 = open(sys.argv[1],′r′) ou1 = open(sys.argv[2], ′w′) # read DNA sequences for input DHsegments data = in1.readlines( ) nseg = len(data) seqs = [ genes( ) fori in range(nseg) ] for i in range(nseg):  line = data[i]  words =string.split(line)  seqs[i].name = words[0]  seqs[i].seq = words[1] seqs[i].progdel = set( ) # Define here minimum length for DNA (4) andfor protein (2) minlen = 4 minp = 2 # Implement progressive base by basedeletion from 5' or 3' or both ends alln = 0 for i in range(nseg):   seq= seqs[i].seq   lseq = len(seq)   nt = ct = lseq   for n in range(nt):   for c in range(ct):     nseq = seq[n:lseq−c]     if (len(nseq) >=minlen):      seqs[i].progdel.add(nseq)   alln += len(seqs[i].progdel) #Collect unique DNA sequences across all DH genes of origin and ignoreredundant ones progdel = [ table( ) for i in range(alln) ] n = 0 for iin range(nseg):   for kk in seqs[i].progdel:    unix = 1    for j inrange(n):     if (kk == progdel[j].dna):      unix = 0      break    if(unix == 1):     progdel[n].name = seqs[i].name     progdel[n].dna = kk    n +=1 # Add none, 1 or 2 bp on one or both ends # extras 20 + 20 *(21) = 20 + 420 = 440 # allocate memory for all variants for i inrange(n):    progdel[i].dna_n = 440 * [′x′]    progdel[i].prot = 441*3 * [′x′]    progdel[i].uprot = set( ) # add 1 or 2 bases at each endof input segment tot = 0 for i in range(n): # Step over each unique DNAsequence  k = 0 # One base on 5' end combined with 1 or 2 bases added to3' end  for 15 in range(4):   progdel[i].dna_n[k] = bases[15] +progdel[i].dna   k +=1   for 13 in range(4):    progdel[i].dna_n[k] =bases[15] + progdel[i].dna + bases[13]    k +=1   for 13 in range(4):   for m3 in range(4):     progdel[i].dna_n[k] = bases[15] +progdel[i].dna +   bases[13] + bases[m3]     k +=1 # One or two basesadded to 3' only in this part  for 13 in range(4):   progdel[i].dna_n[k]= progdel[i].dna + bases[13]   k +=1  for 13 in range(4):   for m3 inrange(4):    progdel[i].dna_n[k] = progdel[i].dna + bases[13] +   bases[m3]    k +=1 # Two bases on 5' end combined with 1 or 2 bp on3' end  for 15 in range(4):   for m5 in range(4):    progdel[i].dna_n[k]= bases[15] + bases[m5] +    progdel[i].dna    k +=1    for 13 inrange(4):     progdel[i].dna_n[k] = bases[15] + bases[m5] +progdel[i].dna + bases[13]     k +=1    for 13 in range(4):     for m3in range(4):     progdel[i].dna_n[k] = bases[15] + bases[m5] +progdel[i].dna + bases[13] + bases[m3]     k +=1 # Now translate in all3 forwared reading frames # Save unique peptide sequences  for fr inrange(3):   piece = progdel[i].dna   piece = piece[fr:]   tpiece =translate_dna(piece)   progdel[i].prot[fr] = tpiece  progdel[i].uprot.add(tpiece)  for k in range(440):   piece =progdel[i].dna_n[k]   piece = piece[fr:]   tpiece = translate_dna(piece)  progdel[i].uprot.add(tpiece)   progdel[i].prot[3+440*fr +k] = tpiece tot += len(progdel[i].uprot) # Collect unique sequences with no ASN atlast or next to last position, no unpaired or consecutive CYS, no stopsunset = set( ) segm = [ genes( ) for i in range(tot) ] lux = 0 nn = 0for i in range(n):   k = 0   for kk in progdel[i].uprot: # Filter outsequences with undesired features, including length being too short(under “minp” defined above)     if (len(kk) < minp): continue     if(kk[len(kk)−1] == “N” or kk[len(kk)−2] == “N”): continue     if(kk.count(“Z”) > 0 or kk.count(“CC”) >0 or kk.count(“C”) % 2 >0):continue     unset.add(kk)     lux1 = len(unset)     if (lux1 > lux):     segm[nn].name = progdel[i].name + “_” + str(nn)      segm[nn].seq =kk      nn += 1     lux = lux1     k +=1 # Print out unique peptidesequences that pass all the filters for i in range(nn):ou1.write(″%s\t%s\n″ % (segm[i].name, segm[i].seq))

Example 7 Deriving Theoretical Segment Pools of TN1 and N2 Segments

The libraries of this example are designed to, in some instances, have agreater diversity in their TN1 and N2 segments in comparison to otherlibraries known in the art. The diversity of the TN1 and N2 segments wasincreased by using the matching method described in Example 4 todeconvolute the CDRH3 sequences in the HPS into their constituentsegments (i.e., TN1, DH, N2, and H3-JH), followed by extraction of“novel” TN1 and N2 segments in the manner described below. For thepurposes of the invention, “novel” TN1 and N2 segments are TN1 and N2segments that do not appear in a theoretical segment pool that ismatched to a reference set of CDRH3 sequences. Following is an exampleof the method used to extract novel TN1 and N2 segments from the HPS.This method can be generalized to extract novel TN1 and N2 segments fromany reference set of CDRH3 sequences, using any theoretical segment poolcontaining TN1, DH, N2, and/or H3-JH segments.

Table 9 provides the matching results for the reference CDRH3 sequenceERTINWGWGVYAFDI (SEQ ID NO: 8760) (Test Cases 5.1-5.4) from the HPS,using Theoretical Segment Pool 1 (“TSP1”). The best matches to thereference CDRH3 are four CDRH3 sequences, each within three amino acidsof the reference CDRH3 sequence. In each of these matches, the TN1, DH,N2 and H3-JH segments are of length 4, 3, 3 and 5 amino acids,respectively. Thus the reference CDRH3 can be deconvoluted into thefollowing segments: ERTI-NWG-WGW-YAFDI (SEQ ID NO: 8761) (i.e.[TN1]-[DH]-[N2]-[H3-JH], respectively). The DH and H3-JH segments fromthe reference CDRH3, NWG and YAFDI (SEQ ID NO: 4540) respectively, areidentically present in TSP1. However, the TN1 (ERTI) (SEQ ID NO: 8718)and N2 (WGW) segments from the reference CDRH3 are absent in TSP1 andmatch TSP1 segments with one or more amino acid mismatches. These“novel”TN1 and N2 segments are extracted from the reference CDRH3 andconsidered for inclusion prospective theoretical segment pools and/orsynthetic libraries. Additional novel TN1 and N2 segments wereaccumulated by applying this analysis to all members of the HPS. Inorder to robustly identify TN1 and N2 sequences, the extraction wasperformed only for those CDRH3 sequences in which the DH and H3-JHsegments in the reference CDRH3 and TSP1 cumulatively return no morethan 3 amino acid mismatches, implying that the DH and H3-JH segments ofthe reference CDRH3 had been reliably assigned.

Example 8 Calculation of Segment Usage Weights

Segment usage weights were calculated for their utility in determiningwhich segments from the theoretical segment pools (e.g., TSP1 and TSP1plus novel TN1 and N2 segments identified as described in Example 7)should be included in a synthetic library. Segment usage weights wereobtained by utilization of the matching method described above andEquation 2:

$\begin{matrix}{{w(i)} = {\frac{1}{S_{m}}{\sum\limits_{j = 1}^{S_{m}}{\frac{1}{g(j)}{\sum\limits_{k = 1}^{g{(j)}}{f_{i}(k)}}}}}} & {{Equation}\mspace{14mu} 2}\end{matrix}$where,

-   -   w(i): Weight for segment i. 0≦w(i)≦1.    -   S_(m): Number of sequences (out of total Sin the reference CDRH3        set) which contain one or more best matches with no more than m        amino acid mismatches in the specified region of the reference        CDRH3 sequence. Here, the mismatches are computed over the        Kabat-CDRH3 region, but other fragments of the CDRH3 sequences        may also be considered. A constant value of m=3 was used here,        but other values may be used, or the value may depend on the        length of the reference CDRH3 sequence.    -   g(j): Total number of degenerate segment combinations producing        the best match to the reference CDRH3 sequence j.    -   f_(i) (k): Fractional amino acid identity of TN1, DH, N2 or        H3-JH segment in degenerate match k, relative to the        corresponding sequence fragment in the reference CDRH3        sequence j. The fractional amino acid identity equals zero if        the segment does not appear in match k. Other definitions off,        such as amino acid similarity (e.g., based on physicochemical        properties of the amino acids such as hydrophobicity), instead        of identity, may be also used.

The procedure for calculating segment usage weights will be furtherexemplified below. In each of these examples, the best matchcombinations from TSP1 are provided for a single CDRH3 sequence(S_(m)=1) and the degeneracy (k) and fractional mismatch (f) dependentweight calculations are explained.

Example 8.1 Segment usage weights for Test Case 1 in Table 9

Refer to Test Case 1 in Table 9. The CDRH3 sequence RTAHHFDY (SEQ ID NO:3660) is identically located in TSP1 (f =1, subscripts dropped forsimplicity) via a unique segment combination (g =1). Table 18 providesthe usage weights for the segments corresponding to the best match fromTSP1 for the CDRH3 of Test Case 1.

Example 8.2 Segment usage weights for Test Cases 2.1 and 2.2 in Table 9

Refer to Test Cases 2.1 and 2.2 in Table 9. The CDRH3 sequence VGIVGAASY(SEQ ID NO: 3661) may be identically located in TSP1 (f=1) via twodistinct segment combinations (g =2). Table 19 provides the usageweights for the segments corresponding to the best match from TSP1 forthe CDRH3 of Test Cases 2.1 and 2.2.

Example 8.3 Segment usage weights for Test Case 3.1 in Table 9

Refer to Test Case 3.1 in Table 9. The CDRH3 sequence DRYSGHDLGY (SEQ IDNO: 3662) may be identically located in TSP1 via a unique segmentcombination (g =1) with a single amino acid difference. As providedbelow, the TN1, N2 and H3-JH segments match the corresponding referencesequence fragments identically, while four of the five DH amino acidsmatch identically.

(SEQ ID NO: 3662) Sequence from the HPS: DR-YSGHD-LG-Y (SEQ ID NO: 8719)Nearest Neighbor in TSP1: DR-YSGYD-LG-YThus, here

$\begin{matrix}{{f = {4\text{/}5\mspace{14mu}{for}\mspace{14mu}{the}\mspace{14mu}{DH}\mspace{14mu}{segment}}};{and}} \\{{= {1\mspace{14mu}{for}\mspace{14mu}{the}\mspace{14mu}{TN}\; 1}},{N\; 2},\;{{and}\mspace{14mu} H\; 3\text{-}{JH}\mspace{14mu}{segments}\mspace{14mu}{\left( {{Table}\mspace{14mu} 20} \right).}}}\end{matrix}$

Example 8.4 Matching of Test Cases 4.1 and 4.2 in Table 9

Refer to test cases 4.1 and 4.2 in Table 9. The CDRH3 sequenceGIAAADSNWLDP (SEQ ID NO: 3663) may be located in TSP1 via two distinctsegment combinations (g=2), each with a single amino acid difference. Asprovided below, the TN1, DH and N2 segments match the correspondingreference sequence fragments identically, while five of the six H3-JHamino acids match identically.

(SEQ ID NO: 3663) Sequence from HPS: (−)-GIAAA-D-SNWLDP(SEQ ID NO: 8720) Nearest Neighbor in TSP1: (−)-GIAAA-D-SNWFDP(SEQ ID NO: 3663) Sequence from HPS: G-IAAA-D-SNWLDP (SEQ ID NO: 8720)Nearest Neighbor in TSP1: G-IAAA-D-SNWFDPHere, (−) represents the “empty” TN1 segment.Applying Equation 2 results in the segment usage weights provided inTable 21.

Example 8.5 Calculating the Segment Usage Weights for Test Cases 1 to4.2 of Table 9

Extending the individual calculations described above to simultaneouslyinclude all of Test Cases 1 to 4.2 of Table 9 results in the segmentusage weights of Table 22.

Example 8.6 Calculating the Segment Usage Weights for Test Cases 5.1 to5.4 of Table 9

Refer to the CDRH3 sequence ERTINWGWGVYAFDI (SEQ ID NO: 8760) and thenovel TN1 and N2 segments extracted from the CDRH3 sequence, in Example7. In this case, the novel TN1 and N2 segments (ERTI (SEQ ID NO: 8718)and WGV respectively), and the DH and H3-JH segments from TSP1 (NWG andYAFDI (SEQ ID NO: 4540) respectively) are each assigned usage weights ofunity.

Example 9 Selection of TN1, DH, N2 and JH Segments for Inclusion inSynthetic Libraries

FIG. 5 provides the general method used for the design of syntheticCDRH3 libraries. The method uses as input: (1) a theoretical segmentpool containing TN1, DH, N2, and H3-JH segments (e.g., TSP plus novelTN1 and N2 segments); and (2) a collection of reference CDRH3 sequences(e.g., the HPS). From these inputs, a particular subset of segments fromthe theoretical segment pool is selected for inclusion in a physicalCDRH3 library.

First, the best matches to the CDRH3s of the HPS were obtained, fromwithin the TSP1 set, with or without the novel TN1 and N2 segments,using the matching method described above. This data was then used tocompute the segment usage weights via Equation 2. Segments wereprioritized for inclusion in the physical library based on theirrelative frequency of occurrence in the CDRH3 sequences of the HPS (asindicated by the segment usage weights), as well as other factors (morefully described below), such as hydrophobicity, alpha-helicalpropensity, and expressibility in yeast.

Example 9.1 Exemplary Library Design (ELD-1)

ELD-1 uses the HPS and the segments from TSP1 1 (9.5×10⁹ members) asinputs and produces an output of 100 TN1, 200 DH, 141 N2 and 100 H3-JHsegments, each from TSP1, ranked in order by their usage weights in theHPS, to produce a library with theoretical complexity of 2.82×10⁸. Thesegments corresponding to ELD-1 are provided in Table 23. Note that herethe combination of all of the segments (i.e., TN1, DH, N2, and H3-JH),and the individual sets of segments (i.e., TN1 only, DH only, N2 only,and H3-JH only) each constitute theoretical segment pools.

Example 9.2 Exemplary Library Design 2 (ELD-2)

The inputs for this design are the HPS and the segments from TSP1 plusthe novel TN1 and N2 segments extracted from the HPS (Example 7). Theoutputs are (1) 200 DH and 100 H3-JH segments, each from TSP1; and (2)100 TN1 and 200 N2 segments including TN1 and N2 segments originally inTSP1 and those extracted from the sequences in the HPS. Applying themethod described in Example 7 to extract novel TN1 and N2 segments(i.e., those not included in TSP1) resulted in the identification of1,710 novel TN1 segments and 1,024 novel N2 segments. The segmentscorresponding to ELD-2 are provided in Table 24. Note that here thecombination of all of the segments (i.e., TN1, DH, N2, and H3-JH), andthe individual sets of segments (i.e., TN1 only, DH only, N2 only, andH3-JH only) each constitute theoretical segment pools. As in ELD-1, allsegments in ELD-2 were selected for inclusion based solely on theirusage weights in the HPS.

Example 9.3 Exemplary Library Design 3 (ELD-3)

The inputs for this design are identical to those for ELD-2. As inELD-2, the outputs are (1) a set of 200 DH and 100 H3-JH segments, eachfrom TSP1; and (2) a set of 100 TN1 and 200 N2 segments, including TN1and N2 segments originally in TSP1 and those extracted from thesequences in the HPS (Example 7). However, the approach used for theselection of the segments for ELD-3 differs in two respects. First,selected physicochemical properties of the segments (hydrophobicity,isoelectric point, and alpha-helix propensity) were used, in addition tothe segment usage weights, to prioritize segments for inclusion in thephysical library. Hydrophobicity was used to de-prioritize hydrophobicDH segments that are empirically over-represented in poorly expressedantibodies isolated from yeast-based libraries. Isoelectric point andpropensity for alpha-helix formation were utilized to identify segmentslocated in regions of physicochemical property space that wererelatively unexplored in CDRH3 libraries known in the art (e.g., U.S.Publication Nos. 2009/0181855 and 2010/0056386, and WO/2009/036379).Second, the segment usage weights were calculated via a bootstrapanalysis of the HPS dataset. These methods are more fully describedbelow. The segments corresponding to ELD-3 are provided in Table 25.Note that here the combination of all of the segments (i.e., TN1, DH,N2, and H3-JH), and the individual sets of segments (i.e., TN1 only, DHonly, N2 only, and H3-JH only) each constitute theoretical segmentpools.

Example 9.3.1 Generation of Segment Usage Weights Via Bootstrap Analysis

Bootstrap analysis (Efron & Tibshirani, An Introduction to theBootstrap, 1994 Chapman Hill, New York) is a widely used statisticalprocedure for estimating the variability of a statistic of a givensample. This estimate is based on the value of the statistic calculatedfor several sub-samples, equal in size to the original sample andderived from it via sampling with replacement. Members of the originalsample are chosen at random to form the sub-samples, and are typicallyincluded multiple times in each sub-sample (hence, “sampling withreplacement”).

Here, the original sample is the HPS dataset with n=3,571 members andthe statistic is the segment usage weight. One-thousand sub-samples,each with 3,571 members, were generated by randomly choosing sequencesfrom the HPS dataset (no more than 10 repeats of a given sequence wereallowed in each sub-sample). The matching method described above wasthen applied to each sub-sample, and the final segment usage weightswere calculated as the average of the values obtained for the individualsub-samples. Average values derived via this bootstrap procedure aremore robust than values calculated from the parent HPS dataset alone.Unless indicated otherwise, these average values of the 1,000sub-samples were used in the selection of segments for ELD-3.

Example 9.3.2 Amino Acid Property Indices

The AAindex database, available online at www.genome.ip/aaindex/,provides more than 500 numerical indices representing variousphysicochemical and biochemical properties of amino acids and pairs ofamino acids. These properties include hydrophobicity, electrostaticbehavior, secondary structure propensities and other characteristics,with several indices often available for a given property. The followingthree indices were chosen by starting with the well-understoodKyte-Doolittle hydropathy index (KYJT820101) and adding the indices mostnumerically de-correlated from it and each other. They thus potentiallydescribe non-overlapping regions of amino acid property space and wereused for analysis and selection of the DH and H3-JH segments for ELD-3:

1. KYTJ820101 (hydropathy index)

2. LEVM780101(normalized frequency of alpha helix)

3. ZIMJ680104 (isoelectric point)

Example 9.3.3 Hydrophobic DH Segments are Over-Represented in PoorlyExpressed Antibodies Isolated from Yeast-Based Libraries

Based on protein expression levels from approximately 1200 antibodiesexpressed in S. cerevisiae, antibodies were classified as either “Good”or “Poor” expressors. The CDRH3 sequence of each antibody in eachcategory was examined to identify sequence features that correlated withthe expression level. One such sequence feature is the hydrophobicity ofthe DH segments calculated using the KYTJ820101 index. FIG. 6 providesthe frequency of “Good” and “Poor” expressors as a function of the DHsegment hydrophobicity (increasing to the right). The distributionexpected from the synthetic library used to isolate these antibodies isalso provided as a reference (“Design”). DH segments with the highesthydrophobicity values (far right of the plot) are over-represented(relative to the expectation based on the design) among “Poor”expressors and under-represented among “Good” expressors. Similarly,hydrophilic DH segments (far left) are over-represented among “Good”expressors and under-represented among “Poor” expressors. From thisdata, it was inferred that the overall expressibility of the antibodiesof the library may be improved by synthesizing CDRH3 sequences withfewer hydrophobic DH segments.

Example 9.3.4 Selection of the 200 DH Segments for Inclusion in ELD-3

A set of 71 DH segments from TSP1 were designated as “core” DH segmentsfor automatic inclusion in ELD-3. These segments had the followingdesirable properties:

-   -   1. Fifty-three of seventy-one were present within the top 7% of        DH segments rank-ordered by segment usage weights from the        bootstrap analysis.    -   2. Eighteen of seventy-one were present within the top 7% of DH        segments rank-ordered by usage weights derived from antibodies        isolated from libraries expressed in S. cerevisiae.

The remaining 1,040 segments were designated as “non-core.” To completethe set of 200 segments in ELD-3, 129 segments were chosen from the“non-core” pool of segments in the following manner:

-   -   1. Sixty-five segments were eliminated because they contain        either (a) an Asn residue at the last or next-to-last position        with the potential to form N-linked glycosylation motifs via        combination with N2 amino acids or (b) the amino acid sequence        NG, implicated in de-amidation.    -   2. Segments with higher than median values for the KYTJ820101        hydropathy index (median=2.9 for 1K DH) were eliminated from        further consideration. In view of the known importance of Tyr        for antigen recognition (Fellouse et al., PNAS, 2004, 101:        12467; and Hofstadter et al., J. Mol. Biol., 1999, 285: 805,        each incorporated by reference in its entirety), segments        containing at least one Tyr residue were retained unless located        in the highest hydrophobicity quartile (KYTJ820101 value higher        than 9.4). This eliminated 443 segments.    -   3. The final set of 129 segments was obtained by using an        objective function that aimed to maximize the Euclidean        distance, between the “core” and the remaining 443 “non-core”        segments, in a multi-dimensional space defined by the following        variables: (1) amino acid mismatches to nearest neighbor;        and (2) values of the three physicochemical property indices.

Example 9.3.5 Selection of the 100 H3-JH Segments for Inclusion in ELD-3

One-hundred H3-JH segments were chosen for inclusion in ELD-3 in thefollowing manner.

-   -   1. Twenty-eight H3-JH segments were selected after being        experimentally validated in other libraries containing only        these H3-JH segments (see U.S. Publication Nos. 2009/0181855 and        2010/0056386, and WO/2009/036379).    -   2. Fifty-seven segments were selected based on their presence        within the top 25% of H3-JH segments rank-ordered by usage        weights from the bootstrap analysis described above. These 57        H3-JH segments, plus the 28 H3-JH segments of (1) (i.e., 85        segments total) were designated as the “core” H3-JH segments,        which, like the core DH segments, were automatically included in        ELD-3.    -   4. Fifteen additional segments were chosen by using an objective        function that aimed to maximize the Euclidian distance, between        the “core” and the remaining 200 “non-core” segments, in a        multi-dimensional space defined by the following variables: (1)        amino acid mismatches to nearest neighbor; and (2) values of the        three physicochemical property indices.

Example 9.3.6 Selection of 100 TN1 and 200 N2 Segments for Inclusion inELD-3

TN1 and N2 segments were extracted from the sequences in each sub-sampleof the bootstrap procedure, and the 100 TN1 and 200 N2 segments with thehighest average segment usage weights were chosen for inclusion into thelibrary, after elimination of sequences with undesirable motifs, namelyCys and Asn residues.

Example 9.3.7 Selection of Nucleotide Sequences to Encode the SegmentsChosen for Inclusion in ELD-3

Each of the polypeptide segments chosen for inclusion in the librarymust be back translated (polypeptide to DNA) into a correspondingoligonucleotide sequence. While a large number of oligonucleotides couldpossibly encode each polypeptide segment, due to the degeneracy of thegenetic code, certain constraints were imposed to selectoligonucleotides that were more desirable. First, since ELD-3 wasexpressed in yeast (S. cerevisiae), codons that are rarely used in yeastwere avoided. For example, of the six possible codons for Arg, three:CGA, CGC and CGG are used to encode yeast proteins at rates of under 10%(see, for example, Nakamura et al., Nucleic Acids Res., 2000, 28:292),and therefore those three codons were avoided to the extent possible.Second, since many antibodies are produced in Chinese Hamster Ovary(CHO) cells (after discovery e.g., in yeast), the CCG codon (encodingPro) was also avoided, since it is rarely used by hamsters (Nakamura etal.)

A number of restriction enzymes are employed during the actualconstruction of the CDRH3 oligonucleotide library (see Example 10 ofU.S. Pub. No. 2009/0181855). It is thus desirable to avoid theoccurrence of recognition motifs for these restriction enzymes withinthe CDRH3 polynucleotide sequences. Codons are selected at theindividual segment level to avoid introducing recognition motifs forrestriction enzymes that may be used downstream. Since such motifs mayalso be generated by combinatorial assembly of the segments, the segmentcombinations are also checked and, whenever possible, codons are changedto eliminate the occurrence of such motifs. Specifically, threerestriction enzymes were used during the construction of the currentlyexemplified CDRH3 library: BsrDI, BbsI, and AvrII. The first two aretype II enzymes with non-palindromic recognition sites. The reversestrand of the oligonucleotides encoding the segments was checkedexplicitly for recognition sites for these two enzymes. In particular,the reverse strands were checked for the motifs GCAATG and CATTGC (forBsrDI) and GAAGAC and GTCTTC (for BbsI). The recognition motif for AvrIIis palindromic so the oligonucleotides were only checked for thesequence CCTAGG. However, AvrII is used only to treat TN1 segments, andthus it is not necessary to evaluate its presence in the other segmentsor their combinations.

An additional constraint that was imposed to improve engineering of thepolypeptide to polynucleotide conversion was avoidance of consecutiveruns of 6 or more of the same type of base, as this is believed toincrease errors during solid phase oligonucleotide synthesis. Therefore,DNA sequences for the segments of ELD-3 were chosen to avoid suchmotifs. The DNA sequences for the ELD-3 segments are included, with therespective polypeptide sequences, in Table 25. One of ordinary skill inthe art will readily recognize that these methods can also be applied toany other library, any restriction sites, any number of nucleotiderepeats, and/or to avoid the occurrence of any codons consideredundesirable in any organism.

Example 10 Matching of ELD-3 to Human CDRH3 Datasets and ClinicallyRelevant Antibodies

Among the objectives of the invention is to mimic the V-D-Jrecombination processes underlying the creation of the human CDRH3repertoire in vivo, thereby increasing the diversity of the CDRH3library in comparison to other libraries known in the art, whilemaintaining the human character of CDRH3. One measure of success is theextent to which collections of human reference CDRH3 sequences arerepresented identically, or via close matches (e.g., less than about 5,4, 3, or 2 amino acid differences) in any library of the invention. Weevaluated this metric using two human CDRH3 sequence reference datasets,both non-overlapping with each other and the HPS: (1) a collection of666 human CDRH3 sequences (Lee et al., Immunogenetics, 2006, 57: 917;“Lee-666”); and (2) a collection of 3,000 human CDRH3 sequences randomlychosen from the over 200,000 sequences disclosed in Boyd et al., ScienceTranslational Medicine, 2009, 1: 1-8 (“Boyd-3000”). The results of therandom sample of the 3,000 human CDRH3 sequences from Boyd et al. wasrepresentative of the results of the same analysis as applied to allmembers of the Boyd et al. set (>200,000 CDRH3 sequences).

FIG. 7 provides the percentage of CDRH3 sequences in two syntheticlibraries, “LUA-141” and ELD-3, that match a sequence from the Lee-666or Boyd-3000 sets with zero, one, two, three, or more than three aminoacid mismatches. Here, “LUA-141” represents a library containing 212TN1, 278 DH, 141 N2, and 28 H3-JH (see U.S. Publication No. 2009/0181855for details). In particular, it is notable that ELD-3 exhibits a higherpercentage of sequences (12.9% and 12.1% for the Lee-666 and Boyd-3000sets, respectively) that identically match a reference CDRH3 sequencethan LUA-141 (8.4% and 6.3% for the Lee-666 and Boyd-3000 sets,respectively). It is also notable that ELD-3 exhibits a highercumulative percentage of human CDRH3 sequences found with no more thantwo amino acid mismatches (54.1% and 52.5% for the Lee-666 and Boyd-3000sets, respectively) relative to LUA-141 (41.2% and 43.7% for the Lee-666and Boyd-3000 sets, respectively).

Another metric by which antibody libraries can be evaluated is theirability to match “clinically relevant” reference CDRH3 sequences. FIG. 8demonstrates that ELD-3 returns better matches to clinically relevantCDRH3 sequences than the LUA-141 library. Specifically, ELD-3 matches 34of 55 (62%) clinically validated antibodies within one amino acid, whilethe LUA-141 library only matches 20 of 55 (37%).

Example 11 Comparison of ELD-3 to LUA-141

ELD-3 has 73 TN1, 92 DH, 119 N2, and 28 H3-JH in common with LUA-141.Thus, 94.5% of the sequences in ELD-3 (4.0×10⁸ members) are differentfrom the LUA-141 library (2.3×10⁸ members). FIG. 9 demonstrates that thecombinatorial efficiency of the segments in ELD-3 is greater than thatof the segments in LUA-141. Specifically, the ELD-3 segments are morelikely to yield a unique CDRH3 than the LUA-141 library segments. Thisis advantageous, because it allows one to synthesize libraries withincreased CDRH3 diversity using fewer segments.

FIG. 10 provides the amino acid compositions of the Kabat-CDRH3s ofLUA-141, ELD-3, and Human CDRH3 sequences from the HPS.

FIG. 11 provides the Kabat-CDRH3 length distribution of LUA-141, ELD-3,and Human CDRH3 sequences from the HPS.

CDRH3 Libraries Synthesized with Degenerate Oligonucleotides

Example 12 Further Increasing CDRH3 Diversity by Utilizing DegenerateOligonucleotides

The methods described in this example extend the methods taught above,to produce CDRH3 libraries with more members than those of the librariesdescribed above. In particular, one or two degenerate codons wereintroduced into the DH and or N2 polynucleotide segments, and(generally) no degenerate codon or one degenerate codon were introducedinto the H3-JH segments. Segments with different numbers of degeneratecodons are also contemplated; for example DH segments with 0, 1, 2, 3,4, 5, 6, 7, 8, or more degenerate codons, and H3-JH segments with 0, 1,2, 3, 4, 5, or more degenerate codons. This results in CDRH3 librariescontaining greater than about 10¹¹ (about 2×10¹¹) distinct CDRH3 aminoacid sequences that closely reflect properties, such as length andcomposition among others, of a reference set of human CDRH3 sequences.As described below, the degenerate positions in the DH segments areusually, but not always, the very N- and/or C-terminal positions, or 5′and 3′ end codons (i.e., not necessarily only the first or last base),respectively, when considering the corresponding oligonucleotidesequences. Degenerate codons were similarly used to synthesize N2segments. Two hundred of the TN1 segments were as described in ELD-3,although libraries with degenerate TN1 segments, or with alternativechoices of TN1 segment sequences, fall within the scope of theinvention. An additional one hundred TN1 segments complete the set of300 TN1 segments for this library. The amino acid and nucleotidesequences are listed in Table 26. It is also possible to use mixtures oftrinucleotides instead of, or in addition to, degenerateoligonucleotides in order to allow amino acid type variation at one ormore selected positions within “base” or “seed” segment sequences(defined below).

Example 13 Selection of DH Segments for Synthesis by DegenerateOligonucleotides

The segment usage weights were calculated for the 68K DH TheoreticalSegment Pool by comparison to the sequences contained in Boyd et al. TheDH segments with a length of three or more amino acids were rankedaccording to their segment usage weights (as described above), and thetop 201 were designated as “seed” sequences. These seed sequences werethen varied by selecting certain positions to incorporate degeneratecodons. The positions selected for variance, the amino acids types towhich they were varied, were determined by comparing the seed sequencesto a reference set of 9,171 DH segments that were a subset of the 68K DHTheoretical Segment Pool. These 9,171 DH segments were selected becausetheir segment usage weight in Boyd et al. was significant, meaning thatthe cumulative segment usage weight (Example 8) is at least 1.0.

Each of the 201 seed sequences was compared to each of the sequences inthe reference set of 9,171 DH segments, and those of identical lengthand differing at a single position were further considered to informpossible variants of the seed. In this manner, the most variableposition for each seed was identified and a set of candidate amino acidtypes was also identified for each position. Finally, a set ofdegenerate codons was considered, to identify the codon that mostfaithfully represented the set of candidate amino acid types for eachparticular position. Degenerate codons encoding stop codons, Cysresidues, N-linked glycosylation motifs (i.e., NXS or NXT, where X isany amino acid type), or deamidation motifs (NG) were eliminated fromconsideration. This process generated 149 unique degenerateoligonucleotide sequences, which collectively encode 3,566 uniquepolypeptide sequences. Alternative designs generated according to thesame principles were also considered, and those having a largerdiversity (in terms of the number of unique polypeptide sequences) andsmaller RMAX values (see below) were given preference for inclusion inthe libraries of the invention. However, it is also conceivable thatdifferent criteria could be used to select DH segments from the 68K DHTheoretical Segment Pool, and that libraries including DH segmentsselected by these different criteria would also fall within the scope ofthe invention.

Because not all degenerate oligonucleotides encode an identical numberof polypeptides, the latter do not occur with uniformly identicalweights over the entirety of a given theoretical segment pool (i.e.,TN1, DH, N2 and H3-JH) contained within a CDRH3 library of theinvention. For example, an individual amino acid sequence X encoded byan oligonucleotide of total degeneracy 4 will have a “weight” of ¼,while another individual amino acid sequence, Y, encoded by anoligonucleotide of degeneracy 6 will have a weight of ⅙. Moreover,certain amino acid sequences could be encoded by more than onedegenerate oligonucleotide, so their weights will be the sum of theindividual contributions by each oligonucleotide. Within a giventheoretical segment pool, the ratio of the weight of the most heavilyweighted polypeptide to that of the least heavily weighted one, RMAX, isan important design criterion that one would ideally like to minimize.The RMAX value may be defined by length, or overall for all of thesegments of any given type (i.e., all the DH segments, or all the H3-JHsegments, and so on for the TN1, and/or the N2 segments). Table 27 liststhe degenerate oligonucleotide sequences, while Table 28 lists theunique polypeptide sequences resulting from these oligonucleotides.These two tables include the DH dimer segments the design of which isdetailed below.

Example 13.1 Selection of DH Dimer Segments

A different method was employed to design a set of degenerateoligonucleotides encoding DH dimer sequences. The method aimed toinclude all of the 45 dimer sequences in ELD-3 plus as many of the other400 theoretically possible dimer sequences (i.e., 20 residues possiblein each of 2 positions=20*20), minus segments containing Asn (N)residues and excessively hydrophobic dimers (i.e., any dimer combinationcomprising only F, I, L, M, and/or V residues). This design processultimately yielded 35 degenerate oligonucleotide sequences encoding 213unique peptide dimer sequences. As with the selection processes used forall of the other segments of the invention, one or ordinary skill in theart will readily recognize that other criteria could be used to selectthe DH dimer segments, and that libraries including these segments alsofall within the scope of the invention.

Combining the DH dimer segments with the longer DH segments of Example13, yielded the final set of DH segments of the currently exemplifiedlibrary, encoded by a total of 184 oligonucleotides (35 encoding dimersand 149 encoding segments having three or more amino acids) versus the200 oligonucleotides of ELD-3. The 184 oligonucleotides encode a totalof 3,779 unique polypeptide sequences: 213 dimers and 3,566 longersegments of three amino acids or greater.

Example 14 Generation of Expanded N2 Diversity

As described above, ELD-3 contains 200 N2 segments. In the currentlyexemplified library, the empty N2 segment (i.e., no N2, so that the DHsegments are joined directly to the H3-JH segments) and monomer N2segments were the same as in ELD-3. However, degenerate oligonucleotideswere used to generate sets of two-, three-, and four-mers that not onlyrecapitulated all of the corresponding sequences in ELD-3 but alsoresulted in additional diversity. As with the DH segments, thesedegenerate oligonucleotides were designed to eliminate Asn (inunsuitable positions) and Cys residues, and stop codons. Morespecifically, Asn residues were allowed at the first position of trimersand at the first or second position of tetramers whenever the subsequentamino acid was not Gly and the next amino acid was not Ser or Thr, thusavoiding deamidation or N-linked glycosylation motifs within thecandidate N2 segments. The N2 theoretical segment pool for the currentlyexemplified library contains one zero-mer (i.e., no N2 segment), 18monomer, 279 dimer, 339 trimers, and 90 tetramer N2 amino acidsequences, or 727 segments in total. These amino acid sequences areencoded by 1, 18, 81, 36, and 10 oligonucleotides, respectively, for atotal of 146 oligonucleotides. All but the first 19 oligonucleotides,those encoding the zero- and one-mers, are degenerate. Table 29 liststhe 146 oligonucleotide sequences, while Table 30 lists the resulting727 unique polypeptide sequences.

Example 15 Generation of Expanded H3-JH Diversity

Application of nucleotide-level progressive deletions on the 5′ end ofthe human IGHJ polynucleotide segments down to the point where only theDNA sequence corresponding to FRM4 remained (i.e., no H3-JH remained),followed by systematic 1- or 2-bp completions on the same 5′ end,resulted in 643 unique H3-JH peptide segments after translation (“643H3-JH Set”). As done with the DH segments, it is possible to rank ordereach of the 643 segments by their usage weights obtained aftercomparison to the approximately 237,000 human sequences from Boyd etal., and the top 200 individual sequences, from those devoid of theundesired motifs described above, were chosen to provide the set ofH3-JH segments for the currently exemplified library.

In an alternatively exemplified embodiment, 46 of the 200 H3-JH segmentswere designed with a two-fold degenerate codon in the first position(i.e., N-terminal or 5′ end, respectively, at the peptide andoligonucleotide level), so that, overall, 200 oligonucleotides wouldencode 246 unique peptide sequences.

In yet other alternatively exemplified embodiments, further use ofdegenerate codons may be conceived to produce libraries encoded by 90,100, 200 or more oligonucleotides representing up to 500 distinctpolypeptide sequences. Preferably, but not necessarily, these up to 500unique sequences could be a subset of the sequences in the 643 H3-JHreference set described above, or a subset of variants of thesesequences. As exemplified above, H3-JH segments containing undesirablepolypeptide motifs may be eliminated from the design. Theoligonucleotide sequences for the JH segments are listed on Table 31,while the resulting unique polypeptide sequences are provided in Table32. In Table 31, nucleotide sequences corresponding to the FRM4 regionare also provided, but the “peptide length” value refers to the H3-JHportion only. For simplicity, only the H3-JH peptide sequences areincluded in Table 32.

Example 16 Extended Diversity Library Design (EDLD)

The TN1, DH, H3-JH, and N2 segments selected above, and provided inTables 26 to 32, were combined to generate an Extended Diversity LibraryDesign (EDLD) with theoretical diversity of about 2×10¹¹ (300 TN1×3,779DH×727 N2×246 H3-JH). The oligonucleotides encoding the selectedsegments were chosen according to the principles of Example 9.3.7.

FIGS. 12-15 illustrate certain characteristics of this designindicating, for example, that about 50% of the approximately 237,000CDRH3 sequences in Boyd et al. may be recapitulated by library sequenceswith either one or no mismatches (i.e., by summing the “0” and “1” binsof FIG. 12). The theoretical length distributions (FIG. 13) and aminoacid compositions (FIG. 14) of these libraries also match closely therespective characteristics observed in the same set of human CDRH3sequences. FIG. 15 shows the combinatorial efficiency of the ExtendedDiversity Library Design. Approximately 65% of the sequences appear onlyonce in the design (i.e., are generated via one non-degeneratecombination of segments). FIG. 8, previously presented, shows that theExtended Diversity Library Design outperforms both LUA-141 and ELD-3 interms of matching to clinically relevant human antibody sequences.

TABLE 1 Germline-like sequences for eight of theVK chassis provided by the invention. Germline-Like SEQ ID GermlineJunction CDRL3 Sequence NO VK1-05 1 QQYNSYST 1 VK1-05 2 QQYNSYFT 2VK1-05 3 QQYNSYLT 3 VK1-05 4 QQYNSYIT 4 VK1-05 5 QQYNSYRT 5 VK1-05 6QQYNSYWT 6 VK1-05 7 QQYNSYYT 7 VK1-05 8 QQYNSYSPT 8 VK1-05 9 QQYNSYSFT 9VK1-05 10 QQYNSYSLT 10 VK1-05 11 QQYNSYSIT 11 VK1-05 12 QQYNSYSRT 12VK1-05 13 QQYNSYSWT 13 VK1-05 14 QQYNSYSYT 14 VK1-05 15 QQYNSYSPFT 15VK1-05 16 QQYNSYSPLT 16 VK1-05 17 QQYNSYSPIT 17 VK1-05 18 QQYNSYSPRT 18VK1-05 19 QQYNSYSPWT 19 VK1-05 20 QQYNSYSPYT 20 VK1-12 1 QQANSFPT 21VK1-12 2 QQANSFFT 22 VK1-12 3 QQANSFLT 23 VK1-12 4 QQANSFIT 24 VK1-12 5QQANSFRT 25 VK1-12 6 QQANSFWT 26 VK1-12 7 QQANSFYT 27 VK1-12 8 QQANSFPPT28 VK1-12 9 QQANSFPFT 29 VK1-12 10 QQANSFPLT 30 VK1-12 11 QQANSFPIT 31VK1-12 12 QQANSFPRT 32 VK1-12 13 QQANSFPWT 33 VK1-12 14 QQANSFPYT 34VK1-12 15 QQANSFPPFT 35 VK1-12 16 QQANSFPPLT 36 VK1-12 17 QQANSFPPIT 37VK1-12 18 QQANSFPPRT 38 VK1-12 19 QQANSFPPWT 39 VK1-12 20 QQANSFPPYT 40VK1-33 1 QQYDNLPT 41 VK1-33 2 QQYDNLFT 42 VK1-33 3 QQYDNLLT 43 VK1-33 4QQYDNLIT 44 VK1-33 5 QQYDNLRT 45 VK1-33 6 QQYDNLWT 46 VK1-33 7 QQYDNLYT47 VK1-33 8 QQYDNLPPT 48 VK1-33 9 QQYDNLPFT 49 VK1-33 10 QQYDNLPLT 50VK1-33 11 QQYDNLPIT 51 VK1-33 12 QQYDNLPRT 52 VK1-33 13 QQYDNLPWT 53VK1-33 14 QQYDNLPYT 54 VK1-33 15 QQYDNLPPFT 55 VK1-33 16 QQYDNLPPLT 56VK1-33 17 QQYDNLPPIT 57 VK1-33 18 QQYDNLPPRT 58 VK1-33 19 QQYDNLPPWT 59VK1-33 20 QQYDNLPPYT 60 VK1-39 1 QQSYSTPT 61 VK1-39 2 QQSYSTFT 62 VK1-393 QQSYSTLT 63 VK1-39 4 QQSYSTIT 64 VK1-39 5 QQSYSTRT 65 VK1-39 6QQSYSTWT 66 VK1-39 7 QQSYSTYT 67 VK1-39 8 QQSYSTPPT 68 VK1-39 9QQSYSTPFT 69 VK1-39 10 QQSYSTPLT 70 VK1-39 11 QQSYSTPIT 71 VK1-39 12QQSYSTPRT 72 VK1-39 13 QQSYSTPWT 73 VK1-39 14 QQSYSTPYT 74 VK1-39 15QQSYSTPPFT 75 VK1-39 16 QQSYSTPPLT 76 VK1-39 17 QQSYSTPPIT 77 VK1-39 18QQSYSTPPRT 78 VK1-39 19 QQSYSTPPWT 79 VK1-39 20 QQSYSTPPYT 80 VK4-01 1QQYYSTPT 81 VK4-01 2 QQYYSTFT 82 VK4-01 3 QQYYSTLT 83 VK4-01 4 QQYYSTIT84 VK4-01 5 QQYYSTRT 85 VK4-01 6 QQYYSTWT 86 VK4-01 7 QQYYSTYT 87 VK4-018 QQYYSTPPT 88 VK4-01 9 QQYYSTPFT 89 VK4-01 10 QQYYSTPLT 90 VK4-01 11QQYYSTPIT 91 VK4-01 12 QQYYSTPRT 92 VK4-01 13 QQYYSTPWT 93 VK4-01 14QQYYSTPYT 94 VK4-01 15 QQYYSTPPFT 95 VK4-01 16 QQYYSTPPLT 96 VK4-01 17QQYYSTPPIT 97 VK4-01 18 QQYYSTPPRT 98 VK4-01 19 QQYYSTPPWT 99 VK4-01 20QQYYSTPPYT 100 VK2-28 1 MQALQTPT 101 VK2-28 2 MQALQTFT 102 VK2-28 3MQALQTLT 103 VK2-28 4 MQALQTIT 104 VK2-28 5 MQALQTRT 105 VK2-28 6MQALQTWT 106 VK2-28 7 MQALQTYT 107 VK2-28 8 MQALQTPPT 108 VK2-28 9MQALQTPFT 109 VK2-28 10 MQALQTPLT 110 VK2-28 11 MQALQTPIT 111 VK2-28 12MQALQTPRT 112 VK2-28 13 MQALQTPWT 113 VK2-28 14 MQALQTPYT 114 VK2-28 15MQALQTPPFT 115 VK2-28 16 MQALQTPPLT 116 VK2-28 17 MQALQTPPIT 117 VK2-2818 MQALQTPPRT 118 VK2-28 19 MQALQTPPWT 119 VK2-28 20 MQALQTPPYT 120VK3-11 1 QQRSNWPT 121 VK3-11 2 QQRSNWFT 122 VK3-11 3 QQRSNWLT 123 VK3-114 QQRSNWIT 124 VK3-11 5 QQRSNWRT 125 VK3-11 6 QQRSNWWT 126 VK3-11 7QQRSNWYT 127 VK3-11 8 QQRSNWPPT 128 VK3-11 9 QQRSNWPFT 129 VK3-11 10QQRSNWPLT 130 VK3-11 11 QQRSNWPIT 131 VK3-11 12 QQRSNWPRT 132 VK3-11 13QQRSNWPWT 133 VK3-11 14 QQRSNWPYT 134 VK3-11 15 QQRSNWPPFT 135 VK3-11 16QQRSNWPPLT 136 VK3-11 17 QQRSNWPPIT 137 VK3-11 18 QQRSNWPPRT 138 VK3-1119 QQRSNWPPWT 139 VK3-11 20 QQRSNWPPYT 140 VK3-15 1 QQYNNWPT 141 VK3-152 QQYNNWFT 142 VK3-15 3 QQYNNWLT 143 VK3-15 4 QQYNNWIT 144 VK3-15 5QQYNNWRT 145 VK3-15 6 QQYNNWWT 146 VK3-15 7 QQYNNWYT 147 VK3-15 8QQYNNWPPT 148 VK3-15 9 QQYNNWPFT 149 VK3-15 10 QQYNNWPLT 150 VK3-15 11QQYNNWPIT 151 VK3-15 12 QQYNNWPRT 152 VK3-15 13 QQYNNWPWT 153 VK3-15 14QQYNNWPYT 154 VK3-15 15 QQYNNWPPFT 155 VK3-15 16 QQYNNWPPLT 156 VK3-1517 QQYNNWPPIT 157 VK3-15 18 QQYNNWPPRT 158 VK3-15 19 QQYNNWPPWT 159VK3-15 20 QQYNNWPPYT 160 VK3-20 1 QQYGSSPT 161 VK3-20 2 QQYGSSFT 162VK3-20 3 QQYGSSLT 163 VK3-20 4 QQYGSSIT 164 VK3-20 5 QQYGSSRT 165 VK3-206 QQYGSSWT 166 VK3-20 7 QQYGSSYT 167 VK3-20 8 QQYGSSPPT 168 VK3-20 9QQYGSSPFT 169 VK3-20 10 QQYGSSPLT 170 VK3-20 11 QQYGSSPIT 171 VK3-20 12QQYGSSPRT 172 VK3-20 13 QQYGSSPWT 173 VK3-20 14 QQYGSSPYT 174 VK3-20 15QQYGSSPPFT 175 VK3-20 16 QQYGSSPPLT 176 VK3-20 17 QQYGSSPPIT 177 VK3-2018 QQYGSSPPRT 178 VK3-20 19 QQYGSSPPWT 179 VK3-20 20 QQYGSSPPYT 180

TABLE 2 Summary of framework variants for exemplified light chaingermlines. Primary Alternative Framework Framework Number of PositionsPositions Light Chain Sequences Selected for Selected for GermlineAnalyzed Variance Variance VK1-5  307 4, 49 46 VK1-12 113 4, 49 46, 66 VK1-33 188 4, 66 49 VK1-39 656 4, 49 46 VK2-28 275 46, 49  2, 4  VK3-11375 4, 36 2, 49 VK3-15 202 4, 49 2, 48 VK3-20 867 4, 49 2, 48 VK4-1  3684, 49 46, 66 

TABLE 3Polypeptide sequences of exemplified light chain chassis with variability in CDRL1,CDRL2, and frameworks. The Kabat numbers for segment boundaries areindicated. Here, L1 and L2 (in the “Category”column) indicate variability in CDRL1 and CDRL2, respectively, while “F”indicates a framework variant.Sequences designated with both L1 or L2 and F contain variability in both a CDRand framework region. Name Chassis Category FRM1: 1-23 CDR1: 24-34FRM2: 35-49 CDR2: 50-56 FRM3: 57-88 SEQ ID NOs: VK1- VK1-39 GermlineDIQMTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 181 39VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39 L1 DIQMTQSPSSLSASRASQSINSYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 182 39.1 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-39 L1 DIQMTQSPSSLSAS RASQSIDSYLNWYQQKPGKAPK AASSLQS GVPSRFSGSGS 183 39.2 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-39 L1 DIQMTQSPSSLSAS RASQSISRYLN WYQQKPGKAPK AASSLQSGVPSRFSGSGS 184 39.3 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39L2 DIQMTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK GASSLQS GVPSRFSGSGS 185 39.6VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39 L2 DIQMTQSPSSLSASRASQSISSYLN WYQQKPGKAPK SASSLQS GVPSRFSGSGS 186 39.7 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-39 L2 DIQMTQSPSSLSAS RASQSISSYLNWYQQKPGKAPK AASNLQS GVPSRFSGSGS 187 39.8 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-39 F DIQLTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK AASSLQSGVPSRFSGSGS 188 39.10 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39FL1 DIQLTQSPSSLSAS RASQSINSYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 189 39.11VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39 FL1 DIQLTQSPSSLSASRASQSIDSYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 190 39.12 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-39 FL1 DIQLTQSPSSLSAS RASQSISSFLNWYQQKPGKAPK AASSLQS GVPSRFSGSGS 191 39.15 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-39 FL2 DIQLTQSPSSLSAS RASQSISSYLN WYQQKPGKAPKSASSLQS GVPSRFSGSGS 192 39.17 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1-VK1-39 FL2 DIQLTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK AASNLQS GVPSRFSGSGS193 39.18 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-39 FDIQMTQSPSSLSAS RASQSISSYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 194 39.20VGDRVTITC LLIS GTDFTLTISSL QPEDFATYYC VK1- VK1-39 FL1 DIQMTQSPSSLSASRASQSISRYLN WYQQKPGKAPK AASSLQS GVPSRFSGSGS 195 39.23 VGDRVTITC LLISGTDFTLTISSL QPEDFATYYC VK1- VK1-39 FL1 DIQMTQSPSSLSAS RASQSISIYLNWYQQKPGKAPK AASSLQS GVPSRFSGSGS 196 39.24 VGDRVTITC LLIS GTDFTLTISSLQPEDFATYYC VK1- VK1-39 FL1 DIQMTQSPSSLSAS RASQSISSFLN WYQQKPGKAPKAASSLQS GVPSRFSGSGS 197 39.25 VGDRVTITC LLIS GTDFTLTISSL QPEDFATYYC VK1-VK1-05 Germline DIQMTQSPSTLSAS RASQSISSWLA WYQQKPGKAPK DASSLESGVPSRFSGSGS 198 05 VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1- VK1-05 L1DIQMTQSPSTLSAS RASQGISSWLA WYQQKPGKAPK DASSLES GVPSRFSGSGS 199 05.1VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1- VK1-05 L2 DIQMTQSPSTLSASRASQSISSWLA WYQQKPGKAPK EASSLES GVPSRFSGSGS 200 05.5 VGDRVTITC LLIYGTEFTLTISSL QPDDFATYYC VK1- VK1-05 L2 DIQMTQSPSTLSAS RASQSISSWLAWYQQKPGKAPK KASSLES GVPSRFSGSGS 201 05.6 VGDRVTITC LLIY GTEFTLTISSLQPDDFATYYC VK1- VK1-05 L12 DIQMTQSPSTLSAS RASQAISSWLA WYQQKPGKAPKKASSLES GVPSRFSGSGS 202 05.7 VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1-VK1-05 L12 DIQMTQSPSTLSAS RASQSINSWLA WYQQKPGKAPK KASSLES GVPSRFSGSGS203 05.8 VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1- VK1-05 L12DIQMTQSPSTLSAS RASQSIGSWLA WYQQKPGKAPK KASSLES GVPSRFSGSGS 204 05.9VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1- VK1-05 F DIQLTQSPSTLSASRASQSISSWLA WYQQKPGKAPK DASSLES GVPSRFSGSGS 205 05.10 VGDRVTITC LLIYGTEFTLTISSL QPDDFATYYC VK1- VK1-05 FL1 DIQLTQSPSTLSAS RASQGISSWLAWYQQKPGKAPK DASSLES GVPSRFSGSGS 206 05.11 VGDRVTITC LLIY GTEFTLTISSLQPDDFATYYC VK1- VK1-05 FL1 DIQLTQSPSTLSAS RASQAISSWLA WYQQKPGKAPKDASSLES GVPSRFSGSGS 207 05.12 VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1-VK1-05 FL1 DIQLTQSPSTLSAS RASQSIGSWLA WYQQKPGKAPK DASSLES GVPSRFSGSGS208 05.14 VGDRVTITC LLIY GTEFTLTISSL QPDDFATYYC VK1- VK1-05 FDIQMTQSPSTLSAS RASQSISSWLA WYQQKPGKAPK DASSLES GVPSRFSGSGS 209 05.20VGDRVTITC LLIS GTEFTLTISSL QPDDFATYYC VK1- VK1-05 FL1 DIQMTQSPSTLSASRASQSINSWLA WYQQKPGKAPK DASSLES GVPSRFSGSGS 210 05.21 VGDRVTITC LLISGTEFTLTISSL QPDDFATYYC VK1- VK1-05 FL2 DIQLTQSPSTLSAS RASQSIGSWLAWYQQKPGKAPK KASSLES GVPSRFSGSGS 211 05.25 VGDRVTITC LLIY GTEFTLTISSLQPDDFATYYC VK1- VK1-05 FL2 DIQMTQSPSTLSAS RASQSISSWLA WYQQKPGKAPKKASSLES GVPSRFSGSGS 212 05.26 VGDRVTITC LLIS GTEFTLTISSL QPDDFATYYC VK1-VK1-12 Germline DIQMTQSPSSVSAS RASQGISSWLA WYQQKPGKAPK AASSLQSGVPSRFSGSGS 213 12 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12 L1DIQMTQSPSSVSAS RASQGIGSWLA WYQQKPGKAPK AASSLQS GVPSRFSGSGS 214 12.2VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12 L1 DIQMTQSPSSVSASRASQGIDSWLA WYQQKPGKAPK AASSLQS GVPSRFSGSGS 215 12.3 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-12 L1 DIQMTQSPSSVSAS RASQGISRWLAWYQQKPGKAPK AASSLQS GVPSRFSGSGS 216 12.4 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-12 L2 DIQMTQSPSSVSAS RASQGISSWLA WYQQKPGKAPK GASSLQSGVPSRFSGSGS 217 12.5 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12L2 DIQMTQSPSSVSAS RASQGISSWLA WYQQKPGKAPK SASSLQS GVPSRFSGSGS 218 12.6VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12 L2 DIQMTQSPSSVSASRASQGISSWLA WYQQKPGKAPK AASNLQS GVPSRFSGSGS 219 12.7 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-12 F DIQLTQSPSSVSAS RASQGISSWLAWYQQKPGKAPK AASSLQS GVPSRFSGSGS 220 12.10 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-12 FL1 DIQLTQSPSSVSAS RASQDISSWLA WYQQKPGKAPKAASSLQS GVPSRFSGSGS 221 12.11 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1-VK1-12 FL1 DIQLTQSPSSVSAS RASQGISRWLA WYQQKPGKAPK AASSLQS GVPSRFSGSGS222 12.14 VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12 FL2DIQLTQSPSSVSAS RASQGISSWLA WYQQKPGKAPK GASSLQS GVPSRFSGSGS 223 12.15VGDRVTITC LLIY GTDFTLTISSL QPEDFATYYC VK1- VK1-12 FL2 DIQLTQSPSSVSASRASQGISSWLA WYQQKPGKAPK SASSLQS GVPSRFSGSGS 224 12.16 VGDRVTITC LLIYGTDFTLTISSL QPEDFATYYC VK1- VK1-12 FL2 DIQLTQSPSSVSAS RASQGISSWLAWYQQKPGKAPK AASNLQS GVPSRFSGSGS 225 12.17 VGDRVTITC LLIY GTDFTLTISSLQPEDFATYYC VK1- VK1-12 F DIQMTQSPSSVSAS RASQGISSWLA WYQQKPGKAPK AASSLQSGVPSRFSGSGS 226 12.20 VGDRVTITC LLIS GTDFTLTISSL QPEDFATYYC VK1- VK1-12FL1 DIQMTQSPSSVSAS RASQDISSWLA WYQQKPGKAPK AASSLQS GVPSRFSGSGS 227 12.21VGDRVTITC LLIS GTDFTLTISSL QPEDFATYYC VK1- VK1-12 FL1 DIQMTQSPSSVSASRASQGIDSWLA WYQQKPGKAPK AASSLQS GVPSRFSGSGS 228 12.23 VGDRVTITC LLISGTDFTLTISSL QPEDFATYYC VK1- VK1-12 FL1 DIQMTQSPSSVSAS RASQGISRWLAWYQQKPGKAPK AASSLQS GVPSRFSGSGS 229 12.24 VGDRVTITC LLIS GTDFTLTISSLQPEDFATYYC VK1- VK1-33 Germline DIQMTQSPSSLSAS QASQDISNYLN WYQQKPGKAPKDASNLET GVPSRFSGSGS 230 33 VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1-VK1-33 L1 DIQMTQSPSSLSAS QASQDITNYLN WYQQKPGKAPK DASNLET GVPSRFSGSGS 23133.1 VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1- VK1-33 L1 DIQMTQSPSSLSASQASQDIANYLN WYQQKPGKAPK DASNLET GVPSRFSGSGS 232 33.2 VGDRVTITC LLIYGTDFTFTISSL QPEDIATYYC VK1- VK1-33 L2 DIQMTQSPSSLSAS QASQDISNYLNWYQQKPGKAPK DASNLAT GVPSRFSGSGS 233 33.8 VGDRVTITC LLIY GTDFTFTISSLQPEDIATYYC VK1- VK1-33 F DIQLTQSPSSLSAS QASQDISNYLN WYQQKPGKAPK DASNLETGVPSRFSGSGS 234 33.10 VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1- VK1-33FL1 DIQLTQSPSSLSAS QASQDISNSLN WYQQKPGKAPK DASNLET GVPSRFSGSGS 235 33.13VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1- VK1-33 FL1 DIQLTQSPSSLSASQASQDISNFLN WYQQKPGKAPK DASNLET GVPSRFSGSGS 236 33.14 VGDRVTITC LLIYGTDFTFTISSL QPEDIATYYC VK1- VK1-33 FL2 DIQLTQSPSSLSAS QASQDISNYLNWYQQKPGKAPK DASNLQT GVPSRFSGSGS 237 33.17 VGDRVTITC LLIY GTDFTFTISSLQPEDIATYYC VK1- VK1-33 F DIQMTQSPSSLSAS QASQDISNYLN WYQQKPGKAPK DASNLETGVPSRFSGSRS 238 33.20 VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1- VK1-33FL1 DIQMTQSPSSLSAS QASQDITNYLN WYQQKPGKAPK DASNLET GVPSRFSGSRS 239 33.21VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK1- VK1-33 FL1 DIQMTQSPSSLSASQASQDIANYLN WYQQKPGKAPK DASNLET GVPSRFSGSRS 240 33.22 VGDRVTITC LLIYGTDFTFTISSL QPEDIATYYC VK1- VK1-33 FL1 DIQMTQSPSSLSAS QASQDISNSLNWYQQKPGKAPK DASNLET GVPSRFSGSRS 241 33.23 VGDRVTITC LLIY GTDFTFTISSLQPEDIATYYC VK1- VK1-33 FL1 DIQMTQSPSSLSAS QASQDISNFLN WYQQKPGKAPKDASNLET GVPSRFSGSRS 242 33.24 VGDRVTITC LLIY GTDFTFTISSL QPEDIATYYC VK2-VK2-28 Germline DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSNRASGVPDRFSGSGS 243 28 PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2-VK2-28 L1 DIVMTQSPLSLPVT RSSQSLLYSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 24428.1 PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L1DIVMTQSPLSLPVT RSSQSLLHRN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 245 28.2PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L1DIVMTQSPLSLPVT RSSQSLLHTN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 246 28.3PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L1DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 247 28.4PGEPASISC GNNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L2DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LASNRAS GVPDRFSGSGS 248 28.5PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L2DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSHRAS GVPDRFSGSGS 249 28.6PGEPASISC GYNYLD LLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 FDIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 250 28.10PGEPASISC GYNYLD VLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 L1DIVMTQSPLSLPVT RSSQSLLYSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 251 28.11PGEPASISC GYNYLD VLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 FL2DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LASNRAS GVPDRFSGSGS 252 28.15PGEPASISC GYNYLD VLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 FL2DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSSRAS GVPDRFSGSGS 253 28.17PGEPASISC GYNYLD VLIY GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 FDIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 254 28.20PGEPASISC GYNYLD LLIF GTDFTLKISRV EAEDVGVYYC VK2- VK2-28 FL1DIVMTQSPLSLPVT RSSQSLLHSN WYLQKPGQSPQ LGSNRAS GVPDRFSGSGS 255 28.24PGEPASISC GNNYLD LLIF GTDFTLKISRV EAEDVGVYYC VK3- VK3-11 GermlineEIVLTQSPATLSLS RASQSVSSYLA WYQQKPGQAPR DASNRAT GIPARFSGSGS 256 11PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11 L1 EIVLTQSPATLSLSRASQSVSRYLA WYQQKPGQAPR DASNRAT GIPARFSGSGS 257 11.2 PGERATLSC LLIYGTDFTLTISSL EPEDFAVYYC VK3- VK3-11 L1 EIVLTQSPATLSLS RASQSVSNYLAWYQQKPGQAPR DASNRAT GIPARFSGSGS 258 11.3 PGERATLSC LLIY GTDFTLTISSLEPEDFAVYYC VK3- VK3-11 L2 EIVLTQSPATLSLS RASQSVSSYLA WYQQKPGQAPR DSSNRATGIPARFSGSGS 259 11.4 PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11L2 EIVLTQSPATLSLS RASQSVSSYLA WYQQKPGQAPR DTSNRAT GIPARFSGSGS 260 11.5PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11 L2 EIVLTQSPATLSLSRASQSVSSYLA WYQQKPGQAPR DASKRAT GIPARFSGSGS 261 11.6 PGERATLSC LLIYGTDFTLTISSL EPEDFAVYYC VK3- VK3-11 F EIVMTQSPATLSLS RASQSVSSYLAWYQQKPGQAPR DASNRAT GIPARFSGSGS 262 11.10 PGERATLSC LLIY GTDFTLTISSLEPEDFAVYYC VK3- VK3-11 FL1 EIVMTQSPATLSLS RASQSVSNYLA WYQQKPGQAPRDASNRAT GIPARFSGSGS 263 11.13 PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3-VK3-11 FL2 EIVMTQSPATLSLS RASQSVSSYLA WYQQKPGQAPR DSSNRAT GIPARFSGSGS264 11.14 PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11 FL2EIVMTQSPATLSLS RASQSVSSYLA WYQQKPGQAPR DTSNRAT GIPARFSGSGS 265 11.15PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11 FL2 EIVMTQSPATLSLSRASQSVSSYLA WYQQKPGQAPR DASKRAT GIPARFSGSGS 266 11.16 PGERATLSC LLIYGTDFTLTISSL EPEDFAVYYC VK3- VK3-11 F EIVLTQSPATLSLS RASQSVSSYLAWFQQKPGQAPR DASNRAT GIPARFSGSGS 267 11.20 PGERATLSC LLIY GTDFTLTISSLEPEDFAVYYC VK3- VK3-11 FL1 EIVLTQSPATLSLS RASQSISSYLA WFQQKPGQAPRDASNRAT GIPARFSGSGS 268 11.21 PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3-VK3-11 FL2 EIVLTQSPATLSLS RASQSVSSYLA WFQQKPGQAPR DSSNRAT GIPARFSGSGS269 11.24 PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-11 FL2EIVLTQSPATLSLS RASQSVSSYLA WFQQKPGQAPR DTSNRAT GIPARFSGSGS 270 11.25PGERATLSC LLIY GTDFTLTISSL EPEDFAVYYC VK3- VK3-15 GermlineEIVMTQSPATLSVS RASQSVSSNLA WYQQKPGQAPR GASTRAT GIPARFSGSGS 271 15PGERATLSC LLIY GTEFTLTISSL QSEDFAVYYC VK3- VK3-15 L1 EIVMTQSPATLSVSRASQSVGSNLA WYQQKPGQAPR GASTRAT GIPARFSGSGS 272 15.1 PGERATLSC LLIYGTEFTLTISSL QSEDFAVYYC VK3- VK3-15 L1 EIVMTQSPATLSVS RASQSVSSSLAWYQQKPGQAPR GASTRAT GIPARFSGSGS 273 15.6 PGERATLSC LLIY GTEFTLTISSLQSEDFAVYYC VK3- VK3-15 L2 EIVMTQSPATLSVS RASQSVSSNLA WYQQKPGQAPR DASTRATGIPARFSGSGS 274 15.7 PGERATLSC LLIY GTEFTLTISSL QSEDFAVYYC VK3- VK3-15L2 EIVMTQSPATLSVS RASQSVSSNLA WYQQKPGQAPR SASTRAT GIPARFSGSGS 275 15.8PGERATLSC LLIY GTEFTLTISSL QSEDFAVYYC VK3- VK3-15 F EIVLTQSPATLSVSRASQSVSSNLA WYQQKPGQAPR GASTRAT GIPARFSGSGS 276 15.10 PGERATLSC LLIYGTEFTLTISSL QSEDFAVYYC VK3- VK3-15 FL1 EIVLTQSPATLSVS RASQSVGSNLAWYQQKPGQAPR GASTRAT GIPARFSGSGS 277 15.11 PGERATLSC LLIY GTEFTLTISSLQSEDFAVYYC VK3- VK3-15 FL1 EIVLTQSPATLSVS RASQSVSTNLA WYQQKPGQAPRGASTRAT GIPARFSGSGS 278 15.14 PGERATLSC LLIY GTEFTLTISSL QSEDFAVYYC VK3-VK3-15 FL1 EIVLTQSPATLSVS RASQSVSSDLA WYQQKPGQAPR GASTRAT GIPARFSGSGS279 15.16 PGERATLSC LLIY GTEFTLTISSL QSEDFAVYYC VK3- VK3-15 FEIVMTQSPATLSVS RASQSVSSNLA WYQQKPGQAPR GASTRAT GIPARFSGSGS 280 15.20PGERATLSC LLIF GTEFTLTISSL QSEDFAVYYC VK3- VK3-15 FL1 EIVMTQSPATLSVSRASQSVGSNLA WYQQKPGQAPR GASTRAT GIPARFSGSGS 281 15.21 PGERATLSC LLIFGTEFTLTISSL QSEDFAVYYC VK3- VK3-15 FL1 EIVMTQSPATLSVS RASQSVSSDLAWYQQKPGQAPR GASTRAT GIPARFSGSGS 282 15.25 PGERATLSC LLIF GTEFTLTISSLQSEDFAVYYC VK3- VK3-15 FL1 EIVMTQSPATLSVS RASQSVSSSL WYQQKPGQAPR GASTRATGIPARFSGSGS 283 15.26 PGERATLSC LLIF GTEFTLTISSL QSEDFAVYYC VK3- VK3-20Germline EIVLTQSPGTLSLS RASQSVSSSY WYQQKPGQAPR GASSRAT GIPDRFSGSGS 28420 PGERATLSC LA LLIY GTDFTLTISRL EPEDFAVYYC VK3- VK3-20 L1EIVLTQSPGTLSLS RASQSVRSSY WYQQKPGQAPR GASSRAT GIPDRFSGSGS 285 20.1PGERATLSC LA LLIY GTDFTLTISRL EPEDFAVYYC VK3- VK3-20 L1 EIVLTQSPGTLSLSRASQSVSSDY WYQQKPGQAPR GASSRAT GIPDRFSGSGS 286 20.4 PGERATLSC LA LLIYGTDFTLTISRL EPEDFAVYYC VK3- VK3-20 L2 EIVLTQSPGTLSLS RASQSVSSSYWYQQKPGQAPR GASNRAT GIPDRFSGSGS 287 20.7 PGERATLSC LA LLIY GTDFTLTISRLEPEDFAVYYC VK3- VK3-20 L2 EIVLTQSPGTLSLS RASQSVSSSY WYQQKPGQAPR GASRRATGIPDRFSGSGS 288 20.8 PGERATLSC LA LLIY GTDFTLTISRL EPEDFAVYYC VK3-VK3-20 F EIVMTQSPGTLSLS RASQSVSSSY WYQQKPGQAPR GASSRAT GIPDRFSGSGS 28920.10 PGERATLSC LA LLIY GTDFTLTISRL EPEDFAVYYC VK3- VK3-20 FL2EIVMTQSPGTLSLS RASQSVSSSY WYQQKPGQAPR GASNRAT GIPDRFSGSGS 290 20.17PGERATLSC LA LLIY GTDFTLTISRL EPEDFAVYYC VK3- VK3-20 F EIVLTQSPGTLSLSRASQSVSSSY WYQQKPGQAPR GASSRAT GIPDRFSGSGS 291 20.20 PGERATLSC LA LLISGTDFTLTISRL EPEDFAVYYC VK3- VK3-20 FL1 EIVLTQSPGTLSLS RASQSVSSNYWYQQKPGQAPR GASSRAT GIPDRFSGSGS 292 20.22 PGERATLSC LA LLIS GTDFTLTISRLEPEDFAVYYC VK4- VK4-01 Germline DIVMTQSPDSLAVS KSSQSVLYSS WYQQKPGQPPKWASTRES GVPDRFSGSGS 293 01 LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYCVK4- VK4-01 L1 DIVMTQSPDSLAVS KSSQSLLYSS WYQQKPGQPPK WASTRES GVPDRFSGSGS294 01.1 LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 L1DIVMTQSPDSLAVS KSSQSILYSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 295 01.2LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 L1DIVMTQSPDSLAVS KSSQSVLHSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 296 01.3LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 L1DIVMTQSPDSLAVS KSSQSVLFSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 297 01.4LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 L1DIVMTQSPDSLAVS KSSQSVLYTS WYQQKPGQPPK WASTRES GVPDRFSGSGS 298 01.5LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 L2DIVMTQSPDSLAVS KSSQSVLYSS WYQQKPGQPPK WASSRES GVPDRFSGSGS 299 01.7LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 FDIVLTQSPDSLAVS KSSQSVLYSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 300 01.10LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 FL1DIVLTQSPDSLAVS KSSQSVLHSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 301 01.13LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 FL2DIVLTQSPDSLAVS KSSQSVLYSS WYQQKPGQPPK WASSRES GVPDRFSGSGS 302 01.17LGERATINC NNKNYLA LLIY GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 FDIVMTQSPDSLAVS KSSQSVLYSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 303 01.20LGERATINC NNKNYLA LLIS GTDFTLTISSL QAEDVAVYYC VK4- VK4-01 FL1DIVMTQSPDSLAVS KSSQSVLHSS WYQQKPGQPPK WASTRES GVPDRFSGSGS 304 01.23LGERATINC NNKNYLA LLIS GTDFTLTISSL QAEDVAVYYC

TABLE 4Jumping dimer and trimer oligonucleotides for the VK1-39 sequenceswith CDRL3 length nine and F as the junctional amino acid. i.e., Thesequences depicted below occur between YYC and FGG, to yield:...YYC-[89-97]-FGG... .The sequences in this table encompassed by positions “[89-97]”are disclosed as Name Oligo SEQ ID NO 89 90 91 92 93 94 95 96 97Jumping Dimer VK1-39_1 SWMSWMAGC 305 DEHLQV DEHLQV S Y S T P FY TTACAGTACT CCTTWCACT VK1-39_2 SWMCAAVNA 306 DEHLQV Q AEGIKLPQR Y S T P FYT TACAGTACT TV CCTTWCACT VK1-39_3 SWMCAAAGC 307 DEHLQV Q S ADFHLPS S T PFY T BHCAGTACT VY CCTTWCACT VK1-39_4 SWMCAAAGC 308 DEHLQV Q S Y ADFHILNPT P FY T TACNHCACT STVY CCTTWCACT VK1-39_5 SWMCAAAGC 309 DEHLQV Q S Y SADFHL P FY T TACAGTBHC PSVY CCTTWCACT VK1-39_6 CAGSWMVNA 310 Q DEHLQVAEGIKLPQR Y S T P FY T TACAGTACT TV CCTTWCACT VK1-39_7 CAGSWMAGC 311 QDEHLQV S ADFHLPS S T P FY T BHCAGTACT VY CCTTWCACT VK1-39_8 CAGSWMAGC312 Q DEHLQV S Y ADFHILNP T P FY T TACNHCACT STVY CCTTWCACT VK1-39_9CAGSWMAGC 313 Q DEHLQV S Y S ADFHL P FY T TACAGTBHC PSVY CCTTWCACTVK1-39_10 CAGCAAVNA 314 Q Q AEGIKLPQR ADFHLPS S T P FY T BHCAGTACT TV VYCCTTWCACT VK1-39_11 CAGCAAVNA 315 Q Q AEGIKLPQR Y ADFHILNP T P FY TTACNHCACT TV STVY CCTTWCACT VK1-39_12 CAGCAAVNA 316 Q Q AEGIKLPQR Y SADFHL P FY T TACAGTBHC TV PSVY CCTTWCACT VK1-39_13 CAGCAAAGC 317 Q Q SADFHLPS ADFHILNP T P FY T BHCNHCACT VY STVY CCTTWCACT VK1-39_14CAGCAAAGC 318 Q Q S ADFHLPS S ADFHL P FY T BHCAGTBHC VY PSVY CCTTWCACTVK1-39_15 CAGCAAAGC 319 Q Q S Y ADFHILNP ADFHL P FY T TACNHCBHC STVYPSVY CCTTWCACT Jumping Trimer VK1- CAGCAAVNA 314 Q Q AEGIKLPQR ADFHLPS ST P FY T 39_10_0_9 BHCAGTACT TV VY CCTTWCACT VK1- CAGCAAVNA 315 Q QAEGIKLPQR Y ADFHILNP T P FY T 39_11_0_9 TACNHCACT TV STVY CCTTWCACT VK1-CAGCAAVNA 316 Q Q AEGIKLPQR Y S ADFHL P FY T 39_12_0_9 TACAGTBHC TV PSVYCCTTWCACT VK1- CAGCAAAGC 317 Q Q S ADFHLPS ADFHILNP T P FY T 39_13_0_9BHCNHCACT VY STVY CCTTWCACT VK1- CAGCAAAGC 318 Q Q S ADFHLPS S ADFHL PFY T 39_14_0_9 BHCAGTBHC VY PSVY CCTTWCACT VK1- CAGCAAAGC 319 Q Q S YADFHILNP ADFHL P FY T 39_15_0_9 TACNHCBHC STVY PSVY CCTTWCACT VK1-CAGCAAVNA 320 Q Q AEGIKLPQR ADFHLPS ADFHILNP T P FY T 39_t1_0_9BHCNHCACT TV VY STVY CCTTWCACT VK1- CAGCAAVNA 321 Q Q AEGIKLPQR ADFHLPSS ADFHL P FY T 39_t2_0_9 BHCAGTBHC TV VY PSVY CCTTWCACT VK1- CAGCAAVNA322 Q Q AEGIKLPQR Y ADFHILNP ADFHL P FY T 39_t3_0_9 TACNHCBHC TV STVYPSVY CCTTWCACT VK1- CAGCAAAGC 323 Q Q S ADFHLPS ADFHILNP ADFHL P FY T39_t4_0_9 BHCNHCBHC VY STVY PSVY CCTTWCACT

TABLE 5Oligonucleotide sequences for exemplary VK jumping dimer and trimer sequenceswith CDRL3 length 8. Portion of Oligonucleotide SEQ ID SEQ ID NOSequence of Synthesized Corresponding to CDRL3 NO (CDRL3 NameOligonucleotide Proper (Oligo) Portion) Jumping Dimer VK1-05_1_0_8CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACA SWMSWMTACAATAGTTACTWCACT 324 948ATAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTTACTWCACT 325 94905_10_0_8 RMAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCTACTWCACT 326 95005_11_0_8 ATMBCTACTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATAGTYWCTWCACT 327 95105_12_0_8 ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCTACTWCACT 328 95205_13_0_8 RMMBCTACTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMAGTYWCTWCACT 329 95305_14_0_8 RMAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACA CAGCAGTACAATMBCYWCTWCACT 330 95405_15_0_8 ATMBCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-05_2_0_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCA SWMCAGBHCAATAGTTACTWCACT 331 955ATAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_3_0_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACV SWMCAGTACVRMAGTTACTWCACT 332 956RMAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_4_0_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATMBCTACTWCACT 333 957ATMBCTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_5_0_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATAGTYWCTWCACT 334 958ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-05_6_0_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCA CAGSWMBHCAATAGTTACTWCACT 335 959ATAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_7_0_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACV CAGSWMTACVRMAGTTACTWCACT 336 960RMAGTTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_8_0_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATMBCTACTWCACT 337 961ATMBCTACTWCACTTTTGGCGGAGGGACCAAG VK1-05_9_0_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATAGTYWCTWCACT 338 962ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-05_1_1_8CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACA SWMSWMTACAATAGTTACMTCACT 339 963ATAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTTACMTCACT 340 96405_10_1_8 RMAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCTACMTCACT 341 96505_11_1_8 ATMBCTACMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATAGTYWCMTCACT 342 96605_12_1_8 ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCTACMTCACT 343 96705_13_1_8 RMMBCTACMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMAGTYWCMTCACT 344 96805_14_1_8 RMAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACA CAGCAGTACAATMBCYWCMTCACT 345 96905_15_1_8 ATMBCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-05_2_1_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCA SWMCAGBHCAATAGTTACMTCACT 346 970ATAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_3_1_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACV SWMCAGTACVRMAGTTACMTCACT 347 971RMAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_4_1_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATMBCTACMTCACT 348 972ATMBCTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_5_1_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATAGTYWCMTCACT 349 973ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-05_6_1_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCA CAGSWMBHCAATAGTTACMTCACT 350 974ATAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_7_1_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACV CAGSWMTACVRMAGTTACMTCACT 351 975RMAGTTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_8_1_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATMBCTACMTCACT 352 976ATMBCTACMTCACTTTTGGCGGAGGGACCAAG VK1-05_9_1_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATAGTYWCMTCACT 353 977ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-05_1_2_8CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACA SWMSWMTACAATAGTTACWGGACT 354 978ATAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTTACWGGACT 355 97905_10_2_8 RMAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCTACWGGACT 356 98005_11_2_8 ATMBCTACWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATAGTYWCWGGACT 357 98105_12_2_8 ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCTACWGGACT 358 98205_13_2_8 RMMBCTACWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMAGTYWCWGGACT 359 98305_14_2_8 RMAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACA CAGCAGTACAATMBCYWCWGGACT 360 98405_15_2_8 ATMBCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-05_2_2_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCA SWMCAGBHCAATAGTTACWGGACT 361 985ATAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_3_2_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACV SWMCAGTACVRMAGTTACWGGACT 362 986RMAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_4_2_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATMBCTACWGGACT 363 987ATMBCTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_5_2_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATAGTYWCWGGACT 364 988ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-05_6_2_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCA CAGSWMBHCAATAGTTACWGGACT 365 989ATAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_7_2_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACV CAGSWMTACVRMAGTTACWGGACT 366 990RMAGTTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_8_2_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATMBCTACWGGACT 367 991ATMBCTACWGGACTTTTGGCGGAGGGACCAAG VK1-05_9_2_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATAGTYWCWGGACT 368 992ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-05_1_3_8CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACA SWMSWMTACAATAGTTACCCTACT 369 993ATAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTTACCCTACT 370 99405_10_3_8 RMAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCTACCCTACT 371 99505_11_3_8 ATMBCTACCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATAGTYWCCCTACT 372 99605_12_3_8 ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCTACCCTACT 373 99705_13_3_8 RMMBCTACCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMAGTYWCCCTACT 374 99805_14_3_8 RMAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACA CAGCAGTACAATMBCYWCCCTACT 375 99905_15_3_8 ATMBCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-05_2_3_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCA SWMCAGBHCAATAGTTACCCTACT 376 1000ATAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_3_3_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACV SWMCAGTACVRMAGTTACCCTACT 377 1001RMAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_4_3_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATMBCTACCCTACT 378 1002ATMBCTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_5_3_8CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACA SWMCAGTACAATAGTYWCCCTACT 379 1003ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-05_6_3_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCA CAGSWMBHCAATAGTTACCCTACT 380 1004ATAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_7_3_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACV CAGSWMTACVRMAGTTACCCTACT 381 1005RMAGTTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_8_3_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATMBCTACCCTACT 382 1006ATMBCTACCCTACTTTTGGCGGAGGGACCAAG VK1-05_9_3_8CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACA CAGSWMTACAATAGTYWCCCTACT 383 1007ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-12_1_0_8CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAA SWMSWMGCAAATAGTTTCTWCACT 384 1008ATAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTTTCTWCACT 385 100912_10_0_8 HCAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCTTCTWCACT 386 101012_11_0_8 ATNHCTTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATAGTYWCTWCACT 387 101112_12_0_8 ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCTTCTWCACT 388 101212_13_0_8 HCNHCTTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCAGTYWCTWCACT 389 101312_14_0_8 HCAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAA CAGCAGGCAAATNHCYWCTWCACT 390 101412_15_0_8 ATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-12_2_0_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAA SWMCAGRNAAATAGTTTCTWCACT 391 1015ATAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_3_0_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAN SWMCAGGCANHCAGTTTCTWCACT 392 1016HCAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_4_0_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATNHCTTCTWCACT 393 1017ATNHCTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_5_0_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATAGTYWCTWCACT 394 1018ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-12_6_0_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAA CAGSWMRNAAATAGTTTCTWCACT 395 1019ATAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_7_0_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAN CAGSWMGCANHCAGTTTCTWCACT 396 1020HCAGTTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_8_0_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATNHCTTCTWCACT 397 1021ATNHCTTCTWCACTTTTGGCGGAGGGACCAAG VK1-12_9_0_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATAGTYWCTWCACT 398 1022ATAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-12_1_1_8CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAA SWMSWMGCAAATAGTTTCMTCACT 399 1023ATAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTTTCMTCACT 400 102412_10_1_8 HCAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCTTCMTCACT 401 102512_11_1_8 ATNHCTTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATAGTYWCMTCACT 402 102612_12_1_8 ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCTTCMTCACT 403 102712_13_1_8 HCNHCTTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCAGTYWCMTCACT 404 102812_14_1_8 HCAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAA CAGCAGGCAAATNHCYWCMTCACT 405 102912_15_1_8 ATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-12_2_1_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAA SWMCAGRNAAATAGTTTCMTCACT 406 1030ATAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_3_1_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAN SWMCAGGCANHCAGTTTCMTCACT 407 1031HCAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_4_1_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATNHCTTCMTCACT 408 1032ATNHCTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_5_1_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATAGTYWCMTCACT 409 1033ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-12_6_1_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAA CAGSWMRNAAATAGTTTCMTCACT 410 1034ATAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_7_1_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAN CAGSWMGCANHCAGTTTCMTCACT 411 1035HCAGTTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_8_1_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATNHCTTCMTCACT 412 1036ATNHCTTCMTCACTTTTGGCGGAGGGACCAAG VK1-12_9_1_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATAGTYWCMTCACT 413 1037ATAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-12_1_2_8CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAA SWMSWMGCAAATAGTTTCWGGACT 414 1038ATAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTTTCWGGACT 415 103912_10_2_8 HCAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCTTCWGGACT 416 104012_11_2_8 ATNHCTTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATAGTYWCWGGACT 417 104112_12_2_8 ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCTTCWGGACT 418 104212_13_2_8 HCNHCTTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCAGTYWCWGGACT 419 104312_14_2_8 HCAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAA CAGCAGGCAAATNHCYWCWGGACT 420 104412_15_2_8 ATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-12_2_2_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAA SWMCAGRNAAATAGTTTCWGGACT 421 1045ATAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_3_2_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAN SWMCAGGCANHCAGTTTCWGGACT 422 1046HCAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_4_2_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATNHCTTCWGGACT 423 1047ATNHCTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_5_2_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATAGTYWCWGGACT 424 1048ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-12_6_2_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAA CAGSWMRNAAATAGTTTCWGGACT 425 1049ATAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_7_2_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAN CAGSWMGCANHCAGTTTCWGGACT 426 1050HCAGTTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_8_2_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATNHCTTCWGGACT 427 1051ATNHCTTCWGGACTTTTGGCGGAGGGACCAAG VK1-12_9_2_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATAGTYWCWGGACT 428 1052ATAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-12_1_3_8CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAA SWMSWMGCAAATAGTTTCCCTACT 429 1053ATAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTTTCCCTACT 430 105412_10_3_8 HCAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCTTCCCTACT 431 105512_11_3_8 ATNHCTTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATAGTYWCCCTACT 432 105612_12_3_8 ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCTTCCCTACT 433 105712_13_3_8 HCNHCTTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCAGTYWCCCTACT 434 105812_14_3_8 HCAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAA CAGCAGGCAAATNHCYWCCCTACT 435 105912_15_3_8 ATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-12_2_3_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAA SWMCAGRNAAATAGTTTCCCTACT 436 1060ATAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_3_3_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAN SWMCAGGCANHCAGTTTCCCTACT 437 1061HCAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_4_3_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATNHCTTCCCTACT 438 1062ATNHCTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_5_3_8CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAA SWMCAGGCAAATAGTYWCCCTACT 439 1063ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-12_6_3_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAA CAGSWMRNAAATAGTTTCCCTACT 440 1064ATAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_7_3_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAN CAGSWMGCANHCAGTTTCCCTACT 441 1065HCAGTTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_8_3_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATNHCTTCCCTACT 442 1066ATNHCTTCCCTACTTTTGGCGGAGGGACCAAG VK1-12_9_3_8CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAA CAGSWMGCAAATAGTYWCCCTACT 443 1067ATAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-33_1_0_8CCTGAAGATATTGCAACATATTACTGTSWMSWMTACG SWMSWMTACGATAATCTCTWCACT 444 1068ATAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATCTCTWCACT 445 106933_10_0_8 HCAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCCTCTWCACT 446 107033_11_0_8 ATNHCCTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATAATYWCTWCACT 447 107133_12_0_8 ATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCCTCTWCACT 448 107233_13_0_8 HCNHCCTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCAATYWCTWCACT 449 107333_14_0_8 HCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACG CAGCAGTACGATNHCYWCTWCACT 450 107433_15_0_8 ATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-33_2_0_8CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCG SWMCAGBHCGATAATCTCTWCACT 451 1075ATAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_3_0_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACN SWMCAGTACNHCAATCTCTWCACT 452 1076HCAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_4_0_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATNHCCTCTWCACT 453 1077ATNHCCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_5_0_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATAATYWCTWCACT 454 1078ATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK1-33_6_0_8CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCG CAGSWMBHCGATAATCTCTWCACT 455 1079ATAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_7_0_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACN CAGSWMTACNHCAATCTCTWCACT 456 1080HCAATCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_8_0_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATNHCCTCTWCACT 457 1081ATNHCCTCTWCACTTTTGGCGGAGGGACCAAG VK1-33_9_0_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATAATYWCTWCACT 458 1082ATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK1-33_1_1_8CCTGAAGATATTGCAACATATTACTGTSWMSWMTACG SWMSWMTACGATAATCTCMTCACT 459 1083ATAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATCTCMTCACT 460 108433_10_1_8 HCAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCCTCMTCACT 461 108533_11_1_8 ATNHCCTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATAATYWCMTCACT 462 108633_12_1_8 ATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCCTCMTCACT 463 108733_13_1_8 HCNHCCTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCAATYWCMTCACT 464 108833_14_1_8 HCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACG CAGCAGTACGATNHCYWCMTCACT 465 108933_15_1_8 ATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-33_2_1_8CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCG SWMCAGBHCGATAATCTCMTCACT 466 1090ATAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_3_1_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACN SWMCAGTACNHCAATCTCMTCACT 467 1091HCAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_4_1_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATNHCCTCMTCACT 468 1092ATNHCCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_5_1_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATAATYWCMTCACT 469 1093ATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK1-33_6_1_8CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCG CAGSWMBHCGATAATCTCMTCACT 470 1094ATAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_7_1_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACN CAGSWMTACNHCAATCTCMTCACT 471 1095HCAATCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_8_1_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATNHCCTCMTCACT 472 1096ATNHCCTCMTCACTTTTGGCGGAGGGACCAAG VK1-33_9_1_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATAATYWCMTCACT 473 1097ATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK1-33_1_2_8CCTGAAGATATTGCAACATATTACTGTSWMSWMTACG SWMSWMTACGATAATCTCWGGACT 474 1098ATAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATCTCWGGACT 475 109933_10_2_8 HCAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCCTCWGGACT 476 110033_11_2_8 ATNHCCTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATAATYWCWGGACT 477 110133_12_2_8 ATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCCTCWGGACT 478 110233_13_2_8 HCNHCCTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCAATYWCWGGACT 479 110333_14_2_8 HCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACG CAGCAGTACGATNHCYWCWGGACT 480 110433_15_2_8 ATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-33_2_2_8CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCG SWMCAGBHCGATAATCTCWGGACT 481 1105ATAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_3_2_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACN SWMCAGTACNHCAATCTCWGGACT 482 1106HCAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_4_2_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATNHCCTCWGGACT 483 1107ATNHCCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_5_2_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATAATYWCWGGACT 484 1108ATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK1-33_6_2_8CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCG CAGSWMBHCGATAATCTCWGGACT 485 1109ATAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_7_2_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACN CAGSWMTACNHCAATCTCWGGACT 486 1110HCAATCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_8_2_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATNHCCTCWGGACT 487 1111ATNHCCTCWGGACTTTTGGCGGAGGGACCAAG VK1-33_9_2_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATAATYWCWGGACT 488 1112ATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK1-33_1_3_8CCTGAAGATATTGCAACATATTACTGTSWMSWMTACG SWMSWMTACGATAATCTCCCTACT 489 1113ATAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATCTCCCTACT 490 111433_10_3_8 HCAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCCTCCCTACT 491 111533_11_3_8 ATNHCCTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATAATYWCCCTACT 492 111633_12_3_8 ATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCCTCCCTACT 493 111733_13_3_8 HCNHCCTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCAATYWCCCTACT 494 111833_14_3_8 HCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACG CAGCAGTACGATNHCYWCCCTACT 495 111933_15_3_8 ATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-33_2_3_8CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCG SWMCAGBHCGATAATCTCCCTACT 496 1120ATAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_3_3_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACN SWMCAGTACNHCAATCTCCCTACT 497 1121HCAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_4_3_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATNHCCTCCCTACT 498 1122ATNHCCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_5_3_8CCTGAAGATATTGCAACATATTACTGTSWMCAGTACG SWMCAGTACGATAATYWCCCTACT 499 1123ATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK1-33_6_3_8CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCG CAGSWMBHCGATAATCTCCCTACT 500 1124ATAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_7_3_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACN CAGSWMTACNHCAATCTCCCTACT 501 1125HCAATCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_8_3_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATNHCCTCCCTACT 502 1126ATNHCCTCCCTACTTTTGGCGGAGGGACCAAG VK1-33_9_3_8CCTGAAGATATTGCAACATATTACTGTCAGSWMTACG CAGSWMTACGATAATYWCCCTACT 503 1127ATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK1-39_1_0_8CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCT SWMSWMAGCTACAGTACTTWCACT 504 1128ACAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTACTTWCACT 505 112939_10_0_8 HCAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCACTTWCACT 506 113039_11_0_8 ACNHCACTTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACAGTBHCTWCACT 507 113139_12_0_8 ACAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCACTTWCACT 508 113239_13_0_8 HCNHCACTTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCAGTBHCTWCACT 509 113339_14_0_8 HCAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCT CAGCAAAGCTACNHCBHCTWCACT 510 113439_15_0_8 ACNHCBHCTWCACTTTTGGCGGAGGGACCAAG VK1-39_2_0_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNAT SWMCAAVNATACAGTACTTWCACT 511 1135ACAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_3_0_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCB SWMCAAAGCBHCAGTACTTWCACT 512 1136HCAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_4_0_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACNHCACTTWCACT 513 1137ACNHCACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_5_0_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACAGTBHCTWCACT 514 1138ACAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK1-39_6_0_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNAT CAGSWMVNATACAGTACTTWCACT 515 1139ACAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_7_0_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCB CAGSWMAGCBHCAGTACTTWCACT 516 1140HCAGTACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_8_0_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACNHCACTTWCACT 517 1141ACNHCACTTWCACTTTTGGCGGAGGGACCAAG VK1-39_9_0_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACAGTBHCTWCACT 518 1142ACAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK1-39_1_1_8CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCT SWMSWMAGCTACAGTACTMTCACT 519 1143ACAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTACTMTCACT 520 114439_10_1_8 HCAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCACTMTCACT 521 114539_11_1_8 ACNHCACTMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACAGTBHCMTCACT 522 114639_12_1_8 ACAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCACTMTCACT 523 114739_13_1_8 HCNHCACTMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCAGTBHCMTCACT 524 114839_14_1_8 HCAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCT CAGCAAAGCTACNHCBHCMTCACT 525 114939_15_1_8 ACNHCBHCMTCACTTTTGGCGGAGGGACCAAG VK1-39_2_1_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNAT SWMCAAVNATACAGTACTMTCACT 526 1150ACAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_3_1_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCB SWMCAAAGCBHCAGTACTMTCACT 527 1151HCAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_4_1_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACNHCACTMTCACT 528 1152ACNHCACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_5_1_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACAGTBHCMTCACT 529 1153ACAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK1-39_6_1_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNAT CAGSWMVNATACAGTACTMTCACT 530 1154ACAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_7_1_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCB CAGSWMAGCBHCAGTACTMTCACT 531 1155HCAGTACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_8_1_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACNHCACTMTCACT 532 1156ACNHCACTMTCACTTTTGGCGGAGGGACCAAG VK1-39_9_1_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACAGTBHCMTCACT 533 1157ACAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK1-39_1_2_8CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCT SWMSWMAGCTACAGTACTWGGACT 534 1158ACAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTACTWGGACT 535 115939_10_2_8 HCAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCACTWGGACT 536 116039_11_2_8 ACNHCACTWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACAGTBHCWGGACT 537 116139_12_2_8 ACAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCACTWGGACT 538 116239_13_2_8 HCNHCACTWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCAGTBHCWGGACT 539 116339_14_2_8 HCAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCT CAGCAAAGCTACNHCBHCWGGACT 540 116439_15_2_8 ACNHCBHCWGGACTTTTGGCGGAGGGACCAAG VK1-39_2_2_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNAT SWMCAAVNATACAGTACTWGGACT 541 1165ACAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_3_2_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCB SWMCAAAGCBHCAGTACTWGGACT 542 1166HCAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_4_2_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACNHCACTWGGACT 543 1167ACNHCACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_5_2_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACAGTBHCWGGACT 544 1168ACAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK1-39_6_2_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNAT CAGSWMVNATACAGTACTWGGACT 545 1169ACAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_7_2_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCB CAGSWMAGCBHCAGTACTWGGACT 546 1170HCAGTACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_8_2_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACNHCACTWGGACT 547 1171ACNHCACTWGGACTTTTGGCGGAGGGACCAAG VK1-39_9_2_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACAGTBHCWGGACT 548 1172ACAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK1-39_1_3_8CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCT SWMSWMAGCTACAGTACTCCTACT 549 1173ACAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTACTCCTACT 550 117439_10_3_8 HCAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCACTCCTACT 551 117539_11_3_8 ACNHCACTCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACAGTBHCCCTACT 552 117639_12_3_8 ACAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCACTCCTACT 553 117739_13_3_8 HCNHCACTCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCAGTBHCCCTACT 554 117839_14_3_8 HCAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCT CAGCAAAGCTACNHCBHCCCTACT 555 117939_15_3_8 ACNHCBHCCCTACTTTTGGCGGAGGGACCAAG VK1-39_2_3_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNAT SWMCAAVNATACAGTACTCCTACT 556 1180ACAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_3_3_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCB SWMCAAAGCBHCAGTACTCCTACT 557 1181HCAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_4_3_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACNHCACTCCTACT 558 1182ACNHCACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_5_3_8CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCT SWMCAAAGCTACAGTBHCCCTACT 559 1183ACAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK1-39_6_3_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNAT CAGSWMVNATACAGTACTCCTACT 560 1184ACAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_7_3_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCB CAGSWMAGCBHCAGTACTCCTACT 561 1185HCAGTACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_8_3_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACNHCACTCCTACT 562 1186ACNHCACTCCTACTTTTGGCGGAGGGACCAAG VK1-39_9_3_8CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCT CAGSWMAGCTACAGTBHCCCTACT 563 1187ACAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK2-28_1_0_8GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCAC DTSSWMGCACTCCAGACTTWCACT 564 1188TCCAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGACTTWCACT 565 118928_10_0_8 NACAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMACTTWCACT 566 119028_11_0_8 TCSRMACTTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCCAGVBCTWCACT 567 119128_12_0_8 TCCAGVBCTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMACTTWCACT 568 119228_13_0_8 NASRMACTTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNACAGVBCTWCACT 569 119328_14_0_8 NACAGVBCTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAC ATGCAGGCACTCSRMVBCTWCACT 570 119428_15_0_8 TCSRMVBCTWCACTTTTGGCGGAGGGACCAAG VK2-28_2_0_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNAC DTSCAGVNACTCCAGACTTWCACT 571 1195TCCAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_3_0_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAM DTSCAGGCAMNACAGACTTWCACT 572 1196NACAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_4_0_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCSRMACTTWCACT 573 1197TCSRMACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_5_0_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCCAGVBCTWCACT 574 1198TCCAGVBCTWCACTTTTGGCGGAGGGACCAAG VK2-28_6_0_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNAC ATGSWMVNACTCCAGACTTWCACT 575 1199TCCAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_7_0_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAM ATGSWMGCAMNACAGACTTWCACT 576 1200NACAGACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_8_0_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCSRMACTTWCACT 577 1201TCSRMACTTWCACTTTTGGCGGAGGGACCAAG VK2-28_9_0_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCCAGVBCTWCACT 578 1202TCCAGVBCTWCACTTTTGGCGGAGGGACCAAG VK2-28_1_1_8GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCAC DTSSWMGCACTCCAGACTMTCACT 579 1203TCCAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGACTMTCACT 580 120428_10_1_8 NACAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMACTMTCACT 581 120528_11_1_8 TCSRMACTMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCCAGVBCMTCACT 582 120628_12_1_8 TCCAGVBCMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMACTMTCACT 583 120728_13_1_8 NASRMACTMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNACAGVBCMTCACT 584 120828_14_1_8 NACAGVBCMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAC ATGCAGGCACTCSRMVBCMTCACT 585 120928_15_1_8 TCSRMVBCMTCACTTTTGGCGGAGGGACCAAG VK2-28_2_1_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNAC DTSCAGVNACTCCAGACTMTCACT 586 1210TCCAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_3_1_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAM DTSCAGGCAMNACAGACTMTCACT 587 1211NACAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_4_1_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCSRMACTMTCACT 588 1212TCSRMACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_5_1_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCCAGVBCMTCACT 589 1213TCCAGVBCMTCACTTTTGGCGGAGGGACCAAG VK2-28_6_1_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNAC ATGSWMVNACTCCAGACTMTCACT 590 1214TCCAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_7_1_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAM ATGSWMGCAMNACAGACTMTCACT 591 1215NACAGACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_8_1_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCSRMACTMTCACT 592 1216TCSRMACTMTCACTTTTGGCGGAGGGACCAAG VK2-28_9_1_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCCAGVBCMTCACT 593 1217TCCAGVBCMTCACTTTTGGCGGAGGGACCAAG VK2-28_1_2_8GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCAC DTSSWMGCACTCCAGACTWGGACT 594 1218TCCAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGACTWGGACT 595 121928_10_2_8 NACAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMACTWGGACT 596 122028_11_2_8 TCSRMACTWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCCAGVBCWGGACT 597 122128_12_2_8 TCCAGVBCWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMACTWGGACT 598 122228_13_2_8 NASRMACTWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNACAGVBCWGGACT 599 122328_14_2_8 NACAGVBCWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAC ATGCAGGCACTCSRMVBCWGGACT 600 122428_15_2_8 TCSRMVBCWGGACTTTTGGCGGAGGGACCAAG VK2-28_2_2_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNAC DTSCAGVNACTCCAGACTWGGACT 601 1225TCCAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_3_2_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAM DTSCAGGCAMNACAGACTWGGACT 602 1226NACAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_4_2_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCSRMACTWGGACT 603 1227TCSRMACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_5_2_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCCAGVBCWGGACT 604 1228TCCAGVBCWGGACTTTTGGCGGAGGGACCAAG VK2-28_6_2_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNAC ATGSWMVNACTCCAGACTWGGACT 605 1229TCCAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_7_2_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAM ATGSWMGCAMNACAGACTWGGACT 606 1230NACAGACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_8_2_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCSRMACTWGGACT 607 1231TCSRMACTWGGACTTTTGGCGGAGGGACCAAG VK2-28_9_2_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCCAGVBCWGGACT 608 1232TCCAGVBCWGGACTTTTGGCGGAGGGACCAAG VK2-28_1_3_8GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCAC DTSSWMGCACTCCAGACTCCTACT 609 1233TCCAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGACTCCTACT 610 123428_10_3_8 NACAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMACTCCTACT 611 123528_11_3_8 TCSRMACTCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCCAGVBCCCTACT 612 123628_12_3_8 TCCAGVBCCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMACTCCTACT 613 123728_13_3_8 NASRMACTCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNACAGVBCCCTACT 614 123828_14_3_8 NACAGVBCCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAC ATGCAGGCACTCSRMVBCCCTACT 615 123928_15_3_8 TCSRMVBCCCTACTTTTGGCGGAGGGACCAAG VK2-28_2_3_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNAC DTSCAGVNACTCCAGACTCCTACT 616 1240TCCAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_3_3_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAM DTSCAGGCAMNACAGACTCCTACT 617 1241NACAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_4_3_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCSRMACTCCTACT 618 1242TCSRMACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_5_3_8GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAC DTSCAGGCACTCCAGVBCCCTACT 619 1243TCCAGVBCCCTACTTTTGGCGGAGGGACCAAG VK2-28_6_3_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNAC ATGSWMVNACTCCAGACTCCTACT 620 1244TCCAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_7_3_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAM ATGSWMGCAMNACAGACTCCTACT 621 1245NACAGACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_8_3_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCSRMACTCCTACT 622 1246TCSRMACTCCTACTTTTGGCGGAGGGACCAAG VK2-28_9_3_8GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAC ATGSWMGCACTCCAGVBCCCTACT 623 1247TCCAGVBCCCTACTTTTGGCGGAGGGACCAAG VK3-11_1_0_8CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAA SWMSWMAGAAGTAATTGGTWCACT 624 1248GTAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATTGGTWCACT 625 124911_10_0_8 HCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCTGGTWCACT 626 125011_11_0_8 GTNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTAATYWCTWCACT 627 125111_12_0_8 GTAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCTGGTWCACT 628 125211_13_0_8 HCNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCAATYWCTWCACT 629 125311_14_0_8 HCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAA CAGCAGAGAAGTNHCYWCTWCACT 630 125411_15_0_8 GTNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-11_2_0_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCA SWMCAGBHCAGTAATTGGTWCACT 631 1255GTAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_3_0_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAN SWMCAGAGANHCAATTGGTWCACT 632 1256HCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_4_0_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTNHCTGGTWCACT 633 1257GTNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_5_0_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTAATYWCTWCACT 634 1258GTAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-11_6_0_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCA CAGSWMBHCAGTAATTGGTWCACT 635 1259GTAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_7_0_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAN CAGSWMAGANHCAATTGGTWCACT 636 1260HCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_8_0_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTNHCTGGTWCACT 637 1261GTNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-11_9_0_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTAATYWCTWCACT 638 1262GTAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-11_1_1_8CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAA SWMSWMAGAAGTAATTGGMTCACT 639 1263GTAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATTGGMTCACT 640 126411_10_1_8 HCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCTGGMTCACT 641 126511_11_1_8 GTNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTAATYWCMTCACT 642 126611_12_1_8 GTAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCTGGMTCACT 643 126711_13_1_8 HCNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCAATYWCMTCACT 644 126811_14_1_8 HCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAA CAGCAGAGAAGTNHCYWCMTCACT 645 126911_15_1_8 GTNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-11_2_1_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCA SWMCAGBHCAGTAATTGGMTCACT 646 1270GTAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_3_1_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAN SWMCAGAGANHCAATTGGMTCACT 647 1271HCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_4_1_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTNHCTGGMTCACT 648 1272GTNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_5_1_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTAATYWCMTCACT 649 1273GTAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-11_6_1_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCA CAGSWMBHCAGTAATTGGMTCACT 650 1274GTAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_7_1_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAN CAGSWMAGANHCAATTGGMTCACT 651 1275HCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_8_1_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTNHCTGGMTCACT 652 1276GTNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-11_9_1_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTAATYWCMTCACT 653 1277GTAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-11_1_2_8CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAA SWMSWMAGAAGTAATTGGWGGACT 654 1278GTAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATTGGWGGACT 655 127911_10_2_8 HCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCTGGWGGACT 656 128011_11_2_8 GTNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTAATYWCWGGACT 657 128111_12_2_8 GTAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCTGGWGGACT 658 128211_13_2_8 HCNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCAATYWCWGGACT 659 128311_14_2_8 HCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAA CAGCAGAGAAGTNHCYWCWGGACT 660 128411_15_2_8 GTNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-11_2_2_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCA SWMCAGBHCAGTAATTGGWGGACT 661 1285GTAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_3_2_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAN SWMCAGAGANHCAATTGGWGGACT 662 1286HCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_4_2_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTNHCTGGWGGACT 663 1287GTNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_5_2_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTAATYWCWGGACT 664 1288GTAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-11_6_2_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCA CAGSWMBHCAGTAATTGGWGGACT 665 1289GTAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_7_2_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAN CAGSWMAGANHCAATTGGWGGACT 666 1290HCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_8_2_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTNHCTGGWGGACT 667 1291GTNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-11_9_2_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTAATYWCWGGACT 668 1292GTAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-11_1_3_8CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAA SWMSWMAGAAGTAATTGGCCTACT 669 1293GTAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATTGGCCTACT 670 129411_10_3_8 HCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCTGGCCTACT 671 129511_11_3_8 GTNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTAATYWCCCTACT 672 129611_12_3_8 GTAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCTGGCCTACT 673 129711_13_3_8 HCNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCAATYWCCCTACT 674 129811_14_3_8 HCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAA CAGCAGAGAAGTNHCYWCCCTACT 675 129911_15_3_8 GTNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-11_2_3_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCA SWMCAGBHCAGTAATTGGCCTACT 676 1300GTAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_3_3_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAN SWMCAGAGANHCAATTGGCCTACT 677 1301HCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_4_3_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTNHCTGGCCTACT 678 1302GTNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_5_3_8CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAA SWMCAGAGAAGTAATYWCCCTACT 679 1303GTAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-11_6_3_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCA CAGSWMBHCAGTAATTGGCCTACT 680 1304GTAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_7_3_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAN CAGSWMAGANHCAATTGGCCTACT 681 1305HCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_8_3_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTNHCTGGCCTACT 682 1306GTNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-11_9_3_8CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAA CAGSWMAGAAGTAATYWCCCTACT 683 1307GTAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-15_1_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMT SWMSWMTACAATAATTGGTWCACT 684 1308ACAATAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATTGGTWCACT 685 124915_10_0_8 HCNHCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCTGGTWCACT 686 130915_11_0_8 HCAATNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATAATYWCTWCACT 687 131015_12_0_8 HCAATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCTGGTWCACT 688 131115_13_0_8 ACNHCNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCAATYWCTWCACT 689 107315_14_0_8 ACNHCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACAATNHCYWCTWCACT 690 131215_15_0_8 ACAATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-15_2_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGB SWMCAGBHCAATAATTGGTWCACT 691 1313HCAATAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_3_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACNHCAATTGGTWCACT 692 1314ACNHCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_4_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATNHCTGGTWCACT 693 1315ACAATNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_5_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATAATYWCTWCACT 694 1316ACAATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-15_6_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMB CAGSWMBHCAATAATTGGTWCACT 695 1317HCAATAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_7_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACNHCAATTGGTWCACT 696 1318ACNHCAATTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_8_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATNHCTGGTWCACT 697 1319ACAATNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-15_9_0_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATAATYWCTWCACT 698 1320ACAATAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-15_1_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMT SWMSWMTACAATAATTGGMTCACT 699 1321ACAATAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATTGGMTCACT 700 126415_10_1_8 HCNHCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCTGGMTCACT 701 132215_11_1_8 HCAATNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATAATYWCMTCACT 702 132315_12_1_8 HCAATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCTGGMTCACT 703 132415_13_1_8 ACNHCNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCAATYWCMTCACT 704 108815_14_1_8 ACNHCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACAATNHCYWCMTCACT 705 132515_15_1_8 ACAATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-15_2_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGB SWMCAGBHCAATAATTGGMTCACT 706 1326HCAATAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_3_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACNHCAATTGGMTCACT 707 1327ACNHCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_4_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATNHCTGGMTCACT 708 1328ACAATNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_5_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATAATYWCMTCACT 709 1329ACAATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-15_6_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMB CAGSWMBHCAATAATTGGMTCACT 710 1330HCAATAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_7_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACNHCAATTGGMTCACT 711 1331ACNHCAATTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_8_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATNHCTGGMTCACT 712 1332ACAATNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-15_9_1_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATAATYWCMTCACT 713 1333ACAATAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-15_1_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMT SWMSWMTACAATAATTGGWGGACT 714 1334ACAATAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATTGGWGGACT 715 127915_10_2_8 HCNHCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCTGGWGGACT 716 133515_11_2_8 HCAATNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATAATYWCWGGACT 717 133615_12_2_8 HCAATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCTGGWGGACT 718 133715_13_2_8 ACNHCNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCAATYWCWGGACT 719 110315_14_2_8 ACNHCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACAATNHCYWCWGGACT 720 133815_15_2_8 ACAATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-15_2_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGB SWMCAGBHCAATAATTGGWGGACT 721 1339HCAATAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_3_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACNHCAATTGGWGGACT 722 1340ACNHCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_4_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATNHCTGGWGGACT 723 1341ACAATNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_5_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATAATYWCWGGACT 724 1342ACAATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-15_6_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMB CAGSWMBHCAATAATTGGWGGACT 725 1343HCAATAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_7_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACNHCAATTGGWGGACT 726 1344ACNHCAATTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_8_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATNHCTGGWGGACT 727 1345ACAATNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-15_9_2_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATAATYWCWGGACT 728 1346ACAATAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-15_1_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMT SWMSWMTACAATAATTGGCCTACT 729 1347ACAATAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATTGGCCTACT 730 129415_10_3_8 HCNHCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCTGGCCTACT 731 134815_11_3_8 HCAATNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATAATYWCCCTACT 732 134915_12_3_8 HCAATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCTGGCCTACT 733 135015_13_3_8 ACNHCNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCAATYWCCCTACT 734 111815_14_3_8 ACNHCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACAATNHCYWCCCTACT 735 135115_15_3_8 ACAATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-15_2_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGB SWMCAGBHCAATAATTGGCCTACT 736 1352HCAATAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_3_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACNHCAATTGGCCTACT 737 1353ACNHCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_4_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATNHCTGGCCTACT 738 1354ACAATNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_5_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGT SWMCAGTACAATAATYWCCCTACT 739 1355ACAATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-15_6_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMB CAGSWMBHCAATAATTGGCCTACT 740 1356HCAATAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_7_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACNHCAATTGGCCTACT 741 1357ACNHCAATTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_8_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATNHCTGGCCTACT 742 1358ACAATNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-15_9_3_8CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMT CAGSWMTACAATAATYWCCCTACT 743 1359ACAATAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-20_1_0_8CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACG SWMSWMTACGGAAGTAGTTWCACT 744 1360GAAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTAGTTWCACT 745 136120_10_0_8 HCAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCAGTTWCACT 746 136220_11_0_8 GAVNCAGTTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAAGTBHCTWCACT 747 136320_12_0_8 GAAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCAGTTWCACT 748 136420_13_0_8 HCVNCAGTTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCAGTBHCTWCACT 749 136520_14_0_8 HCAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACG CAGCAGTACGGAVNCBHCTWCACT 750 136620_15_0_8 GAVNCBHCTWCACTTTTGGCGGAGGGACCAAG VK3-20_2_0_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCG SWMCAGBHCGGAAGTAGTTWCACT 751 1367GAAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_3_0_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACB SWMCAGTACBHCAGTAGTTWCACT 752 1368HCAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_4_0_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAVNCAGTTWCACT 753 1369GAVNCAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_5_0_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAAGTBHCTWCACT 754 1370GAAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK3-20_6_0_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCG CAGSWMBHCGGAAGTAGTTWCACT 755 1371GAAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_7_0_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACB CAGSWMTACBHCAGTAGTTWCACT 756 1372HCAGTAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_8_0_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAVNCAGTTWCACT 757 1373GAVNCAGTTWCACTTTTGGCGGAGGGACCAAG VK3-20_9_0_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAAGTBHCTWCACT 758 1374GAAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK3-20_1_1_8CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACG SWMSWMTACGGAAGTAGTMTCACT 759 1375GAAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTAGTMTCACT 760 137620_10_1_8 HCAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCAGTMTCACT 761 137720_11_1_8 GAVNCAGTMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAAGTBHCMTCACT 762 137820_12_1_8 GAAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCAGTMTCACT 763 137920_13_1_8 HCVNCAGTMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCAGTBHCMTCACT 764 138020_14_1_8 HCAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACG CAGCAGTACGGAVNCBHCMTCACT 765 138120_15_1_8 GAVNCBHCMTCACTTTTGGCGGAGGGACCAAG VK3-20_2_1_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCG SWMCAGBHCGGAAGTAGTMTCACT 766 1382GAAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_3_1_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACB SWMCAGTACBHCAGTAGTMTCACT 767 1383HCAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_4_1_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAVNCAGTMTCACT 768 1384GAVNCAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_5_1_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAAGTBHCMTCACT 769 1385GAAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK3-20_6_1_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCG CAGSWMBHCGGAAGTAGTMTCACT 770 1386GAAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_7_1_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACB CAGSWMTACBHCAGTAGTMTCACT 771 1387HCAGTAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_8_1_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAVNCAGTMTCACT 772 1388GAVNCAGTMTCACTTTTGGCGGAGGGACCAAG VK3-20_9_1_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAAGTBHCMTCACT 773 1389GAAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK3-20_1_2_8CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACG SWMSWMTACGGAAGTAGTWGGACT 774 1390GAAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTAGTWGGACT 775 139120_10_2_8 HCAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCAGTWGGACT 776 139220_11_2_8 GAVNCAGTWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAAGTBHCWGGACT 777 139320_12_2_8 GAAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCAGTWGGACT 778 139420_13_2_8 HCVNCAGTWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCAGTBHCWGGACT 779 139520_14_2_8 HCAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACG CAGCAGTACGGAVNCBHCWGGACT 780 139620_15_2_8 GAVNCBHCWGGACTTTTGGCGGAGGGACCAAG VK3-20_2_2_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCG SWMCAGBHCGGAAGTAGTWGGACT 781 1397GAAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_3_2_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACB SWMCAGTACBHCAGTAGTWGGACT 782 1398HCAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_4_2_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAVNCAGTWGGACT 783 1399GAVNCAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_5_2_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAAGTBHCWGGACT 784 1400GAAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK3-20_6_2_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCG CAGSWMBHCGGAAGTAGTWGGACT 785 1401GAAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_7_2_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACB CAGSWMTACBHCAGTAGTWGGACT 786 1402HCAGTAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_8_2_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAVNCAGTWGGACT 787 1403GAVNCAGTWGGACTTTTGGCGGAGGGACCAAG VK3-20_9_2_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAAGTBHCWGGACT 788 1404GAAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK3-20_1_3_8CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACG SWMSWMTACGGAAGTAGTCCTACT 789 1405GAAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTAGTCCTACT 790 140620_10_3_8 HCAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCAGTCCTACT 791 140720_11_3_8 GAVNCAGTCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAAGTBHCCCTACT 792 140820_12_3_8 GAAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCAGTCCTACT 793 140920_13_3_8 HCVNCAGTCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCAGTBHCCCTACT 794 141020_14_3_8 HCAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACG CAGCAGTACGGAVNCBHCCCTACT 795 141120_15_3_8 GAVNCBHCCCTACTTTTGGCGGAGGGACCAAG VK3-20_2_3_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCG SWMCAGBHCGGAAGTAGTCCTACT 796 1412GAAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_3_3_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACB SWMCAGTACBHCAGTAGTCCTACT 797 1413HCAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_4_3_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAVNCAGTCCTACT 798 1414GAVNCAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_5_3_8CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACG SWMCAGTACGGAAGTBHCCCTACT 799 1415GAAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK3-20_6_3_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCG CAGSWMBHCGGAAGTAGTCCTACT 800 1416GAAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_7_3_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACB CAGSWMTACBHCAGTAGTCCTACT 801 1417HCAGTAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_8_3_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAVNCAGTCCTACT 802 1418GAVNCAGTCCTACTTTTGGCGGAGGGACCAAG VK3-20_9_3_8CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACG CAGSWMTACGGAAGTBHCCCTACT 803 1419GAAGTBHCCCTACTTTTGGCGGAGGGACCAAG Jumping Trimer VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMMBCTACTWCACT 804 142005_t1_0_8 RMMBCTACTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMMBCTACMTCACT 805 142105_t1_1_8 RMMBCTACMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMMBCTACWGGACT 806 142205_t1_2_8 RMMBCTACWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMMBCTACYCTACT 807 142305_t1_3_8 RMMBCTACYCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCYWCTWCACT 808 142405_t2_0_8 ATMBCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCYWCMTCACT 809 142505_t2_1_8 ATMBCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCYWCWGGACT 810 142605_t2_2_8 ATMBCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCA CAGCAGBHCAATMBCYWCYCTACT 811 142705_t2_3_8 ATMBCYWCYCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTYWCTWCACT 812 142805_t3_0_8 RMAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTYWCMTCACT 813 142905_t3_1_8 RMAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTYWCWGGACT 814 143005_t3_2_8 RMAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCV CAGCAGBHCVRMAGTYWCYCTACT 815 143105_t3_3_8 RMAGTYWCYCTACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCYWCTWCACT 816 143205_t4_0_8 RMMBCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCYWCMTCACT 817 143305_t4_1_8 RMMBCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCYWCWGGACT 818 143405_t4_2_8 RMMBCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACV CAGCAGTACVRMMBCYWCYCTACT 819 143505_t4_3_8 RMMBCYWCYCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCNHCTTCTWCACT 820 143612_t1_0_8 HCNHCTTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCNHCTTCMTCACT 821 143712_t1_1_8 HCNHCTTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCNHCTTCWGGACT 822 143812_t1_2_8 HCNHCTTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCNHCTTCCCTACT 823 143912_t1_3_8 HCNHCTTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTYWCTWCACT 824 144012_t2_0_8 HCAGTYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTYWCMTCACT 825 144112_t2_1_8 HCAGTYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTYWCWGGACT 826 144212_t2_2_8 HCAGTYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAN CAGCAGRNANHCAGTYWCCCTACT 827 144312_t2_3_8 HCAGTYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCYWCTWCACT 828 144412_t3_0_8 ATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCYWCMTCACT 829 144512_t3_1_8 ATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCYWCWGGACT 830 144612_t3_2_8 ATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAA CAGCAGRNAAATNHCYWCCCTACT 831 144712_t3_3_8 ATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCYWCTWCACT 832 144812_t4_0_8 HCNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCYWCMTCACT 833 144912_t4_1_8 HCNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCYWCWGGACT 834 145012_t4_2_8 HCNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAN CAGCAGGCANHCNHCYWCCCTACT 835 145112_t4_3_8 HCNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCCTCTWCACT 836 145233_t1_0_8 HCNHCCTCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCCTCMTCACT 837 145333_t1_1_8 HCNHCCTCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCCTCWGGACT 838 145433_t1_2_8 HCNHCCTCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCCTCCCTACT 839 145533_t1_3_8 HCNHCCTCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCTWCACT 840 145633_t2_0_8 HCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCMTCACT 841 145733_t2_1_8 HCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCWGGACT 842 145833_t2_2_8 HCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCCCTACT 843 145933_t2_3_8 HCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCYWCTWCACT 844 146033_t3_0_8 ATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCYWCMTCACT 845 146133_t3_1_8 ATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCYWCWGGACT 846 146233_t3_2_8 ATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCG CAGCAGBHCGATNHCYWCCCTACT 847 146333_t3_3_8 ATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCYWCTWCACT 848 146433_t4_0_8 HCNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCYWCMTCACT 849 146533_t4_1_8 HCNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCYWCWGGACT 850 146633_t4_2_8 HCNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACN CAGCAGTACNHCNHCYWCCCTACT 851 146733_t4_3_8 HCNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCNHCACTTWCACT 852 146839_t1_0_8 HCNHCACTTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCNHCACTMTCACT 853 146939_t1_1_8 HCNHCACTMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCNHCACTWGGACT 854 147039_t1_2_8 HCNHCACTWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCNHCACTCCTACT 855 147139_t1_3_8 HCNHCACTCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTBHCTWCACT 856 147239_t2_0_8 HCAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTBHCMTCACT 857 147339_t2_1_8 HCAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTBHCWGGACT 858 147439_t2_2_8 HCAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAB CAGCAAVNABHCAGTBHCCCTACT 859 147539_t2_3_8 HCAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCBHCTWCACT 860 147639_t3_0_8 ACNHCBHCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCBHCMTCACT 861 147739_t3_1_8 ACNHCBHCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCBHCWGGACT 862 147839_t3_2_8 ACNHCBHCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNAT CAGCAAVNATACNHCBHCCCTACT 863 147939_t3_3_8 ACNHCBHCCCTACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCBHCTWCACT 864 148039_t4_0_8 HCNHCBHCTWCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCBHCMTCACT 865 148139_t4_1_8 HCNHCBHCMTCACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCBHCWGGACT 866 148239_t4_2_8 HCNHCBHCWGGACTTTTGGCGGAGGGACCAAG VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCB CAGCAAAGCBHCNHCBHCCCTACT 867 148339_t4_3_8 HCNHCBHCCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNASRMACTTWCACT 868 148428_t1_0_8 NASRMACTTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNASRMACTMTCACT 869 148528_t1_1_8 NASRMACTMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNASRMACTWGGACT 870 148628_t1_2_8 NASRMACTWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNASRMACTCCTACT 871 148728_t1_3_8 NASRMACTCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGVBCTWCACT 872 148828_t2_0_8 NACAGVBCTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGVBCMTCACT 873 148928_t2_1_8 NACAGVBCMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGVBCWGGACT 874 149028_t2_2_8 NACAGVBCWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAM ATGCAGVNAMNACAGVBCCCTACT 875 149128_t2_3_8 NACAGVBCCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMVBCTWCACT 876 149228_t3_0_8 TCSRMVBCTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMVBCMTCACT 877 149328_t3_1_8 TCSRMVBCMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMVBCWGGACT 878 149428_t3_2_8 TCSRMVBCWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAC ATGCAGVNACTCSRMVBCCCTACT 879 149528_t3_3_8 TCSRMVBCCCTACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMVBCTWCACT 880 149628_t4_0_8 NASRMVBCTWCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMVBCMTCACT 881 149728_t4_1_8 NASRMVBCMTCACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMVBCWGGACT 882 149828_t4_2_8 NASRMVBCWGGACTTTTGGCGGAGGGACCAAG VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAM ATGCAGGCAMNASRMVBCCCTACT 883 149928_t4_3_8 NASRMVBCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCTGGTWCACT 884 150011_t1_0_8 HCNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCTGGMTCACT 885 150111_t1_1_8 HCNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCTGGWGGACT 886 150211_t1_2_8 HCNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCNHCTGGCCTACT 887 150311_t1_3_8 HCNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCTWCACT 888 145611_t2_0_8 HCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCMTCACT 889 145711_t2_1_8 HCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCWGGACT 890 145811_t2_2_8 HCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCN CAGCAGBHCNHCAATYWCCCTACT 891 145911_t2_3_8 HCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCYWCTWCACT 892 150411_t3_0_8 GTNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCYWCMTCACT 893 150511_t3_1_8 GTNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCYWCWGGACT 894 150611_t3_2_8 GTNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCA CAGCAGBHCAGTNHCYWCCCTACT 895 150711_t3_3_8 GTNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCYWCTWCACT 896 150811_t4_0_8 HCNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCYWCMTCACT 897 150911_t4_1_8 HCNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCYWCWGGACT 898 151011_t4_2_8 HCNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAN CAGCAGAGANHCNHCYWCCCTACT 899 151111_t4_3_8 HCNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCNHCTGGTWCACT 900 150015_t1_0_8 HCNHCNHCTGGTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCNHCTGGMTCACT 901 150115_t1_1_8 HCNHCNHCTGGMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCNHCTGGWGGACT 902 150215_t1_2_8 HCNHCNHCTGGWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCNHCTGGCCTACT 903 150315_t1_3_8 HCNHCNHCTGGCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATYWCTWCACT 904 145615_t2_0_8 HCNHCAATYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATYWCMTCACT 905 145715_t2_1_8 HCNHCAATYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATYWCWGGACT 906 145815_t2_2_8 HCNHCAATYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCNHCAATYWCCCTACT 907 145915_t2_3_8 HCNHCAATYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCYWCTWCACT 908 151215_t3_0_8 HCAATNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCYWCMTCACT 909 151315_t3_1_8 HCAATNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCYWCWGGACT 910 151415_t3_2_8 HCAATNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGB CAGCAGBHCAATNHCYWCCCTACT 911 151515_t3_3_8 HCAATNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCYWCTWCACT 912 146415_t4_0_8 ACNHCNHCYWCTWCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCYWCMTCACT 913 146515_t4_1_8 ACNHCNHCYWCMTCACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCYWCWGGACT 914 146615_t4_2_8 ACNHCNHCYWCWGGACTTTTGGCGGAGGGACCAAG VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGT CAGCAGTACNHCNHCYWCCCTACT 915 146715_t4_3_8 ACNHCNHCYWCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCVNCAGTTWCACT 916 151620_t1_0_8 HCVNCAGTTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCVNCAGTMTCACT 917 151720_t1_1_8 HCVNCAGTMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCVNCAGTWGGACT 918 151820_t1_2_8 HCVNCAGTWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCVNCAGTCCTACT 919 151920_t1_3_8 HCVNCAGTCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCTWCACT 920 152020_t2_0_8 HCAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCMTCACT 921 152120_t2_1_8 HCAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCWGGACT 922 152220_t2_2_8 HCAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCCCTACT 923 152320_t2_3_8 HCAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCBHCTWCACT 924 152420_t3_0_8 GAVNCBHCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCBHCMTCACT 925 152520_t3_1_8 GAVNCBHCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCBHCWGGACT 926 152620_t3_2_8 GAVNCBHCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCG CAGCAGBHCGGAVNCBHCCCTACT 927 152720_t3_3_8 GAVNCBHCCCTACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCBHCTWCACT 928 152820_t4_0_8 HCVNCBHCTWCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCBHCMTCACT 929 152920_t4_1_8 HCVNCBHCMTCACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCBHCWGGACT 930 153020_t4_2_8 HCVNCBHCWGGACTTTTGGCGGAGGGACCAAG VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACB CAGCAGTACBHCVNCBHCCCTACT 931 153120_t4_3_8 HCVNCBHCCCTACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCNHCACTTWCACT 932 153201_t1_0_8 HCNHCACTTWCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCNHCACTMTCACT 933 153301_t1_1_8 HCNHCACTMTCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCNHCACTWGGACT 934 153401_t1_2_8 HCNHCACTWGGACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCNHCACTCCTACT 935 153501_t1_3_8 HCNHCACTCCTACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCTWCACT 936 152001_t2_0_8 HCAGTBHCTWCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCMTCACT 937 152101_t2_1_8 HCAGTBHCMTCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCWGGACT 938 152201_t2_2_8 HCAGTBHCWGGACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCB CAGCAGBHCBHCAGTBHCCCTACT 939 152301_t2_3_8 HCAGTBHCCCTACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCT CAGCAGBHCTACNHCBHCTWCACT 940 153601_t3_0_8 ACNHCBHCTWCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCT CAGCAGBHCTACNHCBHCMTCACT 941 153701_t3_1_8 ACNHCBHCMTCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCT CAGCAGBHCTACNHCBHCWGGACT 942 153801_t3_2_8 ACNHCBHCWGGACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCT CAGCAGBHCTACNHCBHCCCTACT 943 153901_t3_3_8 ACNHCBHCCCTACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACB CAGCAGTACBHCNHCBHCTWCACT 944 154001_t4_0_8 HCNHCBHCTWCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACB CAGCAGTACBHCNHCBHCMTCACT 945 154101_t4_1_8 HCNHCBHCMTCACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACB CAGCAGTACBHCNHCBHCWGGACT 946 154201_t4_2_8 HCNHCBHCWGGACTTTTGGCGGAGGGACCAAG VK4-GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACB CAGCAGTACBHCNHCBHCCCTACT 947 154301_t4_3_8 HCNHCBHCCCTACTTTTGGCGGAGGGACCAAG

TABLE 6 Oligonucleotide sequences for exemplary VK jumping dimer andtrimer sequences with CDRL3 length 9. Portion of SEQ ID OligonucleotideSEQ ID NO Corresponding to CDRL3 NO (CDRL3 Name Sequence of SynthesizedOligonucleotide Proper (Oligo) Portion) Jumping Dimer VK1-05_1_0_9CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAG SWMSWMTACAATAGTTACYCTT 15442168 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAG CAGCAGBHCVRMAGTTACYCTT 15452169 05_10_0_9 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMB CAGCAGBHCAATMBCTACYCTT 15462170 05_11_0_9 CTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAG CAGCAGBHCAATAGTYWCYCTT 15472171 05_12_0_9 TYWCYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMB CAGCAGTACVRMMBCTACYCTT 15482172 05_13_0_9 CTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAG CAGCAGTACVRMAGTYWCYCTT 15492173 05_14_0_9 TYWCYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMB CAGCAGTACAATMBCYWCYCTT 15502174 05_15_0_9 CYWCYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_2_0_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAG SWMCAGBHCAATAGTTACYCTT 15512175 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_3_0_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAG SWMCAGTACVRMAGTTACYCTT 15522176 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_4_0_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMB SWMCAGTACAATMBCTACYCTT 15532177 CTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_5_0_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAG SWMCAGTACAATAGTYWCYCTT 15542178 TYWCYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_6_0_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAG CAGSWMBHCAATAGTTACYCTT 15552179 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_7_0_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAG CAGSWMTACVRMAGTTACYCTT 15562180 TTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_8_0_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMB CAGSWMTACAATMBCTACYCTT 15572181 CTACYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_9_0_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAG CAGSWMTACAATAGTYWCYCTT 15582182 TYWCYCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-05_1_1_9CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAG SWMSWMTACAATAGTTACYCTM 15592183 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAG CAGCAGBHCVRMAGTTACYCTM 15602184 05_10_1_9 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMB CAGCAGBHCAATMBCTACYCTM 15612185 05_11_1_9 CTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAG CAGCAGBHCAATAGTYWCYCTM 15622186 05_12_1_9 TYWCYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMB CAGCAGTACVRMMBCTACYCTM 15632187 05_13_1_9 CTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAG CAGCAGTACVRMAGTYWCYCTM 15642188 05_14_1_9 TYWCYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMB CAGCAGTACAATMBCYWCYCTM 15652189 05_15_1_9 CYWCYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_2_1_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAG SWMCAGBHCAATAGTTACYCTM 15662190 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_3_1_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAG SWMCAGTACVRMAGTTACYCTM 15672191 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_4_1_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMB SWMCAGTACAATMBCTACYCTM 15682192 CTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_5_1_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAG SWMCAGTACAATAGTYWCYCTM 15692193 TYWCYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_6_1_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAG CAGSWMBHCAATAGTTACYCTM 15702194 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_7_1_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAG CAGSWMTACVRMAGTTACYCTM 15712195 TTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_8_1_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMB CAGSWMTACAATMBCTACYCTM 15722196 CTACYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_9_1_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAG CAGSWMTACAATAGTYWCYCTM 15732197 TYWCYCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-05_1_2_9CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAG SWMSWMTACAATAGTTACYCTW 15742198 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAG CAGCAGBHCVRMAGTTACYCTW 15752199 05_10_2_9 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMB CAGCAGBHCAATMBCTACYCTW 15762200 05_11_2_9 CTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAG CAGCAGBHCAATAGTYWCYCTW 15772201 05_12_2_9 TYWCYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMB CAGCAGTACVRMMBCTACYCTW 15782202 05_13_2_9 CTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAG CAGCAGTACVRMAGTYWCYCTW 15792203 05_14_2_9 TYWCYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMB CAGCAGTACAATMBCYWCYCTW 15802204 05_15_2_9 CYWCYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_2_2_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAG SWMCAGBHCAATAGTTACYCTW 15812205 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_3_2_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAG SWMCAGTACVRMAGTTACYCTW 15822206 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_4_2_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMB SWMCAGTACAATMBCTACYCTW 15832207 CTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_5_2_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAG SWMCAGTACAATAGTYWCYCTW 15842208 TYWCYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_6_2_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAG CAGSWMBHCAATAGTTACYCTW 15852209 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_7_2_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAG CAGSWMTACVRMAGTTACYCTW 15862210 TTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_8_2_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMB CAGSWMTACAATMBCTACYCTW 15872211 CTACYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_9_2_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAG CAGSWMTACAATAGTYWCYCTW 15882212 TYWCYCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-05_1_3_9CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAG SWMSWMTACAATAGTTACYCTC 15892213 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAG CAGCAGBHCVRMAGTTACYCTC 15902214 05_10_3_9 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMB CAGCAGBHCAATMBCTACYCTC 15912215 05_11_3_9 CTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAG CAGCAGBHCAATAGTYWCYCTC 15922216 05_12_3_9 TYWCYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMB CAGCAGTACVRMMBCTACYCTC 15932217 05_13_3_9 CTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAG CAGCAGTACVRMAGTYWCYCTC 15942218 05_14_3_9 TYWCYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMB CAGCAGTACAATMBCYWCYCTC 15952219 05_15_3_9 CYWCYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_2_3_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAG SWMCAGBHCAATAGTTACYCTC 15962220 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_3_3_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAG SWMCAGTACVRMAGTTACYCTC 15972221 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_4_3_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMB SWMCAGTACAATMBCTACYCTC 15982222 CTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_5_3_9CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAG SWMCAGTACAATAGTYWCYCTC 15992223 TYWCYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_6_3_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAG CAGSWMBHCAATAGTTACYCTC 16002224 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_7_3_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAG CAGSWMTACVRMAGTTACYCTC 16012225 TTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_8_3_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMB CAGSWMTACAATMBCTACYCTC 16022226 CTACYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-05_9_3_9CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAG CAGSWMTACAATAGTYWCYCTC 16032227 TYWCYCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_1_0_9CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAG SWMSWMGCAAATAGTTTCCCTT 16042228 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAG CAGCAGRNANHCAGTTTCCCTT 16052229 12_10_0_9 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNH CAGCAGRNAAATNHCTTCCCTT 16062230 12_11_0_9 CTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAG CAGCAGRNAAATAGTYWCCCTT 16072231 12_12_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNH CAGCAGGCANHCNHCTTCCCTT 16082232 12_13_0_9 CTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAG CAGCAGGCANHCAGTYWCCCTT 16092233 12_14_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNH CAGCAGGCAAATNHCYWCCCTT 16102234 12_15_0_9 CYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_2_0_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAG SWMCAGRNAAATAGTTTCCCTT 16112235 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_3_0_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAG SWMCAGGCANHCAGTTTCCCTT 16122236 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_4_0_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNH SWMCAGGCAAATNHCTTCCCTT 16132237 CTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_5_0_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAG SWMCAGGCAAATAGTYWCCCTT 16142238 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_6_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAG CAGSWMRNAAATAGTTTCCCTT 16152239 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_7_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAG CAGSWMGCANHCAGTTTCCCTT 16162240 TTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_8_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNH CAGSWMGCAAATNHCTTCCCTT 16172241 CTTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_9_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAG CAGSWMGCAAATAGTYWCCCTT 16182242 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-12_1_1_9CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAG SWMSWMGCAAATAGTTTCCCTM 16192243 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAG CAGCAGRNANHCAGTTTCCCTM 16202244 12_10_1_9 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNH CAGCAGRNAAATNHCTTCCCTM 16212245 12_11_1_9 CTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAG CAGCAGRNAAATAGTYWCCCTM 16222246 12_12_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNH CAGCAGGCANHCNHCTTCCCTM 16232247 12_13_1_9 CTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAG CAGCAGGCANHCAGTYWCCCTM 16242248 12_14_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNH CAGCAGGCAAATNHCYWCCCTM 16252249 12_15_1_9 CYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_2_1_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAG SWMCAGRNAAATAGTTTCCCTM 16262250 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_3_1_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAG SWMCAGGCANHCAGTTTCCCTM 16272251 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_4_1_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNH SWMCAGGCAAATNHCTTCCCTM 16282252 CTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_5_1_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAG SWMCAGGCAAATAGTYWCCCTM 16292253 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_6_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAG CAGSWMRNAAATAGTTTCCCTM 16302254 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_7_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAG CAGSWMGCANHCAGTTTCCCTM 16312255 TTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_8_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNH CAGSWMGCAAATNHCTTCCCTM 16322256 CTTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_9_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAG CAGSWMGCAAATAGTYWCCCTM 16332257 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-12_1_2_9CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAG SWMSWMGCAAATAGTTTCCCTW 16342258 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAG CAGCAGRNANHCAGTTTCCCTW 16352259 12_10_2_9 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNH CAGCAGRNAAATNHCTTCCCTW 16362260 12_11_2_9 CTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAG CAGCAGRNAAATAGTYWCCCTW 16372261 12_12_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNH CAGCAGGCANHCNHCTTCCCTW 16382262 12_13_2_9 CTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAG CAGCAGGCANHCAGTYWCCCTW 16392263 12_14_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNH CAGCAGGCAAATNHCYWCCCTW 16402264 12_15_2_9 CYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_2_2_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAG SWMCAGRNAAATAGTTTCCCTW 16412265 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_3_2_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAG SWMCAGGCANHCAGTTTCCCTW 16422266 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_4_2_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNH SWMCAGGCAAATNHCTTCCCTW 16432267 CTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_5_2_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAG SWMCAGGCAAATAGTYWCCCTW 16442268 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_6_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAG CAGSWMRNAAATAGTTTCCCTW 16452269 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_7_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAG CAGSWMGCANHCAGTTTCCCTW 16462270 TTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_8_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNH CAGSWMGCAAATNHCTTCCCTW 16472271 CTTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_9_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAG CAGSWMGCAAATAGTYWCCCTW 16482272 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-12_1_3_9CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAG SWMSWMGCAAATAGTTTCCCTC 16492273 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAG CAGCAGRNANHCAGTTTCCCTC 16502274 12_10_3_9 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNH CAGCAGRNAAATNHCTTCCCTC 16512275 12_11_3_9 CTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAG CAGCAGRNAAATAGTYWCCCTC 16522276 12_12_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNH CAGCAGGCANHCNHCTTCCCTC 16532277 12_13_3_9 CTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAG CAGCAGGCANHCAGTYWCCCTC 16542278 12_14_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNH CAGCAGGCAAATNHCYWCCCTC 16552279 12_15_3_9 CYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_2_3_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAG SWMCAGRNAAATAGTTTCCCTC 16562280 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_3_3_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAG SWMCAGGCANHCAGTTTCCCTC 16572281 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_4_3_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNH SWMCAGGCAAATNHCTTCCCTC 16582282 CTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_5_3_9CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAG SWMCAGGCAAATAGTYWCCCTC 16592283 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_6_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAG CAGSWMRNAAATAGTTTCCCTC 16602284 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_7_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAG CAGSWMGCANHCAGTTTCCCTC 16612285 TTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_8_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNH CAGSWMGCAAATNHCTTCCCTC 16622286 CTTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-12_9_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAG CAGSWMGCAAATAGTYWCCCTC 16632287 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_1_0_9CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAA SWMSWMTACGATAATCTCCCTT 16642288 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATCTCCCTT 16652289 33_10_0_9 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNH CAGCAGBHCGATNHCCTCCCTT 16662290 33_11_0_9 CCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAA CAGCAGBHCGATAATYWCCCTT 16672291 33_12_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNH CAGCAGTACNHCNHCCTCCCTT 16682292 33_13_0_9 CCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAA CAGCAGTACNHCAATYWCCCTT 16692293 33_14_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNH CAGCAGTACGATNHCYWCCCTT 16702294 33_15_0_9 CYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_2_0_9CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAA SWMCAGBHCGATAATCTCCCTT 16712295 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_3_0_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAA SWMCAGTACNHCAATCTCCCTT 16722296 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_4_0_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNH SWMCAGTACGATNHCCTCCCTT 16732297 CCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_5_0_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAA SWMCAGTACGATAATYWCCCTT 16742298 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_6_0_9CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAA CAGSWMBHCGATAATCTCCCTT 16752299 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_7_0_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAA CAGSWMTACNHCAATCTCCCTT 16762300 TCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_8_0_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNH CAGSWMTACGATNHCCTCCCTT 16772301 CCTCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_9_0_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAA CAGSWMTACGATAATYWCCCTT 16782302 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-33_1_1_9CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAA SWMSWMTACGATAATCTCCCTM 16792303 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATCTCCCTM 16802304 33_10_1_9 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNH CAGCAGBHCGATNHCCTCCCTM 16812305 33_11_1_9 CCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAA CAGCAGBHCGATAATYWCCCTM 16822306 33_12_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNH CAGCAGTACNHCNHCCTCCCTM 16832307 33_13_1_9 CCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAA CAGCAGTACNHCAATYWCCCTM 16842308 33_14_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNH CAGCAGTACGATNHCYWCCCTM 16852309 33_15_1_9 CYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_2_1_9CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAA SWMCAGBHCGATAATCTCCCTM 16862310 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_3_1_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAA SWMCAGTACNHCAATCTCCCTM 16872311 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_4_1_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNH SWMCAGTACGATNHCCTCCCTM 16882312 CCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_5_1_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAA SWMCAGTACGATAATYWCCCTM 16892313 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_6_1_9CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAA CAGSWMBHCGATAATCTCCCTM 16902314 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_7_1_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAA CAGSWMTACNHCAATCTCCCTM 16912315 TCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_8_1_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNH CAGSWMTACGATNHCCTCCCTM 16922316 CCTCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_9_1_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAA CAGSWMTACGATAATYWCCCTM 16932317 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-33_1_2_9CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAA SWMSWMTACGATAATCTCCCTW 16942318 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATCTCCCTW 16952319 33_10_2_9 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNH CAGCAGBHCGATNHCCTCCCTW 16962320 33_11_2_9 CCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAA CAGCAGBHCGATAATYWCCCTW 16972321 33_12_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNH CAGCAGTACNHCNHCCTCCCTW 16982322 33_13_2_9 CCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAA CAGCAGTACNHCAATYWCCCTW 16992323 33_14_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNH CAGCAGTACGATNHCYWCCCTW 17002324 33_15_2_9 CYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_2_2_9CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAA SWMCAGBHCGATAATCTCCCTW 17012325 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_3_2_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAA SWMCAGTACNHCAATCTCCCTW 17022326 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_4_2_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNH SWMCAGTACGATNHCCTCCCTW 17032327 CCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_5_2_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAA SWMCAGTACGATAATYWCCCTW 17042328 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_6_2_9CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAA CAGSWMBHCGATAATCTCCCTW 17052329 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_7_2_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAA CAGSWMTACNHCAATCTCCCTW 17062330 TCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_8_2_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNH CAGSWMTACGATNHCCTCCCTW 17072331 CCTCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_9_2_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAA CAGSWMTACGATAATYWCCCTW 17082332 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-33_1_3_9CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAA SWMSWMTACGATAATCTCCCTC 17092333 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATCTCCCTC 17102334 33_10_3_9 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNH CAGCAGBHCGATNHCCTCCCTC 17112335 33_11_3_9 CCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAA CAGCAGBHCGATAATYWCCCTC 17122336 33_12_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNH CAGCAGTACNHCNHCCTCCCTC 17132337 33_13_3_9 CCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAA CAGCAGTACNHCAATYWCCCTC 17142338 33_14_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNH CAGCAGTACGATNHCYWCCCTC 17152339 33_15_3_9 CYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_2_3_9CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAA SWMCAGBHCGATAATCTCCCTC 17162340 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_3_3_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAA SWMCAGTACNHCAATCTCCCTC 17172341 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_4_3_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNH SWMCAGTACGATNHCCTCCCTC 17182342 CCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_5_3_9CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAA SWMCAGTACGATAATYWCCCTC 17192343 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_6_3_9CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAA CAGSWMBHCGATAATCTCCCTC 17202344 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_7_3_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAA CAGSWMTACNHCAATCTCCCTC 17212345 TCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_8_3_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNH CAGSWMTACGATNHCCTCCCTC 17222346 CCTCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-33_9_3_9CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAA CAGSWMTACGATAATYWCCCTC 17232347 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_1_0_9CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAG SWMSWMAGCTACAGTACTCCTT 1724305 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAG CAGCAAVNABHCAGTACTCCTT 1725314 39_10_0_9 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNH CAGCAAVNATACNHCACTCCTT 1726315 39_11_0_9 CACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAG CAGCAAVNATACAGTBHCCCTT 1727316 39_12_0_9 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNH CAGCAAAGCBHCNHCACTCCTT 1728317 39_13_0_9 CACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAG CAGCAAAGCBHCAGTBHCCCTT 1729318 39_14_0_9 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNH CAGCAAAGCTACNHCBHCCCTT 1730319 39_15_0_9 CBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_2_0_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAG SWMCAAVNATACAGTACTCCTT 1731306 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_3_0_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAG SWMCAAAGCBHCAGTACTCCTT 1732307 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_4_0_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNH SWMCAAAGCTACNHCACTCCTT 1733308 CACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_5_0_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAG SWMCAAAGCTACAGTBHCCCTT 1734309 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_6_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAG CAGSWMVNATACAGTACTCCTT 1735310 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_7_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAG CAGSWMAGCBHCAGTACTCCTT 1736311 TACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_8_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNH CAGSWMAGCTACNHCACTCCTT 1737312 CACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_9_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAG CAGSWMAGCTACAGTBHCCCTT 1738313 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK1-39_1_1_9CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAG SWMSWMAGCTACAGTACTCCTM 17392348 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAG CAGCAAVNABHCAGTACTCCTM 17402349 39_10_1_9 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNH CAGCAAVNATACNHCACTCCTM 17412350 39_11_1_9 CACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAG CAGCAAVNATACAGTBHCCCTM 17422351 39_12_1_9 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNH CAGCAAAGCBHCNHCACTCCTM 17432352 39_13_1_9 CACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAG CAGCAAAGCBHCAGTBHCCCTM 17442353 39_14_1_9 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNH CAGCAAAGCTACNHCBHCCCTM 17452354 39_15_1_9 CBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_2_1_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAG SWMCAAVNATACAGTACTCCTM 17462355 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_3_1_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAG SWMCAAAGCBHCAGTACTCCTM 17472356 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_4_1_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNH SWMCAAAGCTACNHCACTCCTM 17482357 CACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_5_1_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAG SWMCAAAGCTACAGTBHCCCTM 17492358 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_6_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAG CAGSWMVNATACAGTACTCCTM 17502359 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_7_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAG CAGSWMAGCBHCAGTACTCCTM 17512360 TACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_8_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNH CAGSWMAGCTACNHCACTCCTM 17522361 CACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_9_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAG CAGSWMAGCTACAGTBHCCCTM 17532362 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK1-39_1_2_9CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAG SWMSWMAGCTACAGTACTCCTW 17542363 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAG CAGCAAVNABHCAGTACTCCTW 17552364 39_10_2_9 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNH CAGCAAVNATACNHCACTCCTW 17562365 39_11_2_9 CACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAG CAGCAAVNATACAGTBHCCCTW 17572366 39_12_2_9 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNH CAGCAAAGCBHCNHCACTCCTW 17582367 39_13_2_9 CACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAG CAGCAAAGCBHCAGTBHCCCTW 17592368 39_14_2_9 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNH CAGCAAAGCTACNHCBHCCCTW 17602369 39_15_2_9 CBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_2_2_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAG SWMCAAVNATACAGTACTCCTW 17612370 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_3_2_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAG SWMCAAAGCBHCAGTACTCCTW 17622371 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_4_2_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNH SWMCAAAGCTACNHCACTCCTW 17632372 CACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_5_2_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAG SWMCAAAGCTACAGTBHCCCTW 17642373 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_6_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAG CAGSWMVNATACAGTACTCCTW 17652374 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_7_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAG CAGSWMAGCBHCAGTACTCCTW 17662375 TACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_8_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNH CAGSWMAGCTACNHCACTCCTW 17672376 CACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_9_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAG CAGSWMAGCTACAGTBHCCCTW 17682377 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK1-39_1_3_9CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAG SWMSWMAGCTACAGTACTCCTC 17692378 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAG CAGCAAVNABHCAGTACTCCTC 17702379 39_10_3_9 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNH CAGCAAVNATACNHCACTCCTC 17712380 39_11_3_9 CACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAG CAGCAAVNATACAGTBHCCCTC 17722381 39_12_3_9 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNH CAGCAAAGCBHCNHCACTCCTC 17732382 39_13_3_9 CACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAG CAGCAAAGCBHCAGTBHCCCTC 17742383 39_14_3_9 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNH CAGCAAAGCTACNHCBHCCCTC 17752384 39_15_3_9 CBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_2_3_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAG SWMCAAVNATACAGTACTCCTC 17762385 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_3_3_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAG SWMCAAAGCBHCAGTACTCCTC 17772386 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_4_3_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNH SWMCAAAGCTACNHCACTCCTC 17782387 CACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_5_3_9CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAG SWMCAAAGCTACAGTBHCCCTC 17792388 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_6_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAG CAGSWMVNATACAGTACTCCTC 17802389 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_7_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAG CAGSWMAGCBHCAGTACTCCTC 17812390 TACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_8_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNH CAGSWMAGCTACNHCACTCCTC 17822391 CACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK1-39_9_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAG CAGSWMAGCTACAGTBHCCCTC 17832392 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_1_0_9GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCA DTSSWMGCACTCCAGACTCCTT 17842393 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACA ATGCAGVNAMNACAGACTCCTT 17852394 28_10_0_9 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSR ATGCAGVNACTCSRMACTCCTT 17862395 28_11_0_9 MACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCA ATGCAGVNACTCCAGVBCCCTT 17872396 28_12_0_9 GVBCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASR ATGCAGGCAMNASRMACTCCTT 17882397 28_13_0_9 MACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACA ATGCAGGCAMNACAGVBCCCTT 17892398 28_14_0_9 GVBCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSR ATGCAGGCACTCSRMVBCCCTT 17902399 28_15_0_9 MVBCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_2_0_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCA DTSCAGVNACTCCAGACTCCTT 17912400 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_3_0_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACA DTSCAGGCAMNACAGACTCCTT 17922401 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_4_0_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSR DTSCAGGCACTCSRMACTCCTT 17932402 MACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_5_0_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCA DTSCAGGCACTCCAGVBCCCTT 17942403 GVBCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_6_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCA ATGSWMVNACTCCAGACTCCTT 17952404 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_7_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACA ATGSWMGCAMNACAGACTCCTT 17962405 GACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_8_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSR ATGSWMGCACTCSRMACTCCTT 17972406 MACTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_9_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCA ATGSWMGCACTCCAGVBCCCTT 17982407 GVBCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK2-28_1_1_9GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCA DTSSWMGCACTCCAGACTCCTM 17992408 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACA ATGCAGVNAMNACAGACTCCTM 18002409 28_10_1_9 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSR ATGCAGVNACTCSRMACTCCTM 18012410 28_11_1_9 MACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCA ATGCAGVNACTCCAGVBCCCTM 18022411 28_12_1_9 GVBCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASR ATGCAGGCAMNASRMACTCCTM 18032412 28_13_1_9 MACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACA ATGCAGGCAMNACAGVBCCCTM 18042413 28_14_1_9 GVBCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSR ATGCAGGCACTCSRMVBCCCTM 18052414 28_15_1_9 MVBCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_2_1_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCA DTSCAGVNACTCCAGACTCCTM 18062415 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_3_1_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACA DTSCAGGCAMNACAGACTCCTM 18072416 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_4_1_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSR DTSCAGGCACTCSRMACTCCTM 18082417 MACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_5_1_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCA DTSCAGGCACTCCAGVBCCCTM 18092418 GVBCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_6_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCA ATGSWMVNACTCCAGACTCCTM 18102419 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_7_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACA ATGSWMGCAMNACAGACTCCTM 18112420 GACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_8_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSR ATGSWMGCACTCSRMACTCCTM 18122421 MACTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_9_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCA ATGSWMGCACTCCAGVBCCCTM 18132422 GVBCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK2-28_1_2_9GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCA DTSSWMGCACTCCAGACTCCTW 18142423 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACA ATGCAGVNAMNACAGACTCCTW 18152424 28_10_2_9 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSR ATGCAGVNACTCSRMACTCCTW 18162425 28_11_2_9 MACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCA ATGCAGVNACTCCAGVBCCCTW 18172426 28_12_2_9 GVBCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASR ATGCAGGCAMNASRMACTCCTW 18182427 28_13_2_9 MACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACA ATGCAGGCAMNACAGVBCCCTW 18192428 28_14_2_9 GVBCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSR ATGCAGGCACTCSRMVBCCCTW 18202429 28_15_2_9 MVBCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_2_2_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCA DTSCAGVNACTCCAGACTCCTW 18212430 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_3_2_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACA DTSCAGGCAMNACAGACTCCTW 18222431 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_4_2_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSR DTSCAGGCACTCSRMACTCCTW 18232432 MACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_5_2_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCA DTSCAGGCACTCCAGVBCCCTW 18242433 GVBCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_6_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCA ATGSWMVNACTCCAGACTCCTW 18252434 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_7_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACA ATGSWMGCAMNACAGACTCCTW 18262435 GACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_8_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSR ATGSWMGCACTCSRMACTCCTW 18272436 MACTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_9_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCA ATGSWMGCACTCCAGVBCCCTW 18282437 GVBCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK2-28_1_3_9GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCA DTSSWMGCACTCCAGACTCCTC 18292438 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACA ATGCAGVNAMNACAGACTCCTC 18302439 28_10_3_9 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSR ATGCAGVNACTCSRMACTCCTC 18312440 28_11_3_9 MACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCA ATGCAGVNACTCCAGVBCCCTC 18322441 28_12_3_9 GVBCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASR ATGCAGGCAMNASRMACTCCTC 18332442 28_13_3_9 MACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACA ATGCAGGCAMNACAGVBCCCTC 18342443 28_14_3_9 GVBCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSR ATGCAGGCACTCSRMVBCCCTC 18352444 28_15_3_9 MVBCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_2_3_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCA DTSCAGVNACTCCAGACTCCTC 18362445 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_3_3_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACA DTSCAGGCAMNACAGACTCCTC 18372446 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_4_3_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSR DTSCAGGCACTCSRMACTCCTC 18382447 MACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_5_3_9GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCA DTSCAGGCACTCCAGVBCCCTC 18392448 GVBCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_6_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCA ATGSWMVNACTCCAGACTCCTC 18402449 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_7_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACA ATGSWMGCAMNACAGACTCCTC 18412450 GACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_8_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSR ATGSWMGCACTCSRMACTCCTC 18422451 MACTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK2-28_9_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCA ATGSWMGCACTCCAGVBCCCTC 18432452 GVBCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_1_0_9CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAA SWMSWMAGAAGTAATTGGCCTT 18442453 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATTGGCCTT 18452454 11_10_0_9 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNH CAGCAGBHCAGTNHCTGGCCTT 18462455 11_11_0_9 CTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAA CAGCAGBHCAGTAATYWCCCTT 18472456 11_12_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNH CAGCAGAGANHCNHCTGGCCTT 18482457 11_13_0_9 CTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAA CAGCAGAGANHCAATYWCCCTT 18492458 11_14_0_9 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNH CAGCAGAGAAGTNHCYWCCCTT 18502459 11_15_0_9 CYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_2_0_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAA SWMCAGBHCAGTAATTGGCCTT 18512460 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_3_0_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAA SWMCAGAGANHCAATTGGCCTT 18522461 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_4_0_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNH SWMCAGAGAAGTNHCTGGCCTT 18532462 CTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_5_0_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAA SWMCAGAGAAGTAATYWCCCTT 18542463 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_6_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAA CAGSWMBHCAGTAATTGGCCTT 18552464 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_7_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAA CAGSWMAGANHCAATTGGCCTT 18562465 TTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_8_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNH CAGSWMAGAAGTNHCTGGCCTT 18572466 CTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_9_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAA CAGSWMAGAAGTAATYWCCCTT 18582467 TYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-11_1_1_9CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAA SWMSWMAGAAGTAATTGGCCTM 18592468 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATTGGCCTM 18602469 11_10_1_9 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNH CAGCAGBHCAGTNHCTGGCCTM 18612470 11_11_1_9 CTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAA CAGCAGBHCAGTAATYWCCCTM 18622471 11_12_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNH CAGCAGAGANHCNHCTGGCCTM 18632472 11_13_1_9 CTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAA CAGCAGAGANHCAATYWCCCTM 18642473 11_14_1_9 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNH CAGCAGAGAAGTNHCYWCCCTM 18652474 11_15_1_9 CYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_2_1_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAA SWMCAGBHCAGTAATTGGCCTM 18662475 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_3_1_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAA SWMCAGAGANHCAATTGGCCTM 18672476 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_4_1_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNH SWMCAGAGAAGTNHCTGGCCTM 18682477 CTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_5_1_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAA SWMCAGAGAAGTAATYWCCCTM 18692478 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_6_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAA CAGSWMBHCAGTAATTGGCCTM 18702479 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_7_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAA CAGSWMAGANHCAATTGGCCTM 18712480 TTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_8_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNH CAGSWMAGAAGTNHCTGGCCTM 18722481 CTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_9_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAA CAGSWMAGAAGTAATYWCCCTM 18732482 TYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-11_1_2_9CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAA SWMSWMAGAAGTAATTGGCCTW 18742483 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATTGGCCTW 18752484 11_10_2_9 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNH CAGCAGBHCAGTNHCTGGCCTW 18762485 11_11_2_9 CTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAA CAGCAGBHCAGTAATYWCCCTW 18772486 11_12_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNH CAGCAGAGANHCNHCTGGCCTW 18782487 11_13_2_9 CTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAA CAGCAGAGANHCAATYWCCCTW 18792488 11_14_2_9 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNH CAGCAGAGAAGTNHCYWCCCTW 18802489 11_15_2_9 CYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_2_2_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAA SWMCAGBHCAGTAATTGGCCTW 18812490 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_3_2_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAA SWMCAGAGANHCAATTGGCCTW 18822491 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_4_2_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNH SWMCAGAGAAGTNHCTGGCCTW 18832492 CTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_5_2_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAA SWMCAGAGAAGTAATYWCCCTW 18842493 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_6_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAA CAGSWMBHCAGTAATTGGCCTW 18852494 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_7_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAA CAGSWMAGANHCAATTGGCCTW 18862495 TTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_8_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNH CAGSWMAGAAGTNHCTGGCCTW 18872496 CTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_9_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAA CAGSWMAGAAGTAATYWCCCTW 18882497 TYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-11_1_3_9CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAA SWMSWMAGAAGTAATTGGCCTC 18892498 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAA CAGCAGBHCNHCAATTGGCCTC 18902499 11_10_3_9 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNH CAGCAGBHCAGTNHCTGGCCTC 18912500 11_11_3_9 CTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAA CAGCAGBHCAGTAATYWCCCTC 18922501 11_12_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNH CAGCAGAGANHCNHCTGGCCTC 18932502 11_13_3_9 CTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAA CAGCAGAGANHCAATYWCCCTC 18942503 11_14_3_9 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNH CAGCAGAGAAGTNHCYWCCCTC 18952504 11_15_3_9 CYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_2_3_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAA SWMCAGBHCAGTAATTGGCCTC 18962505 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_3_3_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAA SWMCAGAGANHCAATTGGCCTC 18972506 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_4_3_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNH SWMCAGAGAAGTNHCTGGCCTC 18982507 CTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_5_3_9CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAA SWMCAGAGAAGTAATYWCCCTC 18992508 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_6_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAA CAGSWMBHCAGTAATTGGCCTC 19002509 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_7_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAA CAGSWMAGANHCAATTGGCCTC 19012510 TTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_8_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNH CAGSWMAGAAGTNHCTGGCCTC 19022511 CTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-11_9_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAA CAGSWMAGAAGTAATYWCCCTC 19032512 TYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_1_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAA SWMSWMTACAATAATTGGCCTT 19042513 TAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNH CAGCAGBHCNHCAATTGGCCTT 19052454 15_10_0_9 CAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATNHCTGGCCTT 19062514 15_11_0_9 TNHCTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATAATYWCCCTT 19072515 15_12_0_9 TAATYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCNHCTGGCCTT 19082516 15_13_0_9 CNHCTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCAATYWCCCTT 19092293 15_14_0_9 CAATYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAA CAGCAGTACAATNHCYWCCCTT 19102517 15_15_0_9 TNHCYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_2_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAA SWMCAGBHCAATAATTGGCCTT 19112518 TAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_3_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNH SWMCAGTACNHCAATTGGCCTT 19122519 CAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_4_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATNHCTGGCCTT 19132520 TNHCTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_5_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATAATYWCCCTT 19142521 TAATYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_6_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAA CAGSWMBHCAATAATTGGCCTT 19152522 TAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_7_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNH CAGSWMTACNHCAATTGGCCTT 19162523 CAATTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_8_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATNHCTGGCCTT 19172524 TNHCTGGCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_9_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATAATYWCCCTT 19182525 TAATYWCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-15_1_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAA SWMSWMTACAATAATTGGCCTM 19192526 TAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNH CAGCAGBHCNHCAATTGGCCTM 19202469 15_10_1_9 CAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATNHCTGGCCTM 19212527 15_11_1_9 TNHCTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATAATYWCCCTM 19222528 15_12_1_9 TAATYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCNHCTGGCCTM 19232529 15_13_1_9 CNHCTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCAATYWCCCTM 19242308 15_14_1_9 CAATYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAA CAGCAGTACAATNHCYWCCCTM 19252530 15_15_1_9 TNHCYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_2_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAA SWMCAGBHCAATAATTGGCCTM 19262531 TAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_3_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNH SWMCAGTACNHCAATTGGCCTM 19272532 CAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_4_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATNHCTGGCCTM 19282533 TNHCTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_5_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATAATYWCCCTM 19292534 TAATYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_6_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAA CAGSWMBHCAATAATTGGCCTM 19302535 TAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_7_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNH CAGSWMTACNHCAATTGGCCTM 19312536 CAATTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_8_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATNHCTGGCCTM 19322537 TNHCTGGCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_9_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATAATYWCCCTM 19332538 TAATYWCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-15_1_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAA SWMSWMTACAATAATTGGCCTW 19342539 TAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNH CAGCAGBHCNHCAATTGGCCTW 19352484 15_10_2_9 CAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATNHCTGGCCTW 19362540 15_11_2_9 TNHCTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATAATYWCCCTW 19372541 15_12_2_9 TAATYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCNHCTGGCCTW 19382542 15_13_2_9 CNHCTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCAATYWCCCTW 19392323 15_14_2_9 CAATYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAA CAGCAGTACAATNHCYWCCCTW 19402543 15_15_2_9 TNHCYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_2_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAA SWMCAGBHCAATAATTGGCCTW 19412544 TAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_3_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNH SWMCAGTACNHCAATTGGCCTW 19422545 CAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_4_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATNHCTGGCCTW 19432546 TNHCTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_5_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATAATYWCCCTW 19442547 TAATYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_6_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAA CAGSWMBHCAATAATTGGCCTW 19452548 TAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_7_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNH CAGSWMTACNHCAATTGGCCTW 19462549 CAATTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_8_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATNHCTGGCCTW 19472550 TNHCTGGCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_9_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATAATYWCCCTW 19482551 TAATYWCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-15_1_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAA SWMSWMTACAATAATTGGCCTC 19492552 TAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNH CAGCAGBHCNHCAATTGGCCTC 19502499 15_10_3_9 CAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATNHCTGGCCTC 19512553 15_11_3_9 TNHCTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAA CAGCAGBHCAATAATYWCCCTC 19522554 15_12_3_9 TAATYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCNHCTGGCCTC 19532555 15_13_3_9 CNHCTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNH CAGCAGTACNHCAATYWCCCTC 19542338 15_14_3_9 CAATYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAA CAGCAGTACAATNHCYWCCCTC 19552556 15_15_3_9 TNHCYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_2_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAA SWMCAGBHCAATAATTGGCCTC 19562557 TAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_3_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNH SWMCAGTACNHCAATTGGCCTC 19572558 CAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_4_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATNHCTGGCCTC 19582559 TNHCTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_5_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAA SWMCAGTACAATAATYWCCCTC 19592560 TAATYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_6_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAA CAGSWMBHCAATAATTGGCCTC 19602561 TAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_7_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNH CAGSWMTACNHCAATTGGCCTC 19612562 CAATTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_8_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATNHCTGGCCTC 19622563 TNHCTGGCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-15_9_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAA CAGSWMTACAATAATYWCCCTC 19632564 TAATYWCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_1_0_9CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAG SWMSWMTACGGAAGTAGTCCTT 19642565 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAG CAGCAGBHCBHCAGTAGTCCTT 19652566 20_10_0_9 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVN CAGCAGBHCGGAVNCAGTCCTT 19662567 20_11_0_9 CAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAG CAGCAGBHCGGAAGTBHCCCTT 19672568 20_12_0_9 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVN CAGCAGTACBHCVNCAGTCCTT 19682569 20_13_0_9 CAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAG CAGCAGTACBHCAGTBHCCCTT 19692570 20_14_0_9 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVN CAGCAGTACGGAVNCBHCCCTT 19702571 20_15_0_9 CBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_2_0_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAG SWMCAGBHCGGAAGTAGTCCTT 19712572 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_3_0_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAG SWMCAGTACBHCAGTAGTCCTT 19722573 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_4_0_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVN SWMCAGTACGGAVNCAGTCCTT 19732574 CAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_5_0_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAG SWMCAGTACGGAAGTBHCCCTT 19742575 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_6_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAG CAGSWMBHCGGAAGTAGTCCTT 19752576 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_7_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAG CAGSWMTACBHCAGTAGTCCTT 19762577 TAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_8_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVN CAGSWMTACGGAVNCAGTCCTT 19772578 CAGTCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_9_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAG CAGSWMTACGGAAGTBHCCCTT 19782579 TBHCCCTTWCACTTTTGGCGGAGGGACCAAG WCACT VK3-20_1_1_9CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAG SWMSWMTACGGAAGTAGTCCTM 19792580 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAG CAGCAGBHCBHCAGTAGTCCTM 19802581 20_10_1_9 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVN CAGCAGBHCGGAVNCAGTCCTM 19812582 20_11_1_9 CAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAG CAGCAGBHCGGAAGTBHCCCTM 19822583 20_12_1_9 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVN CAGCAGTACBHCVNCAGTCCTM 19832584 20_13_1_9 CAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAG CAGCAGTACBHCAGTBHCCCTM 19842585 20_14_1_9 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVN CAGCAGTACGGAVNCBHCCCTM 19852586 20_15_1_9 CBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_2_1_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAG SWMCAGBHCGGAAGTAGTCCTM 19862587 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_3_1_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAG SWMCAGTACBHCAGTAGTCCTM 19872588 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_4_1_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVN SWMCAGTACGGAVNCAGTCCTM 19882589 CAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_5_1_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAG SWMCAGTACGGAAGTBHCCCTM 19892590 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_6_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAG CAGSWMBHCGGAAGTAGTCCTM 19902591 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_7_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAG CAGSWMTACBHCAGTAGTCCTM 19912592 TAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_8_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVN CAGSWMTACGGAVNCAGTCCTM 19922593 CAGTCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_9_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAG CAGSWMTACGGAAGTBHCCCTM 19932594 TBHCCCTMTCACTTTTGGCGGAGGGACCAAG TCACT VK3-20_1_2_9CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAG SWMSWMTACGGAAGTAGTCCTW 19942595 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAG CAGCAGBHCBHCAGTAGTCCTW 19952596 20_10_2_9 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVN CAGCAGBHCGGAVNCAGTCCTW 19962597 20_11_2_9 CAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAG CAGCAGBHCGGAAGTBHCCCTW 19972598 20_12_2_9 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVN CAGCAGTACBHCVNCAGTCCTW 19982599 20_13_2_9 CAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAG CAGCAGTACBHCAGTBHCCCTW 19992600 20_14_2_9 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVN CAGCAGTACGGAVNCBHCCCTW 20002601 20_15_2_9 CBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_2_2_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAG SWMCAGBHCGGAAGTAGTCCTW 20012602 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_3_2_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAG SWMCAGTACBHCAGTAGTCCTW 20022603 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_4_2_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVN SWMCAGTACGGAVNCAGTCCTW 20032604 CAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_5_2_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAG SWMCAGTACGGAAGTBHCCCTW 20042605 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_6_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAG CAGSWMBHCGGAAGTAGTCCTW 20052606 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_7_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAG CAGSWMTACBHCAGTAGTCCTW 20062607 TAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_8_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVN CAGSWMTACGGAVNCAGTCCTW 20072608 CAGTCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_9_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAG CAGSWMTACGGAAGTBHCCCTW 20082609 TBHCCCTWGGACTTTTGGCGGAGGGACCAAG GGACT VK3-20_1_3_9CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAG SWMSWMTACGGAAGTAGTCCTC 20092610 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAG CAGCAGBHCBHCAGTAGTCCTC 20102611 20_10_3_9 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVN CAGCAGBHCGGAVNCAGTCCTC 20112612 20_11_3_9 CAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAG CAGCAGBHCGGAAGTBHCCCTC 20122613 20_12_3_9 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVN CAGCAGTACBHCVNCAGTCCTC 20132614 20_13_3_9 CAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAG CAGCAGTACBHCAGTBHCCCTC 20142615 20_14_3_9 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVN CAGCAGTACGGAVNCBHCCCTC 20152616 20_15_3_9 CBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_2_3_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAG SWMCAGBHCGGAAGTAGTCCTC 20162617 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_3_3_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAG SWMCAGTACBHCAGTAGTCCTC 20172618 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_4_3_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVN SWMCAGTACGGAVNCAGTCCTC 20182619 CAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_5_3_9CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAG SWMCAGTACGGAAGTBHCCCTC 20192620 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_6_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAG CAGSWMBHCGGAAGTAGTCCTC 20202621 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_7_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAG CAGSWMTACBHCAGTAGTCCTC 20212622 TAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_8_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVN CAGSWMTACGGAVNCAGTCCTC 20222623 CAGTCCTCCTACTTTTGGCGGAGGGACCAAG CTACT VK3-20_9_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAG CAGSWMTACGGAAGTBHCCCTC 20232624 TBHCCCTCCTACTTTTGGCGGAGGGACCAAG CTACT Jumping Trimer VK1-05_t1_0_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTAC CAGCAGBHCVRMMBCTACYCTTWC2024 2625 YCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t1_1_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTAC CAGCAGBHCVRMMBCTACYCTMTC2025 2626 YCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t1_2_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTAC CAGCAGBHCVRMMBCTACYCTWGG2026 2627 YCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-05_t1_3_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTAC CAGCAGBHCVRMMBCTACYCTCCT2027 2628 YCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-05_t2_0_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWC CAGCAGBHCAATMBCYWCYCTTWC2028 2629 YCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t2_1_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWC CAGCAGBHCAATMBCYWCYCTMTC2029 2630 YCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t2_2_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWC CAGCAGBHCAATMBCYWCYCTWGG2030 2631 YCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-05_t2_3_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWC CAGCAGBHCAATMBCYWCYCTCCT2031 2632 YCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-05_t3_0_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWC CAGCAGBHCVRMAGTYWCYCTTWC2032 2633 YCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t3_1_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWC CAGCAGBHCVRMAGTYWCYCTMTC2033 2634 YCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t3_2_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWC CAGCAGBHCVRMAGTYWCYCTWGG2034 2635 YCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-05_t3_3_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWC CAGCAGBHCVRMAGTYWCYCTCCT2035 2636 YCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-05_t4_0_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWC CAGCAGTACVRMMBCYWCYCTTWC2036 2637 YCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t4_1_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWC CAGCAGTACVRMMBCYWCYCTMTC2037 2638 YCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-05_t4_2_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWC CAGCAGTACVRMMBCYWCYCTWGG2038 2639 YCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-05_t4_3_9CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWC CAGCAGTACVRMMBCYWCYCTCCT2039 2640 YCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-12_t1_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTC CAGCAGRNANHCNHCTTCCCTTWC2040 2641 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t1_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTC CAGCAGRNANHCNHCTTCCCTMTC2041 2642 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t1_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTC CAGCAGRNANHCNHCTTCCCTWGG2042 2643 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-12_t1_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTC CAGCAGRNANHCNHCTTCCCTCCT2043 2644 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-12_t2_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWC CAGCAGRNANHCAGTYWCCCTTWC2044 2645 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t2_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWC CAGCAGRNANHCAGTYWCCCTMTC2045 2646 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t2_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWC CAGCAGRNANHCAGTYWCCCTWGG2046 2647 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-12_t2_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWC CAGCAGRNANHCAGTYWCCCTCCT2047 2648 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-12_t3_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWC CAGCAGRNAAATNHCYWCCCTTWC2048 2649 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t3_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWC CAGCAGRNAAATNHCYWCCCTMTC2049 2650 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t3_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWC CAGCAGRNAAATNHCYWCCCTWGG2050 2651 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-12_t3_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWC CAGCAGRNAAATNHCYWCCCTCCT2051 2652 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-12_t4_0_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWC CAGCAGGCANHCNHCYWCCCTTWC2052 2653 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t4_1_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWC CAGCAGGCANHCNHCYWCCCTMTC2053 2654 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-12_t4_2_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWC CAGCAGGCANHCNHCYWCCCTWGG2054 2655 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-12_t4_3_9CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWC CAGCAGGCANHCNHCYWCCCTCCT2055 2656 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-33_t1_0_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTC CAGCAGBHCNHCNHCCTCCCTTWC2056 2657 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t1_1_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTC CAGCAGBHCNHCNHCCTCCCTMTC2057 2658 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t1_2_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTC CAGCAGBHCNHCNHCCTCCCTWGG2058 2659 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-33_t1_3_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTC CAGCAGBHCNHCNHCCTCCCTCCT2059 2660 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-33_t2_0_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTTWC2060 2661 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t2_1_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTMTC2061 2662 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t2_2_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTWGG2062 2663 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-33_t2_3_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTCCT2063 2664 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-33_t3_0_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWC CAGCAGBHCGATNHCYWCCCTTWC2064 2665 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t3_1_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWC CAGCAGBHCGATNHCYWCCCTMTC2065 2666 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t3_2_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWC CAGCAGBHCGATNHCYWCCCTWGG2066 2667 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-33_t3_3_9CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWC CAGCAGBHCGATNHCYWCCCTCCT2067 2668 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-33_t4_0_9CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWC CAGCAGTACNHCNHCYWCCCTTWC2068 2669 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t4_1_9CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWC CAGCAGTACNHCNHCYWCCCTMTC2069 2670 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-33_t4_2_9CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWC CAGCAGTACNHCNHCYWCCCTWGG2070 2671 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-33_t4_3_9CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWC CAGCAGTACNHCNHCYWCCCTCCT2071 2672 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-39_t1_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACT CAGCAAVNABHCNHCACTCCTTWC2072 320 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t1_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACT CAGCAAVNABHCNHCACTCCTMTC2073 2673 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t1_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACT CAGCAAVNABHCNHCACTCCTWGG2074 2674 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-39_t1_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACT CAGCAAVNABHCNHCACTCCTCCT2075 2675 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-39_t2_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHC CAGCAAVNABHCAGTBHCCCTTWC2076 321 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t2_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHC CAGCAAVNABHCAGTBHCCCTMTC2077 2676 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t2_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHC CAGCAAVNABHCAGTBHCCCTWGG2078 2677 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-39_t2_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHC CAGCAAVNABHCAGTBHCCCTCCT2079 2678 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-39_t3_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHC CAGCAAVNATACNHCBHCCCTTWC2080 322 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t3_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHC CAGCAAVNATACNHCBHCCCTMTC2081 2679 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t3_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHC CAGCAAVNATACNHCBHCCCTWGG2082 2680 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-39_t3_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHC CAGCAAVNATACNHCBHCCCTCCT2083 2681 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK1-39_t4_0_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHC CAGCAAAGCBHCNHCBHCCCTTWC2084 323 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t4_1_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHC CAGCAAAGCBHCNHCBHCCCTMTC2085 2682 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK1-39_t4_2_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHC CAGCAAAGCBHCNHCBHCCCTWGG2086 2683 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK1-39_t4_3_9CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHC CAGCAAAGCBHCNHCBHCCCTCCT2087 2684 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK2-28_t1_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACT ATGCAGVNAMNASRMACTCCTTWC2088 2685 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t1_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACT ATGCAGVNAMNASRMACTCCTMTC2089 2686 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t1_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACT ATGCAGVNAMNASRMACTCCTWGG2090 2687 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK2-28_t1_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACT ATGCAGVNAMNASRMACTCCTCCT2091 2688 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK2-28_t2_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBC ATGCAGVNAMNACAGVBCCCTTWC2092 2689 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t2_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBC ATGCAGVNAMNACAGVBCCCTMTC2093 2690 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t2_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBC ATGCAGVNAMNACAGVBCCCTWGG2094 2691 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK2-28_t2_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBC ATGCAGVNAMNACAGVBCCCTCCT2095 2692 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK2-28_t3_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBC ATGCAGVNACTCSRMVBCCCTTWC2096 2693 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t3_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBC ATGCAGVNACTCSRMVBCCCTMTC2097 2694 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t3_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBC ATGCAGVNACTCSRMVBCCCTWGG2098 2695 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK2-28_t3_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBC ATGCAGVNACTCSRMVBCCCTCCT2099 2696 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK2-28_t4_0_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBC ATGCAGGCAMNASRMVBCCCTTWC2100 2697 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t4_1_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBC ATGCAGGCAMNASRMVBCCCTMTC2101 2698 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK2-28_t4_2_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBC ATGCAGGCAMNASRMVBCCCTWGG2102 2699 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK2-28_t4_3_9GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBC ATGCAGGCAMNASRMVBCCCTCCT2103 2700 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-11_t1_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGG CAGCAGBHCNHCNHCTGGCCTTWC2104 2701 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t1_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGG CAGCAGBHCNHCNHCTGGCCTMTC2105 2702 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t1_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGG CAGCAGBHCNHCNHCTGGCCTWGG2106 2703 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-11_t1_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGG CAGCAGBHCNHCNHCTGGCCTCCT2107 2704 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-11_t2_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTTWC2108 2661 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t2_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTMTC2109 2662 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t2_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTWGG2110 2663 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-11_t2_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWC CAGCAGBHCNHCAATYWCCCTCCT2111 2664 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-11_t3_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWC CAGCAGBHCAGTNHCYWCCCTTWC2112 2705 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t3_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWC CAGCAGBHCAGTNHCYWCCCTMTC2113 2706 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t3_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWC CAGCAGBHCAGTNHCYWCCCTWGG2114 2707 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-11_t3_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWC CAGCAGBHCAGTNHCYWCCCTCCT2115 2708 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-11_t4_0_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWC CAGCAGAGANHCNHCYWCCCTTWC2116 2709 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t4_1_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWC CAGCAGAGANHCNHCYWCCCTMTC2117 2710 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-11_t4_2_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWC CAGCAGAGANHCNHCYWCCCTWGG2118 2711 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-11_t4_3_9CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWC CAGCAGAGANHCNHCYWCCCTCCT2119 2712 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-15_t1_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHC CAGCAGBHCNHCNHCTGGCCTTWC2120 2701 TGGCCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t1_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHC CAGCAGBHCNHCNHCTGGCCTMTC2121 2702 TGGCCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t1_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHC CAGCAGBHCNHCNHCTGGCCTWGG2122 2703 TGGCCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-15_t1_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHC CAGCAGBHCNHCNHCTGGCCTCCT2123 2704 TGGCCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-15_t2_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAAT CAGCAGBHCNHCAATYWCCCTTWC2124 2661 YWCCCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t2_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAAT CAGCAGBHCNHCAATYWCCCTMTC2125 2662 YWCCCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t2_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAAT CAGCAGBHCNHCAATYWCCCTWGG2126 2663 YWCCCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-15_t2_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAAT CAGCAGBHCNHCAATYWCCCTCCT2127 2664 YWCCCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-15_t3_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHC CAGCAGBHCAATNHCYWCCCTTWC2128 2713 YWCCCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t3_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHC CAGCAGBHCAATNHCYWCCCTMTC2129 2714 YWCCCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t3_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHC CAGCAGBHCAATNHCYWCCCTWGG2130 2715 YWCCCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-15_t3_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHC CAGCAGBHCAATNHCYWCCCTCCT2131 2716 YWCCCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-15_t4_0_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHC CAGCAGTACNHCNHCYWCCCTTWC2132 2669 YWCCCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t4_1_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHC CAGCAGTACNHCNHCYWCCCTMTC2133 2670 YWCCCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-15_t4_2_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHC CAGCAGTACNHCNHCYWCCCTWGG2134 2671 YWCCCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-15_t4_3_9CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHC CAGCAGTACNHCNHCYWCCCTCCT2135 2672 YWCCCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-20_t1_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGT CAGCAGBHCBHCVNCAGTCCTTWC2136 2717 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t1_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGT CAGCAGBHCBHCVNCAGTCCTMTC2137 2718 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t1_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGT CAGCAGBHCBHCVNCAGTCCTWGG2138 2719 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-20_t1_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGT CAGCAGBHCBHCVNCAGTCCTCCT2139 2720 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-20_t2_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTTWC2140 2721 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t2_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTMTC2141 2722 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t2_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTWGG2142 2723 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-20_t2_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTCCT2143 2724 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-20_t3_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHC CAGCAGBHCGGAVNCBHCCCTTWC2144 2725 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t3_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHC CAGCAGBHCGGAVNCBHCCCTMTC2145 2726 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t3_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHC CAGCAGBHCGGAVNCBHCCCTWGG2146 2727 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-20_t3_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHC CAGCAGBHCGGAVNCBHCCCTCCT2147 2728 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK3-20_t4_0_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHC CAGCAGTACBHCVNCBHCCCTTWC2148 2729 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t4_1_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHC CAGCAGTACBHCVNCBHCCCTMTC2149 2730 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK3-20_t4_2_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHC CAGCAGTACBHCVNCBHCCCTWGG2150 2731 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK3-20_t4_3_9CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHC CAGCAGTACBHCVNCBHCCCTCCT2151 2732 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK4-01_t1_0_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACT CAGCAGBHCBHCNHCACTCCTTWC2152 2733 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t1_1_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACT CAGCAGBHCBHCNHCACTCCTMTC2153 2734 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t1_2_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACT CAGCAGBHCBHCNHCACTCCTWGG2154 2735 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK4-01_t1_3_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACT CAGCAGBHCBHCNHCACTCCTCCT2155 2736 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK4-01_t2_0_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTTWC2156 2721 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t2_1_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTMTC2157 2722 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t2_2_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTWGG2158 2723 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK4-01_t2_3_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHC CAGCAGBHCBHCAGTBHCCCTCCT2159 2724 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK4-01_t3_0_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHC CAGCAGBHCTACNHCBHCCCTTWC2160 2737 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t3_1_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHC CAGCAGBHCTACNHCBHCCCTMTC2161 2738 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t3_2_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHC CAGCAGBHCTACNHCBHCCCTWGG2162 2739 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK4-01_t3_3_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHC CAGCAGBHCTACNHCBHCCCTCCT2163 2740 CCTCCTACTTTTGGCGGAGGGACCAAG ACT VK4-01_t4_0_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHC CAGCAGTACBHCNHCBHCCCTTWC2164 2741 CCTTWCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t4_1_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHC CAGCAGTACBHCNHCBHCCCTMTC2165 2742 CCTMTCACTTTTGGCGGAGGGACCAAG ACT VK4-01_t4_2_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHC CAGCAGTACBHCNHCBHCCCTWGG2166 2743 CCTWGGACTTTTGGCGGAGGGACCAAG ACT VK4-01_t4_3_9GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHC CAGCAGTACBHCNHCBHCCCTCCT2167 2744 CCTCCTACTTTTGGCGGAGGGACCAAG ACT

TABLE 7 Oligonucleotide sequences for exemplary VK jumping dimer andtrimer sequences with CDRL3 length 10. Portion of SEQ ID OligonucleotideSEQ ID NO Corresponding to NO (CDRL3 Name Sequence of SynthesizedOligonucleotide CDRL3 Proper (Oligo) Portion) Jumping DimerVK1-05_1_0_10 CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAGSWMSWMTACAATAGTTACYC 2745 3213 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_10_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGCAGCAGBHCVRMAGTTACYC 2746 3214 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_11_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCAGCAGBHCAATMBCTACYC 2747 3215 CTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_12_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAGCAGCAGBHCAATAGTYWCYC 2748 3216 TYWCYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_13_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCAGCAGTACVRMMBCTACYC 2749 3217 CTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_14_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAGCAGCAGTACVRMAGTYWCYC 2750 3218 TYWCYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_15_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMBCAGCAGTACAATMBCYWCYC 2751 3219 CYWCYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_2_0_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAGSWMCAGBHCAATAGTTACYC 2752 3220 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_3_0_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAGSWMCAGTACVRMAGTTACYC 2753 3221 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_4_0_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMBSWMCAGTACAATMBCTACYC 2754 3222 CTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_5_0_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAGSWMCAGTACAATAGTYWCYC 2755 3223 TYWCYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_6_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAGCAGSWMBHCAATAGTTACYC 2756 3224 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_7_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAGCAGSWMTACVRMAGTTACYC 2757 3225 TTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_8_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMBCAGSWMTACAATMBCTACYC 2758 3226 CTACYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_9_0_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAGCAGSWMTACAATAGTYWCYC 2759 3227 TYWCYCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-05_1_1_10 CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAGSWMSWMTACAATAGTTACYC 2760 3228 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_10_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGCAGCAGBHCVRMAGTTACYC 2761 3229 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_11_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCAGCAGBHCAATMBCTACYC 2762 3230 CTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_12_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAGCAGCAGBHCAATAGTYWCYC 2763 3231 TYWCYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_13_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCAGCAGTACVRMMBCTACYC 2764 3232 CTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_14_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAGCAGCAGTACVRMAGTYWCYC 2765 3233 TYWCYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_15_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMBCAGCAGTACAATMBCYWCYC 2766 3234 CYWCYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_2_1_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAGSWMCAGBHCAATAGTTACYC 2767 3235 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_3_1_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAGSWMCAGTACVRMAGTTACYC 2768 3236 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_4_1_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMBSWMCAGTACAATMBCTACYC 2769 3237 CTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_5_1_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAGSWMCAGTACAATAGTYWCYC 2770 3238 TYWCYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_6_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAGCAGSWMBHCAATAGTTACYC 2771 3239 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_7_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAGCAGSWMTACVRMAGTTACYC 2772 3240 TTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_8_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMBCAGSWMTACAATMBCTACYC 2773 3241 CTACYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_9_1_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAGCAGSWMTACAATAGTYWCYC 2774 3242 TYWCYCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-05_1_2_10 CCTGATGATTTTGCAACTTATTACTGCSWMSWMTACAATAGSWMSWMTACAATAGTTACYC 2775 3243 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_10_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGCAGCAGBHCVRMAGTTACYC 2776 3244 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_11_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCAGCAGBHCAATMBCTACYC 2777 3245 CTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_12_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATAGCAGCAGBHCAATAGTYWCYC 2778 3246 TYWCYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_13_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCAGCAGTACVRMMBCTACYC 2779 3247 CTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_14_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMAGCAGCAGTACVRMAGTYWCYC 2780 3248 TYWCYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_15_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACAATMBCAGCAGTACAATMBCYWCYC 2781 3249 CYWCYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_2_2_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGBHCAATAGSWMCAGBHCAATAGTTACYC 2782 3250 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_3_2_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACVRMAGSWMCAGTACVRMAGTTACYC 2783 3251 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_4_2_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATMBSWMCAGTACAATMBCTACYC 2784 3252 CTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_5_2_10 CCTGATGATTTTGCAACTTATTACTGCSWMCAGTACAATAGSWMCAGTACAATAGTYWCYC 2785 3253 TYWCYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_6_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMBHCAATAGCAGSWMBHCAATAGTTACYC 2786 3254 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_7_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACVRMAGCAGSWMTACVRMAGTTACYC 2787 3255 TTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_8_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATMBCAGSWMTACAATMBCTACYC 2788 3256 CTACYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-05_9_2_10 CCTGATGATTTTGCAACTTATTACTGCCAGSWMTACAATAGCAGSWMTACAATAGTYWCYC 2789 3257 TYWCYCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_1_0_10 CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAGSWMSWMGCAAATAGTTTCCC 2790 3258 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_10_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGCAGCAGRNANHCAGTTTCCC 2791 3259 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_11_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCAGCAGRNAAATNHCTTCCC 2792 3260 CTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_12_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAGCAGCAGRNAAATAGTYWCCC 2793 3261 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_13_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCAGCAGGCANHCNHCTTCCC 2794 3262 CTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_14_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAGCAGCAGGCANHCAGTYWCCC 2795 3263 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_15_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNHCAGCAGGCAAATNHCYWCCC 2796 3264 CYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_2_0_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAGSWMCAGRNAAATAGTTTCCC 2797 3265 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_3_0_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAGSWMCAGGCANHCAGTTTCCC 2798 3266 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_4_0_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNHSWMCAGGCAAATNHCTTCCC 2799 3267 CTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_5_0_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAGSWMCAGGCAAATAGTYWCCC 2800 3268 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_6_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAGCAGSWMRNAAATAGTTTCCC 2801 3269 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_7_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAGCAGSWMGCANHCAGTTTCCC 2802 3270 TTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_8_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNHCAGSWMGCAAATNHCTTCCC 2803 3271 CTTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_9_0_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAGCAGSWMGCAAATAGTYWCCC 2804 3272 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-12_1_1_10 CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAGSWMSWMGCAAATAGTTTCCC 2805 3273 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_10_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGCAGCAGRNANHCAGTTTCCC 2806 3274 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_11_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCAGCAGRNAAATNHCTTCCC 2807 3275 CTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_12_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAGCAGCAGRNAAATAGTYWCCC 2808 3276 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_13_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCAGCAGGCANHCNHCTTCCC 2809 3277 CTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_14_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAGCAGCAGGCANHCAGTYWCCC 2810 3278 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_15_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNHCAGCAGGCAAATNHCYWCCC 2811 3279 CYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_2_1_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAGSWMCAGRNAAATAGTTTCCC 2812 3280 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_3_1_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAGSWMCAGGCANHCAGTTTCCC 2813 3281 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_4_1_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNHSWMCAGGCAAATNHCTTCCC 2814 3282 CTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_5_1_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAGSWMCAGGCAAATAGTYWCCC 2815 3283 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_6_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAGCAGSWMRNAAATAGTTTCCC 2816 3284 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_7_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAGCAGSWMGCANHCAGTTTCCC 2817 3285 TTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_8_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNHCAGSWMGCAAATNHCTTCCC 2818 3286 CTTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_9_1_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAGCAGSWMGCAAATAGTYWCCC 2819 3287 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-12_1_2_10 CCTGAAGATTTTGCAACTTATTACTGTSWMSWMGCAAATAGSWMSWMGCAAATAGTTTCCC 2820 3288 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_10_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGCAGCAGRNANHCAGTTTCCC 2821 3289 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_11_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCAGCAGRNAAATNHCTTCCC 2822 3290 CTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_12_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATAGCAGCAGRNAAATAGTYWCCC 2823 3291 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_13_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCAGCAGGCANHCNHCTTCCC 2824 3292 CTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_14_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCAGCAGCAGGCANHCAGTYWCCC 2825 3293 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_15_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCAAATNHCAGCAGGCAAATNHCYWCCC 2826 3294 CYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_2_2_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGRNAAATAGSWMCAGRNAAATAGTTTCCC 2827 3295 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_3_2_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCANHCAGSWMCAGGCANHCAGTTTCCC 2828 3296 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_4_2_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATNHSWMCAGGCAAATNHCTTCCC 2829 3297 CTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_5_2_10 CCTGAAGATTTTGCAACTTATTACTGTSWMCAGGCAAATAGSWMCAGGCAAATAGTYWCCC 2830 3298 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_6_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMRNAAATAGCAGSWMRNAAATAGTTTCCC 2831 3299 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_7_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCANHCAGCAGSWMGCANHCAGTTTCCC 2832 3300 TTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_8_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATNHCAGSWMGCAAATNHCTTCCC 2833 3301 CTTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-12_9_2_10 CCTGAAGATTTTGCAACTTATTACTGTCAGSWMGCAAATAGCAGSWMGCAAATAGTYWCCC 2834 3302 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_1_0_10 CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAASWMSWMTACGATAATCTCCC 2835 3303 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_10_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATCTCCC 2836 3304 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_11_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCAGCAGBHCGATNHCCTCCC 2837 3305 CCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_12_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAACAGCAGBHCGATAATYWCCC 2838 3306 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_13_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCAGCAGTACNHCNHCCTCCC 2839 3307 CCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_14_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAACAGCAGTACNHCAATYWCCC 2840 3308 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_15_0_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNHCAGCAGTACGATNHCYWCCC 2841 3309 CYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_2_0_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAASWMCAGBHCGATAATCTCCC 2842 3310 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_3_0_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAASWMCAGTACNHCAATCTCCC 2843 3311 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_4_0_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNHSWMCAGTACGATNHCCTCCC 2844 3312 CCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_5_0_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAASWMCAGTACGATAATYWCCC 2845 3313 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_6_0_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAACAGSWMBHCGATAATCTCCC 2846 3314 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_7_0_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAACAGSWMTACNHCAATCTCCC 2847 3315 TCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_8_0_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNHCAGSWMTACGATNHCCTCCC 2848 3316 CCTCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_9_0_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAACAGSWMTACGATAATYWCCC 2849 3317 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-33_1_1_10 CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAASWMSWMTACGATAATCTCCC 2850 3318 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_10_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATCTCCC 2851 3319 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_11_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCAGCAGBHCGATNHCCTCCC 2852 3320 CCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_12_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAACAGCAGBHCGATAATYWCCC 2853 3321 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_13_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCAGCAGTACNHCNHCCTCCC 2854 3322 CCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_14_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAACAGCAGTACNHCAATYWCCC 2855 3323 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_15_1_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNHCAGCAGTACGATNHCYWCCC 2856 3324 CYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_2_1_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAASWMCAGBHCGATAATCTCCC 2857 3325 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_3_1_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAASWMCAGTACNHCAATCTCCC 2858 3326 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_4_1_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNHSWMCAGTACGATNHCCTCCC 2859 3327 CCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_5_1_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAASWMCAGTACGATAATYWCCC 2860 3328 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_6_1_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAACAGSWMBHCGATAATCTCCC 2861 3329 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_7_1_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAACAGSWMTACNHCAATCTCCC 2862 3330 TCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_8_1_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNHCAGSWMTACGATNHCCTCCC 2863 3331 CCTCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_9_1_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAACAGSWMTACGATAATYWCCC 2864 3332 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-33_1_2_10 CCTGAAGATATTGCAACATATTACTGTSWMSWMTACGATAASWMSWMTACGATAATCTCCC 2865 3333 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_10_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATCTCCC 2866 3334 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_11_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCAGCAGBHCGATNHCCTCCC 2867 3335 CCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_12_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATAACAGCAGBHCGATAATYWCCC 2868 3336 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_13_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCAGCAGTACNHCNHCCTCCC 2869 3337 CCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_14_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCAACAGCAGTACNHCAATYWCCC 2870 3338 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_15_2_10 CCTGAAGATATTGCAACATATTACTGTCAGCAGTACGATNHCAGCAGTACGATNHCYWCCC 2871 3339 CYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_2_2_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGBHCGATAASWMCAGBHCGATAATCTCCC 2872 3340 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_3_2_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACNHCAASWMCAGTACNHCAATCTCCC 2873 3341 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_4_2_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATNHSWMCAGTACGATNHCCTCCC 2874 3342 CCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_5_2_10 CCTGAAGATATTGCAACATATTACTGTSWMCAGTACGATAASWMCAGTACGATAATYWCCC 2875 3343 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_6_2_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMBHCGATAACAGSWMBHCGATAATCTCCC 2876 3344 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_7_2_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACNHCAACAGSWMTACNHCAATCTCCC 2877 3345 TCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_8_2_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATNHCAGSWMTACGATNHCCTCCC 2878 3346 CCTCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-33_9_2_10 CCTGAAGATATTGCAACATATTACTGTCAGSWMTACGATAACAGSWMTACGATAATYWCCC 2879 3347 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_1_0_10 CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAGSWMSWMAGCTACAGTACTCC 2880 3348 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_10_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGCAGCAAVNABHCAGTACTCC 2881 3349 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_11_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCAGCAAVNATACNHCACTCC 2882 3350 CACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_12_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAGCAGCAAVNATACAGTBHCCC 2883 3351 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_13_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCAGCAAAGCBHCNHCACTCC 2884 3352 CACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_14_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAGCAGCAAAGCBHCAGTBHCCC 2885 3353 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_15_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNHCAGCAAAGCTACNHCBHCCC 2886 3354 CBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_2_0_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAGSWMCAAVNATACAGTACTCC 2887 3355 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_3_0_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAGSWMCAAAGCBHCAGTACTCC 2888 3356 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_4_0_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNHSWMCAAAGCTACNHCACTCC 2889 3357 CACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_5_0_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAGSWMCAAAGCTACAGTBHCCC 2890 3358 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_6_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAGCAGSWMVNATACAGTACTCC 2891 3359 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_7_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAGCAGSWMAGCBHCAGTACTCC 2892 3360 TACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_8_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNHCAGSWMAGCTACNHCACTCC 2893 3361 CACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_9_0_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAGCAGSWMAGCTACAGTBHCCC 2894 3362 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK1-39_1_1_10 CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAGSWMSWMAGCTACAGTACTCC 2895 3363 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_10_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGCAGCAAVNABHCAGTACTCC 2896 3364 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_11_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCAGCAAVNATACNHCACTCC 2897 3365 CACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_12_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAGCAGCAAVNATACAGTBHCCC 2898 3366 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_13_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCAGCAAAGCBHCNHCACTCC 2899 3367 CACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_14_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAGCAGCAAAGCBHCAGTBHCCC 2900 3368 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_15_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNHCAGCAAAGCTACNHCBHCCC 2901 3369 CBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_2_1_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAGSWMCAAVNATACAGTACTCC 2902 3370 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_3_1_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAGSWMCAAAGCBHCAGTACTCC 2903 3371 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_4_1_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNHSWMCAAAGCTACNHCACTCC 2904 3372 CACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_5_1_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAGSWMCAAAGCTACAGTBHCCC 2905 3373 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_6_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAGCAGSWMVNATACAGTACTCC 2906 3374 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_7_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAGCAGSWMAGCBHCAGTACTCC 2907 3375 TACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_8_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNHCAGSWMAGCTACNHCACTCC 2908 3376 CACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_9_1_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAGCAGSWMAGCTACAGTBHCCC 2909 3377 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK1-39_1_2_10 CCTGAAGATTTTGCAACTTACTACTGTSWMSWMAGCTACAGSWMSWMAGCTACAGTACTCC 2910 3378 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_10_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGCAGCAAVNABHCAGTACTCC 2911 3379 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_11_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCAGCAAVNATACNHCACTCC 2912 3380 CACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_12_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACAGCAGCAAVNATACAGTBHCCC 2913 3381 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_13_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCAGCAAAGCBHCNHCACTCC 2914 3382 CACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_14_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCAGCAGCAAAGCBHCAGTBHCCC 2915 3383 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_15_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCTACNHCAGCAAAGCTACNHCBHCCC 2916 3384 CBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_2_2_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAVNATACAGSWMCAAVNATACAGTACTCC 2917 3385 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_3_2_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCBHCAGSWMCAAAGCBHCAGTACTCC 2918 3386 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_4_2_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACNHSWMCAAAGCTACNHCACTCC 2919 3387 CACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_5_2_10 CCTGAAGATTTTGCAACTTACTACTGTSWMCAAAGCTACAGSWMCAAAGCTACAGTBHCCC 2920 3388 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_6_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMVNATACAGCAGSWMVNATACAGTACTCC 2921 3389 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_7_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCBHCAGCAGSWMAGCBHCAGTACTCC 2922 3390 TACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_8_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACNHCAGSWMAGCTACNHCACTCC 2923 3391 CACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK1-39_9_2_10 CCTGAAGATTTTGCAACTTACTACTGTCAGSWMAGCTACAGCAGSWMAGCTACAGTBHCCC 2924 3392 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_1_0_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCADTSSWMGCACTCCAGACTCC 2925 3393 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_10_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAATGCAGVNAMNACAGACTCC 2926 3394 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_11_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRATGCAGVNACTCSRMACTCC 2927 3395 MACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_12_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCAATGCAGVNACTCCAGVBCCC 2928 3396 GVBCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_13_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRATGCAGGCAMNASRMACTCC 2929 3397 MACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_14_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACAATGCAGGCAMNACAGVBCCC 2930 3398 GVBCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_15_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSRATGCAGGCACTCSRMVBCCC 2931 3399 MVBCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_2_0_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCADTSCAGVNACTCCAGACTCC 2932 3400 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_3_0_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACADTSCAGGCAMNACAGACTCC 2933 3401 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_4_0_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSRDTSCAGGCACTCSRMACTCC 2934 3402 MACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_5_0_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCADTSCAGGCACTCCAGVBCCC 2935 3403 GVBCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_6_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCAATGSWMVNACTCCAGACTCC 2936 3404 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_7_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACAATGSWMGCAMNACAGACTCC 2937 3405 GACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_8_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSRATGSWMGCACTCSRMACTCC 2938 3406 MACTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_9_0_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCAATGSWMGCACTCCAGVBCCC 2939 3407 GVBCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK2-28_1_1_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCADTSSWMGCACTCCAGACTCC 2940 3408 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_10_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAATGCAGVNAMNACAGACTCC 2941 3409 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_11_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRATGCAGVNACTCSRMACTCC 2942 3410 MACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_12_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCAATGCAGVNACTCCAGVBCCC 2943 3411 GVBCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_13_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRATGCAGGCAMNASRMACTCC 2944 3412 MACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_14_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACAATGCAGGCAMNACAGVBCCC 2945 3413 GVBCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_15_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSRATGCAGGCACTCSRMVBCCC 2946 3414 MVBCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_2_1_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCADTSCAGVNACTCCAGACTCC 2947 3415 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_3_1_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACADTSCAGGCAMNACAGACTCC 2948 3416 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_4_1_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSRDTSCAGGCACTCSRMACTCC 2949 3417 MACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_5_1_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCADTSCAGGCACTCCAGVBCCC 2950 3418 GVBCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_6_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCAATGSWMVNACTCCAGACTCC 2951 3419 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_7_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACAATGSWMGCAMNACAGACTCC 2952 3420 GACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_8_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSRATGSWMGCACTCSRMACTCC 2953 3421 MACTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_9_1_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCAATGSWMGCACTCCAGVBCCC 2954 3422 GVBCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK2-28_1_2_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSSWMGCACTCCADTSSWMGCACTCCAGACTCC 2955 3423 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_10_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAATGCAGVNAMNACAGACTCC 2956 3424 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_11_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRATGCAGVNACTCSRMACTCC 2957 3425 MACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_12_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCCAATGCAGVNACTCCAGVBCCC 2958 3426 GVBCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_13_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRATGCAGGCAMNASRMACTCC 2959 3427 MACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_14_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNACAATGCAGGCAMNACAGVBCCC 2960 3428 GVBCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_15_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCACTCSRATGCAGGCACTCSRMVBCCC 2961 3429 MVBCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_2_2_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGVNACTCCADTSCAGVNACTCCAGACTCC 2962 3430 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_3_2_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCAMNACADTSCAGGCAMNACAGACTCC 2963 3431 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_4_2_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCSRDTSCAGGCACTCSRMACTCC 2964 3432 MACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_5_2_10 GCTGAGGATGTTGGGGTTTATTACTGCDTSCAGGCACTCCADTSCAGGCACTCCAGVBCCC 2965 3433 GVBCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_6_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMVNACTCCAATGSWMVNACTCCAGACTCC 2966 3434 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_7_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCAMNACAATGSWMGCAMNACAGACTCC 2967 3435 GACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_8_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCSRATGSWMGCACTCSRMACTCC 2968 3436 MACTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK2-28_9_2_10 GCTGAGGATGTTGGGGTTTATTACTGCATGSWMGCACTCCAATGSWMGCACTCCAGVBCCC 2969 3437 GVBCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_1_0_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAASWMSWMAGAAGTAATTGGCC 2970 3438 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_10_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATTGGCC 2971 3439 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_11_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCAGCAGBHCAGTNHCTGGCC 2972 3440 CTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_12_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAACAGCAGBHCAGTAATYWCCC 2973 3441 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_13_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCAGCAGAGANHCNHCTGGCC 2974 3442 CTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_14_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAACAGCAGAGANHCAATYWCCC 2975 3443 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_15_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNHCAGCAGAGAAGTNHCYWCCC 2976 3444 CYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_2_0_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAASWMCAGBHCAGTAATTGGCC 2977 3445 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_3_0_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAASWMCAGAGANHCAATTGGCC 2978 3446 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_4_0_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNHSWMCAGAGAAGTNHCTGGCC 2979 3447 CTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_5_0_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAASWMCAGAGAAGTAATYWCCC 2980 3448 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_6_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAACAGSWMBHCAGTAATTGGCC 2981 3449 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_7_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAACAGSWMAGANHCAATTGGCC 2982 3450 TTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_8_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNHCAGSWMAGAAGTNHCTGGCC 2983 3451 CTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_9_0_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAACAGSWMAGAAGTAATYWCCC 2984 3452 TYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-11_1_1_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAASWMSWMAGAAGTAATTGGCC 2985 3453 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_10_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATTGGCC 2986 3454 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_11_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCAGCAGBHCAGTNHCTGGCC 2987 3455 CTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_12_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAACAGCAGBHCAGTAATYWCCC 2988 3456 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_13_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCAGCAGAGANHCNHCTGGCC 2989 3457 CTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_14_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAACAGCAGAGANHCAATYWCCC 2990 3458 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_15_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNHCAGCAGAGAAGTNHCYWCCC 2991 3459 CYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_2_1_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAASWMCAGBHCAGTAATTGGCC 2992 3460 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_3_1_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAASWMCAGAGANHCAATTGGCC 2993 3461 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_4_1_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNHSWMCAGAGAAGTNHCTGGCC 2994 3462 CTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_5_1_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAASWMCAGAGAAGTAATYWCCC 2995 3463 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_6_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAACAGSWMBHCAGTAATTGGCC 2996 3464 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_7_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAACAGSWMAGANHCAATTGGCC 2997 3465 TTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_8_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNHCAGSWMAGAAGTNHCTGGCC 2998 3466 CTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_9_1_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAACAGSWMAGAAGTAATYWCCC 2999 3467 TYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-11_1_2_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMSWMAGAAGTAASWMSWMAGAAGTAATTGGCC 3000 3468 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_10_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAACAGCAGBHCNHCAATTGGCC 3001 3469 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_11_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCAGCAGBHCAGTNHCTGGCC 3002 3470 CTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_12_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTAACAGCAGBHCAGTAATYWCCC 3003 3471 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_13_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCAGCAGAGANHCNHCTGGCC 3004 3472 CTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_14_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCAACAGCAGAGANHCAATYWCCC 3005 3473 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_15_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGAAGTNHCAGCAGAGAAGTNHCYWCCC 3006 3474 CYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_2_2_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAGTAASWMCAGBHCAGTAATTGGCC 3007 3475 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_3_2_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGANHCAASWMCAGAGANHCAATTGGCC 3008 3476 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_4_2_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTNHSWMCAGAGAAGTNHCTGGCC 3009 3477 CTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_5_2_10 CCTGAAGATTTTGCAGTTTATTACTGTSWMCAGAGAAGTAASWMCAGAGAAGTAATYWCCC 3010 3478 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_6_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAGTAACAGSWMBHCAGTAATTGGCC 3011 3479 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_7_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGANHCAACAGSWMAGANHCAATTGGCC 3012 3480 TTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_8_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTNHCAGSWMAGAAGTNHCTGGCC 3013 3481 CTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-11_9_2_10 CCTGAAGATTTTGCAGTTTATTACTGTCAGSWMAGAAGTAACAGSWMAGAAGTAATYWCCC 3014 3482 TYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_1_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAASWMSWMTACAATAATTGGCC 3015 3483 TAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_10_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAGCAGBHCNHCAATTGGCC 3016 3439 CAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_11_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATNHCTGGCC 3017 3484 TNHCTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_12_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATAATYWCCC 3018 3485 TAATYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_13_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCNHCTGGCC 3019 3486 CNHCTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_14_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCAATYWCCC 3020 3308 CAATYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_15_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAACAGCAGTACAATNHCYWCCC 3021 3487 TNHCYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_2_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAASWMCAGBHCAATAATTGGCC 3022 3488 TAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_3_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNHSWMCAGTACNHCAATTGGCC 3023 3489 CAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_4_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATNHCTGGCC 3024 3490 TNHCTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_5_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATAATYWCCC 3025 3491 TAATYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_6_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAACAGSWMBHCAATAATTGGCC 3026 3492 TAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_7_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNHCAGSWMTACNHCAATTGGCC 3027 3493 CAATTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_8_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATNHCTGGCC 3028 3494 TNHCTGGCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_9_0_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATAATYWCCC 3029 3495 TAATYWCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-15_1_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAASWMSWMTACAATAATTGGCC 3030 3496 TAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_10_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAGCAGBHCNHCAATTGGCC 3031 3454 CAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_11_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATNHCTGGCC 3032 3497 TNHCTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_12_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATAATYWCCC 3033 3498 TAATYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_13_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCNHCTGGCC 3034 3499 CNHCTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_14_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCAATYWCCC 3035 3323 CAATYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_15_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAACAGCAGTACAATNHCYWCCC 3036 3500 TNHCYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_2_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAASWMCAGBHCAATAATTGGCC 3037 3501 TAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_3_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNHSWMCAGTACNHCAATTGGCC 3038 3502 CAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_4_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATNHCTGGCC 3039 3503 TNHCTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_5_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATAATYWCCC 3040 3504 TAATYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_6_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAACAGSWMBHCAATAATTGGCC 3041 3505 TAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_7_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNHCAGSWMTACNHCAATTGGCC 3042 3506 CAATTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_8_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATNHCTGGCC 3043 3507 TNHCTGGCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_9_1_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATAATYWCCC 3044 3508 TAATYWCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-15_1_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMSWMTACAASWMSWMTACAATAATTGGCC 3045 3509 TAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_10_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAGCAGBHCNHCAATTGGCC 3046 3469 CAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_11_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATNHCTGGCC 3047 3510 TNHCTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_12_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAACAGCAGBHCAATAATYWCCC 3048 3511 TAATYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_13_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCNHCTGGCC 3049 3512 CNHCTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_14_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCAGCAGTACNHCAATYWCCC 3050 3338 CAATYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_15_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACAACAGCAGTACAATNHCYWCCC 3051 3513 TNHCYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_2_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGBHCAASWMCAGBHCAATAATTGGCC 3052 3514 TAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_3_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACNHSWMCAGTACNHCAATTGGCC 3053 3515 CAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_4_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATNHCTGGCC 3054 3516 TNHCTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_5_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTSWMCAGTACAASWMCAGTACAATAATYWCCC 3055 3517 TAATYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_6_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMBHCAACAGSWMBHCAATAATTGGCC 3056 3518 TAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_7_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACNHCAGSWMTACNHCAATTGGCC 3057 3519 CAATTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_8_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATNHCTGGCC 3058 3520 TNHCTGGCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-15_9_2_10 CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGSWMTACAACAGSWMTACAATAATYWCCC 3059 3521 TAATYWCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_1_0_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAGSWMSWMTACGGAAGTAGTCC 3060 3522 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_10_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGCAGCAGBHCBHCAGTAGTCC 3061 3523 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_11_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCAGCAGBHCGGAVNCAGTCC 3062 3524 CAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_12_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAGCAGCAGBHCGGAAGTBHCCC 3063 3525 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_13_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCAGCAGTACBHCVNCAGTCC 3064 3526 CAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_14_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAGCAGCAGTACBHCAGTBHCCC 3065 3527 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_15_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVNCAGCAGTACGGAVNCBHCCC 3066 3528 CBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_2_0_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAGSWMCAGBHCGGAAGTAGTCC 3067 3529 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_3_0_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAGSWMCAGTACBHCAGTAGTCC 3068 3530 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_4_0_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVNSWMCAGTACGGAVNCAGTCC 3069 3531 CAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_5_0_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAGSWMCAGTACGGAAGTBHCCC 3070 3532 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_6_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAGCAGSWMBHCGGAAGTAGTCC 3071 3533 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_7_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAGCAGSWMTACBHCAGTAGTCC 3072 3534 TAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_8_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVNCAGSWMTACGGAVNCAGTCC 3073 3535 CAGTCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_9_0_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAGCAGSWMTACGGAAGTBHCCC 3074 3536 TBHCCCTCCTTWCACTTTTGGCGGAGGGACCAAGTCCTTWCACT VK3-20_1_1_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAGSWMSWMTACGGAAGTAGTCC 3075 3537 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_10_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGCAGCAGBHCBHCAGTAGTCC 3076 3538 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_11_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCAGCAGBHCGGAVNCAGTCC 3077 3539 CAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_12_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAGCAGCAGBHCGGAAGTBHCCC 3078 3540 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_13_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCAGCAGTACBHCVNCAGTCC 3079 3541 CAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_14_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAGCAGCAGTACBHCAGTBHCCC 3080 3542 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_15_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVNCAGCAGTACGGAVNCBHCCC 3081 3543 CBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_2_1_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAGSWMCAGBHCGGAAGTAGTCC 3082 3544 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_3_1_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAGSWMCAGTACBHCAGTAGTCC 3083 3545 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_4_1_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVNSWMCAGTACGGAVNCAGTCC 3084 3546 CAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_5_1_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAGSWMCAGTACGGAAGTBHCCC 3085 3547 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_6_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAGCAGSWMBHCGGAAGTAGTCC 3086 3548 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_7_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAGCAGSWMTACBHCAGTAGTCC 3087 3549 TAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_8_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVNCAGSWMTACGGAVNCAGTCC 3088 3550 CAGTCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_9_1_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAGCAGSWMTACGGAAGTBHCCC 3089 3551 TBHCCCTCCTMTCACTTTTGGCGGAGGGACCAAGTCCTMTCACT VK3-20_1_2_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMSWMTACGGAAGSWMSWMTACGGAAGTAGTCC 3090 3552 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_10_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGCAGCAGBHCBHCAGTAGTCC 3091 3553 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_11_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCAGCAGBHCGGAVNCAGTCC 3092 3554 CAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_12_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAAGCAGCAGBHCGGAAGTBHCCC 3093 3555 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_13_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCAGCAGTACBHCVNCAGTCC 3094 3556 CAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_14_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCAGCAGCAGTACBHCAGTBHCCC 3095 3557 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_15_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGAVNCAGCAGTACGGAVNCBHCCC 3096 3558 CBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_2_2_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGBHCGGAAGSWMCAGBHCGGAAGTAGTCC 3097 3559 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_3_2_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACBHCAGSWMCAGTACBHCAGTAGTCC 3098 3560 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_4_2_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAVNSWMCAGTACGGAVNCAGTCC 3099 3561 CAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_5_2_10 CCTGAAGATTTTGCAGTGTATTACTGTSWMCAGTACGGAAGSWMCAGTACGGAAGTBHCCC 3100 3562 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_6_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMBHCGGAAGCAGSWMBHCGGAAGTAGTCC 3101 3563 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_7_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACBHCAGCAGSWMTACBHCAGTAGTCC 3102 3564 TAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_8_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAVNCAGSWMTACGGAVNCAGTCC 3103 3565 CAGTCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT VK3-20_9_2_10 CCTGAAGATTTTGCAGTGTATTACTGTCAGSWMTACGGAAGCAGSWMTACGGAAGTBHCCC 3104 3566 TBHCCCTCCTWGGACTTTTGGCGGAGGGACCAAGTCCTWGGACT Jumping Trimer VK1-05_t1_0_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTACY CAGCAGBHCVRMMBCTACYCTCC3105 3567 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-05_t1_1_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTACY CAGCAGBHCVRMMBCTACYCTCC3106 3568 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-05_t1_2_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMMBCTACY CAGCAGBHCVRMMBCTACYCTCC3107 3569 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-05_t2_0_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWCY CAGCAGBHCAATMBCYWCYCTCC3108 3570 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-05_t2_1_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWCY CAGCAGBHCAATMBCYWCYCTCC3109 3571 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-05_t2_2_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCAATMBCYWCY CAGCAGBHCAATMBCYWCYCTCC3110 3572 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-05_t3_0_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWCY CAGCAGBHCVRMAGTYWCYCTCC3111 3573 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-05_t3_1_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWCY CAGCAGBHCVRMAGTYWCYCTCC3112 3574 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-05_t3_2_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGBHCVRMAGTYWCY CAGCAGBHCVRMAGTYWCYCTCC3113 3575 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-05_t4_0_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWCY CAGCAGTACVRMMBCYWCYCTCC3114 3576 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-05_t4_1_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWCY CAGCAGTACVRMMBCYWCYCTCC3115 3577 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-05_t4_2_10CCTGATGATTTTGCAACTTATTACTGCCAGCAGTACVRMMBCYWCY CAGCAGTACVRMMBCYWCYCTCC3116 3578 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-12_t1_0_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTCC CAGCAGRNANHCNHCTTCCCTCC3117 3579 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-12_t1_1_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTCC CAGCAGRNANHCNHCTTCCCTCC3118 3580 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-12_t1_2_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCNHCTTCC CAGCAGRNANHCNHCTTCCCTCC3119 3581 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-12_t2_0_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWCC CAGCAGRNANHCAGTYWCCCTCC3120 3582 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-12_t2_1_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWCC CAGCAGRNANHCAGTYWCCCTCC3121 3583 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-12_t2_2_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNANHCAGTYWCC CAGCAGRNANHCAGTYWCCCTCC3122 3584 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-12_t3_0_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWCC CAGCAGRNAAATNHCYWCCCTCC3123 3585 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-12_t3_1_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWCC CAGCAGRNAAATNHCYWCCCTCC3124 3586 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-12_t3_2_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGRNAAATNHCYWCC CAGCAGRNAAATNHCYWCCCTCC3125 3587 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-12_t4_0_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWCC CAGCAGGCANHCNHCYWCCCTCC3126 3588 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-12_t4_1_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWCC CAGCAGGCANHCNHCYWCCCTCC3127 3589 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-12_t4_2_10CCTGAAGATTTTGCAACTTATTACTGTCAGCAGGCANHCNHCYWCC CAGCAGGCANHCNHCYWCCCTCC3128 3590 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-33_t1_0_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTCC CAGCAGBHCNHCNHCCTCCCTCC3129 3591 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-33_t1_1_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTCC CAGCAGBHCNHCNHCCTCCCTCC3130 3592 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-33_t1_2_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCNHCCTCC CAGCAGBHCNHCNHCCTCCCTCC3131 3593 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-33_t2_0_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3132 3594 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-33_t2_1_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3133 3595 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-33_t2_2_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3134 3596 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-33_t3_0_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWCC CAGCAGBHCGATNHCYWCCCTCC3135 3597 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-33_t3_1_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWCC CAGCAGBHCGATNHCYWCCCTCC3136 3598 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-33_t3_2_10CCTGAAGATATTGCAACATATTACTGTCAGCAGBHCGATNHCYWCC CAGCAGBHCGATNHCYWCCCTCC3137 3599 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-33_t4_0_10CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWCC CAGCAGTACNHCNHCYWCCCTCC3138 3600 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-33_t4_1_10CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWCC CAGCAGTACNHCNHCYWCCCTCC3139 3601 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-33_t4_2_10CCTGAAGATATTGCAACATATTACTGTCAGCAGTACNHCNHCYWCC CAGCAGTACNHCNHCYWCCCTCC3140 3602 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-39_t1_0_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACTC CAGCAAVNABHCNHCACTCCTCC3141 3603 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-39_t1_1_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACTC CAGCAAVNABHCNHCACTCCTCC3142 3604 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-39_t1_2_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCNHCACTC CAGCAAVNABHCNHCACTCCTCC3143 3605 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-39_t2_0_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHCC CAGCAAVNABHCAGTBHCCCTCC3144 3606 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-39_t2_1_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHCC CAGCAAVNABHCAGTBHCCCTCC3145 3607 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-39_t2_2_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNABHCAGTBHCC CAGCAAVNABHCAGTBHCCCTCC3146 3608 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-39_t3_0_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHCC CAGCAAVNATACNHCBHCCCTCC3147 3609 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-39_t3_1_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHCC CAGCAAVNATACNHCBHCCCTCC3148 3610 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-39_t3_2_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAVNATACNHCBHCC CAGCAAVNATACNHCBHCCCTCC3149 3611 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK1-39_t4_0_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHCC CAGCAAAGCBHCNHCBHCCCTCC3150 3612 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK1-39_t4_1_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHCC CAGCAAAGCBHCNHCBHCCCTCC3151 3613 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK1-39_t4_2_10CCTGAAGATTTTGCAACTTACTACTGTCAGCAAAGCBHCNHCBHCC CAGCAAAGCBHCNHCBHCCCTCC3152 3614 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK2-28_t1_0_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACTC ATGCAGVNAMNASRMACTCCTCC3153 3615 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK2-28_t1_1_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACTC ATGCAGVNAMNASRMACTCCTCC3154 3616 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK2-28_t1_2_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNASRMACTC ATGCAGVNAMNASRMACTCCTCC3155 3617 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK2-28_t2_0_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBCC ATGCAGVNAMNACAGVBCCCTCC3156 3618 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK2-28_t2_1_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBCC ATGCAGVNAMNACAGVBCCCTCC3157 3619 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK2-28_t2_2_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNAMNACAGVBCC ATGCAGVNAMNACAGVBCCCTCC3158 3620 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK2-28_t3_0_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBCC ATGCAGVNACTCSRMVBCCCTCC3159 3621 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK2-28_t3_1_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBCC ATGCAGVNACTCSRMVBCCCTCC3160 3622 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK2-28_t3_2_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGVNACTCSRMVBCC ATGCAGVNACTCSRMVBCCCTCC3161 3623 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK2-28_t4_0_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBCC ATGCAGGCAMNASRMVBCCCTCC3162 3624 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK2-28_t4_1_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBCC ATGCAGGCAMNASRMVBCCCTCC3163 3625 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK2-28_t4_2_10GCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCAMNASRMVBCC ATGCAGGCAMNASRMVBCCCTCC3164 3626 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-11_t1_0_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGGC CAGCAGBHCNHCNHCTGGCCTCC3165 3627 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-11_t1_1_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGGC CAGCAGBHCNHCNHCTGGCCTCC3166 3628 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-11_t1_2_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCTGGC CAGCAGBHCNHCNHCTGGCCTCC3167 3629 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-11_t2_0_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3168 3594 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-11_t2_1_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3169 3595 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-11_t2_2_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATYWCC CAGCAGBHCNHCAATYWCCCTCC3170 3596 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-11_t3_0_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWCC CAGCAGBHCAGTNHCYWCCCTCC3171 3630 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-11_t3_1_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWCC CAGCAGBHCAGTNHCYWCCCTCC3172 3631 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-11_t3_2_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAGTNHCYWCC CAGCAGBHCAGTNHCYWCCCTCC3173 3632 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-11_t4_0_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWCC CAGCAGAGANHCNHCYWCCCTCC3174 3633 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-11_t4_1_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWCC CAGCAGAGANHCNHCYWCCCTCC3175 3634 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-11_t4_2_10CCTGAAGATTTTGCAGTTTATTACTGTCAGCAGAGANHCNHCYWCC CAGCAGAGANHCNHCYWCCCTCC3176 3635 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-15_t1_0_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCT CAGCAGBHCNHCNHCTGGCCTCC3177 3627 GGCCTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-15_t1_1_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCT CAGCAGBHCNHCNHCTGGCCTCC3178 3628 GGCCTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-15_t1_2_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCNHCT CAGCAGBHCNHCNHCTGGCCTCC3179 3629 GGCCTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-15_t2_0_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATY CAGCAGBHCNHCAATYWCCCTCC3180 3594 WCCCTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-15_t2_1_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATY CAGCAGBHCNHCAATYWCCCTCC3181 3595 WCCCTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-15_t2_2_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCNHCAATY CAGCAGBHCNHCAATYWCCCTCC3182 3596 WCCCTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-15_t3_0_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHCY CAGCAGBHCAATNHCYWCCCTCC3183 3636 WCCCTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-15_t3_1_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHCY CAGCAGBHCAATNHCYWCCCTCC3184 3637 WCCCTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-15_t3_2_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGBHCAATNHCY CAGCAGBHCAATNHCYWCCCTCC3185 3638 WCCCTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-15_t4_0_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHCY CAGCAGTACNHCNHCYWCCCTCC3186 3600 WCCCTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-15_t4_1_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHCY CAGCAGTACNHCNHCYWCCCTCC3187 3601 WCCCTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-15_t4_2_10CAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTACNHCNHCY CAGCAGTACNHCNHCYWCCCTCC3188 3602 WCCCTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-20_t1_0_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGTC CAGCAGBHCBHCVNCAGTCCTCC3189 3639 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-20_t1_1_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGTC CAGCAGBHCBHCVNCAGTCCTCC3190 3640 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-20_t1_2_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCVNCAGTC CAGCAGBHCBHCVNCAGTCCTCC3191 3641 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-20_t2_0_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3192 3642 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-20_t2_1_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3193 3643 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-20_t2_2_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3194 3644 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-20_t3_0_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHCC CAGCAGBHCGGAVNCBHCCCTCC3195 3645 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-20_t3_1_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHCC CAGCAGBHCGGAVNCBHCCCTCC3196 3646 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-20_t3_2_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGBHCGGAVNCBHCC CAGCAGBHCGGAVNCBHCCCTCC3197 3647 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK3-20_t4_0_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHCC CAGCAGTACBHCVNCBHCCCTCC3198 3648 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK3-20_t4_1_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHCC CAGCAGTACBHCVNCBHCCCTCC3199 3649 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK3-20_t4_2_10CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACBHCVNCBHCC CAGCAGTACBHCVNCBHCCCTCC3200 3650 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK4-01_t1_0_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACTC CAGCAGBHCBHCNHCACTCCTCC3201 3651 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK4-01_t1_1_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACTC CAGCAGBHCBHCNHCACTCCTCC3202 3652 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK4-01_t1_2_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCNHCACTC CAGCAGBHCBHCNHCACTCCTCC3203 3653 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK4-01_t2_0_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3204 3642 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK4-01_t2_1_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3205 3643 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK4-01_t2_2_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCBHCAGTBHCC CAGCAGBHCBHCAGTBHCCCTCC3206 3644 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK4-01_t3_0_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHCC CAGCAGBHCTACNHCBHCCCTCC3207 3654 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK4-01_t3_1_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHCC CAGCAGBHCTACNHCBHCCCTCC3208 3655 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK4-01_t3_2_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGBHCTACNHCBHCC CAGCAGBHCTACNHCBHCCCTCC3209 3656 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT VK4-01_t4_0_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHCC CAGCAGTACBHCNHCBHCCCTCC3210 3657 CTCCTTWCACTTTTGGCGGAGGGACCAAG TTWCACT VK4-01_t4_1_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHCC CAGCAGTACBHCNHCBHCCCTCC3211 3658 CTCCTMTCACTTTTGGCGGAGGGACCAAG TMTCACT VK4-01_t4_2_10GCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTACBHCNHCBHCC CAGCAGTACBHCNHCBHCCCTCC3212 3659 CTCCTWGGACTTTTGGCGGAGGGACCAAG TWGGACT

TABLE 8 Number of unique CDRL3 amino acid sequences in exemplary jumpingdimer (“JD”) and jumping trimer (“JT”) VK libraries and comparison toVK-v1.0. L = 8 aa L = 9 aa L = 10 aa Jumping Dimer Germline JD VK-v1.0JD VK-v1.0 JD VK-v1.0 VK1-05 3549 3072 7098 6144 6084 13824 VK1-12 52502016 5250 2016 4500 5184 VK1-33 5502 4032 5502 4032 4716 10368 VK1-397224 3024 7224 3024 6192 7776 VK2-28 4396 2016 4396 2016 3768 5184VK3-11 6048 2352 6048 2016 5184 6048 VK3-15 5789 2016 5789 2352 49625184 VK3-20 6405 2016 6671 2016 5490 5184 VK4-01 NPE* 2016 6405 2592NPE* 5184 Total 4.42 * 10⁴ 2.26 * 10⁴ 5.44 * 10⁴ 2.62 * 10⁴ 4.09 * 10⁴6.39 * 10⁴ Jumping Trimer Germline JT VK-v1.0 JT VK-v1.0 JT VK-v1.0VK1-05 7872 3072 13776 6144 11808 13824 VK1-12 14469 2016 14469 201612402 5184 VK1-33 15960 4032 15960 4032 13680 10368 VK1-39 28980 302428980 3024 24840 7776 VK2-28 12306 2016 12306 2016 10548 5184 VK3-1118900 2352 18900 2016 16200 6048 VK3-15 18256 2016 18256 2352 15648 5184VK3-20 23688 2016 23688 2016 20304 5184 VK4-01 23688 2016 23688 259220304 5184 Total 1.64 * 10⁵ 2.26 * 10⁴ 1.70 * 10⁵ 2.62 * 10⁴ 1.46 * 10⁵6.39 * 10⁴ *Not Presently Exemplified. However, given the teachings ofthe specification, a person of ordinary skill in the art could readilyproduce a library of such lengths, and these lengths are included withinthe scope of the invention.

TABLE 9Matching output for exemplary CDRH3 sequences from the HPS and TSP1.Amino acid mismatches in the theoretical design are indicated in bold.Test CDRH3 Sequence SEQ SEQ SEQ SEQ Case from HPS ID NO Mismatches TN1ID NO DH ID NO N2 H3-JH ID NO 1 RTAHHFDY 3660 0 R TA H HFDY 4582 2.1VGIVGAASY 3661 0 V GIVGA 3751 AS Y 2.2 VGIVGAASY 3661 0 VG IVGA 3755 ASY 3.1 DRYSGHDLGY 3662 1 DR YSGYD 4389 LG Y 4.1 GIAAADSNWLDP 3663 1 —GIAAA 4448 D SNWFDP 4600 4.2 GIAAADSNWLDP 3663 1 — IAAA 4452 D SNWFDP4600 5.1 ERTINWGWGGVYAFDI 3664 3 EGTG 3707 NWG GGV YAFDI 4540 5.2ERTINWGWGGVYAFDI 3663 3 EGTG 3707 NWG WGT YAFDI 4540 5.3ERTINWGWGGVYAFDI 3664 3 ERGG 3719 NWG GGV YAFDI 4540 5.4ERTINWGWGGVYAFDI 3664 3 ERGG 3719 NWG WGT YAFDI 4540

TABLE 10 Theoretical segment pool of 212 TN1 sequencescontained in Theoretical Segment Pool 1 (TSP1). TN1 Amino TN1 AminoSegment Acid Segment Acid SEQ Name Sequence Name Sequence ID NO P000 —P107 PT n/a P001 E P108 EPT n/a P002 D P109 DPT n/a P003 G P110 GPT n/aP004 EG P111 PV n/a P005 DG P112 EPV n/a P006 GG P113 DPV n/a P007 RP114 GPV n/a P008 ER P115 RP n/a P009 DR P116 ERP n/a P010 GR P117 DRPn/a P011 S P118 GRP n/a P012 ES P119 SP n/a P013 DS P120 ESP n/a P014 GSP121 DSP n/a P015 P P122 GSP n/a P016 EP P123 LP n/a P017 DP P124 ELPn/a P018 GP P125 DLP n/a P019 L P126 GLP n/a P020 EL P127 AP n/a P021 DLP128 EAP n/a P022 GL P129 DAP n/a P023 A P130 GAP n/a P024 EA P131 TPn/a P025 DA P132 ETP n/a P026 GA P133 DTP n/a P027 T P134 GTP n/a P028ET P135 VP n/a P029 DT P136 EVP n/a P030 GT P137 DVP n/a P031 V P138 GVPn/a P032 EV P139 AGG n/a P033 DV P140 EAGG 3665 P034 GV P141 DAGG 3666P035 EGG P142 GAGG 3667 P036 DGG P143 EGAG 3668 P037 GGG P144 DGAG 3669P038 EGR P145 GGAG 3670 P039 DGR P146 EGGA 3671 P040 GGR P147 DGGA 3672P041 EGS P148 GGGA 3673 P042 DGS P149 EGGG 3674 P043 GGS P150 DGGG 3675P044 EGP P151 GGGG 3676 P045 DGP P152 EGGL 3677 P046 GGP P153 DGGL 3678P047 EGL P154 GGGL 3679 P048 DGL P155 EGGP 3680 P049 GGL P156 DGGP 3681P050 EGA P157 GGGP 3682 P051 DGA P158 EGGR 3683 P052 GGA P159 DGGR 3684P053 EGT P160 GGGR 3685 P054 DGT P161 EGGS 3686 P055 GGT P162 DGGS 3687P056 EGV P163 GGGS 3688 P057 DGV P164 EGGT 3689 P058 GGV P165 DGGT 3690P059 RG P166 GGGT 3691 P060 ERG P167 EGGV 3692 P061 DRG P168 DGGV 3693P062 GRG P169 GGGV 3694 P063 SG P170 EGLG 3695 P064 ESG P171 DGLG 3696P065 DSG P172 GGLG 3697 P066 GSG P173 EGPG 3698 P067 PG P174 DGPG 3699P068 EPG P175 GGPG 3700 P069 DPG P176 EGRG 3701 P070 GPG P177 DGRG 3702P071 LG P178 GGRG 3703 P072 ELG P179 EGSG 3704 P073 DLG P180 DGSG 3705P074 GLG P181 GGSG 3706 P075 AG P182 EGTG 3707 P076 EAG P183 DGTG 3708P077 DAG P184 GGTG 3709 P078 GAG P185 EGVG 3710 P079 TG P186 DGVG 3711P080 ETG P187 GGVG 3712 P081 DTG P188 LGG n/a P082 GTG P189 ELGG 3713P083 VG P190 DLGG 3714 P084 EVG P191 GLGG 3715 P085 DVG P192 PGG n/aP086 GVG P193 EPGG 3716 P087 PR P194 DPGG 3717 P088 EPR P195 GPGG 3718P089 DPR P196 RGG n/a P090 GPR P197 ERGG 3719 P091 PS P198 DRGG 3720P092 EPS P199 GRGG 3721 P093 DPS P200 SGG n/a P094 GPS P201 ESGG 3722P095 PP P202 DSGG 3723 P096 EPP P203 GSGG 3724 P097 DPP P204 TGG n/aP098 GPP P205 ETGG 3725 P099 PL P206 DTGG 3726 P100 EPL P207 GTGG 3727P101 DPL P208 VGG n/a P102 GPL P209 EVGG 3728 P103 PA P210 DVGG 3729P104 EPA P211 GVGG 3730 P105 DPA P106 GPA

TABLE 11 1K DH Theoretical Segment Pool sequences (1,111 DH segments).DH Segment Amino Acid SEQ Name Sequence ID NO DHUNIV_001 GTTGTT 3731DHUNIV_002 GTTGT 3732 DHUNIV_003 TTGTT 3733 DHUNIV_004 GTTG 3734DHUNIV_005 TTGT 3735 DHUNIV_006 TGTT 3736 DHUNIV_007 GTT n/a DHUNIV_008TTG n/a DHUNIV_009 TGT n/a DHUNIV_010 GT n/a DHUNIV_011 TT n/aDHUNIV_012 TG n/a DHUNIV_013 VQLER 3737 DHUNIV_014 VQLE 3738 DHUNIV_015QLER 3739 DHUNIV_016 VQL n/a DHUNIV_017 QLE n/a DHUNIV_018 LER n/aDHUNIV_019 VQ n/a DHUNIV_020 QL n/a DHUNIV_021 LE n/a DHUNIV_022 ER n/aDHUNIV_023 YNWND 3740 DHUNIV_024 YNWN 3741 DHUNIV_025 NWND 3742DHUNIV_026 YNW n/a DHUNIV_027 NWN n/a DHUNIV_028 WND n/a DHUNIV_029 YNn/a DHUNIV_030 NW n/a DHUNIV_031 WN n/a DHUNIV_032 ND n/a DHUNIV_033GITGTT 3743 DHUNIV_034 GITGT 3744 DHUNIV_035 ITGTT 3745 DHUNIV_036 GITG3746 DHUNIV_037 ITGT 3747 DHUNIV_038 GIT n/a DHUNIV_039 ITG n/aDHUNIV_040 GI n/a DHUNIV_041 IT n/a DHUNIV_042 GIVGATT 3748 DHUNIV_043GIVGAT 3749 DHUNIV_044 IVGATT 3750 DHUNIV_045 GIVGA 3751 DHUNIV_046IVGAT 3752 DHUNIV_047 VGATT 3753 DHUNIV_048 GIVG 3754 DHUNIV_049 IVGA3755 DHUNIV_050 VGAT 3756 DHUNIV_051 GATT 3757 DHUNIV_052 GIV n/aDHUNIV_053 IVG n/a DHUNIV_054 VGA n/a DHUNIV_055 GAT n/a DHUNIV_056 ATTn/a DHUNIV_057 IV n/a DHUNIV_058 VG n/a DHUNIV_059 GA n/a DHUNIV_060 ATn/a DHUNIV_061 WELL 3758 DHUNIV_062 WEL n/a DHUNIV_063 ELL n/aDHUNIV_064 WE n/a DHUNIV_065 EL n/a DHUNIV_066 LL n/a DHUNIV_067 YSGSYY3759 DHUNIV_068 YSGSY 3760 DHUNIV_069 SGSYY 3761 DHUNIV_070 YSGS 3762DHUNIV_071 SGSY 3763 DHUNIV_072 GSYY 3764 DHUNIV_073 YSG n/a DHUNIV_074SGS n/a DHUNIV_075 GSY n/a DHUNIV_076 SYY n/a DHUNIV_077 YS n/aDHUNIV_078 SG n/a DHUNIV_079 GS n/a DHUNIV_080 SY n/a DHUNIV_081 YY n/aDHUNIV_082 LEL n/a DHUNIV_083 YNWNY 3765 DHUNIV_084 NWNY 3766 DHUNIV_085WNY n/a DHUNIV_086 NY n/a DHUNIV_087 RIL n/a DHUNIV_088 LLL n/aDHUNIV_089 RI n/a DHUNIV_090 IL n/a DHUNIV_091 WW n/a DHUNIV_092GYCSGGSCYS 3767 DHUNIV_093 GYCSGGSCY 3768 DHUNIV_094 YCSGGSCYS 3769DHUNIV_095 GYCSGGSC 3770 DHUNIV_096 YCSGGSCY 3771 DHUNIV_097 CSGGSCYS3772 DHUNIV_098 YCSGGSC 3773 DHUNIV_099 CSGGSCY 3774 DHUNIV_100 CSGGSC3775 DHUNIV_101 SGGS 3776 DHUNIV_102 SGG n/a DHUNIV_103 GGS n/aDHUNIV_104 GY n/a DHUNIV_105 GG n/a DHUNIV_106 DIVVVVAATP 3777DHUNIV_107 DIVVVVAAT 3778 DHUNIV_108 IVVVVAATP 3779 DHUNIV_109 DIVVVVAA3780 DHUNIV_110 IVVVVAAT 3781 DHUNIV_111 VVVVAATP 3782 DHUNIV_112DIVVVVA 3783 DHUNIV_113 IVVVVAA 3784 DHUNIV_114 VVVVAAT 3785 DHUNIV_115VVVAATP 3786 DHUNIV_116 DIVVVV 3787 DHUNIV_117 IVVVVA 3788 DHUNIV_118VVVVAA 3789 DHUNIV_119 VVVAAT 3790 DHUNIV_120 VVAATP 3791 DHUNIV_121DIVVV 3792 DHUNIV_122 IVVVV 3793 DHUNIV_123 VVVVA 3794 DHUNIV_124 VVVAA3795 DHUNIV_125 VVAAT 3796 DHUNIV_126 VAATP 3797 DHUNIV_127 DIVV 3798DHUNIV_128 IVVV 3799 DHUNIV_129 VVVV 3800 DHUNIV_130 VVVA 3801DHUNIV_131 VVAA 3802 DHUNIV_132 VAAT 3803 DHUNIV_133 AATP 3804DHUNIV_134 DIV n/a DHUNIV_135 IVV n/a DHUNIV_136 VVV n/a DHUNIV_137 VVAn/a DHUNIV_138 VAA n/a DHUNIV_139 AAT n/a DHUNIV_140 ATP n/a DHUNIV_141DI n/a DHUNIV_142 VV n/a DHUNIV_143 VA n/a DHUNIV_144 AA n/a DHUNIV_145TP n/a DHUNIV_146 YQLL 3805 DHUNIV_147 YQL n/a DHUNIV_148 QLL n/aDHUNIV_149 YQ n/a DHUNIV_150 GYCSSTSCYA 3806 DHUNIV_151 GYCSSTSCY 3807DHUNIV_152 YCSSTSCYA 3808 DHUNIV_153 GYCSSTSC 3809 DHUNIV_154 YCSSTSCY3810 DHUNIV_155 CSSTSCYA 3811 DHUNIV_156 YCSSTSC 3812 DHUNIV_157 CSSTSCY3813 DHUNIV_158 CSSTSC 3814 DHUNIV_159 SSTS 3815 DHUNIV_160 SST n/aDHUNIV_161 STS n/a DHUNIV_162 SS n/a DHUNIV_163 ST n/a DHUNIV_164 TS n/aDHUNIV_165 YA n/a DHUNIV_166 DIVVVPAAMP 3816 DHUNIV_167 DIVVVPAAM 3817DHUNIV_168 IVVVPAAMP 3818 DHUNIV_169 DIVVVPAA 3819 DHUNIV_170 IVVVPAAM3820 DHUNIV_171 VVVPAAMP 3821 DHUNIV_172 DIVVVPA 3822 DHUNIV_173 IVVVPAA3823 DHUNIV_174 VVVPAAM 3824 DHUNIV_175 VVPAAMP 3825 DHUNIV_176 DIVVVP3826 DHUNIV_177 IVVVPA 3827 DHUNIV_178 VVVPAA 3828 DHUNIV_179 VVPAAM3829 DHUNIV_180 VPAAMP 3830 DHUNIV_181 IVVVP 3831 DHUNIV_182 VVVPA 3832DHUNIV_183 VVPAA 3833 DHUNIV_184 VPAAM 3834 DHUNIV_185 PAAMP 3835DHUNIV_186 VVVP 3836 DHUNIV_187 VVPA 3837 DHUNIV_188 VPAA 3838DHUNIV_189 PAAM 3839 DHUNIV_190 AAMP 3840 DHUNIV_191 VVP n/a DHUNIV_192VPA n/a DHUNIV_193 PAA n/a DHUNIV_194 AAM n/a DHUNIV_195 AMP n/aDHUNIV_196 VP n/a DHUNIV_197 PA n/a DHUNIV_198 AM n/a DHUNIV_199 MP n/aDHUNIV_200 YQLLY 3841 DHUNIV_201 QLLY 3842 DHUNIV_202 LLY n/a DHUNIV_203LY n/a DHUNIV_204 GYCSSTSCYT 3843 DHUNIV_205 YCSSTSCYT 3844 DHUNIV_206CSSTSCYT 3845 DHUNIV_207 YT n/a DHUNIV_208 DIVVVPAAIP 3846 DHUNIV_209DIVVVPAAI 3847 DHUNIV_210 IVVVPAAIP 3848 DHUNIV_211 IVVVPAAI 3849DHUNIV_212 VVVPAAIP 3850 DHUNIV_213 VVVPAAI 3851 DHUNIV_214 VVPAAIP 3852DHUNIV_215 VVPAAI 3853 DHUNIV_216 VPAAIP 3854 DHUNIV_217 VPAAI 3855DHUNIV_218 PAAIP 3856 DHUNIV_219 PAAI 3857 DHUNIV_220 AAIP 3858DHUNIV_221 AAI n/a DHUNIV_222 AIP n/a DHUNIV_223 AI n/a DHUNIV_224 IPn/a DHUNIV_225 WIL n/a DHUNIV_226 WI n/a DHUNIV_227 SILWW 3859DHUNIV_228 SILW 3860 DHUNIV_229 ILWW 3861 DHUNIV_230 SIL n/a DHUNIV_231ILW n/a DHUNIV_232 LWW n/a DHUNIV_233 LLF n/a DHUNIV_234 SI n/aDHUNIV_235 LW n/a DHUNIV_236 LF n/a DHUNIV_237 AYCGGDCYS 3862 DHUNIV_238AYCGGDCY 3863 DHUNIV_239 YCGGDCYS 3864 DHUNIV_240 AYCGGDC 3865DHUNIV_241 YCGGDCY 3866 DHUNIV_242 CGGDCYS 3867 DHUNIV_243 YCGGDC 3868DHUNIV_244 CGGDCY 3869 DHUNIV_245 CGGDC 3870 DHUNIV_246 GGD n/aDHUNIV_247 AY n/a DHUNIV_248 GD n/a DHUNIV_249 HIVVVIAIP 3871 DHUNIV_250HIVVVIAI 3872 DHUNIV_251 IVVVIAIP 3873 DHUNIV_252 HIVVVIA 3874DHUNIV_253 IVVVIAI 3875 DHUNIV_254 VVVIAIP 3876 DHUNIV_255 HIVVVI 3877DHUNIV_256 IVVVIA 3878 DHUNIV_257 VVVIAI 3879 DHUNIV_258 VVIAIP 3880DHUNIV_259 HIVVV 3881 DHUNIV_260 IVVVI 3882 DHUNIV_261 VVVIA 3883DHUNIV_262 VVIAI 3884 DHUNIV_263 VIAIP 3885 DHUNIV_264 HIVV 3886DHUNIV_265 VVVI 3887 DHUNIV_266 VVIA 3888 DHUNIV_267 VIAI 3889DHUNIV_268 IAIP 3890 DHUNIV_269 HIV n/a DHUNIV_270 VVI n/a DHUNIV_271VIA n/a DHUNIV_272 IAI n/a DHUNIV_273 HI n/a DHUNIV_274 VI n/aDHUNIV_275 IA n/a DHUNIV_276 HIVVVTAIP 3891 DHUNIV_277 HIVVVTAI 3892DHUNIV_278 IVVVTAIP 3893 DHUNIV_279 HIVVVTA 3894 DHUNIV_280 IVVVTAI 3895DHUNIV_281 VVVTAIP 3896 DHUNIV_282 HIVVVT 3897 DHUNIV_283 IVVVTA 3898DHUNIV_284 VVVTAI 3899 DHUNIV_285 VVTAIP 3900 DHUNIV_286 IVVVT 3901DHUNIV_287 VVVTA 3902 DHUNIV_288 VVTAI 3903 DHUNIV_289 VTAIP 3904DHUNIV_290 VVVT 3905 DHUNIV_291 VVTA 3906 DHUNIV_292 VTAI 3907DHUNIV_293 TAIP 3908 DHUNIV_294 VVT n/a DHUNIV_295 VTA n/a DHUNIV_296TAI n/a DHUNIV_297 VT n/a DHUNIV_298 TA n/a DHUNIV_299 RILY 3909DHUNIV_300 ILY n/a DHUNIV_301 MLY n/a DHUNIV_302 ML n/a DHUNIV_303GYCTNGVCYT 3910 DHUNIV_304 GYCTNGVCY 3911 DHUNIV_305 YCTNGVCYT 3912DHUNIV_306 GYCTNGVC 3913 DHUNIV_307 YCTNGVCY 3914 DHUNIV_308 CTNGVCYT3915 DHUNIV_309 YCTNGVC 3916 DHUNIV_310 CTNGVCY 3917 DHUNIV_311 CTNGVC3918 DHUNIV_312 TNGV 3919 DHUNIV_313 TNG n/a DHUNIV_314 NGV n/aDHUNIV_315 TN n/a DHUNIV_316 NG n/a DHUNIV_317 GV n/a DHUNIV_318DIVLMVYAIP 3920 DHUNIV_319 DIVLMVYAI 3921 DHUNIV_320 IVLMVYAIP 3922DHUNIV_321 DIVLMVYA 3923 DHUNIV_322 IVLMVYAI 3924 DHUNIV_323 VLMVYAIP3925 DHUNIV_324 DIVLMVY 3926 DHUNIV_325 IVLMVYA 3927 DHUNIV_326 VLMVYAI3928 DHUNIV_327 LMVYAIP 3929 DHUNIV_328 DIVLMV 3930 DHUNIV_329 IVLMVY3931 DHUNIV_330 VLMVYA 3932 DHUNIV_331 LMVYAI 3933 DHUNIV_332 MVYAIP3934 DHUNIV_333 DIVLM 3935 DHUNIV_334 IVLMV 3936 DHUNIV_335 VLMVY 3937DHUNIV_336 LMVYA 3938 DHUNIV_337 MVYAI 3939 DHUNIV_338 VYAIP 3940DHUNIV_339 DIVL 3941 DHUNIV_340 IVLM 3942 DHUNIV_341 VLMV 3943DHUNIV_342 LMVY 3944 DHUNIV_343 MVYA 3945 DHUNIV_344 VYAI 3946DHUNIV_345 YAIP 3947 DHUNIV_346 IVL n/a DHUNIV_347 VLM n/a DHUNIV_348LMV n/a DHUNIV_349 MVY n/a DHUNIV_350 VYA n/a DHUNIV_351 YAI n/aDHUNIV_352 VL n/a DHUNIV_353 LM n/a DHUNIV_354 MV n/a DHUNIV_355 VY n/aDHUNIV_356 VLLWFGELL 3948 DHUNIV_357 VLLWFGEL 3949 DHUNIV_358 LLWFGELL3950 DHUNIV_359 VLLWFGE 3951 DHUNIV_360 LLWFGEL 3952 DHUNIV_361 LWFGELL3953 DHUNIV_362 VLLWFG 3954 DHUNIV_363 LLWFGE 3955 DHUNIV_364 LWFGEL3956 DHUNIV_365 WFGELL 3957 DHUNIV_366 VLLWF 3958 DHUNIV_367 LLWFG 3959DHUNIV_368 LWFGE 3960 DHUNIV_369 WFGEL 3961 DHUNIV_370 FGELL 3962DHUNIV_371 VLLW 3963 DHUNIV_372 LLWF 3964 DHUNIV_373 LWFG 3965DHUNIV_374 WFGE 3966 DHUNIV_375 FGEL 3967 DHUNIV_376 GELL 3968DHUNIV_377 VLL n/a DHUNIV_378 LLW n/a DHUNIV_379 LWF n/a DHUNIV_380 WFGn/a DHUNIV_381 FGE n/a DHUNIV_382 GEL n/a DHUNIV_383 WF n/a DHUNIV_384FG n/a DHUNIV_385 GE n/a DHUNIV_386 YYYGSGSYYN 3969 DHUNIV_387 YYYGSGSYY3970 DHUNIV_388 YYGSGSYYN 3971 DHUNIV_389 YYYGSGSY 3972 DHUNIV_390YYGSGSYY 3973 DHUNIV_391 YGSGSYYN 3974 DHUNIV_392 YYYGSGS 3975DHUNIV_393 YYGSGSY 3976 DHUNIV_394 YGSGSYY 3977 DHUNIV_395 GSGSYYN 3978DHUNIV_396 YYYGSG 3979 DHUNIV_397 YYGSGS 3980 DHUNIV_398 YGSGSY 3981DHUNIV_399 GSGSYY 3982 DHUNIV_400 SGSYYN 3983 DHUNIV_401 YYYGS 3984DHUNIV_402 YYGSG 3985 DHUNIV_403 YGSGS 3986 DHUNIV_404 GSGSY 3987DHUNIV_405 GSYYN 3988 DHUNIV_406 YYYG 3989 DHUNIV_407 YYGS 3990DHUNIV_408 YGSG 3991 DHUNIV_409 GSGS 3992 DHUNIV_410 SYYN 3993DHUNIV_411 YYY n/a DHUNIV_412 YYG n/a DHUNIV_413 YGS n/a DHUNIV_414 GSGn/a DHUNIV_415 YYN n/a DHUNIV_416 YG n/a DHUNIV_417 ITMVRGVIIT 3994DHUNIV_418 ITMVRGVII 3995 DHUNIV_419 TMVRGVIIT 3996 DHUNIV_420 ITMVRGVI3997 DHUNIV_421 TMVRGVII 3998 DHUNIV_422 MVRGVIIT 3999 DHUNIV_423ITMVRGV 4000 DHUNIV_424 TMVRGVI 4001 DHUNIV_425 MVRGVII 4002 DHUNIV_426VRGVIIT 4003 DHUNIV_427 ITMVRG 4004 DHUNIV_428 TMVRGV 4005 DHUNIV_429MVRGVI 4006 DHUNIV_430 VRGVII 4007 DHUNIV_431 RGVIIT 4008 DHUNIV_432ITMVR 4009 DHUNIV_433 TMVRG 4010 DHUNIV_434 MVRGV 4011 DHUNIV_435 VRGVI4012 DHUNIV_436 RGVII 4013 DHUNIV_437 GVIIT 4014 DHUNIV_438 ITMV 4015DHUNIV_439 TMVR 4016 DHUNIV_440 MVRG 4017 DHUNIV_441 VRGV 4018DHUNIV_442 RGVI 4019 DHUNIV_443 GVII 4020 DHUNIV_444 VIIT 4021DHUNIV_445 ITM n/a DHUNIV_446 TMV n/a DHUNIV_447 MVR n/a DHUNIV_448 VRGn/a DHUNIV_449 RGV n/a DHUNIV_450 GVI n/a DHUNIV_451 VII n/a DHUNIV_452IIT n/a DHUNIV_453 TM n/a DHUNIV_454 VR n/a DHUNIV_455 RG n/a DHUNIV_456II n/a DHUNIV_457 VLLWFRELL 4022 DHUNIV_458 VLLWFREL 4023 DHUNIV_459LLWFRELL 4024 DHUNIV_460 VLLWFRE 4025 DHUNIV_461 LLWFREL 4026 DHUNIV_462LWFRELL 4027 DHUNIV_463 VLLWFR 4028 DHUNIV_464 LLWFRE 4029 DHUNIV_465LWFREL 4030 DHUNIV_466 WFRELL 4031 DHUNIV_467 LLWFR 4032 DHUNIV_468LWFRE 4033 DHUNIV_469 WFREL 4034 DHUNIV_470 FRELL 4035 DHUNIV_471 LWFR4036 DHUNIV_472 WFRE 4037 DHUNIV_473 FREL 4038 DHUNIV_474 RELL 4039DHUNIV_475 WFR n/a DHUNIV_476 FRE n/a DHUNIV_477 REL n/a DHUNIV_478 FRn/a DHUNIV_479 RE n/a DHUNIV_480 ITMVQGVIIT 4040 DHUNIV_481 ITMVQGVII4041 DHUNIV_482 TMVQGVIIT 4042 DHUNIV_483 ITMVQGVI 4043 DHUNIV_484TMVQGVII 4044 DHUNIV_485 MVQGVIIT 4045 DHUNIV_486 ITMVQGV 4046DHUNIV_487 TMVQGVI 4047 DHUNIV_488 MVQGVII 4048 DHUNIV_489 VQGVIIT 4049DHUNIV_490 ITMVQG 4050 DHUNIV_491 TMVQGV 4051 DHUNIV_492 MVQGVI 4052DHUNIV_493 VQGVII 4053 DHUNIV_494 QGVIIT 4054 DHUNIV_495 ITMVQ 4055DHUNIV_496 TMVQG 4056 DHUNIV_497 MVQGV 4057 DHUNIV_498 VQGVI 4058DHUNIV_499 QGVII 4059 DHUNIV_500 TMVQ 4060 DHUNIV_501 MVQG 4061DHUNIV_502 VQGV 4062 DHUNIV_503 QGVI 4063 DHUNIV_504 MVQ n/a DHUNIV_505VQG n/a DHUNIV_506 QGV n/a DHUNIV_507 QG n/a DHUNIV_508 LRLGEL 4064DHUNIV_509 LRLGE 4065 DHUNIV_510 RLGEL 4066 DHUNIV_511 LRLG 4067DHUNIV_512 RLGE 4068 DHUNIV_513 LGEL 4069 DHUNIV_514 LRL n/a DHUNIV_515RLG n/a DHUNIV_516 LGE n/a DHUNIV_517 LR n/a DHUNIV_518 RL n/aDHUNIV_519 LG n/a DHUNIV_520 YYDYVWGSYAYT 4070 DHUNIV_521 YYDYVWGSYAY4071 DHUNIV_522 YDYVWGSYAYT 4072 DHUNIV_523 YYDYVWGSYA 4073 DHUNIV_524YDYVWGSYAY 4074 DHUNIV_525 DYVWGSYAYT 4075 DHUNIV_526 YYDYVWGSY 4076DHUNIV_527 YDYVWGSYA 4077 DHUNIV_528 DYVWGSYAY 4078 DHUNIV_529 YVWGSYAYT4079 DHUNIV_530 YYDYVWGS 4080 DHUNIV_531 YDYVWGSY 4081 DHUNIV_532DYVWGSYA 4082 DHUNIV_533 YVWGSYAY 4083 DHUNIV_534 VWGSYAYT 4084DHUNIV_535 YYDYVWG 4085 DHUNIV_536 YDYVWGS 4086 DHUNIV_537 DYVWGSY 4087DHUNIV_538 YVWGSYA 4088 DHUNIV_539 VWGSYAY 4089 DHUNIV_540 WGSYAYT 4090DHUNIV_541 YYDYVW 4091 DHUNIV_542 YDYVWG 4092 DHUNIV_543 DYVWGS 4093DHUNIV_544 YVWGSY 4094 DHUNIV_545 VWGSYA 4095 DHUNIV_546 WGSYAY 4096DHUNIV_547 GSYAYT 4097 DHUNIV_548 YYDYV 4098 DHUNIV_549 YDYVW 4099DHUNIV_550 DYVWG 4100 DHUNIV_551 YVWGS 4101 DHUNIV_552 VWGSY 4102DHUNIV_553 WGSYA 4103 DHUNIV_554 GSYAY 4104 DHUNIV_555 SYAYT 4105DHUNIV_556 YYDY 4106 DHUNIV_557 YDYV 4107 DHUNIV_558 DYVW 4108DHUNIV_559 YVWG 4109 DHUNIV_560 VWGS 4110 DHUNIV_561 WGSY 4111DHUNIV_562 GSYA 4112 DHUNIV_563 SYAY 4113 DHUNIV_564 YAYT 4114DHUNIV_565 YYD n/a DHUNIV_566 YDY n/a DHUNIV_567 DYV n/a DHUNIV_568 YVWn/a DHUNIV_569 VWG n/a DHUNIV_570 WGS n/a DHUNIV_571 SYA n/a DHUNIV_572YAY n/a DHUNIV_573 AYT n/a DHUNIV_574 YD n/a DHUNIV_575 DY n/aDHUNIV_576 YV n/a DHUNIV_577 VW n/a DHUNIV_578 WG n/a DHUNIV_579IMITFGGVMLIP 4115 DHUNIV_580 IMITFGGVMLI 4116 DHUNIV_581 MITFGGVMLIP4117 DHUNIV_582 IMITFGGVML 4118 DHUNIV_583 MITFGGVMLI 4119 DHUNIV_584ITFGGVMLIP 4120 DHUNIV_585 IMITFGGVM 4121 DHUNIV_586 MITFGGVML 4122DHUNIV_587 ITFGGVMLI 4123 DHUNIV_588 TFGGVMLIP 4124 DHUNIV_589 IMITFGGV4125 DHUNIV_590 MITFGGVM 4126 DHUNIV_591 ITFGGVML 4127 DHUNIV_592TFGGVMLI 4128 DHUNIV_593 FGGVMLIP 4129 DHUNIV_594 IMITFGG 4130DHUNIV_595 MITFGGV 4131 DHUNIV_596 ITFGGVM 4132 DHUNIV_597 TFGGVML 4133DHUNIV_598 FGGVMLI 4134 DHUNIV_599 GGVMLIP 4135 DHUNIV_600 IMITFG 4136DHUNIV_601 MITFGG 4137 DHUNIV_602 ITFGGV 4138 DHUNIV_603 TFGGVM 4139DHUNIV_604 FGGVML 4140 DHUNIV_605 GGVMLI 4141 DHUNIV_606 GVMLIP 4142DHUNIV_607 IMITF 4143 DHUNIV_608 MITFG 4144 DHUNIV_609 ITFGG 4145DHUNIV_610 TFGGV 4146 DHUNIV_611 FGGVM 4147 DHUNIV_612 GGVML 4148DHUNIV_613 GVMLI 4149 DHUNIV_614 VMLIP 4150 DHUNIV_615 IMIT 4151DHUNIV_616 MITF 4152 DHUNIV_617 ITFG 4153 DHUNIV_618 TFGG 4154DHUNIV_619 FGGV 4155 DHUNIV_620 GGVM 4156 DHUNIV_621 GVML 4157DHUNIV_622 VMLI 4158 DHUNIV_623 MLIP 4159 DHUNIV_624 IMI n/a DHUNIV_625MIT n/a DHUNIV_626 ITF n/a DHUNIV_627 TFG n/a DHUNIV_628 FGG n/aDHUNIV_629 GGV n/a DHUNIV_630 GVM n/a DHUNIV_631 VML n/a DHUNIV_632 MLIn/a DHUNIV_633 LIP n/a DHUNIV_634 IM n/a DHUNIV_635 MI n/a DHUNIV_636 TFn/a DHUNIV_637 VM n/a DHUNIV_638 LI n/a DHUNIV_639 WLLL 4160 DHUNIV_640WLL n/a DHUNIV_641 WL n/a DHUNIV_642 YYYDSSGYYY 4161 DHUNIV_643YYYDSSGYY 4162 DHUNIV_644 YYDSSGYYY 4163 DHUNIV_645 YYYDSSGY 4164DHUNIV_646 YYDSSGYY 4165 DHUNIV_647 YDSSGYYY 4166 DHUNIV_648 YYYDSSG4167 DHUNIV_649 YYDSSGY 4168 DHUNIV_650 YDSSGYY 4169 DHUNIV_651 DSSGYYY4170 DHUNIV_652 YYYDSS 4171 DHUNIV_653 YYDSSG 4172 DHUNIV_654 YDSSGY4173 DHUNIV_655 DSSGYY 4174 DHUNIV_656 SSGYYY 4175 DHUNIV_657 YYYDS 4176DHUNIV_658 YYDSS 4177 DHUNIV_659 YDSSG 4178 DHUNIV_660 DSSGY 4179DHUNIV_661 SSGYY 4180 DHUNIV_662 SGYYY 4181 DHUNIV_663 YYYD 4182DHUNIV_664 YYDS 4183 DHUNIV_665 YDSS 4184 DHUNIV_666 DSSG 4185DHUNIV_667 SSGY 4186 DHUNIV_668 SGYY 4187 DHUNIV_669 GYYY 4188DHUNIV_670 YDS n/a DHUNIV_671 DSS n/a DHUNIV_672 SSG n/a DHUNIV_673 SGYn/a DHUNIV_674 GYY n/a DHUNIV_675 DS n/a DHUNIV_676 ITMIVVVITT 4189DHUNIV_677 ITMIVVVIT 4190 DHUNIV_678 TMIVVVITT 4191 DHUNIV_679 ITMIVVVI4192 DHUNIV_680 TMIVVVIT 4193 DHUNIV_681 MIVVVITT 4194 DHUNIV_682ITMIVVV 4195 DHUNIV_683 TMIVVVI 4196 DHUNIV_684 MIVVVIT 4197 DHUNIV_685IVVVITT 4198 DHUNIV_686 ITMIVV 4199 DHUNIV_687 TMIVVV 4200 DHUNIV_688MIVVVI 4201 DHUNIV_689 IVVVIT 4202 DHUNIV_690 VVVITT 4203 DHUNIV_691ITMIV 4204 DHUNIV_692 TMIVV 4205 DHUNIV_693 MIVVV 4206 DHUNIV_694 VVVIT4207 DHUNIV_695 VVITT 4208 DHUNIV_696 ITMI 4209 DHUNIV_697 TMIV 4210DHUNIV_698 MIVV 4211 DHUNIV_699 VVIT 4212 DHUNIV_700 VITT 4213DHUNIV_701 TMI n/a DHUNIV_702 MIV n/a DHUNIV_703 VIT n/a DHUNIV_704 ITTn/a DHUNIV_705 VLRFLEWLLY 4214 DHUNIV_706 VLRFLEWLL 4215 DHUNIV_707LRFLEWLLY 4216 DHUNIV_708 VLRFLEWL 4217 DHUNIV_709 LRFLEWLL 4218DHUNIV_710 RFLEWLLY 4219 DHUNIV_711 VLRFLEW 4220 DHUNIV_712 LRFLEWL 4221DHUNIV_713 RFLEWLL 4222 DHUNIV_714 FLEWLLY 4223 DHUNIV_715 VLRFLE 4224DHUNIV_716 LRFLEW 4225 DHUNIV_717 RFLEWL 4226 DHUNIV_718 FLEWLL 4227DHUNIV_719 LEWLLY 4228 DHUNIV_720 VLRFL 4229 DHUNIV_721 LRFLE 4230DHUNIV_722 RFLEW 4231 DHUNIV_723 FLEWL 4232 DHUNIV_724 LEWLL 4233DHUNIV_725 EWLLY 4234 DHUNIV_726 VLRF 4235 DHUNIV_727 LRFL 4236DHUNIV_728 RFLE 4237 DHUNIV_729 FLEW 4238 DHUNIV_730 LEWL 4239DHUNIV_731 EWLL 4240 DHUNIV_732 WLLY 4241 DHUNIV_733 VLR n/a DHUNIV_734LRF n/a DHUNIV_735 RFL n/a DHUNIV_736 FLE n/a DHUNIV_737 LEW n/aDHUNIV_738 EWL n/a DHUNIV_739 RF n/a DHUNIV_740 FL n/a DHUNIV_741 EW n/aDHUNIV_742 YYDFWSGYYT 4242 DHUNIV_743 YYDFWSGYY 4243 DHUNIV_744YDFWSGYYT 4244 DHUNIV_745 YYDFWSGY 4245 DHUNIV_746 YDFWSGYY 4246DHUNIV_747 DFWSGYYT 4247 DHUNIV_748 YYDFWSG 4248 DHUNIV_749 YDFWSGY 4249DHUNIV_750 DFWSGYY 4250 DHUNIV_751 FWSGYYT 4251 DHUNIV_752 YYDFWS 4252DHUNIV_753 YDFWSG 4253 DHUNIV_754 DFWSGY 4254 DHUNIV_755 FWSGYY 4255DHUNIV_756 WSGYYT 4256 DHUNIV_757 YYDFW 4257 DHUNIV_758 YDFWS 4258DHUNIV_759 DFWSG 4259 DHUNIV_760 FWSGY 4260 DHUNIV_761 WSGYY 4261DHUNIV_762 SGYYT 4262 DHUNIV_763 YYDF 4263 DHUNIV_764 YDFW 4264DHUNIV_765 DFWS 4265 DHUNIV_766 FWSG 4266 DHUNIV_767 WSGY 4267DHUNIV_768 GYYT 4268 DHUNIV_769 YDF n/a DHUNIV_770 DFW n/a DHUNIV_771FWS n/a DHUNIV_772 WSG n/a DHUNIV_773 YYT n/a DHUNIV_774 DF n/aDHUNIV_775 FW n/a DHUNIV_776 WS n/a DHUNIV_777 ITIFGVVIIP 4269DHUNIV_778 ITIFGVVII 4270 DHUNIV_779 TIFGVVIIP 4271 DHUNIV_780 ITIFGVVI4272 DHUNIV_781 TIFGVVII 4273 DHUNIV_782 IFGVVIIP 4274 DHUNIV_783ITIFGVV 4275 DHUNIV_784 TIFGVVI 4276 DHUNIV_785 IFGVVII 4277 DHUNIV_786FGVVIIP 4278 DHUNIV_787 ITIFGV 4279 DHUNIV_788 TIFGVV 4280 DHUNIV_789IFGVVI 4281 DHUNIV_790 FGVVII 4282 DHUNIV_791 GVVIIP 4283 DHUNIV_792ITIFG 4284 DHUNIV_793 TIFGV 4285 DHUNIV_794 IFGVV 4286 DHUNIV_795 FGVVI4287 DHUNIV_796 GVVII 4288 DHUNIV_797 VVIIP 4289 DHUNIV_798 ITIF 4290DHUNIV_799 TIFG 4291 DHUNIV_800 IFGV 4292 DHUNIV_801 FGVV 4293DHUNIV_802 GVVI 4294 DHUNIV_803 VVII 4295 DHUNIV_804 VIIP 4296DHUNIV_805 ITI n/a DHUNIV_806 TIF n/a DHUNIV_807 IFG n/a DHUNIV_808 FGVn/a DHUNIV_809 GVV n/a DHUNIV_810 IIP n/a DHUNIV_811 TI n/a DHUNIV_812IF n/a DHUNIV_813 VLRYFDWLL 4297 DHUNIV_814 VLRYFDWL 4298 DHUNIV_815LRYFDWLL 4299 DHUNIV_816 VLRYFDW 4300 DHUNIV_817 LRYFDWL 4301 DHUNIV_818RYFDWLL 4302 DHUNIV_819 VLRYFD 4303 DHUNIV_820 LRYFDW 4304 DHUNIV_821RYFDWL 4305 DHUNIV_822 YFDWLL 4306 DHUNIV_823 VLRYF 4307 DHUNIV_824LRYFD 4308 DHUNIV_825 RYFDW 4309 DHUNIV_826 YFDWL 4310 DHUNIV_827 FDWLL4311 DHUNIV_828 VLRY 4312 DHUNIV_829 LRYF 4313 DHUNIV_830 RYFD 4314DHUNIV_831 YFDW 4315 DHUNIV_832 FDWL 4316 DHUNIV_833 DWLL 4317DHUNIV_834 LRY n/a DHUNIV_835 RYF n/a DHUNIV_836 YFD n/a DHUNIV_837 FDWn/a DHUNIV_838 DWL n/a DHUNIV_839 RY n/a DHUNIV_840 YF n/a DHUNIV_841 FDn/a DHUNIV_842 DW n/a DHUNIV_843 YYDILTGYYN 4318 DHUNIV_844 YYDILTGYY4319 DHUNIV_845 YDILTGYYN 4320 DHUNIV_846 YYDILTGY 4321 DHUNIV_847YDILTGYY 4322 DHUNIV_848 DILTGYYN 4323 DHUNIV_849 YYDILTG 4324DHUNIV_850 YDILTGY 4325 DHUNIV_851 DILTGYY 4326 DHUNIV_852 ILTGYYN 4327DHUNIV_853 YYDILT 4328 DHUNIV_854 YDILTG 4329 DHUNIV_855 DILTGY 4330DHUNIV_856 ILTGYY 4331 DHUNIV_857 LTGYYN 4332 DHUNIV_858 YYDIL 4333DHUNIV_859 YDILT 4334 DHUNIV_860 DILTG 4335 DHUNIV_861 ILTGY 4336DHUNIV_862 LTGYY 4337 DHUNIV_863 TGYYN 4338 DHUNIV_864 YYDI 4339DHUNIV_865 YDIL 4340 DHUNIV_866 DILT 4341 DHUNIV_867 ILTG 4342DHUNIV_868 LTGY 4343 DHUNIV_869 TGYY 4344 DHUNIV_870 GYYN 4345DHUNIV_871 YDI n/a DHUNIV_872 DIL n/a DHUNIV_873 ILT n/a DHUNIV_874 LTGn/a DHUNIV_875 TGY n/a DHUNIV_876 LT n/a DHUNIV_877 LVIIT 4346DHUNIV_878 LVII 4347 DHUNIV_879 LVI n/a DHUNIV_880 LV n/a DHUNIV_881DYGDY 4348 DHUNIV_882 DYGD 4349 DHUNIV_883 YGDY 4350 DHUNIV_884 DYG n/aDHUNIV_885 YGD n/a DHUNIV_886 GDY n/a DHUNIV_887 TTVTT 4351 DHUNIV_888TTVT 4352 DHUNIV_889 TVTT 4353 DHUNIV_890 TTV n/a DHUNIV_891 TVT n/aDHUNIV_892 VTT n/a DHUNIV_893 TV n/a DHUNIV_894 LRW n/a DHUNIV_895 RWn/a DHUNIV_896 DYGGNS 4354 DHUNIV_897 DYGGN 4355 DHUNIV_898 YGGNS 4356DHUNIV_899 DYGG 4357 DHUNIV_900 YGGN 4358 DHUNIV_901 GGNS 4359DHUNIV_902 YGG n/a DHUNIV_903 GGN n/a DHUNIV_904 GNS n/a DHUNIV_905 GNn/a DHUNIV_906 NS n/a DHUNIV_907 TTVVTP 4360 DHUNIV_908 TTVVT 4361DHUNIV_909 TVVTP 4362 DHUNIV_910 TTVV 4363 DHUNIV_911 TVVT 4364DHUNIV_912 VVTP 4365 DHUNIV_913 TVV n/a DHUNIV_914 VTP n/a DHUNIV_915 LQn/a DHUNIV_916 DYSNY 4366 DHUNIV_917 DYSN 4367 DHUNIV_918 YSNY 4368DHUNIV_919 DYS n/a DHUNIV_920 YSN n/a DHUNIV_921 SNY n/a DHUNIV_922 SNn/a DHUNIV_923 VDIVATIT 4369 DHUNIV_924 VDIVATI 4370 DHUNIV_925 DIVATIT4371 DHUNIV_926 VDIVAT 4372 DHUNIV_927 DIVATI 4373 DHUNIV_928 IVATIT4374 DHUNIV_929 VDIVA 4375 DHUNIV_930 DIVAT 4376 DHUNIV_931 IVATI 4377DHUNIV_932 VATIT 4378 DHUNIV_933 VDIV 4379 DHUNIV_934 DIVA 4380DHUNIV_935 IVAT 4381 DHUNIV_936 VATI 4382 DHUNIV_937 ATIT 4383DHUNIV_938 VDI n/a DHUNIV_939 IVA n/a DHUNIV_940 VAT n/a DHUNIV_941 ATIn/a DHUNIV_942 TIT n/a DHUNIV_943 VD n/a DHUNIV_944 WLRL 4384 DHUNIV_945WLR n/a DHUNIV_946 GYSGYDY 4385 DHUNIV_947 GYSGYD 4386 DHUNIV_948 YSGYDY4387 DHUNIV_949 GYSGY 4388 DHUNIV_950 YSGYD 4389 DHUNIV_951 SGYDY 4390DHUNIV_952 GYSG 4391 DHUNIV_953 YSGY 4392 DHUNIV_954 SGYD 4393DHUNIV_955 GYDY 4394 DHUNIV_956 GYS n/a DHUNIV_957 GYD n/a DHUNIV_958VEMATIT 4395 DHUNIV_959 VEMATI 4396 DHUNIV_960 EMATIT 4397 DHUNIV_961VEMAT 4398 DHUNIV_962 EMATI 4399 DHUNIV_963 MATIT 4400 DHUNIV_964 VEMA4401 DHUNIV_965 EMAT 4402 DHUNIV_966 MATI 4403 DHUNIV_967 VEM n/aDHUNIV_968 EMA n/a DHUNIV_969 MAT n/a DHUNIV_970 VE n/a DHUNIV_971 EMn/a DHUNIV_972 MA n/a DHUNIV_973 RWLQL 4404 DHUNIV_974 RWLQ 4405DHUNIV_975 WLQL 4406 DHUNIV_976 RWL n/a DHUNIV_977 WLQ n/a DHUNIV_978LQL n/a DHUNIV_979 RDGYNY 4407 DHUNIV_980 RDGYN 4408 DHUNIV_981 DGYNY4409 DHUNIV_982 RDGY 4410 DHUNIV_983 DGYN 4411 DHUNIV_984 GYNY 4412DHUNIV_985 RDG n/a DHUNIV_986 DGY n/a DHUNIV_987 GYN n/a DHUNIV_988 YNYn/a DHUNIV_989 RD n/a DHUNIV_990 DG n/a DHUNIV_991 VDTAMVT 4413DHUNIV_992 VDTAMV 4414 DHUNIV_993 DTAMVT 4415 DHUNIV_994 VDTAM 4416DHUNIV_995 DTAMV 4417 DHUNIV_996 TAMVT 4418 DHUNIV_997 VDTA 4419DHUNIV_998 DTAM 4420 DHUNIV_999 TAMV 4421 DHUNIV_1000 AMVT 4422DHUNIV_1001 VDT n/a DHUNIV_1002 DTA n/a DHUNIV_1003 TAM n/a DHUNIV_1004AMV n/a DHUNIV_1005 MVT n/a DHUNIV_1006 DT n/a DHUNIV_1007 WIQLWL 4423DHUNIV_1008 WIQLW 4424 DHUNIV_1009 IQLWL 4425 DHUNIV_1010 WIQL 4426DHUNIV_1011 IQLW 4427 DHUNIV_1012 QLWL 4428 DHUNIV_1013 WIQ n/aDHUNIV_1014 IQL n/a DHUNIV_1015 QLW n/a DHUNIV_1016 LWL n/a DHUNIV_1017IQ n/a DHUNIV_1018 GYSYGY 4429 DHUNIV_1019 GYSYG 4430 DHUNIV_1020 YSYGY4431 DHUNIV_1021 GYSY 4432 DHUNIV_1022 YSYG 4433 DHUNIV_1023 SYGY 4434DHUNIV_1024 YSY n/a DHUNIV_1025 SYG n/a DHUNIV_1026 YGY n/a DHUNIV_1027GYSSSWY 4435 DHUNIV_1028 GYSSSW 4436 DHUNIV_1029 YSSSWY 4437 DHUNIV_1030GYSSS 4438 DHUNIV_1031 YSSSW 4439 DHUNIV_1032 SSSWY 4440 DHUNIV_1033GYSS 4441 DHUNIV_1034 YSSS 4442 DHUNIV_1035 SSSW 4443 DHUNIV_1036 SSWY4444 DHUNIV_1037 YSS n/a DHUNIV_1038 SSS n/a DHUNIV_1039 SSW n/aDHUNIV_1040 SWY n/a DHUNIV_1041 SW n/a DHUNIV_1042 WY n/a DHUNIV_1043GIAAAGT 4445 DHUNIV_1044 GIAAAG 4446 DHUNIV_1045 IAAAGT 4447 DHUNIV_1046GIAAA 4448 DHUNIV_1047 IAAAG 4449 DHUNIV_1048 AAAGT 4450 DHUNIV_1049GIAA 4451 DHUNIV_1050 IAAA 4452 DHUNIV_1051 AAAG 4453 DHUNIV_1052 AAGT4454 DHUNIV_1053 GIA n/a DHUNIV_1054 IAA n/a DHUNIV_1055 AAA n/aDHUNIV_1056 AAG n/a DHUNIV_1057 AGT n/a DHUNIV_1058 AG n/a DHUNIV_1059QQLV 4455 DHUNIV_1060 QQL n/a DHUNIV_1061 QLV n/a DHUNIV_1062 QQ n/aDHUNIV_1063 GYSSGWY 4456 DHUNIV_1064 GYSSGW 4457 DHUNIV_1065 YSSGWY 4458DHUNIV_1066 GYSSG 4459 DHUNIV_1067 YSSGW 4460 DHUNIV_1068 SSGWY 4461DHUNIV_1069 YSSG 4462 DHUNIV_1070 SSGW 4463 DHUNIV_1071 SGWY 4464DHUNIV_1072 SGW n/a DHUNIV_1073 GWY n/a DHUNIV_1074 GW n/a DHUNIV_1075GIAVAGT 4465 DHUNIV_1076 GIAVAG 4466 DHUNIV_1077 IAVAGT 4467 DHUNIV_1078GIAVA 4468 DHUNIV_1079 IAVAG 4469 DHUNIV_1080 AVAGT 4470 DHUNIV_1081GIAV 4471 DHUNIV_1082 IAVA 4472 DHUNIV_1083 AVAG 4473 DHUNIV_1084 VAGT4474 DHUNIV_1085 IAV n/a DHUNIV_1086 AVA n/a DHUNIV_1087 VAG n/aDHUNIV_1088 AV n/a DHUNIV_1089 QWLV 4475 DHUNIV_1090 QWL n/a DHUNIV_1091WLV n/a DHUNIV_1092 QW n/a DHUNIV_1093 EYSSSS 4476 DHUNIV_1094 EYSSS4477 DHUNIV_1095 YSSSS 4478 DHUNIV_1096 EYSS 4479 DHUNIV_1097 SSSS 4480DHUNIV_1098 EYS n/a DHUNIV_1099 EY n/a DHUNIV_1100 SIAARP 4481DHUNIV_1101 SIAAR 4482 DHUNIV_1102 IAARP 4483 DHUNIV_1103 SIAA 4484DHUNIV_1104 IAAR 4485 DHUNIV_1105 AARP 4486 DHUNIV_1106 SIA n/aDHUNIV_1107 AAR n/a DHUNIV_1108 ARP n/a DHUNIV_1109 AR n/a DHUNIV_1110RP n/a DHUNIV_1111 NWG n/a

TABLE 12 Theoretical segment pool of 141 N2 segments inTheoretical Segment Pool 1 (TSP1). Segment Type Sequences Number “Zero”(no addition) V segment joins 1 directly to D segment MonomersG, P, R, A, S, L, T, V, D, E, 18 F, H, I, K, M, Q, W, Y DimersGG, GP, GR, GA, GS, GL, GT, 82 GV, PG, RG, AG, SG, LG, TG,VG, PP, PR, PA, PS, PL, PT, PV, RP, AP, SP, LP, TP, VP,AR, AS, AT, AY, DL, DT, EA, EK, FH, FS, HL, HW, IS, KV,LD, LE, LR, LS, LT, NR, NT, QE, QL, QT, RA, RD, RE, RF,RH, RL, RR, RS, RV, SA, SD, SE, SF, SI, SK, SL, SQ, SR,SS, ST, SV, TA, TR, TS, TT, TW, VD, VS, WS, YS TrimersGGG, GPG, GRG, GAG, GSG, GLG, 40 GTG, GVG, PGG, RGG, AGG, SGG,LGG, TGG, VGG, GGP, GGR, GGA, GGS, GGL, GGT, GGV , AAE, AYH,DTL, EKR, ISR, NTP, PKS, PRP, PTA, PTQ, REL, RPL, SAA, SAL,SGL, SSE, TGL, WGT

TABLE 13 Theoretical segment pool of 285 H3-JH segments. H3-JH SegmentAmino Acid Name Sequence SEQ ID NO JHUNIV_001 TEYFQH 4487 JHUNIV_002EYFQH 4488 JHUNIV_003 YFQH 4489 JHUNIV_004 FQH n/a JHUNIV_005 QH n/aJHUNIV_006 H n/a JHUNIV_007 n/a JHUNIV_008 SEYFQH 4490 JHUNIV_009 PEYFQH4491 JHUNIV_010 FEYFQH 4492 JHUNIV_011 HEYFQH 4493 JHUNIV_012 REYFQH4494 JHUNIV_013 LEYFQH 4495 JHUNIV_014 NEYFQH 4496 JHUNIV_015 IEYFQH4497 JHUNIV_016 DEYFQH 4498 JHUNIV_017 GEYFQH 4499 JHUNIV_018 VEYFQH4500 JHUNIV_019 YEYFQH 4501 JHUNIV_020 KYFQH 4502 JHUNIV_021 QYFQH 4503JHUNIV_022 LYFQH 4504 JHUNIV_023 SYFQH 4505 JHUNIV_024 RYFQH 4506JHUNIV_025 PYFQH 4507 JHUNIV_026 IYFQH 4508 JHUNIV_027 TYFQH 4509JHUNIV_028 GYFQH 4510 JHUNIV_029 VYFQH 4511 JHUNIV_030 AYFQH 4512JHUNIV_031 NFQH 4513 JHUNIV_032 DFQH 4514 JHUNIV_033 HFQH 4515JHUNIV_034 FFQH 4516 JHUNIV_035 SFQH 4517 JHUNIV_036 RFQH 4518JHUNIV_037 LFQH 4519 JHUNIV_038 PFQH 4520 JHUNIV_039 IFQH 4521JHUNIV_040 TFQH 4522 JHUNIV_041 GFQH 4523 JHUNIV_042 VFQH 4524JHUNIV_043 AFQH 4525 JHUNIV_044 AEYFQH 4526 JHUNIV_045 YWYFDL 4527JHUNIV_046 WYFDL 4528 JHUNIV_047 YFDL 4529 JHUNIV_048 FDL n/a JHUNIV_049DL n/a JHUNIV_050 L n/a JHUNIV_051 DWYFDL 4530 JHUNIV_052 HWYFDL 4531JHUNIV_053 NWYFDL 4532 JHUNIV_054 GYFDL 4533 JHUNIV_055 RYFDL 4534JHUNIV_056 HFDL 4535 JHUNIV_057 NFDL 4536 JHUNIV_058 DFDL 4537JHUNIV_059 DAFDI 4538 JHUNIV_060 AFDI 4539 JHUNIV_061 FDI n/a JHUNIV_062DI n/a JHUNIV_063 I n/a JHUNIV_064 YAFDI 4540 JHUNIV_065 HAFDI 4541JHUNIV_066 FAFDI 4542 JHUNIV_067 SAFDI 4543 JHUNIV_068 RAFDI 4544JHUNIV_069 LAFDI 4545 JHUNIV_070 PAFDI 4546 JHUNIV_071 IAFDI 4547JHUNIV_072 TAFDI 4548 JHUNIV_073 GAFDI 4549 JHUNIV_074 VAFDI 4550JHUNIV_075 AAFDI 4551 JHUNIV_076 TFDI 4552 JHUNIV_077 SFDI 4553JHUNIV_078 PFDI 4554 JHUNIV_079 FFDI 4555 JHUNIV_080 HFDI 4556JHUNIV_081 RFDI 4557 JHUNIV_082 LFDI 4558 JHUNIV_083 NFDI 4559JHUNIV_084 IFDI 4560 JHUNIV_085 DFDI 4561 JHUNIV_086 GFDI 4562JHUNIV_087 VFDI 4563 JHUNIV_088 YFDI 4564 JHUNIV_089 IDI n/a JHUNIV_090VDI n/a JHUNIV_091 LDI n/a JHUNIV_092 SDI n/a JHUNIV_093 HDI n/aJHUNIV_094 RDI n/a JHUNIV_095 PDI n/a JHUNIV_096 NDI n/a JHUNIV_097 TDIn/a JHUNIV_098 DDI n/a JHUNIV_099 GDI n/a JHUNIV_100 ADI n/a JHUNIV_101YDI n/a JHUNIV_102 NAFDI 4565 JHUNIV_103 DYFDY 4566 JHUNIV_104 YFDY 4567JHUNIV_105 FDY n/a JHUNIV_106 DY n/a JHUNIV_107 Y n/a JHUNIV_108 YYFDY4568 JHUNIV_109 HYFDY 4569 JHUNIV_110 FYFDY 4570 JHUNIV_111 SYFDY 4571JHUNIV_112 RYFDY 4572 JHUNIV_113 LYFDY 4573 JHUNIV_114 PYFDY 4574JHUNIV_115 IYFDY 4575 JHUNIV_116 TYFDY 4576 JHUNIV_117 GYFDY 4577JHUNIV_118 VYFDY 4578 JHUNIV_119 AYFDY 4579 JHUNIV_120 NFDY 4580JHUNIV_121 DFDY 4581 JHUNIV_122 HFDY 4582 JHUNIV_123 FFDY 4583JHUNIV_124 SFDY 4584 JHUNIV_125 RFDY 4585 JHUNIV_126 LFDY 4586JHUNIV_127 PFDY 4587 JHUNIV_128 IFDY 4588 JHUNIV_129 TFDY 4589JHUNIV_130 GFDY 4590 JHUNIV_131 VFDY 4591 JHUNIV_132 AFDY 4592JHUNIV_133 IDY n/a JHUNIV_134 VDY n/a JHUNIV_135 LDY n/a JHUNIV_136 SDYn/a JHUNIV_137 HDY n/a JHUNIV_138 RDY n/a JHUNIV_139 PDY n/a JHUNIV_140NDY n/a JHUNIV_141 TDY n/a JHUNIV_142 DDY n/a JHUNIV_143 GDY n/aJHUNIV_144 ADY n/a JHUNIV_145 YDY n/a JHUNIV_146 NYFDY 4593 JHUNIV_147DNWFDP 4594 JHUNIV_148 NWFDP 4595 JHUNIV_149 WFDP 4596 JHUNIV_150 FDPn/a JHUNIV_151 DP n/a JHUNIV_152 P n/a JHUNIV_153 YNWFDP 4597 JHUNIV_154HNWFDP 4598 JHUNIV_155 FNWFDP 4599 JHUNIV_156 SNWFDP 4600 JHUNIV_157RNWFDP 4601 JHUNIV_158 LNWFDP 4602 JHUNIV_159 PNWFDP 4603 JHUNIV_160INWFDP 4604 JHUNIV_161 TNWFDP 4605 JHUNIV_162 GNWFDP 4606 JHUNIV_163VNWFDP 4607 JHUNIV_164 ANWFDP 4608 JHUNIV_165 DWFDP 4609 JHUNIV_166YWFDP 4610 JHUNIV_167 HWFDP 4611 JHUNIV_168 FWFDP 4612 JHUNIV_169 SWFDP4613 JHUNIV_170 RWFDP 4614 JHUNIV_171 LWFDP 4615 JHUNIV_172 PWFDP 4616JHUNIV_173 IWFDP 4617 JHUNIV_174 TWFDP 4618 JHUNIV_175 GWFDP 4619JHUNIV_176 VWFDP 4620 JHUNIV_177 AWFDP 4621 JHUNIV_178 RFDP 4622JHUNIV_179 GFDP 4623 JHUNIV_180 LFDP 4624 JHUNIV_181 SFDP 4625JHUNIV_182 QFDP 4626 JHUNIV_183 PFDP 4627 JHUNIV_184 KFDP 4628JHUNIV_185 MFDP 4629 JHUNIV_186 TFDP 4630 JHUNIV_187 EFDP 4631JHUNIV_188 VFDP 4632 JHUNIV_189 AFDP 4633 JHUNIV_190 NNWFDP 4634JHUNIV_191 DYYYYYGMDV 4635 JHUNIV_192 YYYYYGMDV 4636 JHUNIV_193 YYYYGMDV4637 JHUNIV_194 YYYGMDV 4638 JHUNIV_195 YYGMDV 4639 JHUNIV_196 YGMDV4640 JHUNIV_197 GMDV 4641 JHUNIV_198 MDV n/a JHUNIV_199 DV n/aJHUNIV_200 V n/a JHUNIV_201 YYYYYYGMDV 4642 JHUNIV_202 HYYYYYGMDV 4643JHUNIV_203 FYYYYYGMDV 4644 JHUNIV_204 SYYYYYGMDV 4645 JHUNIV_205RYYYYYGMDV 4646 JHUNIV_206 LYYYYYGMDV 4647 JHUNIV_207 PYYYYYGMDV 4648JHUNIV_208 IYYYYYGMDV 4649 JHUNIV_209 TYYYYYGMDV 4650 JHUNIV_210GYYYYYGMDV 4651 JHUNIV_211 VYYYYYGMDV 4652 JHUNIV_212 AYYYYYGMDV 4653JHUNIV_213 NYYYYGMDV 4654 JHUNIV_214 DYYYYGMDV 4655 JHUNIV_215 HYYYYGMDV4656 JHUNIV_216 FYYYYGMDV 4657 JHUNIV_217 SYYYYGMDV 4658 JHUNIV_218RYYYYGMDV 4659 JHUNIV_219 LYYYYGMDV 4660 JHUNIV_220 PYYYYGMDV 4661JHUNIV_221 IYYYYGMDV 4662 JHUNIV_222 TYYYYGMDV 4663 JHUNIV_223 GYYYYGMDV4664 JHUNIV_224 VYYYYGMDV 4665 JHUNIV_225 AYYYYGMDV 4666 JHUNIV_226NYYYGMDV 4667 JHUNIV_227 DYYYGMDV 4668 JHUNIV_228 HYYYGMDV 4669JHUNIV_229 FYYYGMDV 4670 JHUNIV_230 SYYYGMDV 4671 JHUNIV_231 RYYYGMDV4672 JHUNIV_232 LYYYGMDV 4673 JHUNIV_233 PYYYGMDV 4674 JHUNIV_234IYYYGMDV 4675 JHUNIV_235 TYYYGMDV 4676 JHUNIV_236 GYYYGMDV 4677JHUNIV_237 VYYYGMDV 4678 JHUNIV_238 AYYYGMDV 4679 JHUNIV_239 NYYYYYGMDV4680 JHUNIV_240 DYYYYYYMDV 4681 JHUNIV_241 YYYYYYMDV 4682 JHUNIV_242YYYYYMDV 4683 JHUNIV_243 YYYYMDV 4684 JHUNIV_244 YYYMDV 4685 JHUNIV_245YYMDV 4686 JHUNIV_246 YMDV 4687 JHUNIV_247 YYYYYYYMDV 4688 JHUNIV_248HYYYYYYMDV 4689 JHUNIV_249 FYYYYYYMDV 4690 JHUNIV_250 SYYYYYYMDV 4691JHUNIV_251 RYYYYYYMDV 4692 JHUNIV_252 LYYYYYYMDV 4693 JHUNIV_253PYYYYYYMDV 4694 JHUNIV_254 IYYYYYYMDV 4695 JHUNIV_255 TYYYYYYMDV 4696JHUNIV_256 GYYYYYYMDV 4697 JHUNIV_257 VYYYYYYMDV 4698 JHUNIV_258AYYYYYYMDV 4699 JHUNIV_259 NYYYYYMDV 4700 JHUNIV_260 DYYYYYMDV 4701JHUNIV_261 HYYYYYMDV 4702 JHUNIV_262 FYYYYYMDV 4703 JHUNIV_263 SYYYYYMDV4704 JHUNIV_264 RYYYYYMDV 4705 JHUNIV_265 LYYYYYMDV 4706 JHUNIV_266PYYYYYMDV 4707 JHUNIV_267 IYYYYYMDV 4708 JHUNIV_268 TYYYYYMDV 4709JHUNIV_269 GYYYYYMDV 4710 JHUNIV_270 VYYYYYMDV 4711 JHUNIV_271 AYYYYYMDV4712 JHUNIV_272 NYYYYMDV 4713 JHUNIV_273 DYYYYMDV 4714 JHUNIV_274HYYYYMDV 4715 JHUNIV_275 FYYYYMDV 4716 JHUNIV_276 SYYYYMDV 4717JHUNIV_277 RYYYYMDV 4718 JHUNIV_278 LYYYYMDV 4719 JHUNIV_279 PYYYYMDV4720 JHUNIV_280 IYYYYMDV 4721 JHUNIV_281 TYYYYMDV 4722 JHUNIV_282GYYYYMDV 4723 JHUNIV_283 VYYYYMDV 4724 JHUNIV_284 AYYYYMDV 4725JHUNIV_285 NYYYYYYMDV 4726

TABLE 14 Twelve germline IGHJ genes and alleles. IGHJ Gene DNA SequenceSEQ ID NO IGHJ1-01 GCTGAATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG4727 IGHJ2-01 CTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG 4728IGHJ3-01 ATGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 4729 IGHJ3-02ATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 4730 IGHJ4-01ACTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 4731 IGHJ4-02ACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4732 IGHJ4-03GCTACTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 4733 IGHJ5-01ACAACTGGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 4734 IGHJ5-02ACAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4735 IGHJ6-01ATTACTACTACTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCT 4736 CCTCAGIGHJ6-02 ATTACTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT 4737CCTCAG IGHJ6-03ATTACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCT 4738 CCTCAG

TABLE 15 Theoretical segment pool of 248 parent H3-JH segments. H3-JHParent Segment Amino Acid Name Sequence SEQ ID NO JHparent001 ADI n/aJHparent002 ADY n/a JHparent003 DDI n/a JHparent004 DDY n/a JHparent005GDI n/a JHparent006 GDY n/a JHparent007 HDI n/a JHparent008 HDY n/aJHparent009 IDI n/a JHparent010 IDY n/a JHparent011 LDI n/a JHparent012LDY n/a JHparent013 NDI n/a JHparent014 NDY n/a JHparent015 PDI n/aJHparent016 PDY n/a JHparent017 RDI n/a JHparent018 RDY n/a JHparent019SDI n/a JHparent020 SDY n/a JHparent021 TDI n/a JHparent022 TDY n/aJHparent023 VDI n/a JHparent024 VDY n/a JHparent025 YDI n/a JHparent026YDY n/a JHparent027 AFDP 4633 JHparent028 AFDY 4592 JHparent029 AFQH4525 JHparent030 DFDI 4561 JHparent031 DFDL 4537 JHparent032 DFDY 4581JHparent033 DFQH 4514 JHparent034 EFDP 4631 JHparent035 FFDI 4555JHparent036 FFDY 4583 JHparent037 FFQH 4516 JHparent038 GFDI 4562JHparent039 GFDP 4623 JHparent040 GFDY 4590 JHparent041 GFQH 4523JHparent042 HFDI 4556 JHparent043 HFDL 4535 JHparent044 HFDY 4582JHparent045 HFQH 4515 JHparent046 IFDI 4560 JHparent047 IFDY 4588JHparent048 IFQH 4521 JHparent049 KFDP 4628 JHparent050 LFDI 4558JHparent051 LFDP 4624 JHparent052 LFDY 4586 JHparent053 LFQH 4519JHparent054 MFDP 4629 JHparent055 NFDI 4559 JHparent056 NFDL 4536JHparent057 NFDY 4580 JHparent058 NFQH 4513 JHparent059 PFDI 4554JHparent060 PFDP 4627 JHparent061 PFDY 4587 JHparent062 PFQH 4520JHparent063 QFDP 4626 JHparent064 RFDI 4557 JHparent065 RFDP 4622JHparent066 RFDY 4585 JHparent067 RFQH 4518 JHparent068 SFDI 4553JHparent069 SFDP 4625 JHparent070 SFDY 4584 JHparent071 SFQH 4517JHparent072 TFDI 4552 JHparent073 TFDP 4630 JHparent074 TFDY 4589JHparent075 TFQH 4522 JHparent076 VFDI 4563 JHparent077 VFDP 4632JHparent078 VFDY 4591 JHparent079 VFQH 4524 JHparent080 YFDI 4564JHparent081 YFDL 4529 JHparent082 AAFDI 4551 JHparent083 AWFDP 4621JHparent084 AYFDY 4579 JHparent085 AYFQH 4512 JHparent086 DAFDI 4538JHparent087 DWFDP 4609 JHparent088 DYFDY 4566 JHparent089 FAFDI 4542JHparent090 FWFDP 4612 JHparent091 FYFDY 4570 JHparent092 GAFDI 4549JHparent093 GWFDP 4619 JHparent094 GYFDL 4533 JHparent095 GYFDY 4577JHparent096 GYFQH 4510 JHparent097 HAFDI 4541 JHparent098 HWFDP 4611JHparent099 HYFDY 4569 JHparent100 IAFDI 4547 JHparent101 IWFDP 4617JHparent102 IYFDY 4575 JHparent103 IYFQH 4508 JHparent104 KYFQH 4502JHparent105 LAFDI 4545 JHparent106 LWFDP 4615 JHparent107 LYFDY 4573JHparent108 LYFQH 4504 JHparent109 NAFDI 4565 JHparent110 NYFDY 4593JHparent111 PAFDI 4546 JHparent112 PWFDP 4616 JHparent113 PYFDY 4574JHparent114 PYFQH 4507 JHparent115 QYFQH 4503 JHparent116 RAFDI 4544JHparent117 RWFDP 4614 JHparent118 RYFDL 4534 JHparent119 RYFDY 4572JHparent120 RYFQH 4506 JHparent121 SAFDI 4543 JHparent122 SWFDP 4613JHparent123 SYFDY 4571 JHparent124 SYFQH 4505 JHparent125 TAFDI 4548JHparent126 TWFDP 4618 JHparent127 TYFDY 4576 JHparent128 TYFQH 4509JHparent129 VAFDI 4550 JHparent130 VWFDP 4620 JHparent131 VYFDY 4578JHparent132 VYFQH 4511 JHparent133 WYFDL 4528 JHparent134 YAFDI 4540JHparent135 YWFDP 4610 JHparent136 YYFDY 4568 JHparent137 AEYFQH 4526JHparent138 ANWFDP 4608 JHparent139 DEYFQH 4498 JHparent140 DNWFDP 4594JHparent141 DWYFDL 4530 JHparent142 FEYFQH 4492 JHparent143 FNWFDP 4599JHparent144 GEYFQH 4499 JHparent145 GNWFDP 4606 JHparent146 HEYFQH 4493JHparent147 HNWFDP 4598 JHparent148 HWYFDL 4531 JHparent149 IEYFQH 4497JHparent150 INWFDP 4604 JHparent151 LEYFQH 4495 JHparent152 LNWFDP 4602JHparent153 NEYFQH 4496 JHparent154 NNWFDP 4634 JHparent155 NWYFDL 4532JHparent156 PEYFQH 4491 JHparent157 PNWFDP 4603 JHparent158 REYFQH 4494JHparent159 RNWFDP 4601 JHparent160 SEYFQH 4490 JHparent161 SNWFDP 4600JHparent162 TEYFQH 4487 JHparent163 TNWFDP 4605 JHparent164 VEYFQH 4500JHparent165 VNWFDP 4607 JHparent166 YEYFQH 4501 JHparent167 YNWFDP 4597JHparent168 YWYFDL 4527 JHparent169 AYYYGMDV 4679 JHparent170 AYYYYMDV4725 JHparent171 DYYYGMDV 4668 JHparent172 DYYYYMDV 4714 JHparent173FYYYGMDV 4670 JHparent174 FYYYYMDV 4716 JHparent175 GYYYGMDV 4677JHparent176 GYYYYMDV 4723 JHparent177 HYYYGMDV 4669 JHparent178 HYYYYMDV4715 JHparent179 IYYYGMDV 4675 JHparent180 IYYYYMDV 4721 JHparent181LYYYGMDV 4673 JHparent182 LYYYYMDV 4719 JHparent183 NYYYGMDV 4667JHparent184 NYYYYMDV 4713 JHparent185 PYYYGMDV 4674 JHparent186 PYYYYMDV4720 JHparent187 RYYYGMDV 4672 JHparent188 RYYYYMDV 4718 JHparent189SYYYGMDV 4671 JHparent190 SYYYYMDV 4717 JHparent191 TYYYGMDV 4676JHparent192 TYYYYMDV 4722 JHparent193 VYYYGMDV 4678 JHparent194 VYYYYMDV4724 JHparent195 AYYYYGMDV 4666 JHparent196 AYYYYYMDV 4712 JHparent197DYYYYGMDV 4655 JHparent198 DYYYYYMDV 4701 JHparent199 FYYYYGMDV 4657JHparent200 FYYYYYMDV 4703 JHparent201 GYYYYGMDV 4664 JHparent202GYYYYYMDV 4710 JHparent203 HYYYYGMDV 4656 JHparent204 HYYYYYMDV 4702JHparent205 IYYYYGMDV 4662 JHparent206 IYYYYYMDV 4708 JHparent207LYYYYGMDV 4660 JHparent208 LYYYYYMDV 4706 JHparent209 NYYYYGMDV 4654JHparent210 NYYYYYMDV 4700 JHparent211 PYYYYGMDV 4661 JHparent212PYYYYYMDV 4707 JHparent213 RYYYYGMDV 4659 JHparent214 RYYYYYMDV 4705JHparent215 SYYYYGMDV 4658 JHparent216 SYYYYYMDV 4704 JHparent217TYYYYGMDV 4663 JHparent218 TYYYYYMDV 4709 JHparent219 VYYYYGMDV 4665JHparent220 VYYYYYMDV 4711 JHparent221 AYYYYYGMDV 4653 JHparent222AYYYYYYMDV 4699 JHparent223 DYYYYYGMDV 4635 JHparent224 DYYYYYYMDV 4681JHparent225 FYYYYYGMDV 4644 JHparent226 FYYYYYYMDV 4690 JHparent227GYYYYYGMDV 4651 JHparent228 GYYYYYYMDV 4697 JHparent229 HYYYYYGMDV 4643JHparent230 HYYYYYYMDV 4689 JHparent231 IYYYYYGMDV 4649 JHparent232IYYYYYYMDV 4695 JHparent233 LYYYYYGMDV 4647 JHparent234 LYYYYYYMDV 4693JHparent235 NYYYYYGMDV 4680 JHparent236 NYYYYYYMDV 4726 JHparent237PYYYYYGMDV 4648 JHparent238 PYYYYYYMDV 4694 JHparent239 RYYYYYGMDV 4646JHparent240 RYYYYYYMDV 4692 JHparent241 SYYYYYGMDV 4645 JHparent242SYYYYYYMDV 4691 JHparent243 TYYYYYGMDV 4650 JHparent244 TYYYYYYMDV 4696JHparent245 VYYYYYGMDV 4652 JHparent246 VYYYYYYMDV 4698 JHparent247YYYYYYGMDV 4642 JHparent248 YYYYYYYMDV 4688

TABLE 16 Polynucleotide sequences of 27 human IGHD genes and alleles.IGHD Gene Polynucleotide Sequence SEQ ID NO IGHD1-(1)-01GGTACAACTGGAACGAC 4739 IGHD1-20 GGTATAACTGGAACGAC 4740 IGHD1-26GGTATAGTGGGAGCTACTAC 4741 IGHD1-7 GGTATAACTGGAACTAC 4742 IGHD2-15-01AGGATATTGTAGTGGTGGTAGCTGCTACTCC 4743 IGHD2-2-xAGGATATTGTAGTAGTACCAGCTGCTATGCC 4744 IGHD2-2-yAGGATATTGTAGTAGTACCAGCTGCTATACC 4745 IGHD2-2-zTGGATATTGTAGTAGTACCAGCTGCTATGCC 4746 IGHD2-21-01AGCATATTGTGGTGGTGATTGCTATTCC 4747 IGHD2-21-02AGCATATTGTGGTGGTGACTGCTATTCC 4748 IGHD2-8-01AGGATATTGTACTAATGGTGTATGCTATACC 4749 IGHD3-10-01GTATTACTATGGTTCGGGGAGTTATTATAAC 4750 IGHD3-10-03GTATTACTATGGTTCAGGGAGTTATTATAAC 4751 IGHD3-16-02GTATTATGATTACGTTTGGGGGAGTTATGCTTATACC 4752 IGHD3-22-01GTATTACTATGATAGTAGTGGTTATTACTAC 4753 IGHD3-3-01GTATTACGATTTTTGGAGTGGTTATTATACC 4754 IGHD3-9-01GTATTACGATATTTTGACTGGTTATTATAAC 4755 IGHD4-17 TGACTACGGTGACTAC 4756IGHD4-23-01 TGACTACGGTGGTAACTCC 4757 IGHD4-4/11- TGACTACAGTAACTAC 475801 IGHD5-12-01 GTGGATATAGTGGCTACGATTAC 4759 IGHD5-24-01GTAGAGATGGCTACAATTAC 4760 IGHD5-5/18- GTGGATACAGCTATGGTTAC 4761 01IGHD6-13-01 GGGTATAGCAGCAGCTGGTAC 4762 IGHD6-19-01 GGGTATAGCAGTGGCTGGTAC4763 IGHD6-6-01 GAGTATAGCAGCTCGTCC 4764 IGHD7-27-01 CTAACTGGGGA 4765

TABLE 17 Theoretical segment pool of 73 DH parent Segments. “Z”represents a stop codon. DH Parent Segment Amino Acid Name SequenceSEQ ID NO DHparent001 LTG n/a DHparent002 NWG n/a DHparent003 ZLG n/aDHparent004 DYGDY 4348 DHparent005 DYSNY 4366 DHparent006 TTVTT 4351DHparent007 VQLER 3737 DHparent008 VZLEL 4766 DHparent009 VZLER 4767DHparent010 VZQLV 4768 DHparent011 YNWND 3740 DHparent012 YNWNY 3765DHparent013 ZLQZL 4769 DHparent014 ZLRZL 4770 DHparent015 DYGGNS 4354DHparent016 EYSSSS 4476 DHparent017 GITGTT 3743 DHparent018 GTTGTT 3731DHparent019 GYSYGY 4429 DHparent020 RDGYNY 4407 DHparent021 SIAARP 4481DHparent022 TTVVTP 4360 DHparent023 VZQQLV 4771 DHparent024 VZQWLV 4772DHparent025 VZWELL 4773 DHparent026 WIQLWL 4423 DHparent027 YSGSYY 3759DHparent028 ZLRWZL 4774 DHparent029 ZRWLQL 4775 DHparent030 GIAAAGT 4445DHparent031 GIAVAGT 4465 DHparent032 GIVGATT 3748 DHparent033 GYSGYDY4385 DHparent034 GYSSGWY 4456 DHparent035 GYSSSWY 4435 DHparent036VDTAMVT 4413 DHparent037 VEMATIT 4395 DHparent038 WIZWLRL 4776DHparent039 VDIVATIT 4369 DHparent040 AYCGGDCYS 3862 DHparent041HIVVVIAIP 3871 DHparent042 HIVVVTAIP 3891 DHparent043 SILWWZLLF 4777DHparent044 DIVLMVYAIP 3920 DHparent045 DIVVVPAAIP 3846 DHparent046DIVVVPAAMP 3816 DHparent047 DIVVVVAATP 3777 DHparent048 GYCSGGSCYS 3767DHparent049 GYCSSTSCYA 3806 DHparent050 GYCSSTSCYT 3843 DHparent051GYCTNGVCYT 3910 DHparent052 ITIFGVVIIP 4269 DHparent053 ITIFZLVIIT4778 & 8746 DHparent054 ITMIVVVITT 4189 DHparent055 ITMVQGVIIT 4040DHparent056 ITMVRGVIIT 3994 DHparent057 RILYZWCMLY 4779 & 8747DHparent058 RILZWWZLLL 4780 DHparent059 RILZZYQLLC 4781 DHparent060RILZZYQLLY 4782 DHparent061 VLLWFGELLZ 4783 DHparent062 VLLWFRELLZ 4784DHparent063 VLLZZZWLLL 4785 DHparent064 VLRFLEWLLY 4214 DHparent065VLRYFDWLLZ 4786 DHparent066 WILZZYQLLC 4787 DHparent067 YYDFWSGYYT 4242DHparent068 YYDILTGYYN 4318 DHparent069 YYYDSSGYYY 4161 DHparent070YYYGSGSYYN 3969 DHparent071 IMITFGGVMLIP 4115 DHparent072 VLZLRLGELCLY4788 DHparent073 YYDYVWGSYAYT 4070

TABLE 18 Application of Equation 1 to Test Case 1. Type Segment WeightTN1 R 1.0 DH TA 1.0 N2 H 1.0 H3-JH HFDY (SEQ ID NO: 4582) 1.0

TABLE 19 Application of Equation 1 to Test Cases 2.1 and 2.1. TypeSegment Weight TN1 V and VG 0.5 each DH GIVGA (SEQ ID NO: 3751) and 0.5each IVGA (SEQ ID NO: 3755) N2 AS 1.0 H3-JH Y 1.0

TABLE 20 Application of Equation 1 to Test Case 3.1. Type Segment WeightTN1 DR 1.0 DH YSGYD (SEQ ID NO: 4389) 0.8 N2 LG 1.0 H3-JH Y 1.0

TABLE 21 Application of Equation 1 to Test Cases 4.1 and 4.2. TypeSegment Weight TN1 “—” and G 0.5 each DH GIAAA (SEQ ID NO: 4448) and 0.5each IAAA (SEQ ID NO: 4452) N2 D 1.0 H3-JH SNWFDP (SEQ ID NO: 4600) 0.83

TABLE 22 Application of Equation 1 to all Test Cases. Type SegmentsWeight TN1 DR, R, VG, V, G and “—” 0.25, 0.25, 0.125, 0.125, 0.125 and0.125 respectively DH TA, YSGYD (SEQ ID NO: 4389), 0.25, 0.20, 0.125,IAAA (SEQ ID NO: 4452), GIAAA (SEQ ID NO: 4448), 0.125, 0.125 and 0.125IVGA (SEQ ID NO: 3755) and GIVGA (SEQ ID NO: 3751) respectively N2 AS,H, D and LG 0.25 each H3-JH Y, HFDY and SNWFDP 0.50, 0.25 and 0.209

TABLE 23 Segments used in Exemplary Library Design 1 (ELD-1).The sequences collectively form a theoretical segmentpool that comprises individual theoretical segmentpools of TN1, DH, N2, and H3-JH segments. Segment SEQ SEQ SEQ No. TN1ID NO DH ID NO N2 H3-JH ID NO 1 — n/a YYYDSSGYY 4162 — DAFDI 4538 2 Gn/a YGDY 4350 Y YYFDY 4568 3 D n/a DYGDY 4348 G Y n/a 4 A n/a YYYDSSGY4164 D FDY n/a 5 V n/a YCSSTSCY 3810 S DY n/a 6 DR n/a YYDSSGY 4168 PYGMDV 4640 7 S n/a YCSGGSCY 3771 F — n/a 8 L n/a YCSSTSC 3812 L LDY n/a9 DL n/a GG n/a A GAFDI 4549 10 R n/a RG n/a E YFDY 4567 11 GR n/a SGSY3763 V YYGMDV 4639 12 T n/a YYDSSGYY 4165 H AFDI 4539 13 GG n/a SS n/a TPFDY 4587 14 E n/a YDFWSGY 4249 R GMDV 4641 15 DS n/a GYCSSTSC 3809 WGWFDP 4619 16 VG n/a DY n/a SG YYYYGMDV 4637 17 DG n/a YYYDSSG 4167 IIDY n/a 18 AP n/a CSSTSCY 3813 RG GYFDY 4577 19 GL n/a YYDFWSGY 4245 KGFDY 4590 20 GS n/a SSGWY 4461 LG MDV n/a 21 DRG n/a AG n/a Q VDY n/a 22DLG n/a DSSGY 4179 GP YYYGMDV 4638 23 VP n/a SSSW 4443 PG NWFDP 4595 24DP n/a VGAT 3756 LP PDY n/a 25 P n/a SY n/a AG WFDP 4596 26 EG n/a DTAM4420 GS NFDY 4580 27 GA n/a IAAAG 4449 TS YWYFDL 4527 28 AG n/a YSSSW4439 SS NAFDI 4565 29 GV n/a GS n/a GG HFDY 4582 30 GP n/a YYDSSG 4172YS SFDY 4584 31 ER n/a VG n/a M YYYYYGMDV 4636 32 DV n/a YSSSWY 4437 SLDYYYGMDV 4668 33 VGG n/a YCSGGSC 3773 SP DFDY 4581 34 SG n/a YDSSGYY4169 SD YNWFDP 4597 35 GRG n/a GI n/a AP DYYYYGMDV 4655 36 DA n/aGYCSGGSCY 3768 GR YYYMDV 4685 37 DRP n/a YSSS 4442 TG LFDY 4586 38 DSGn/a SSGW 4463 SR DYFDY 4566 39 GPR n/a TA n/a LD NYYYYGMDV 4654 40 DTn/a DSSGYY 4174 LS GDY n/a 41 GGG n/a GYCSSTSCY 3807 GA YDY n/a 42 DRGG3720 TTVT 4352 VG SYFDY 4571 43 PL n/a YSSGWY 4458 PP YYMDV 4686 44 DPSn/a GW n/a RR TFDY 4589 45 LP n/a LG n/a GSG YYYYYYGMDV 4642 46 RG n/aDYGD 4349 GT FFDY 4583 47 GT n/a TVTT 4353 TP SYYYYGMDV 4658 48 LG n/aAAA n/a RP VFDY 4591 49 DLP n/a YSSGW 4460 RD YAFDI 4540 50 DGR n/a LVn/a QL SDY n/a 51 ERG n/a YYDFWSGYY 4243 TT WYFDL 4528 52 DGS n/aYYDSSGYYY 4163 PL DWFDP 4609 53 ES n/a YYDFWSG 4248 RS AFDY 4592 54 PSn/a YGD n/a WS PYYYYGMDV 4661 55 GGS n/a YG n/a RV HYFDY 4569 56 DPR n/aGT n/a RF DV n/a 57 EA n/a YSGSY 3760 SF RFDY 4585 58 GGR n/a YYYGSGSY3972 PT NYFDY 4593 59 DGG n/a LR n/a PS IFDY 4588 60 SP n/a SSS n/a ATADY n/a 61 DPG n/a GD n/a RL HYYYGMDV 4669 62 DSGG 3723 CSGGSCY 3774 SVGYYYYYGMDV 4651 63 DPL n/a GY n/a RA DWYFDL 4530 64 TP n/a YCGGDCY 3866GRG GYYYGMDV 4677 65 AGG n/a QWLV 4475 VP YYYYMDV 4684 66 PR n/a IAAA4452 SQ V n/a 67 DGT n/a QG n/a AS FDP n/a 68 GLG n/a YCSSTSCYT 3844 PRDDY n/a 69 DSP n/a SG n/a VD GYYYYGMDV 4664 70 GGV n/a TTVTT 4351 GVYMDV 4687 71 GPS n/a QQL n/a LT NWYFDL 4532 72 GVG n/a IAVA 4472 TRPYFDY 4574 73 GGA n/a GDY n/a VS FDI n/a 74 RP n/a YYYDSSGYYY 4161 SGLNDY n/a 75 DPP n/a DGYN 4411 RGG HYYYYYGMDV 4643 76 DPT n/a CSSTSC 3814LR VYYYGMDV 4678 77 EV n/a IVVVPAAI 3849 SGG VYFDY 4578 78 GAP n/a AVAG4473 SA SNWFDP 4600 79 DGRG 3702 AA n/a AY NYYYGMDV 4667 80 GPP n/a DSSG4185 LE LYYYYGMDV 4660 81 DLGG 3714 VR n/a TGG DYYYYYGMDV 4635 82 ET n/aYDFWSG 4253 GL AAFDI 4551 83 DGGP 3681 GYSSSWY 4435 GAG SWFDP 4613 84DGGR 3684 SGW n/a TA SYYYYYGMDV 4645 85 DGSG 3705 WG n/a IS SYYYGMDV4671 86 GTG n/a SGSYY 3761 ST P n/a 87 DPGG 3717 RY n/a SSE YYYYYMDV4683 88 DGGS 3687 DS n/a GLG RAFDI 4544 89 GGT n/a TT n/a PRP RYFDY 457290 GGRG 3703 YCSGGSCYS 3769 RPL FYYYYGMDV 4657 91 EGR n/a PA n/a EAGNWFDP 4606 92 DGA n/a IVVVPAA 3823 LGG RWFDP 4614 93 DGL n/a IAAAGT4447 FS GFDP 4623 94 EL n/a AAAG 4453 DL DYYYYMDV 4714 95 EGG n/aGYCSGGSC 3770 GGG PYYYGMDV 4674 96 LGG n/a SGWY 4464 AR DP n/a 97 DPAn/a DYGGN 4355 RE HAFDI 4541 98 EGV n/a YYYDS 4176 PA DNWFDP 4594 99 GSGn/a YSSG 4462 PTQ LNWFDP 4602 100 GGP n/a YDSSGY 4173 AGG AYYYYGMDV 4666101 VAG n/a GGV 102 SSW n/a SI 103 GSGSY 3987 SAA 104 SSSS 4480 GGS 105NW n/a GGA 106 DFWSGY 4254 GPG 107 QQLV 4455 PGG 108 YGGN 4358 AAE 109YDSSG 4178 GGR 110 GYSYG 4430 TW 111 TV n/a GGP 112 NG n/a GGL 113 IVGAT3752 VGG 114 IVGA 3755 GTG 115 YGSGSY 3981 NR 116 SSWY 4444 NTP 117 STn/a PV 118 DFWSGYY 4250 EK 119 GSY n/a GVG 120 YYDSS 4177 KV 121 VGA n/aEKR 122 AT n/a QT 123 RP n/a SE 124 YYYGSGS 3975 SAL 125 GIAAAG 4446 FH126 SGY n/a RH 127 TG n/a PTA 128 LT n/a HL 129 RD n/a WGT 130 WEL n/aREL 131 YSYG 4433 NT 132 TVT n/a HW 133 GYCSGGSCYS 3767 PKS 134 AR n/aDT 135 YYGSGSY 3976 DTL 136 RW n/a SK 137 DIVVVPA 3822 TGL 138 YSGS 3762AYH 139 GYSSSW 4436 ISR 140 YSSSS 4478 GGT 141 YYYDSS 4171 QE 142 QL n/a143 GYSGYD 4386 144 GE n/a 145 MA n/a 146 DSS n/a 147 RF n/a 148 DTAMV4417 149 YYGSGSYY 3973 150 VDTAMV 4414 151 FGVV 4293 152 EYSSS 4477 153TTV n/a 154 SWY n/a 155 IAARP 4483 156 VE n/a 157 SIAA 4484 158 YSGYD4389 159 DIVVVPAA 3819 160 CSGGSC 3775 161 DW n/a 162 TS n/a 163 RL n/a164 YSS n/a 165 GN n/a 166 SN n/a 167 GYSY 4432 168 YYDS 4183 169 VDTAM4416 170 LE n/a 171 AVAGT 4470 172 YSY n/a 173 SW n/a 174 SSG n/a 175FGV n/a 176 VP n/a 177 VA n/a 178 SYY n/a 179 QWL n/a 180 GSG n/a 181TIFGVV 4280 182 AVA n/a 183 FWSGY 4260 184 YSGSYY 3759 185 IAVAG 4469186 YS n/a 187 YQL n/a 188 SIAAR 4482 189 YCGGDC 3868 190 NWNY 3766 191SSSWY 4440 192 GIAVA 4468 193 YSYGY 4431 194 GIAAA 4448 195 YYG n/a 196AAG n/a 197 AV n/a 198 AYCGGDCY 3863 199 YYGSGS 3980 200 EY n/a

TABLE 24 Segments used in Exemplary Library Design 2 (ELD-2).The sequences collectively form a theoretical segmentpool that comprises individual theoretical segment poolsof TN1, DH, N2, and H3-JH segments. Segment SEQ SEQ SEQ SEQ No. TN1ID NO DH ID NO N2 ID NO H3-JH ID NO 1 — n/a YYYDSSGYY 4162 — n/a DAFDI4538 2 G n/a YGDY 4350 G n/a YYFDY 4568 3 D n/a DYGDY 4348 D n/a Y n/a 4A n/a YYYDSSGY 4164 A n/a FDY n/a 5 V n/a YCSSTSCY 3810 V n/a DY n/a 6 Sn/a YYDSSGY 4168 S n/a YGMDV 4640 7 DR n/a YCSGGSCY 3771 DR n/a — n/a 8L n/a YCSSTSC 3812 L n/a LDY n/a 9 R n/a GG n/a R n/a GAFDI 4549 10 DLn/a RG n/a DL n/a YFDY 4567 11 T n/a SGSY 3763 T n/a YYGMDV 4639 12 En/a YYDSSGYY 4165 E n/a AFDI 4539 13 GR n/a SS n/a GR n/a PFDY 4587 14GG n/a YDFWSGY 4249 GG n/a GMDV 4641 15 DG n/a GYCSSTSC 3809 DG n/aGWFDP 4619 16 DS n/a DY n/a DS n/a YYYYGMDV 4637 17 VG n/a YYYDSSG 4167VG n/a IDY n/a 18 EG n/a CSSTSCY 3813 EG n/a GYFDY 4577 19 P n/aYYDFWSGY 4245 P n/a GFDY 4590 20 GL n/a SSGWY 4461 GL n/a MDV n/a 21 GSn/a AG n/a GS n/a VDY n/a 22 DP n/a DSSGY 4179 DP n/a YYYGMDV 4638 23 GPn/a SSSW 4443 GP n/a NWFDP 4595 24 GA n/a VGAT 3756 GA n/a PDY n/a 25 GVn/a SY n/a GV n/a WFDP 4596 26 H n/a DTAM 4420 H n/a NFDY 4580 27 DRGn/a IAAAG 4449 DRG n/a YWYFDL 4527 28 DQ n/a YSSSW 4439 DQ n/a NAFDI4565 29 AG n/a GS n/a AG n/a HFDY 4582 30 DLG n/a YYDSSG 4172 DLG n/aSFDY 4584 31 DV n/a VG n/a DV n/a YYYYYGMDV 4636 32 Q n/a YSSSWY 4437 Qn/a DYYYGMDV 4668 33 N n/a YCSGGSC 3773 N n/a DFDY 4581 34 AP n/aYDSSGYY 4169 AP n/a YNWFDP 4597 35 GGG n/a GI n/a GGG n/a DYYYYGMDV 465536 DH n/a GYCSGGSCY 3768 DH n/a YYYMDV 4685 37 VP n/a YSSS 4442 VP n/aLFDY 4586 38 SG n/a SSGW 4463 SG n/a DYFDY 4566 39 GRG n/a TA n/a GRGn/a NYYYYGMDV 4654 40 AR n/a DSSGYY 4174 AR n/a GDY n/a 41 RG n/aGYCSSTSCY 3807 RG n/a YDY n/a 42 ER n/a TTVT 4352 ER n/a SYFDY 4571 43DA n/a YSSGWY 4458 DA n/a YYMDV 4686 44 AS n/a GW n/a AS n/a TFDY 458945 PL n/a LG n/a PL n/a YYYYYYGMDV 4642 46 DQG n/a DYGD 4349 DQG n/aFFDY 4583 47 VL n/a TVTT 4353 VL n/a SYYYYGMDV 4658 48 GT n/a AAA n/a GTn/a VFDY 4591 49 DGG n/a YSSGW 4460 DGG n/a YAFDI 4540 50 DSG n/a LV n/aDSG n/a SDY n/a 51 VGG n/a YYDFWSGYY 4243 VGG n/a WYFDL 4528 52 F n/aYYDSSGYYY 4163 F n/a DWFDP 4609 53 AL n/a YYDFWSG 4248 AL n/a AFDY 459254 PS n/a YGD n/a PS n/a PYYYYGMDV 4661 55 ES n/a YG n/a ES n/a HYFDY4569 56 ERG n/a GT n/a ERG n/a DV n/a 57 GGV n/a YSGSY 3760 GGV n/a RFDY4585 58 DRP n/a YYYGSGSY 3972 DRP n/a NYFDY 4593 59 EA n/a LR n/a EA n/aIFDY 4588 60 TP n/a SSS n/a TP n/a ADY n/a 61 GPR n/a GD n/a GPR n/aHYYYGMDV 4669 62 LH n/a CSGGSCY 3774 LH n/a GYYYYYGMDV 4651 63 SR n/a GYn/a SR n/a DWYFDL 4530 64 LP n/a YCGGDCY 3866 LP n/a GYYYGMDV 4677 65 LGn/a QWLV 4475 LG n/a YYYYMDV 4684 66 DT n/a IAAA 4452 DT n/a V n/a 67 VAn/a QG n/a VA n/a FDP n/a 68 SL n/a YCSSTSCYT 3844 SL n/a DDY n/a 69 EGGn/a SG n/a EGG n/a GYYYYGMDV 4664 70 DRS n/a TTVTT 4351 DRS n/a YMDV4687 71 K n/a QQL n/a K n/a NWYFDL 4532 72 DPG n/a IAVA 4472 DPG n/aPYFDY 4574 73 I n/a GDY n/a I n/a FDI n/a 74 GD n/a YYYDSSGYYY 4161 GDn/a NDY n/a 75 DGT n/a DGYN 4411 DGT n/a HYYYYYGMDV 4643 76 GPP n/aCSSTSC 3814 GPP n/a VYYYGMDV 4678 77 DPP n/a IVVVPAAI 3849 DPP n/a VYFDY4578 78 RR n/a AVAG 4473 RR n/a SNWFDP 4600 79 EGV n/a AA n/a EGV n/aNYYYGMDV 4667 80 GF n/a DSSG 4185 GF n/a LYYYYGMDV 4660 81 GVG n/a VRn/a GVG n/a DYYYYYGMDV 4635 82 DPS n/a YDFWSG 4253 DPS n/a AAFDI 4551 83VD n/a GYSSSWY 4435 VD n/a SWFDP 4613 84 GGT n/a SGW n/a GGT n/aSYYYYYGMDV 4645 85 DK n/a WG n/a DK n/a SYYYGMDV 4671 86 GTG n/a SGSYY3761 GTG n/a P n/a 87 DF n/a RY n/a DF n/a YYYYYMDV 4683 88 GQ n/a DSn/a GQ n/a RAFDI 4544 89 SP n/a TT n/a SP n/a RYFDY 4572 90 QG n/aYCSGGSCYS 3769 QG n/a FYYYYGMDV 4657 91 DLT n/a PA n/a DLT n/a GNWFDP4606 92 AK n/a IVVVPAA 3823 AK n/a RWFDP 4614 93 GPS n/a IAAAGT 4447 GPSn/a GFDP 4623 94 QR n/a AAAG 4453 QR n/a DYYYYMDV 4714 95 VR n/aGYCSGGSC 3770 VR n/a PYYYGMDV 4674 96 DSP n/a SGWY 4464 DSP n/a DP n/a97 DPL n/a DYGGN 4355 DPL n/a HAFDI 4541 98 EGR n/a YYYDS 4176 EGR n/aDNWFDP 4594 99 GRRG 4789 YSSG 4462 GRRG 4789 LNWFDP 4602 100 EV n/aYDSSGY 4173 EV n/a AYYYYGMDV 4666 101 VAG n/a RP n/a 102 SSW n/a GH n/a103 GSGSY 3987 DGR n/a 104 SSSS 4480 AA n/a 105 NW n/a DD n/a 106 DFWSGY4254 W n/a 107 QQLV 4455 GGS n/a 108 YGGN 4358 DIS n/a 109 YDSSG 4178GGA n/a 110 GYSYG 4430 GK n/a 111 TV n/a DGP n/a 112 NG n/a DLK n/a 113IVGAT 3752 ET n/a 114 IVGA 3755 TT n/a 115 YGSGSY 3981 VH n/a 116 SSWY4444 AE n/a 117 ST n/a VS n/a 118 DFWSGYY 4250 LGG n/a 119 GSY n/a C n/a120 YYDSS 4177 DKG n/a 121 VGA n/a HA n/a 122 AT n/a VI n/a 123 RP n/aHP n/a 124 YYYGSGS 3975 GGE n/a 125 GIAAAG 4446 EP n/a 126 SGY n/a EFn/a 127 TG n/a DRN n/a 128 LT n/a DWG n/a 129 RD n/a GE n/a 130 WEL n/aDRA n/a 131 YSYG 4433 VN n/a 132 TVT n/a DRE n/a 133 GYCSGGSCYS 3767 DLAn/a 134 AR n/a EN n/a 135 YYGSGSY 3976 VT n/a 136 RW n/a HG n/a 137DIVVVPA 3822 RA n/a 138 YSGS 3762 M n/a 139 GYSSSW 4436 DVP n/a 140YSSSS 4478 GAP n/a 141 YYYDSS 4171 GLG n/a 142 QL n/a GPG n/a 143 GYSGYD4386 PG n/a 144 GE n/a DSS n/a 145 MA n/a SS n/a 146 DSS n/a AGG n/a 147RF n/a GGR n/a 148 DTAMV 4417 GPN n/a 149 YYGSGSYY 3973 DRL n/a 150VDTAMV 4414 GRR n/a 151 FGVV 4293 DSGG 3723 152 EYSSS 4477 TR n/a 153TTV n/a DLS n/a 154 SWY n/a RGG n/a 155 IAARP 4483 Y n/a 156 VE n/a EVRn/a 157 SIAA 4484 LI n/a 158 YSGYD 4389 TF n/a 159 DIVVVPAA 3819 LK n/a160 CSGGSC 3775 DLE n/a 161 DW n/a GY n/a 162 TS n/a DGS n/a 163 RL n/aGVR n/a 164 YSS n/a GQR n/a 165 GN n/a EGL n/a 166 SN n/a VLG n/a 167GYSY 4432 QP n/a 168 YYDS 4183 VM n/a 169 VDTAM 4416 VE n/a 170 LE n/aDQGG 4790 171 AVAGT 4470 PN n/a 172 YSY n/a DGL n/a 173 SW n/a PV n/a174 SSG n/a HR n/a 175 FGV n/a AD n/a 176 VP n/a DLF n/a 177 VA n/a LDn/a 178 SYY n/a GGD n/a 179 QWL n/a DRR n/a 180 GSG n/a DHH n/a 181TIFGVV 4280 DW n/a 182 AVA n/a DAS n/a 183 FWSGY 4260 GW n/a 184 YSGSYY3759 SV n/a 185 IAVAG 4469 GLR n/a 186 YS n/a DGA n/a 187 YQL n/a LA n/a188 SIAAR 4482 EEG n/a 189 YCGGDC 3868 AV n/a 190 NWNY 3766 VQ n/a 191SSSWY 4440 AH n/a 192 GIAVA 4468 RS n/a 193 YSYGY 4431 WA n/a 194 GIAAA4448 LR n/a 195 YYG n/a GSG n/a 196 AAG n/a GGSG 3706 197 AV n/a DLR n/a198 AYCGGDCY 3863 VWG n/a 199 YYGSGS 3980 HL n/a 200 EY n/a EH n/a

TABLE 25 Segments used in Exemplary Library Design 3 (ELD-3). Thesequences collectively form a theoretical segment pool that comprisesindividual theoretical segment pools of TN1, DH, N2, and H3-JH segments.TN1 Seg- (plus SEQ TN1 Nucleotides SEQ SEQ SEQ SEQ SEQ ment AR or ID(plus AR or ID ID ID N2 ID ID No. AK)¹ NO AK)¹ NO DH NO DH NucleotidesNO N2 Nucleotides H3-JH NO H3-JH Nucleotides NO 1 AR n/a GCCAGA n/a GTn/a GGTACT n/a — — — n/a — n/a 2 ARE n/a GCCAGAGAG n/a TT n/a ACTACT n/aA GCT Y n/a TAT n/a 3 ARD n/a GCCAGAGAC n/a TG n/a ACAGGC n/a D GAT DIn/a GATATT n/a 4 ARG n/a GCCAGAGGA n/a ER n/a GAGCGT n/a E GAG DL n/aGACTTG n/a 5 AREG 4791 GCCAGAGAGGGA 4963 QLE n/a CAATTAGAG n/a F TTC DPn/a GATCCT n/a 6 ARDG 4792 GCCAGAGACGGA 4964 LER n/a TTAGAGCGT n/a G GGCDV n/a GATGTA n/a 7 ARGG 4793 GCCAGAGGTGGA 4965 VGAT 3756 GTTGGCGCAACT5135 H CAT DY n/a GACTAT n/a 8 ARR n/a GCCAGGAGA n/a YSG n/a TATAGTGGTn/a I ATC QH n/a CAGCAC n/a 9 ARER 4794 GCCAGAGAGAGA 4966 YSGSY 3760TACTCTGGCTCTTAT 5136 K AAA ADY n/a GCTGATTAT n/a 10 ARDR 4795GCCAGAGACAGA 4967 VG n/a GTAGGC n/a L CTG DDY n/a GATGACTAT n/a 11 ARGR4796 GCCAGAGGCAGA 4968 AT n/a GCCACT n/a M ATG FDI n/a TTTGACATT n/a 12ARS n/a GCCAGATCT n/a WEL n/a TGGGAGCTT n/a P CCT FDL n/a TTCGACTTA n/a13 ARES 4797 GCCAGAGAATCT 4969 YS n/a TACAGC n/a Q CAG FDP n/a TTTGACCCTn/a 14 ARDS 4798 GCCAGAGACTCT 4970 SG n/a AGTGGT n/a R AGG FDY n/aTTCGACTAT n/a 15 ARGS 4799 GCCAGAGGTTCT 4971 GS n/a GGTTCT n/a S TCA FQHn/a TTCCAGCAC n/a 16 ARP n/a GCCAGACCT n/a SY n/a AGCTAC n/a T ACC GDYn/a GGTGACTAC n/a 17 ARDP 4800 GCCAGAGACCCT 4972 CSSTSC 3814TGTAGTAGTACAAGTTGC 5137 V GTT IDY n/a ATCGACTAT n/a 18 ARGP 4801GCCAGAGGGCCT 4973 CSSTSCY 3813 TGCTCATCTACATCATGCTAT 5138 W TGG LDY n/aTTGGACTAT n/a 19 ARL n/a GCCAGATTG n/a YCSSTSC 3812TATTGTTCAAGTACATCTTGT 5139 Y TAC MDV n/a ATGGATGTG n/a 20 ARDL 4802GCCAGAGACTTG 4974 GYCSSTSC 3809 GGGTATTGCTCCAGTACCTCATGT 5140 AD GCTGATPDY n/a CCAGATTAT n/a 21 ARGL 4803 GCCAGAGGGTTG 4975 YCSSTSCY 3810TACTGCAGCAGCACAAGTTGTTAC 5141 AG GCAGGC SDY n/a TCTGATTAC n/a 22 ARA n/aGCCAGAGCT n/a GYCSSTSCY 3807 GGGTATTGCAGTTCAACTAGTTGTTAT 5142 AP GCCCCAVDY n/a GTTGACTAC n/a 23 AREA 4804 GCCAGAGAGGCT 4976 YCSSTSCYT 3844TACTGTTCATCAACCTCCTGTTATACT 5143 AQ GCTCAG YDY n/a TATGATTAC n/a 24 ARDA4805 GCCAGAGATGCT 4977 PAA n/a CCTGCCGCT n/a AR GCTAGG AFDI 4539GCCTTCGATATC 5251 25 ARGA 4806 GCCAGAGGTGCT 4978 CSGGSCY 3774TGCTCTGGGGGTAGCTGCTAT 5144 AS GCTAGT AFDY 4592 GCCTTCGATTAC 5252 26 ARTn/a GCCAGAACT n/a YCSGGSC 3773 TACTGTAGCGGTGGTAGTTGC 5145 AT GCTACC DFDY4581 GATTTCGACTAT 5253 27 ARET 4807 GCCAGAGAGACT 4979 GYCSGGSC 3770GGATACTGTAGTGGCGGATCCTGC 5146 AY GCCTAC FFDY 4583 TTCTTCGATTAC 5254 28ARDT 4808 GCCAGAGATACT 4980 YCSGGSCY 3771 TACTGCTCCGGAGGAAGTTGTTAT 5147DA GACGCC GFDP 4623 GGGTTTGACCCA 5255 29 ARGT 4809 GCCAGAGGCACT 4981GYCSGGSCY 3768 GGTTATTGCAGTGGGGGTTCATGTTAC 5148 DD GACGAT GFDY 4590GGGTTCGACTAC 5256 30 ARV n/a GCCAGAGTG n/a YCSGGSCYS 3769TACTGTTCCGGAGGTAGCTGTTACTCT 5149 DE GACGAG GMDV 4641 GGCATGGATGTA 525731 AREV 4810 GCCAGAGAGGTG 4982 RI n/a AGAATC n/a DG GATGGT HFDY 4582CACTTTGACTAT 5258 32 ARDV 4811 GCCAGAGATGTG 4983 GY n/a GGATAT n/a DLGACTTG IFDY 4588 ATATTCGATTAC 5259 33 ARGV 4812 GCCAGAGGGGTG 4984 GG n/aGGCGGT n/a DP GACCCT LFDY 4586 TTATTTGATTAT 5260 34 AREGG 4813GCCAGAGAGGGAGGA 4985 ATP n/a GCTACCCCT n/a DS GACTCC NFDY 4580AACTTTGATTAC 5261 35 ARDGG 4814 GCCAGAGATGGTGGA 4986 DI n/a GACATC n/aDY GATTAT PFDY 4587 CCCTTCGACTAT 5262 36 ARGGG 4815 GCCAGAGGTGGAGGA 4987TP n/a ACTCCT n/a EA GAGGCC RFDY 4585 AGGTTTGACTAT 5263 37 ARDGR 4816GCCAGAGACGGCAGA 4988 GD n/a GGAGAT n/a ED GAGGAC SFDY 4584 AGTTTCGATTAC5264 38 ARGGS 4817 GCCAGAGGCGGTTCT 4989 AYCGGDCY 3863GCCTATTGCGGTGGTGACTGCTAT 5150 EG GAAGGA TFDY 4589 ACATTTGACTAC 5265 39ARGGP 4818 GCCAGAGGTGGGCCT 4990 AYCGGDC 3865 GCATATTGCGGAGGGGATTGC 5151EK GAGAAA VFDY 4591 GTTTTCGATTAT 5266 40 ARGGA 4819 GCCAGAGGAGGTGCT 4991YCGGDCY 3866 TATTGTGGTGGGGACTGCTAT 5152 ER GAAAGA WFDP 4596 TGGTTCGATCCA5267 41 ARDGT 4820 GCCAGAGACGGTACT 4992 YCGGDC 3868 TACTGCGGAGGCGATTGC5153 ES GAATCT YFDL 4529 TATTTCGACTTA 5268 42 ARGGT 4821 GCCAGAGGTGGAACT4993 HI n/a CACATC n/a ET GAAACA YFDY 4567 TACTTCGATTAC 5269 43 AREGV4822 GCCAGAGAGGGAGTG 4994 TA n/a ACAGCT n/a FA TTCGCT YFQH 4489TATTTCCAGCAC 5270 44 ARGGV 4823 GCCAGAGGTGGCGTG 4995 GYCSSTSCYA 3806GGGTACTGCTCTAGCACTTCATGCTAC 5154 FH TTCCAT YMDV 4687 TATATGGATGTC 5271GCC 45 ARRG 4824 GCCAGAAGAGGA 4996 SS n/a AGTTCT n/a FL TTCTTG DAFDI4538 GATGCCTTCGACATA 5272 46 ARERG 4825 GCCAGAGAGCGTGGA 4997 ST n/aAGTACT n/a FR TTTAGG DWFDP 4609 GACTGGTTTGACCCC 5273 47 ARDRG 4826GCCAGAGATCGTGGA 4998 TS n/a ACCAGC n/a FS TTTAGT DYFDY 4566GACTACTTTGATTAC 5274 48 ARGRG 4827 GCCAGAGGCAGGGGA 4999 PAAMP 3835CCAGCAGCTATGCCT 5155 GA GGAGCC EYFQH 4488 GAATACTTCCAACAC 5275 49 ARSG4828 GCCAGATCAGGA 5000 PA n/a CCCGCC n/a GD GGTGAT GAFDI 4549GGCGCATTCGATATT 5276 50 ARDSG 4829 GCCAGAGACTCAGGA 5001 MP n/a ATGCCTn/a GE GGTGAG GWFDP 4619 GGGTGGTTTGATCCA 5277 51 ARDPG 4830GCCAGAGATCCAGGA 5002 VYAIP 3940 GTCTATGCAATTCCT 5156 GG GGAGGC GYFDY4577 GGCTATTTTGACTAC 5278 52 ARLG 4831 GCCAGATTGGGA 5003 WFGE 3966TGGTTTGGGGAG 5157 GL GGATTG HAFDI 4541 CATGCTTTTGATATA 5279 53 ARDLG4832 GCCAGAGACTTGGGA 5004 FGE n/a TTTGGAGAG n/a GP GGACCA HYFDY 4569CATTACTTCGATTAC 5280 54 ARAG 4833 GCCAGAGCTGGA 5005 GEL n/a GGCGAGCTTn/a GR GGTAGG NAFDI 4565 AACGCATTCGATATT 5281 55 ARVG 4834 GCCAGAGTGGGA5006 WFG n/a TGGTTCGGT n/a GS GGCAGT NWFDP 4595 AACTGGTTCGATCCA 5282 56ARGVG 4835 GCCAGAGGCGTAGGA 5007 GSG n/a GGTTCAGGC n/a GT GGAACA NYFDY4593 AATTATTTCGACTAT 5283 57 ARPR 4836 GCCAGACCCAGA 5008 SGSY 3763AGTGGATCTTAT 5158 GV GGAGTT PYFDY 4574 CCCTACTTTGACTAT 5284 58 ARGPR4837 GCCAGAGGACCAAGA 5009 YYGS 3990 TATTATGGCAGT 5159 GW GGATGG RAFDI4544 AGAGCCTTTGATATC 5285 59 ARPS 4838 GCCAGACCATCT 5010 YYYG 3989TACTACTATGGC 5160 GY GGATAT RYFDY 4572 AGGTACTTCGATTAC 5286 60 ARDPS4839 GCCAGAGATCCCTCT 5011 GSGSY 3987 GGCAGCGGTTCCTAC 5161 HE CATGAGSWFDP 4613 TCATGGTTCGACCCC 5287 61 ARGPS 4840 GCAAGAGGACCTTCT 5012 SGSYY3761 AGTGGATCCTATTAC 5162 HL CATTTG SYFDY 4571 AGTTACTTTGACTAT 5288 62ARDPP 4841 GCCAGAGACCCACCT 5013 YYYGSG 3979 TATTACTACGGGTCTGGC 5163 HPCATCCT TYFDY 4576 ACTTATTTCGACTAC 5289 63 ARGPP 4842 GCCAGAGGACCGCCT5014 SGS n/a AGCGGCAGT n/a HS CACTCC VAFDI 4550 GTGGCCTTCGACATT 5290 64ARPL 4843 GCCAGACCGTTG 5015 YYYGSGS 3975 TATTACTACGGATCTGGCTCT 5164 IFATCTTC VYFDY 4578 GTCTATTTTGATTAT 5291 65 ARDPL 4844 GCCAGAGATCCTTTG5016 YYYGSGSY 3972 TATTACTATGGCTCTGGTAGCTAC 5165 IG ATCGGC WYFDL 4528TGGTATTTCGATTTG 5292 66 ARRP 4845 GCCAGAAGGCCT 5017 YGS n/a TATGGCTCCn/a IR ATAAGG YAFDI 4540 TACGCATTTGACATC 5293 67 ARDRP 4846GCCAGAGACCGTCCT 5018 YYG n/a TACTATGGT n/a IS ATCAGT YGMDV 4640TACGGCATGGACGTG 5294 68 ARSP 4847 GCCAGATCACCT 5019 YYY n/a TATTATTATn/a KG AAAGGA YYFDY 4568 TATTATTTTGATTAC 5295 69 ARLP 4848 GCCAGACTTCCT5020 MVRG 4017 ATGGTAAGAGGT 5166 KR AAGAGA YYMDV 4686 TATTATATGGACGTC5296 70 ARAP 4849 GCCAGAGCCCCT 5021 TMVRG 4010 ACCATGGTGAGGGGT 5167 KVAAAGTG AEYFQH 4526 GCAGAGTACTTCCAGCAC 5297 71 ARTP 4850 GCCAGAACTCCT5022 RGV n/a AGAGGAGTT n/a LD TTGGAT DNWFDP 4594 GACAATTGGTTTGATCCC 529872 ARVP 4851 GCCAGAGTCCCT 5023 VRG n/a GTCAGAGGC n/a LE TTAGAG DWYFDL4530 GATTGGTACTTCGACTTG 5299 73 ARVGG 4852 GCCAGAGTTGGAGGA 5024 FG n/aTTCGGC n/a LG TTAGGT GNWFDP 4606 GGGAATTGGTTTGATCCT 5300 74 ARQ n/aGCAAGACAG n/a GE n/a GGCGAG n/a LH TTACAT NWYFDL 4532 AACTGGTATTTCGACTTA5301 75 ARH n/a GCCAGACAC n/a YG n/a TACGGC n/a LL TTATTG PNWFDP 4603CCCAATTGGTTTGATCCA 5302 76 ARDQ 4853 GCCAGGGACCAG 5025 VR n/a GTGCGT n/aLP TTACCA SNWFDP 4600 AGTAATTGGTTTGACCCC 5303 77 ARDH 4854 GCAAGAGACCAC5026 RG n/a AGAGGT n/a LR TTGAGG YNWFDP 4597 TATAATTGGTTTGATCCT 5304 78ARAR 4855 GCAAGGGCTAGA 5027 FRE n/a TTCAGGGAG n/a LS TTAAGC YWYFDL 4527TATTGGTATTTTGATTTG 5305 79 ARAS 4856 GCTAGGGCATCT 5028 RE n/a AGAGAG n/aLT TTGACA YYGMDV 4639 TACTATGGGATGGACGTG 5306 80 ARDQG 4857GCTAGGGATCAGGGA 5029 QG n/a CAAGGT n/a LV TTGGTA YYYMDV 4685TACTATTACATGGACGTT 5307 81 ARSR 4858 GCTAGATCAAGA 5030 LR n/a TTACGT n/aLW TTGTGG YYYGMDV 4638 TATTATTACGGTATGGACGTC 5308 82 ARDRS 4859GCCAGGGACAGGTCT 5031 YYDYVWGSYAYT 4070 TACTATGATTACGTCTGGGGGTCTTA 5168LY TTGTAC YYYYMDV 4684 TACTATTATTACATGGATGTC 5309 TGCTTACACT 83 ARSL4860 GCTAGATCTTTG 5032 YYDYVWGSYAY 4071 TACTACGACTATGTATGGGGCTCA 5169 MGATGGGC DYYYGMDV 4668 GACTATTATTACGGTATGGATGTT 5310 TATGCTTAC 84 ARLH4861 GCTAGGTTGCAC 5033 YYDYVWGSYA 4073 TACTACGATTACGTATGGGGAAGCTA 5170MT ATGACC GYYYGMDV 4677 GGCTACTATTATGGTATGGACGTC 5311 CGCT 85 ARDLT 4862GCCAGGGATTTGACT 5034 YDYVWGSYAY 4074 TATGATTATGTGTGGGGGTCATACGC 5171 PACCTGCT HYYYGMDV 4669 CATTACTACTATGGGATGGATGTA 5312 ATAC 86 ARK n/aGCCAGAAAG n/a DY n/a GATTAC n/a PD CCTGAT NYYYYMDV 4713AACTATTATTATTATATGGATGTC 5313 87 ARAE 4863 GCAAGAGCCGAG 5035 WG n/aTGGGGC n/a PE CCTGAG PYYYYMDV 4720 CCCTACTACTACTATATGGATGTG 5314 88ARDLS 4864 GCAAGGGATTTGTCT 5036 DYVWGSYAYT 4075GATTATGTGTGGGGGTCTTACGCCTA 5172 PF CCTTTC RYYYYMDV 4718AGGTATTACTACTACATGGACGTC 5315 CACC 89 ARGD 4865 GCTAGAGGGGAC 5037YDYVWGSYA 4077 TACGACTATGTGTGGGGTTCCTATGCT 5173 PG CCTGGT YYYYGMDV 4637TACTATTATTATGGGATGGATGTA 5316 90 ARRR 4866 GCTAGGAGGAGA 5038 YYDS 4183TACTACGATTCC 5174 PH CCACAT DYYYYGMDV 4655 GATTACTATTATTACGGAATGGAT 5317GTT 91 ARDK 4867 GCTAGAGATAAG 5039 YYYD 4182 TATTATTATGAC 5175 PL CCATTAGYYYYGMDV 4664 GGGTATTACTACTACGGCATGGAC 5318 GTA 92 ARVS 4868GCTAGAGTATCT 5040 DSSGY 4179 GACAGTTCCGGGTAC 5176 PP CCTCCA NYYYYGMDV4654 AATTACTATTACTATGGCATGGAT 5319 GTG 93 ARDRL 4869 GCCAGAGACAGGTTG5041 YDSSG 4178 TATGATAGCTCAGGT 5177 PQ CCTCAG PYYYYGMDV 4661CCATATTACTATTACGGCATGGAT 5320 GTC 94 ARGQ 4870 GCTAGGGGCCAG 5042 YYDSS4177 TACTATGACTCATCC 5178 PR CCAAGG SYYYYGMDV 4658AGCTACTACTACTACGGAATGGAC 5321 GTC 95 ARVR 4871 GCCAGGGTCAGA 5043 YYYDS4176 TATTATTACGATAGT 5179 PS CCTTCT YYYYYGMDV 4636TACTACTACTATTACGGTATGGAC 5322 GTA 96 ARAK 4872 GCTAGGGCTAAG 5044 GYY n/aGGATATTAC n/a PT CCTACA DYYYYYYMDV 4681 GATTATTATTACTACTACTACATG 5323GATGTA 97 ARGK 4873 GCCAGGGGTAAG 5045 DSSGYY 4174 GATTCTTCCGGGTACTAC5180 PV CCTGTT GYYYYYGMDV 4651 GGTTATTATTACTACTATGGGATG 5324 GATGTA 98ARDIS 4874 GCAAGGGATATTTCT 5046 YDSSGY 4173 TATGATTCCAGCGGATAC 5181 QGCAGGGC HYYYYYGMDV 4643 CACTACTATTATTATTACGGGAT 5325 GGATGTA 99 ARDFT4875 GCTAGGGATTTCACT 5047 YYDSSG 4172 TACTACGATAGCTCCGGT 5182 QL CAATTARYYYYYYMDV 4692 AGATACTACTACTATTATTACAT 5326 GGATGTA 100 ARQG 4876GCCAGGCAGGGA 5048 YYYDSS 4171 TATTATTACGACTCTTCC 5183 QP CAGCCAYYYYYYGMDV 4642 TATTACTACTATTACTATGGTAT 5327 GGACGTT 101 AK n/a GCCAAGn/a YDSSGYY 4169 TACGACTCTTCTGGTTATTAC 5184 QS CAGTCA 102 AKE n/aGCCAAGGAG n/a YYDSSGY 4168 TATTATGACAGCAGCGGGTAT 5185 QT CAGACT 103 AKDn/a GCCAAGGAC n/a YYYDSSG 4167 TACTACTACGATTCCAGCGGT 5186 RA AGGGCT 104AKG n/a GCCAAGGGA n/a YDSSGYYY 4166 TACGACAGTTCCGGATATTATTAC 5187 RDAGGGAC 105 AKEG 4877 GCCAAGGAAGGA 5049 SGY n/a AGCGGATAT n/a RE AGGGAG106 AKDG 4878 GCCAAGGACGGA 5050 YYDSSGYY 4165 TACTATGATAGTAGTGGGTACTAT5188 RF AGATTC 107 AKGG 4879 GCCAAGGGCGGA 5051 YYYDSSGY 4164TACTACTATGACAGCTCAGGGTAT 5189 RG AGGGGA 108 AKR n/a GCCAAGAGA n/aYYDSSGYYY 4163 TATTACGACAGCAGTGGCTACTACTAT 5190 RH AGGCAT 109 AKER 4880GCCAAGGAAAGA 5052 YYYDSSGYY 4162 TACTACTACGATAGCTCTGGATACTAT 5191 RLAGATTA 110 AKDR 4881 GCCAAGGACAGA 5053 YYYDSSGYYY 4161TATTATTACGATTCCAGTGGTTATTA 5192 RM AGGATG TTAT 111 AKGR 4882GCCAAGGGCAGA 5054 YDS n/a TACGACTCC n/a RP AGGCCA 112 AKS n/a GCCAAGTCTn/a YYD n/a TACTATGAC n/a RR CGTAGA 113 AKES 4883 GCCAAGGAATCT 5055 DSSG4185 GACTCATCCGGT 5193 RS AGAAGT 114 AKDS 4884 GCCAAGGATTCT 5056 GYYY4188 GGTTACTATTAC 5194 RV AGAGTG 115 AKGS 4885 GCCAAGGGATCT 5057 SGYY4187 AGCGGCTACTAT 5195 RY AGATAT 116 AKP n/a GCCAAGCCT n/a DS n/a GACTCTn/a SA TCAGCC 117 AKDP 4886 GCCAAGGATCCT 5058 RFLEW 4231 AGATTTTTGGAGTGG5196 SD TCAGAC 118 AKGP 4887 GCCAAGGGTCCT 5059 EWL n/a GAATGGCTT n/a SETCCGAG 119 AKL n/a GCCAAGTTG n/a RF n/a AGATTC n/a SF TCATTC 120 AKDL4888 GCCAAGGACTTG 5060 YYDFWSGYYT 4242 TACTATGATTTTTGGAGTGGATATTA 5197SG AGCGGA TACC 121 AKGL 4889 GCCAAGGGGTTG 5061 YDFWSG 4253TATGATTTTTGGTCTGGT 5198 SH AGTCAC 122 AKA n/a GCCAAGGCT n/a DFWSGY 4254GATTTTTGGAGCGGCTAT 5199 SI TCTATC 123 AKEA 4890 GCCAAGGAAGCT 5062 FWSGY4260 TTTTGGAGCGGGTAT 5200 SK AGTAAA 124 AKDA 4891 GCCAAGGACGCT 5063YYDFWSGYY 4243 TACTACGACTTCTGGAGCGGGTATTAC 5201 SL TCCTTG 125 AKGA 4892GCCAAGGGCGCT 5064 YYDFWSGY 4245 TACTACGATTTTTGGTCTGGATAT 5202 SP AGCCCA126 AKT n/a GCCAAGACT n/a YDFWSGYY 4246 TATGACTTTTGGAGTGGTTACTAC 5203 SQAGCCAA 127 AKET 4893 GCCAAGGAAACT 5065 YYDFWSG 4248TACTACGATTTCTGGTCAGGC 5204 SR TCAAGA 128 AKDT 4894 GCCAAGGATACT 5066YDFWSGY 4249 TATGACTTCTGGAGTGGTTAC 5205 SS TCCTCA 129 AKGT 4895GCCAAGGGAACT 5067 DFWSGYY 4250 GACTTCTGGTCAGGATACTAC 5206 ST AGTACA 130AKV n/a GCCAAGGTG n/a VLRYF 4307 GTGTTGAGGTACTTC 5207 SV TCAGTA 131 AKEV4896 GCCAAGGAAGTG 5068 LRYFD 4308 TTAAGATACTTTGAT 5208 SW TCATGG 132AKDV 4897 GCCAAGGACGTG 5069 RYFDW 4309 AGATACTTTGATTGG 5209 SY TCTTAC133 AKGV 4898 GCCAAGGGCGTG 5070 VLRY 4312 GTGTTGAGGTAT 5210 TA ACCGCC134 AKEGG 4899 GCCAAGGAGGGAGGA 5071 LRYF 4313 TTGAGATATTTC 5211 TGACTGGC 135 AKDGG 4900 GCCAAGGACGGTGGA 5072 RYFD 4314 AGATACTTTGAT 5212TP ACACCA 136 AKGGG 4901 GCCAAGGGAGGAGGA 5073 VLRYFDWL 4298GTCTTAAGGTACTTCGATTGGCTT 5213 TR ACAAGA 137 AKDGR 4902 GCCAAGGACGGTAGA5074 LRY n/a TTAAGATAC n/a TS ACATCT 138 AKGGS 4903 GCCAAGGGAGGTTCT 5075RYF n/a AGATACTTC n/a TT ACTACT 139 AKGGP 4904 GCCAAGGGAGGTCCT 5076 RYn/a AGATAT n/a TV ACAGTT 140 AKGGA 4905 GCCAAGGGAGGCGCT 5077 FD n/aTTCGAT n/a TW ACTTGG 141 AKDGT 4906 GCCAAGGATGGCACT 5078 DW n/a GATTGGn/a TY ACTTAT 142 AKGGT 4907 GCCAAGGGAGGCACT 5079 VLRYFDW 4300GTCTTAAGATACTTTGATTGG 5214 VA GTAGCC 143 AKEGV 4908 GCCAAGGAAGGAGTG 5080VLRYFD 4303 GTGTTGAGGTACTTTGAC 5215 VD GTCGAC 144 AKGGV 4909GCCAAGGGCGGTGTG 5081 LRYFDW 4304 TTAAGATACTTCGATTGG 5216 VG GTTGGA 145AKRG 4910 GCCAAGAGAGGA 5082 RYFDWL 4305 AGATATTTCGACTGGCTT 5217 VLGTCTTG 146 AKERG 4911 GCCAAGGAGAGAGGA 5083 YDILTGYY 4322TATGACATATTGACTGGCTACTAC 5218 VP GTTCCT 147 AKDRG 4912 GCCAAGGATAGGGGA5084 YDILTGY 4325 TATGATATATTAACTGGGTAC 5219 VR GTGAGA 148 AKGRG 4913GCCAAGGGTAGGGGA 5085 DILTGYY 4326 GATATCTTAACCGGGTATTAT 5220 VS GTTTCA149 AKSG 4914 GCCAAGTCTGGA 5086 DYG n/a GATTATGGT n/a VT GTTACC 150AKDSG 4915 GCCAAGGATAGTGGA 5087 GDY n/a GGGGACTAC n/a VV GTAGTA 151AKDPG 4916 GCCAAGGACCCCGGA 5088 YGD n/a TACGGTGAC n/a WG TGGGGT 152 AKLG4917 GCCAAGTTAGGA 5089 DYGD 4349 GATTACGGCGAT 5221 WS TGGTCA 153 AKDLG4918 GCCAAGGATCTTGGA 5090 YGDY 4350 TACGGCGATTAT 5222 YA TACGCT 154 AKAG4919 GCCAAGGCTGGA 5091 DYGDY 4348 GACTACGGAGATTAT 5223 YD TATGAC 155AKVG 4920 GCCAAGGTAGGA 5092 TTVTT 4351 ACCACAGTAACCACC 5224 YE TATGAG156 AKGVG 4921 GCCAAGGGTGTCGGA 5093 TTVT 4352 ACAACTGTGACT 5225 YSTATTCT 157 AKPR 4922 GCCAAGCCTAGA 5094 TVTT 4353 ACAGTAACTACT 5226 AAAGCTGCCGCT 158 AKGPR 4923 GCCAAGGGCCCCAGA 5095 RW n/a AGATGG n/a AGMGCAGGCATG 159 AKPS 4924 GCCAAGCCTTCT 5096 VTP n/a GTAACTCCT n/a DGGGATGGGGGT 160 AKDPS 4925 GCCAAGGATCCCTCT 5097 VD n/a GTTGAC n/a DGVGATGGTGTA 161 AKGPS 4926 GCCAAAGGGCCATCT 5098 GYSGYD 4386GGCTACTCAGGATACGAC 5227 FGG TTCGGGGGC 162 AKDPP 4927 GCCAAGGATCCACCT5099 YSGYD 4389 TATAGCGGATATGAC 5228 GAG GGAGCTGGA 163 AKGPP 4928GCCAAGGGCCCTCCT 5100 RD n/a AGAGAT n/a GGA GGAGGGGCC 164 AKPL 4929GCCAAGCCGTTG 5101 RDGY 4410 AGAGATGGTTAC 5229 GGG GGAGGCGGT 165 AKDPL4930 GCCAAGGACCCTTTG 5102 RDG n/a AGAGATGGT n/a GGL GGAGGCTTA 166 AKRP4931 GCCAAGAGGCCT 5103 DTAM 4420 GATACTGCTATG 5230 GGR GGTGGTAGA 167AKDRP 4932 GCCAAGGACCGTCCT 5104 YGY n/a TACGGCTAC n/a GGS GGCGGGAGC 168AKSP 4933 GCCAAGAGTCCT 5105 YSY n/a TATTCTTAC n/a GGV GGTGGGGTA 169 AKLP4934 GCCAAGCTACCT 5106 YSYG 4433 TATTCATATGGT 5231 GLG GGATTAGGC 170AKAP 4935 GCCAAGGCTCCT 5107 GYSYG 4430 GGATATAGTTATGGC 5232 GPGGGCCCCGGC 171 AKTP 4936 GCCAAGACGCCT 5108 SSS n/a AGTTCAAGC n/a GPPGGACCACCT 172 AKVP 4937 GCCAAGGTACCT 5109 YSSSWY 4437 TACAGTAGCTCTTGGTAC5233 GRG GGAAGGGGC 173 AKVGG 4938 GCCAAGGTGGGTGGA 5110 GYSSSWY 4435GGCTACAGTTCAAGCTGGTAT 5234 GSG GGATCTGGC 174 AKQ n/a GCAAAACAG n/a SSWn/a AGTTCCTGG n/a GTG GGAACTGGC 175 AKH n/a GCCAAACAC n/a SWY n/aAGCTGGTAC n/a GVG GGAGTAGGT 176 AKDQ 4939 GCCAAGGATCAG 5111 SSSW 4443AGTAGCTCTTGG 5235 LGG TTGGGAGGC 177 AKDH 4940 GCTAAAGACCAC 5112 YSSS4442 TACAGCAGCTCC 5236 LGH TTGGGCCAC 178 AKAR 4941 GCCAAGGCAAGA 5113YSSSW 4439 TACTCTTCCTCATGG 5237 PGG CCTGGCGGC 179 AKAS 4942 GCCAAGGCATCT5114 SW n/a AGCTGG n/a PKQ CCAAAGCAG 180 AKDQG 4943 GCAAAGGATCAGGGA 5115AG n/a GCTGGT n/a PKR CCTAAAAGG 181 AKSR 4944 GCCAAGAGTAGA 5116 QQLV4455 CAGCAATTGGTT 5238 PTQ CCAACTCAG 182 AKDRS 4945 GCAAAAGACAGGTCT 5117QQL n/a CAGCAACTT n/a RFE AGGTTTGAG 183 AKSL 4946 GCAAAGAGCTTG 5118 GWYn/a GGTTGGTAC n/a RGG AGGGGAGGT 184 AKLH 4947 GCTAAATTGCAC 5119 SSGWY4461 AGCTCTGGATGGTAC 5239 RGL AGGGGATTG 185 AKDLT 4948 GCCAAGGACTTGACT5120 YSSGW 4460 TATAGTAGCGGATGG 5240 RGS AGGGGTAGT 186 AKK n/a GCTAAAAAGn/a YSSGWY 4458 TATAGCAGCGGTTGGTAC 5241 RPL AGGCCATTA 187 AKAE 4949GCTAAAGCAGAG 5121 GYSSGWY 4456 GGTTATTCATCAGGTTGGTAT 5242 RPY AGGCCATAT188 AKDLS 4950 GCAAAAGACTTGTCT 5122 SGW n/a AGTGGTTGG n/a SAA TCTGCCGCC189 AKGD 4951 GCAAAAGGGGAC 5123 YSS n/a TACAGTTCC n/a SGE AGCGGGGAG 190AKRR 4952 GCAAAAAGGAGA 5124 SGWY 4464 AGTGGTTGGTAT 5243 SGG TCTGGCGGC191 AKDK 4953 GCAAAAGACAAG 5125 SSGW 4463 AGTTCCGGTTGG 5244 SGLTCAGGGTTA 192 AKVS 4954 GCCAAAGTATCT 5126 YSSG 4462 TACTCAAGTGGT 5245SGW AGCGGTTGG 193 AKDRL 4955 GCAAAAGACAGGTTG 5127 GW n/a GGTTGG n/a SGYAGCGGCTAC 194 AKGQ 4956 GCCAAAGGACAG 5128 QWLV 4475 CAGTGGTTAGTT 5246SRG TCAAGAGGT 195 AKVR 4957 GCAAAAGTCAGA 5129 EYSS 4479 GAGTACTCATCC5247 SSE TCATCAGAG 196 AKAK 4958 GCAAAGGCAAAG 5130 SIAARP 4481AGCATAGCAGCAAGGCCT 5248 SSW AGCTCATGG 197 AKGK 4959 GCAAAGGGCAAG 5131 RPn/a CGTCCT n/a TGG ACCGGTGGC 198 AKDIS 4960 GCAAAGGACATTTCT 5132 IAARP4483 ATAGCAGCAAGGCCT 5249 VGR GTTGGCAGA 199 AKDFT 4961 GCTAAAGATTTCACT5133 AARP 4486 GCCGCAAGACCT 5250 VQG GTGCAAGGA 200 AKQG 4962GCCAAGCAAGGA 5134 ARP n/a GCTAGACCT n/a VTA GTCACAGCT ¹“AR” and“AK” refer to the last two C-terminal amino acids of the heavy chainchassis used in the current example. They are not part of the TN1segment.

TABLE 26 Theoretical segment pool of 300 TN1 segments (plus AR/AK; whichis not part of TN1) used in the library of Example 12. Peptide SEQ SEQ(plus AR ID Nucleotide ID or AK)¹ NO (plus AR or AK)¹ NO In ELD-3? ARn/a GCCAGA n/a YES AK n/a GCCAAG n/a YES ARE n/a GCCAGAGAG n/a YES AKEn/a GCCAAGGAG n/a YES ARD n/a GCCAGAGAC n/a YES AKD n/a GCCAAGGAC n/aYES ARG n/a GCCAGAGGA n/a YES AKG n/a GCCAAGGGA n/a YES AREG 4791GCCAGAGAGGGA 4963 YES AKEG 4877 GCCAAGGAAGGA 5049 YES ARDG 4792GCCAGAGACGGA 4964 YES AKDG 4878 GCCAAGGACGGA 5050 YES ARGG 4793GCCAGAGGTGGA 4965 YES AKGG 4879 GCCAAGGGCGGA 5051 YES ARR n/a GCCAGGAGAn/a YES AKR n/a GCCAAGAGA n/a YES ARER 4794 GCCAGAGAGAGA 4966 YES AKER4880 GCCAAGGAAAGA 5052 YES ARDR 4795 GCCAGAGACAGA 4967 YES AKDR 4881GCCAAGGACAGA 5053 YES ARGR 4796 GCCAGAGGCAGA 4968 YES AKGR 4882GCCAAGGGCAGA 5054 YES ARS n/a GCCAGATCT n/a YES AKS n/a GCCAAGTCT n/aYES ARES 4797 GCCAGAGAATCT 4969 YES AKES 4883 GCCAAGGAATCT 5055 YES ARDS4798 GCCAGAGACTCT 4970 YES AKDS 4884 GCCAAGGATTCT 5056 YES ARGS 4799GCCAGAGGTTCT 4971 YES AKGS 4885 GCCAAGGGATCT 5057 YES ARP n/a GCCAGACCTn/a YES AKP n/a GCCAAGCCT n/a YES AREP 5328 GCCAGAGAGCCT 5428 NO AKEP5329 GCCAAGGAGCCT 5429 NO ARDP 4800 GCCAGAGACCCT 4972 YES AKDP 4886GCCAAGGATCCT 5058 YES ARGP 4801 GCCAGAGGGCCT 4973 YES AKGP 4887GCCAAGGGTCCT 5059 YES ARL n/a GCCAGATTG n/a YES AKL n/a GCCAAGTTG n/aYES AREL 5330 GCCAGAGAGTTG 5430 NO AKEL 5331 GCCAAGGAATTG 5431 NO ARDL4802 GCCAGAGACTTG 4974 YES AKDL 4888 GCCAAGGACTTG 5060 YES ARGL 4803GCCAGAGGGTTG 4975 YES AKGL 4889 GCCAAGGGGTTG 5061 YES ARA n/a GCCAGAGCTn/a YES AKA n/a GCCAAGGCT n/a YES AREA 4804 GCCAGAGAGGCT 4976 YES AKEA4890 GCCAAGGAAGCT 5062 YES ARDA 4805 GCCAGAGATGCT 4977 YES AKDA 4891GCCAAGGACGCT 5063 YES ARGA 4806 GCCAGAGGTGCT 4978 YES AKGA 4892GCCAAGGGCGCT 5064 YES ART n/a GCCAGAACT n/a YES AKT n/a GCCAAGACT n/aYES ARET 4807 GCCAGAGAGACT 4979 YES AKET 4893 GCCAAGGAAACT 5065 YES ARDT4808 GCCAGAGATACT 4980 YES AKDT 4894 GCCAAGGATACT 5066 YES ARGT 4809GCCAGAGGCACT 4981 YES AKGT 4895 GCCAAGGGAACT 5067 YES ARV n/a GCCAGAGTGn/a YES AKV n/a GCCAAGGTG n/a YES AREV 4810 GCCAGAGAGGTG 4982 YES AKEV4896 GCCAAGGAAGTG 5068 YES ARDV 4811 GCCAGAGATGTG 4983 YES AKDV 4897GCCAAGGACGTG 5069 YES ARGV 4812 GCCAGAGGGGTG 4984 YES AKGV 4898GCCAAGGGCGTG 5070 YES AREGG 4813 GCCAGAGAGGGAGGA 4985 YES AKEGG 4899GCCAAGGAGGGAGGA 5071 YES ARDGG 4814 GCCAGAGATGGTGGA 4986 YES AKDGG 4900GCCAAGGACGGTGGA 5072 YES ARGGG 4815 GCCAGAGGTGGAGGA 4987 YES AKGGG 4901GCCAAGGGAGGAGGA 5073 YES AREGR 5332 GCCAGAGAAGGGAGA 5432 NO AKEGR 5333GCCAAGGAAGGCAGA 5433 NO ARDGR 4816 GCCAGAGACGGCAGA 4988 YES AKDGR 4902GCCAAGGACGGTAGA 5074 YES ARGGR 5334 GCCAGAGGAGGTAGA 5434 NO AKGGR 5335GCCAAGGGAGGTAGA 5435 NO AREGS 5336 GCCAGAGAAGGATCT 5436 NO AKEGS 5337GCCAAGGAAGGATCT 5437 NO ARDGS 5338 GCCAGAGACGGATCT 5438 NO AKDGS 5339GCCAAGGATGGTTCT 5439 NO ARGGS 4817 GCCAGAGGCGGTTCT 4989 YES AKGGS 4903GCCAAGGGAGGTTCT 5075 YES AREGP 5340 GCCAGAGAAGGTCCT 5440 NO AKEGP 5341GCCAAGGAGGGGCCT 5441 NO ARDGP 5342 GCCAGAGACGGTCCT 5442 NO AKDGP 5343GCCAAGGACGGTCCT 5443 NO ARGGP 4818 GCCAGAGGTGGGCCT 4990 YES AKGGP 4904GCCAAGGGAGGTCCT 5076 YES AREGL 5344 GCCAGAGAGGGCTTG 5444 NO AKEGL 5345GCCAAGGAAGGGTTG 5445 NO ARDGL 5346 GCCAGAGATGGGTTG 5446 NO AKDGL 5347GCCAAGGACGGTTTG 5447 NO ARGGL 5348 GCCAGAGGTGGATTG 5448 NO AKGGL 5349GCCAAGGGAGGGTTG 5449 NO AREGA 5350 GCCAGAGAAGGAGCT 5450 NO AKEGA 5351GCCAAGGAGGGAGCT 5451 NO ARDGA 5352 GCCAGAGATGGCGCT 5452 NO AKDGA 5353GCCAAGGATGGAGCT 5453 NO ARGGA 4819 GCCAGAGGAGGTGCT 4991 YES AKGGA 4905GCCAAGGGAGGCGCT 5077 YES ARDGT 4820 GCCAGAGACGGTACT 4992 YES AKDGT 4906GCCAAGGATGGCACT 5078 YES ARGGT 4821 GCCAGAGGTGGAACT 4993 YES AKGGT 4907GCCAAGGGAGGCACT 5079 YES AREGV 4822 GCCAGAGAGGGAGTG 4994 YES AKEGV 4908GCCAAGGAAGGAGTG 5080 YES ARDGV 5354 GCCAGAGATGGTGTG 5454 NO AKDGV 5355GCCAAGGATGGTGTG 5455 NO ARGGV 4823 GCCAGAGGTGGCGTG 4995 YES AKGGV 4909GCCAAGGGCGGTGTG 5081 YES ARRG 4824 GCCAGAAGAGGA 4996 YES AKRG 4910GCCAAGAGAGGA 5082 YES ARERG 4825 GCCAGAGAGCGTGGA 4997 YES AKERG 4911GCCAAGGAGAGAGGA 5083 YES ARDRG 4826 GCCAGAGATCGTGGA 4998 YES AKDRG 4912GCCAAGGATAGGGGA 5084 YES ARGRG 4827 GCCAGAGGCAGGGGA 4999 YES AKGRG 4913GCCAAGGGTAGGGGA 5085 YES ARSG 4828 GCCAGATCAGGA 5000 YES AKSG 4914GCCAAGTCTGGA 5086 YES ARESG 5356 GCCAGAGAGTCTGGA 5456 NO AKESG 5357GCCAAGGAAAGTGGA 5457 NO ARDSG 4829 GCCAGAGACTCAGGA 5001 YES AKDSG 4915GCCAAGGATAGTGGA 5087 YES ARGSG 5358 GCCAGAGGCTCTGGA 5458 NO AKGSG 5359GCCAAGGGGTCTGGA 5459 NO ARPG 5360 GCCAGACCAGGA 5460 NO AKPG 5361GCCAAGCCCGGA 5461 NO ARDPG 4830 GCCAGAGATCCAGGA 5002 YES AKDPG 4916GCCAAGGACCCCGGA 5088 YES ARGPG 5362 GCCAGAGGACCTGGA 5462 NO AKGPG 5363GCCAAGGGGCCTGGA 5463 NO ARLG 4831 GCCAGATTGGGA 5003 YES AKLG 4917GCCAAGTTAGGA 5089 YES ARDLG 4832 GCCAGAGACTTGGGA 5004 YES AKDLG 4918GCCAAGGATCTTGGA 5090 YES ARGLG 5364 GCCAGAGGACTAGGA 5464 NO AKGLG 5365GCCAAGGGTTTGGGA 5465 NO ARAG 4833 GCCAGAGCTGGA 5005 YES AKAG 4919GCCAAGGCTGGA 5091 YES AREAG 5366 GCCAGAGAAGCCGGA 5466 NO AKEAG 5367GCCAAGGAGGCTGGA 5467 NO ARDAG 5368 GCCAGAGACGCAGGA 5468 NO AKDAG 5369GCCAAGGATGCCGGA 5469 NO ARGAG 5370 GCCAGAGGTGCCGGA 5470 NO AKGAG 5371GCCAAGGGAGCAGGA 5471 NO ARTG 5372 GCCAGAACTGGA 5472 NO AKTG 5373GCCAAGACCGGA 5473 NO ARDTG 5374 GCCAGAGACACGGGA 5474 NO AKDTG 5375GCCAAGGATACGGGA 5475 NO ARVG 4834 GCCAGAGTGGGA 5006 YES AKVG 4920GCCAAGGTAGGA 5092 YES AREVG 5376 GCCAGAGAAGTCGGA 5476 NO AKEVG 5377GCCAAGGAGGTAGGA 5477 NO ARDVG 5378 GCCAGAGATGTAGGA 5478 NO AKDVG 5379GCCAAGGACGTAGGA 5479 NO ARGVG 4835 GCCAGAGGCGTAGGA 5007 YES AKGVG 4921GCCAAGGGTGTCGGA 5093 YES ARPR 4836 GCCAGACCCAGA 5008 YES AKPR 4922GCCAAGCCTAGA 5094 YES ARDPR 5380 GCCAGAGATCCAAGA 5480 NO AKDPR 5381GCCAAGGATCCTAGA 5481 NO ARGPR 4837 GCCAGAGGACCAAGA 5009 YES AKGPR 4923GCCAAGGGCCCCAGA 5095 YES ARPS 4838 GCCAGACCATCT 5010 YES AKPS 4924GCCAAGCCTTCT 5096 YES ARDPS 4839 GCCAGAGATCCCTCT 5011 YES AKDPS 4925GCCAAGGATCCCTCT 5097 YES ARGPS 4840 GCAAGAGGACCTTCT 5012 YES AKGPS 4926GCCAAAGGGCCATCT 5098 YES ARPP 5382 GCCAGACCACCT 5482 NO AKPP 5383GCCAAGCCACCT 5483 NO ARDPP 4841 GCCAGAGACCCACCT 5013 YES AKDPP 4927GCCAAGGATCCACCT 5099 YES ARGPP 4842 GCCAGAGGACCGCCT 5014 YES AKGPP 4928GCCAAGGGCCCTCCT 5100 YES ARPL 4843 GCCAGACCGTTG 5015 YES AKPL 4929GCCAAGCCGTTG 5101 YES ARDPL 4844 GCCAGAGATCCTTTG 5016 YES AKDPL 4930GCCAAGGACCCTTTG 5102 YES ARGPL 5384 GCCAGAGGTCCCTTG 5484 NO AKGPL 5385GCCAAGGGGCCGTTG 5485 NO ARPA 5386 GCCAGACCAGCT 5486 NO AKPA 5387GCCAAGCCGGCT 5487 NO ARDPA 5388 GCCAGAGATCCCGCT 5488 NO AKDPA 5389GCCAAGGACCCCGCT 5489 NO ARPT 5390 GCCAGACCTACT 5490 NO AKPT 5391GCCAAGCCTACT 5491 NO ARDPT 5392 GCCAGAGATCCGACT 5492 NO AKDPT 5393GCCAAGGACCCTACT 5493 NO ARGPT 5394 GCCAGAGGACCCACT 5494 NO AKGPT 5395GCCAAGGGGCCCACT 5495 NO ARPV 5396 GCCAGACCGGTG 5496 NO AKPV 5397GCCAAGCCAGTG 5497 NO ARDPV 5398 GCCAGAGATCCGGTG 5498 NO AKDPV 5399GCCAAGGACCCTGTG 5499 NO ARRP 4845 GCCAGAAGGCCT 5017 YES AKRP 4931GCCAAGAGGCCT 5103 YES ARDRP 4846 GCCAGAGACCGTCCT 5018 YES AKDRP 4932GCCAAGGACCGTCCT 5104 YES ARGRP 5400 GCCAGAGGAAGGCCT 5500 NO AKGRP 5401GCCAAGGGCCGTCCT 5501 NO ARSP 4847 GCCAGATCACCT 5019 YES AKSP 4933GCCAAGAGTCCT 5105 YES ARDSP 5402 GCCAGAGACTCTCCT 5502 NO AKDSP 5403GCCAAGGACTCCCCT 5503 NO ARGSP 5404 GCCAGAGGTTCCCCT 5504 NO AKGSP 5405GCCAAGGGTTCACCT 5505 NO ARLP 4848 GCCAGACTTCCT 5020 YES AKLP 4934GCCAAGCTACCT 5106 YES ARDLP 5406 GCCAGAGATCTTCCT 5506 NO AKDLP 5407GCCAAGGATCTACCT 5507 NO ARAP 4849 GCCAGAGCCCCT 5021 YES AKAP 4935GCCAAGGCTCCT 5107 YES ARDAP 5408 GCCAGAGATGCTCCT 5508 NO AKDAP 5409GCCAAGGATGCTCCT 5509 NO ARGAP 5410 GCCAGAGGGGCCCCT 5510 NO AKGAP 5411GCCAAGGGTGCCCCT 5511 NO ARTP 4850 GCCAGAACTCCT 5022 YES AKTP 4936GCCAAGACGCCT 5108 YES ARDTP 5412 GCCAGAGATACCCCT 5512 NO AKDTP 5413GCCAAGGACACGCCT 5513 NO ARVP 4851 GCCAGAGTCCCT 5023 YES AKVP 4937GCCAAGGTACCT 5109 YES ARAGG 5414 GCCAGAGCTGGCGGA 5514 NO AKAGG 5415GCCAAGGCCGGTGGA 5515 NO ARDGGG 5416 GCCAGAGATGGTGGCGGA 5516 NO AKDGGG5417 GCCAAGGACGGCGGTGGA 5517 NO ARLGG 5418 GCCAGATTGGGCGGA 5518 NO AKLGG5419 GCCAAGCTAGGCGGA 5519 NO ARDLGG 5420 GCCAGAGATTTGGGTGGA 5520 NOAKDLGG 5421 GCCAAGGATTTGGGTGGA 5521 NO ARRGG 5422 GCCAGAAGAGGTGGA 5522NO AKRGG 5423 GCCAAGAGAGGAGGA 5523 NO ARDRGG 5424 GCCAGAGACCGTGGCGGA5524 NO AKDRGG 5425 GCCAAGGACAGAGGTGGA 5525 NO ARSGG 5426GCCAGATCAGGCGGA 5526 NO AKSGG 5427 GCCAAGTCCGGTGGA 5527 NO ARVGG 4852GCCAGAGTTGGAGGA 5024 YES AKVGG 4938 GCCAAGGTGGGTGGA 5110 YES ARQ n/aGCAAGACAG n/a YES AKQ n/a GCAAAACAG n/a YES ARH n/a GCCAGACAC n/a YESAKH n/a GCCAAACAC n/a YES ARDQ 4853 GCCAGGGACCAG 5025 YES AKDQ 4939GCCAAGGATCAG 5111 YES ARDH 4854 GCAAGAGACCAC 5026 YES AKDH 4940GCTAAAGACCAC 5112 YES ARAR 4855 GCAAGGGCTAGA 5027 YES AKAR 4941GCCAAGGCAAGA 5113 YES ARAS 4856 GCTAGGGCATCT 5028 YES AKAS 4942GCCAAGGCATCT 5114 YES ARDQG 4857 GCTAGGGATCAGGGA 5029 YES AKDQG 4943GCAAAGGATCAGGGA 5115 YES ARSR 4858 GCTAGATCAAGA 5030 YES AKSR 4944GCCAAGAGTAGA 5116 YES ARDRS 4859 GCCAGGGACAGGTCT 5031 YES AKDRS 4945GCAAAAGACAGGTCT 5117 YES ARSL 4860 GCTAGATCTTTG 5032 YES AKSL 4946GCAAAGAGCTTG 5118 YES ARLH 4861 GCTAGGTTGCAC 5033 YES AKLH 4947GCTAAATTGCAC 5119 YES ARDLT 4862 GCCAGGGATTTGACT 5034 YES AKDLT 4948GCCAAGGACTTGACT 5120 YES ARK n/a GCCAGAAAG n/a YES AKK n/a GCTAAAAAG n/aYES ARAE 4863 GCAAGAGCCGAG 5035 YES AKAE 4949 GCTAAAGCAGAG 5121 YESARDLS 4864 GCAAGGGATTTGTCT 5036 YES AKDLS 4950 GCAAAAGACTTGTCT 5122 YESARGD 4865 GCTAGAGGGGAC 5037 YES AKGD 4951 GCAAAAGGGGAC 5123 YES ARRR4866 GCTAGGAGGAGA 5038 YES AKRR 4952 GCAAAAAGGAGA 5124 YES ARDK 4867GCTAGAGATAAG 5039 YES AKDK 4953 GCAAAAGACAAG 5125 YES ARVS 4868GCTAGAGTATCT 5040 YES AKVS 4954 GCCAAAGTATCT 5126 YES ARDRL 4869GCCAGAGACAGGTTG 5041 YES AKDRL 4955 GCAAAAGACAGGTTG 5127 YES ARGQ 4870GCTAGGGGCCAG 5042 YES AKGQ 4956 GCCAAAGGACAG 5128 YES ARVR 4871GCCAGGGTCAGA 5043 YES AKVR 4957 GCAAAAGTCAGA 5129 YES ARAK 4872GCTAGGGCTAAG 5044 YES AKAK 4958 GCAAAGGCAAAG 5130 YES ARGK 4873GCCAGGGGTAAG 5045 YES AKGK 4959 GCAAAGGGCAAG 5131 YES ARDIS 4874GCAAGGGATATTTCT 5046 YES AKDIS 4960 GCAAAGGACATTTCT 5132 YES ARDFT 4875GCTAGGGATTTCACT 5047 YES AKDFT 4961 GCTAAAGATTTCACT 5133 YES ARQG 4876GCCAGGCAGGGA 5048 YES AKQG 4962 GCCAAGCAAGGA 5134 YES ¹“AR” and“AK” refer to the last two C-terminal amino acids of the heavy chainchassis used in the current example. They are not part of the TN1segment.

TABLE 27 Theoretical segment pool of degenerate oligonucleotidesequences encoding DH segments of Example 13. Degenerate Peptide SEQ IDName Oligo Length NO DH_001 KHTGAK 2 n/a DH_002 KHTKGG 2 n/a DH_003KHTCMT 2 n/a DH_004 KHTMCT 2 n/a DH_005 GVCWSG 2 n/a DH_006 SVCYAT 2 n/aDH_007 BYCSAG 2 n/a DH_008 SBAMAG 2 n/a DH_009 VSCMAA 2 n/a DH_010GRABYT 2 n/a DH_011 GRAKBG 2 n/a DH_012 RDAGAK 2 n/a DH_013 RDAGRT 2 n/aDH_014 YHTSAC 2 n/a DH_015 YHTKAC 2 n/a DH_016 YHTMCG 2 n/a DH_017MHAGAW 2 n/a DH_018 MHAGRT 2 n/a DH_019 MHAMCT 2 n/a DH_020 MBCYAT 2 n/aDH_021 CVACNG 2 n/a DH_022 MSCAHG 2 n/a DH_023 CRGKBG 2 n/a DH_024WSGHCT 2 n/a DH_025 WGGKHT 2 n/a DH_026 BGGSAK 2 n/a DH_027 BWCAMA 2 n/aDH_028 BHCTGG 2 n/a DH_029 TGGVBT 2 n/a DH_030 BHCAGT 2 n/a DH_031SRTATT 2 n/a DH_032 ACABHT 2 n/a DH_033 SVCGCT 2 n/a DH_034 ATGSVT 2 n/aDH_035 SWGAGG 2 n/a DH_036 GTAGCAVBT 3 n/a DH_037 DBGSWACTT 3 n/a DH_038VNCBCAGGT 3 n/a DH_039 VNCDCATAT 3 n/a DH_040 VHAKKGTTG 3 n/a DH_041CCAGCABHT 3 n/a DH_042 VHASRACTT 3 n/a DH_043 BHCAGCRST 3 n/a DH_044BHCGGAKMT 3 n/a DH_045 BHCGGAGDT 3 n/a DH_046 BHCAGCKMT 3 n/a DH_047NHCCRACTT 3 n/a DH_048 NHCAGCKGG 3 n/a DH_049 BHCGGAKSG 3 n/a DH_050VBCGGAGNT 3 n/a DH_051 NHCAGCGVT 3 n/a DH_052 NHCTACGVT 3 n/a DH_053NHCAGCGVG 3 n/a DH_054 VHATGGSYG 3 n/a DH_055 VNCGHCTAT 3 n/a DH_056GGTRNACTT 3 n/a DH_057 NHCABAGGT 3 n/a DH_058 VHAGCAGNT 3 n/a DH_059DBGKYCGGT 3 n/a DH_060 BHCGGARKT 3 n/a DH_061 NHCGTAGVT 3 n/a DH_062VNTTHCTAT 3 n/a DH_063 GTAGTABHT 3 n/a DH_064 VBCGNCCTT 3 n/a DH_065BHCGGAGNG 3 n/a DH_066 VNCGHCGGT 3 n/a DH_067 AGGBHCGGT 3 n/a DH_068VNCTBGTAT 3 n/a DH_069 VNCTBGCTT 3 n/a DH_070 NHCKACTAT 3 n/a DH_071CTARNACTT 3 n/a DH_072 NHCBCAGGT 3 n/a DH_073 NHCTACBAT 3 n/a DH_074BHCACAGCCAKS 4 5528 DH_075 VHGGBAGCAACT 4 5529 DH_076 DBGTTCGGAGNG 45530 DH_077 BHCGGAKMCTAT 4 5531 DH_078 TACAGCAGCVBT 4 5532 DH_079VHGGTARSAGGT 4 5533 DH_080 VBCGACGGATHT 4 5534 DH_081 NHCTACGGAGVT 45535 DH_082 VHGRYGGCAACT 4 5536 DH_083 VHATACAGCRST 4 5537 DH_084CAGTGGCTABHT 4 5538 DH_085 VHAGTAGCAGNT 4 5539 DH_086 VBCACAGTARMG 45540 DH_087 AGCAGCAGCDBG 4 5541 DH_088 NHCTMCTACGGT 4 5542 DH_089BHCAGCTGGTHT 4 5543 DH_090 VHACAACTAGNT 4 5544 DH_091 BHCGGAAGCKMT 45545 DH_092 BHCGGATSGTAT 4 5546 DH_093 NHCAGCGGABGG 4 5547 DH_094BHCGGATACKMT 4 5548 DH_095 VHAGTAACARMG 4 5549 DH_096 GCAGCAGCAVBT 45550 DH_097 VBCAYATTCGGT 4 5551 DH_098 GTAGCAGCAVHA 4 5552 DH_099NHCTACTACGVT 4 5553 DH_100 VBCKMCGGATAT 4 5554 DH_101 VHACAACTAKKG 45555 DH_102 VHGGGARKCGCT 4 5556 DH_103 VBTBTCGGAGAG 4 5557 DH_104BHCTACAGCKMT 4 5558 DH_105 VHAGTASSAGCT 4 5559 DH_106 VBTCDAGGAGTT 45560 DH_107 GACAGCAGCDBG 4 5561 DH_108 VBCGVCTACAGT 4 5562 DH_109NHCTACGGAKCT 4 5563 DH_110 NHCTACTACTHT 4 5564 DH_111 BHCVGCTACAGT 45565 DH_112 VBCTGGTTCGGT 4 5566 DH_113 VNCTACTACTHT 4 5567 DH_114VHABTCGGAGGT 4 5568 DH_115 NHCATGGTAAGAGVT 5 5569 DH_116 NHCTACGGAGACTHT5 5570 DH_117 VBCTACAGCTACGNT 5 5571 DH_118 VNCAGCGGAAGCTHT 5 5572DH_119 VBCTTTCTAGAATBG 5 5573 DH_120 BHCGGAAGCTACKMT 5 5574 DH_121NHCAGCGGAAGCTHT 5 5575 DH_122 BHCAGCAGCAGCTBG 5 5576 DH_123BHCAGCAGCGGATBG 5 5577 DH_124 VBCACAGTAACAANA 5 5578 DH_125BHCAGCAGCAGCTHT 5 5579 DH_126 NHCAGCGGATGGTHT 5 5580 DH_127NHCAGCGGATACGVT 5 5581 DH_128 NHCTACTACGACABT 5 5582 DH_129BHCAGCAGCGGATHT 5 5583 DH_130 VHAGCAGCAAGACNT 5 5584 DH_131VHGTACTACTACGVT 5 5585 DH_132 VBCACAATGGTACRG 5 5586 DH_133NHCATGGTACRAGGT 5 5587 DH_134 NHCTSGGGAAGCTAT 5 5588 DH_135BHCAGCAGCAGCTGGTHT 6 5589 DH_136 NHCTACTACGACAGCABT 6 5590 DH_137VBCTACAGCGGATACGNT 6 5591 DH_138 BHCAGCAGCGGATGGTHT 6 5592 DH_139NHCTACTACGGAAGCGVT 6 5593 DH_140 BHCAGCAGCGGATACTHT 6 5594 DH_141NHCTACGACAGCAGCGVT 6 5595 DH_142 NHCGACTTCTGGAGCGVT 6 5596 DH_143BHCGACAGCAGCGGATHT 6 5597 DH_144 SNATACTTCGACTGGYYT 6 5598 DH_145TGTRGCRGCACAAGCTGT 6 5599 DH_146 NHCTACTACGGAAGCGVG 6 5600 DH_147TGTRGCRGCGGAAGCTGT 6 5601 DH_148 NHCTTTTGGAGCGGATHT 6 5602 DH_149NHCTACTACGACAGCAGCGVT 7 5603 DH_150 NHCGACATACTAACAGGATHT 7 5604 DH_151NHCTACGACTTCTGGAGCGVT 7 5605 DH_152 BHCTGTAGCAGCACAAGCTGT 7 5606 DH_153DBGTACAGCAGCAGCTGGTHT 7 5607 DH_154 NHCTACGACAGCAGCGGATHT 7 5608 DH_155NHCTACTACGGAAGCGGAABT 7 5609 DH_156 DBGTACAGCAGCGGATGGTHT 7 5610 DH_157BHCTGTAGCGGAGGAAGCTGT 7 5611 DH_158 TGTAGCGGAGGAAGCTGTYHT 7 5612 DH_159TGTAGCAGCACAAGCTGTYHT 7 5613 DH_160 NHCTGTGGAGGAGACTGTTHT 7 5614 DH_161NHCGACTTCTGGAGCGGATHT 7 5615 DH_162 BHCGACAGCAGCGGATACTHT 7 5616 DH_163VHATACTGTGGAGGAGACTGT 7 5617 DH_164 NHCTACTACGACAGCAGCGGATHT 8 5618DH_165 NHCTACTACGGAAGCGGAAGCTHT 8 5619 DH_166 VBCTACTGTAGCAGCACAAGCTGT 85620 DH_167 VBCTACTGTAGCGGAGGAAGCTGT 8 5621 DH_168NHCTACGACTTCTGGAGCGGATHT 8 5622 DH_169 NHCGACATACTAACAGGATACTHT 8 5623DH_170 BHCTGTAGCGGAGGAAGCTGTTHT 8 5624 DH_171 BHCTGTAGCAGCACAAGCTGTTHT 85625 DH_172 VHATACTGTGGAGGAGACTGTTHT 8 5626 DH_173NHCTACGACAGCAGCGGATACTHT 8 5627 DH_174 VNCTACTACGGAAGCGGAAGCTMT 8 5628DH_175 NHCGACTTCTGGAGCGGATACTHT 8 5629 DH_176 VHACTAAGATACTTCGACTGGYWT 85630 DH_177 NHCTACTACGACAGCAGCGGATACTHT 9 5631 DH_178VBCTACTGTAGCGGAGGAAGCTGTTHT 9 5632 DH_179 VBCTACTGTAGCAGCACAAGCTGTTHT 95633 DH_180 NHCTACGACTTCTGGAGCGGATACTHT 9 5634 DH_181BHCTGTAGCGGAGGAAGCTGTTACTHT 9 5635 DH_182 NHCTACTACGACAGCAGCGGATACT 105636 ACTHT DH_183 NHCTACGACTACGTATGGGGAAGCTAC 11 5637 GCATHT DH_184NHCTACGACTACGTATGGGGAAGCTACGC 12 5638 ATACAHA

TABLE 28 Theoretical segment pool of unique DH polypeptide segmentsencoded by the degenerate oligonucleotides of Table 27. Name SequenceLength SEQ ID NO PDH_0001 YE 2 n/a PDH_0002 DD 2 n/a PDH_0003 VD 2 n/aPDH_0004 FD 2 n/a PDH_0005 AE 2 n/a PDH_0006 SD 2 n/a PDH_0007 YD 2 n/aPDH_0008 VE 2 n/a PDH_0009 DE 2 n/a PDH_0010 AD 2 n/a PDH_0011 FE 2 n/aPDH_0012 SE 2 n/a PDH_0013 VG 2 n/a PDH_0014 FW 2 n/a PDH_0015 YG 2 n/aPDH_0016 DW 2 n/a PDH_0017 FG 2 n/a PDH_0018 AW 2 n/a PDH_0019 DG 2 n/aPDH_0020 YW 2 n/a PDH_0021 SG 2 n/a PDH_0022 AG 2 n/a PDH_0023 VW 2 n/aPDH_0024 SW 2 n/a PDH_0025 VP 2 n/a PDH_0026 DH 2 n/a PDH_0027 DP 2 n/aPDH_0028 YP 2 n/a PDH_0029 SH 2 n/a PDH_0030 VH 2 n/a PDH_0031 FH 2 n/aPDH_0032 YH 2 n/a PDH_0033 FP 2 n/a PDH_0034 AP 2 n/a PDH_0035 SP 2 n/aPDH_0036 AH 2 n/a PDH_0037 YT 2 n/a PDH_0038 DT 2 n/a PDH_0039 AT 2 n/aPDH_0040 ST 2 n/a PDH_0041 FT 2 n/a PDH_0042 VT 2 n/a PDH_0043 AS 2 n/aPDH_0044 AR 2 n/a PDH_0045 DS 2 n/a PDH_0046 GT 2 n/a PDH_0047 GS 2 n/aPDH_0048 GW 2 n/a PDH_0049 GR 2 n/a PDH_0050 DR 2 n/a PDH_0051 PH 2 n/aPDH_0052 RH 2 n/a PDH_0053 PY 2 n/a PDH_0054 GH 2 n/a PDH_0055 GY 2 n/aPDH_0056 RY 2 n/a PDH_0057 HH 2 n/a PDH_0058 HY 2 n/a PDH_0059 DY 2 n/aPDH_0060 AY 2 n/a PDH_0061 AQ 2 n/a PDH_0062 FQ 2 n/a PDH_0063 LE 2 n/aPDH_0064 PE 2 n/a PDH_0065 LQ 2 n/a PDH_0066 PQ 2 n/a PDH_0067 VQ 2 n/aPDH_0068 SQ 2 n/a PDH_0069 RK 2 n/a PDH_0070 GK 2 n/a PDH_0071 AK 2 n/aPDH_0072 RQ 2 n/a PDH_0073 GQ 2 n/a PDH_0074 LK 2 n/a PDH_0075 VK 2 n/aPDH_0076 PK 2 n/a PDH_0077 SK 2 n/a PDH_0078 TK 2 n/a PDH_0079 TQ 2 n/aPDH_0080 GL 2 n/a PDH_0081 GP 2 n/a PDH_0082 GV 2 n/a PDH_0083 EF 2 n/aPDH_0084 GF 2 n/a PDH_0085 EL 2 n/a PDH_0086 EA 2 n/a PDH_0087 ES 2 n/aPDH_0088 EP 2 n/a PDH_0089 GA 2 n/a PDH_0090 EV 2 n/a PDH_0091 GG 2 n/aPDH_0092 EG 2 n/a PDH_0093 EW 2 n/a PDH_0094 IE 2 n/a PDH_0095 RE 2 n/aPDH_0096 KE 2 n/a PDH_0097 GD 2 n/a PDH_0098 ID 2 n/a PDH_0099 RD 2 n/aPDH_0100 EE 2 n/a PDH_0101 GE 2 n/a PDH_0102 KD 2 n/a PDH_0103 ED 2 n/aPDH_0104 IG 2 n/a PDH_0105 RG 2 n/a PDH_0106 KG 2 n/a PDH_0107 LD 2 n/aPDH_0108 LH 2 n/a PDH_0109 PD 2 n/a PDH_0110 HD 2 n/a PDH_0111 SY 2 n/aPDH_0112 FY 2 n/a PDH_0113 YY 2 n/a PDH_0114 LY 2 n/a PDH_0115 LT 2 n/aPDH_0116 HP 2 n/a PDH_0117 HT 2 n/a PDH_0118 LP 2 n/a PDH_0119 PT 2 n/aPDH_0120 PP 2 n/a PDH_0121 TE 2 n/a PDH_0122 QE 2 n/a PDH_0123 TD 2 n/aPDH_0124 QD 2 n/a PDH_0125 PG 2 n/a PDH_0126 LG 2 n/a PDH_0127 TG 2 n/aPDH_0128 QG 2 n/a PDH_0129 QP 2 n/a PDH_0130 QT 2 n/a PDH_0131 KT 2 n/aPDH_0132 KP 2 n/a PDH_0133 IP 2 n/a PDH_0134 TP 2 n/a PDH_0135 TT 2 n/aPDH_0136 IT 2 n/a PDH_0137 IH 2 n/a PDH_0138 IY 2 n/a PDH_0139 TH 2 n/aPDH_0140 TY 2 n/a PDH_0141 RR 2 n/a PDH_0142 QL 2 n/a PDH_0143 QQ 2 n/aPDH_0144 PL 2 n/a PDH_0145 RP 2 n/a PDH_0146 PR 2 n/a PDH_0147 RL 2 n/aPDH_0148 QR 2 n/a PDH_0149 PM 2 n/a PDH_0150 TM 2 n/a PDH_0151 RT 2 n/aPDH_0152 RM 2 n/a PDH_0153 SM 2 n/a PDH_0154 QA 2 n/a PDH_0155 RA 2 n/aPDH_0156 QS 2 n/a PDH_0157 QV 2 n/a PDH_0158 RS 2 n/a PDH_0159 QW 2 n/aPDH_0160 RW 2 n/a PDH_0161 RV 2 n/a PDH_0162 WS 2 n/a PDH_0163 WT 2 n/aPDH_0164 TS 2 n/a PDH_0165 WP 2 n/a PDH_0166 SS 2 n/a PDH_0167 WV 2 n/aPDH_0168 WF 2 n/a PDH_0169 RF 2 n/a PDH_0170 WA 2 n/a PDH_0171 WD 2 n/aPDH_0172 WY 2 n/a PDH_0173 WQ 2 n/a PDH_0174 WE 2 n/a PDH_0175 WH 2 n/aPDH_0176 YK 2 n/a PDH_0177 FK 2 n/a PDH_0178 DK 2 n/a PDH_0179 HK 2 n/aPDH_0180 LW 2 n/a PDH_0181 PW 2 n/a PDH_0182 HW 2 n/a PDH_0183 WI 2 n/aPDH_0184 WG 2 n/a PDH_0185 WL 2 n/a PDH_0186 WR 2 n/a PDH_0187 YS 2 n/aPDH_0188 LS 2 n/a PDH_0189 HS 2 n/a PDH_0190 FS 2 n/a PDH_0191 PS 2 n/aPDH_0192 VS 2 n/a PDH_0193 GI 2 n/a PDH_0194 HI 2 n/a PDH_0195 RI 2 n/aPDH_0196 DI 2 n/a PDH_0197 TF 2 n/a PDH_0198 TL 2 n/a PDH_0199 TV 2 n/aPDH_0200 TA 2 n/a PDH_0201 PA 2 n/a PDH_0202 HA 2 n/a PDH_0203 DA 2 n/aPDH_0204 AA 2 n/a PDH_0205 MR 2 n/a PDH_0206 MA 2 n/a PDH_0207 MD 2 n/aPDH_0208 MP 2 n/a PDH_0209 MH 2 n/a PDH_0210 MG 2 n/a PDH_0211 VR 2 n/aPDH_0212 ER 2 n/a PDH_0213 LR 2 n/a PDH_0214 VAL 3 n/a PDH_0215 VAR 3n/a PDH_0216 VAI 3 n/a PDH_0217 VAA 3 n/a PDH_0218 VAT 3 n/a PDH_0219VAP 3 n/a PDH_0220 VAV 3 n/a PDH_0221 VAG 3 n/a PDH_0222 VAS 3 n/aPDH_0223 VVL 3 n/a PDH_0224 VEL 3 n/a PDH_0225 REL 3 n/a PDH_0226 TLL 3n/a PDH_0227 WEL 3 n/a PDH_0228 RLL 3 n/a PDH_0229 TQL 3 n/a PDH_0230RVL 3 n/a PDH_0231 GLL 3 n/a PDH_0232 TEL 3 n/a PDH_0233 GVL 3 n/aPDH_0234 LQL 3 n/a PDH_0235 MEL 3 n/a PDH_0236 SLL 3 n/a PDH_0237 LVL 3n/a PDH_0238 MQL 3 n/a PDH_0239 AVL 3 n/a PDH_0240 AQL 3 n/a PDH_0241SQL 3 n/a PDH_0242 GQL 3 n/a PDH_0243 LEL 3 n/a PDH_0244 TVL 3 n/aPDH_0245 RQL 3 n/a PDH_0246 LLL 3 n/a PDH_0247 VQL 3 n/a PDH_0248 ALL 3n/a PDH_0249 AEL 3 n/a PDH_0250 WLL 3 n/a PDH_0251 WVL 3 n/a PDH_0252SEL 3 n/a PDH_0253 VLL 3 n/a PDH_0254 MVL 3 n/a PDH_0255 GEL 3 n/aPDH_0256 MLL 3 n/a PDH_0257 SVL 3 n/a PDH_0258 WQL 3 n/a PDH_0259 ISG 3n/a PDH_0260 DSG 3 n/a PDH_0261 VPG 3 n/a PDH_0262 VSG 3 n/a PDH_0263GAG 3 n/a PDH_0264 IPG 3 n/a PDH_0265 APG 3 n/a PDH_0266 TSG 3 n/aPDH_0267 DPG 3 n/a PDH_0268 LSG 3 n/a PDH_0269 LAG 3 n/a PDH_0270 NPG 3n/a PDH_0271 PAG 3 n/a PDH_0272 SAG 3 n/a PDH_0273 ASG 3 n/a PDH_0274RPG 3 n/a PDH_0275 HPG 3 n/a PDH_0276 GSG 3 n/a PDH_0277 GPG 3 n/aPDH_0278 IAG 3 n/a PDH_0279 LPG 3 n/a PDH_0280 AAG 3 n/a PDH_0281 TPG 3n/a PDH_0282 PSG 3 n/a PDH_0283 PPG 3 n/a PDH_0284 SPG 3 n/a PDH_0285RAG 3 n/a PDH_0286 HAG 3 n/a PDH_0287 SSG 3 n/a PDH_0288 HSG 3 n/aPDH_0289 RSG 3 n/a PDH_0290 TAG 3 n/a PDH_0291 DAG 3 n/a PDH_0292 NAG 3n/a PDH_0293 NSG 3 n/a PDH_0294 GTY 3 n/a PDH_0295 ITY 3 n/a PDH_0296LTY 3 n/a PDH_0297 ISY 3 n/a PDH_0298 GAY 3 n/a PDH_0299 LAY 3 n/aPDH_0300 HSY 3 n/a PDH_0301 AAY 3 n/a PDH_0302 ASY 3 n/a PDH_0303 TAY 3n/a PDH_0304 NAY 3 n/a PDH_0305 HTY 3 n/a PDH_0306 RTY 3 n/a PDH_0307PTY 3 n/a PDH_0308 RAY 3 n/a PDH_0309 ATY 3 n/a PDH_0310 STY 3 n/aPDH_0311 DSY 3 n/a PDH_0312 GSY 3 n/a PDH_0313 IAY 3 n/a PDH_0314 PAY 3n/a PDH_0315 VTY 3 n/a PDH_0316 PSY 3 n/a PDH_0317 TTY 3 n/a PDH_0318VAY 3 n/a PDH_0319 NTY 3 n/a PDH_0320 DAY 3 n/a PDH_0321 TSY 3 n/aPDH_0322 DTY 3 n/a PDH_0323 RSY 3 n/a PDH_0324 SSY 3 n/a PDH_0325 NSY 3n/a PDH_0326 SAY 3 n/a PDH_0327 HAY 3 n/a PDH_0328 LSY 3 n/a PDH_0329VSY 3 n/a PDH_0330 IVL 3 n/a PDH_0331 KWL 3 n/a PDH_0332 KVL 3 n/aPDH_0333 PLL 3 n/a PDH_0334 LGL 3 n/a PDH_0335 QWL 3 n/a PDH_0336 EGL 3n/a PDH_0337 EWL 3 n/a PDH_0338 EVL 3 n/a PDH_0339 QLL 3 n/a PDH_0340AGL 3 n/a PDH_0341 VWL 3 n/a PDH_0342 ELL 3 n/a PDH_0343 KGL 3 n/aPDH_0344 ILL 3 n/a PDH_0345 IGL 3 n/a PDH_0346 AWL 3 n/a PDH_0347 LWL 3n/a PDH_0348 QGL 3 n/a PDH_0349 PVL 3 n/a PDH_0350 VGL 3 n/a PDH_0351IWL 3 n/a PDH_0352 KLL 3 n/a PDH_0353 PGL 3 n/a PDH_0354 PWL 3 n/aPDH_0355 QVL 3 n/a PDH_0356 TGL 3 n/a PDH_0357 TWL 3 n/a PDH_0358 PAD 3n/a PDH_0359 PAL 3 n/a PDH_0360 PAA 3 n/a PDH_0361 PAH 3 n/a PDH_0362PAP 3 n/a PDH_0363 PAS 3 n/a PDH_0364 PAF 3 n/a PDH_0365 PAV 3 n/aPDH_0366 IQL 3 n/a PDH_0367 KRL 3 n/a PDH_0368 PRL 3 n/a PDH_0369 KQL 3n/a PDH_0370 QRL 3 n/a PDH_0371 KEL 3 n/a PDH_0372 EEL 3 n/a PDH_0373PEL 3 n/a PDH_0374 VRL 3 n/a PDH_0375 QEL 3 n/a PDH_0376 LRL 3 n/aPDH_0377 IEL 3 n/a PDH_0378 QQL 3 n/a PDH_0379 IRL 3 n/a PDH_0380 EQL 3n/a PDH_0381 ERL 3 n/a PDH_0382 TRL 3 n/a PDH_0383 ARL 3 n/a PDH_0384PQL 3 n/a PDH_0385 HSS 3 n/a PDH_0386 VST 3 n/a PDH_0387 HSA 3 n/aPDH_0388 YSG 3 n/a PDH_0389 ASS 3 n/a PDH_0390 HST 3 n/a PDH_0391 VSS 3n/a PDH_0392 YSA 3 n/a PDH_0393 DST 3 n/a PDH_0394 PST 3 n/a PDH_0395AST 3 n/a PDH_0396 FSS 3 n/a PDH_0397 LST 3 n/a PDH_0398 SST 3 n/aPDH_0399 FST 3 n/a PDH_0400 FSG 3 n/a PDH_0401 SSS 3 n/a PDH_0402 LSA 3n/a PDH_0403 LSS 3 n/a PDH_0404 PSA 3 n/a PDH_0405 DSA 3 n/a PDH_0406ASA 3 n/a PDH_0407 SSA 3 n/a PDH_0408 DSS 3 n/a PDH_0409 PSS 3 n/aPDH_0410 YSS 3 n/a PDH_0411 FSA 3 n/a PDH_0412 YST 3 n/a PDH_0413 VSA 3n/a PDH_0414 SGA 3 n/a PDH_0415 AGD 3 n/a PDH_0416 LGA 3 n/a PDH_0417SGY 3 n/a PDH_0418 SGD 3 n/a PDH_0419 FGY 3 n/a PDH_0420 DGY 3 n/aPDH_0421 LGS 3 n/a PDH_0422 FGS 3 n/a PDH_0423 DGS 3 n/a PDH_0424 YGS 3n/a PDH_0425 YGA 3 n/a PDH_0426 VGD 3 n/a PDH_0427 PGS 3 n/a PDH_0428VGY 3 n/a PDH_0429 VGS 3 n/a PDH_0430 VGA 3 n/a PDH_0431 LGD 3 n/aPDH_0432 AGY 3 n/a PDH_0433 LGY 3 n/a PDH_0434 HGD 3 n/a PDH_0435 HGA 3n/a PDH_0436 PGA 3 n/a PDH_0437 YGD 3 n/a PDH_0438 PGD 3 n/a PDH_0439YGY 3 n/a PDH_0440 PGY 3 n/a PDH_0441 SGS 3 n/a PDH_0442 HGY 3 n/aPDH_0443 FGD 3 n/a PDH_0444 FGA 3 n/a PDH_0445 AGS 3 n/a PDH_0446 DGD 3n/a PDH_0447 DGA 3 n/a PDH_0448 HGS 3 n/a PDH_0449 AGA 3 n/a PDH_0450SGV 3 n/a PDH_0451 LGV 3 n/a PDH_0452 AGG 3 n/a PDH_0453 SGG 3 n/aPDH_0454 DGV 3 n/a PDH_0455 PGV 3 n/a PDH_0456 HGV 3 n/a PDH_0457 YGV 3n/a PDH_0458 LGG 3 n/a PDH_0459 VGG 3 n/a PDH_0460 VGV 3 n/a PDH_0461FGV 3 n/a PDH_0462 PGG 3 n/a PDH_0463 YGG 3 n/a PDH_0464 HGG 3 n/aPDH_0465 DGG 3 n/a PDH_0466 AGV 3 n/a PDH_0467 FGG 3 n/a PDH_0468 HSD 3n/a PDH_0469 YSD 3 n/a PDH_0470 ASD 3 n/a PDH_0471 FSY 3 n/a PDH_0472FSD 3 n/a PDH_0473 SSD 3 n/a PDH_0474 VSD 3 n/a PDH_0475 PSD 3 n/aPDH_0476 LSD 3 n/a PDH_0477 YSY 3 n/a PDH_0478 DSD 3 n/a PDH_0479 SRL 3n/a PDH_0480 DQL 3 n/a PDH_0481 FRL 3 n/a PDH_0482 YRL 3 n/a PDH_0483HQL 3 n/a PDH_0484 NQL 3 n/a PDH_0485 NRL 3 n/a PDH_0486 FQL 3 n/aPDH_0487 DRL 3 n/a PDH_0488 HRL 3 n/a PDH_0489 YQL 3 n/a PDH_0490 VSW 3n/a PDH_0491 PSW 3 n/a PDH_0492 HSW 3 n/a PDH_0493 NSW 3 n/a PDH_0494FSW 3 n/a PDH_0495 ASW 3 n/a PDH_0496 TSW 3 n/a PDH_0497 LSW 3 n/aPDH_0498 DSW 3 n/a PDH_0499 ISW 3 n/a PDH_0500 SSW 3 n/a PDH_0501 YSW 3n/a PDH_0502 SGW 3 n/a PDH_0503 FGW 3 n/a PDH_0504 LGW 3 n/a PDH_0505AGW 3 n/a PDH_0506 VGW 3 n/a PDH_0507 YGW 3 n/a PDH_0508 PGW 3 n/aPDH_0509 DGW 3 n/a PDH_0510 HGW 3 n/a PDH_0511 IGD 3 n/a PDH_0512 GGA 3n/a PDH_0513 IGG 3 n/a PDH_0514 GGD 3 n/a PDH_0515 GGV 3 n/a PDH_0516RGD 3 n/a PDH_0517 TGV 3 n/a PDH_0518 RGV 3 n/a PDH_0519 GGG 3 n/aPDH_0520 IGA 3 n/a PDH_0521 IGV 3 n/a PDH_0522 RGG 3 n/a PDH_0523 RGA 3n/a PDH_0524 TGD 3 n/a PDH_0525 TGA 3 n/a PDH_0526 TGG 3 n/a PDH_0527NSA 3 n/a PDH_0528 NSD 3 n/a PDH_0529 TSD 3 n/a PDH_0530 TSA 3 n/aPDH_0531 ISA 3 n/a PDH_0532 ISD 3 n/a PDH_0533 HYD 3 n/a PDH_0534 HYG 3n/a PDH_0535 FYD 3 n/a PDH_0536 FYG 3 n/a PDH_0537 LYA 3 n/a PDH_0538LYD 3 n/a PDH_0539 VYA 3 n/a PDH_0540 VYD 3 n/a PDH_0541 TYA 3 n/aPDH_0542 LYG 3 n/a PDH_0543 DYD 3 n/a PDH_0544 HYA 3 n/a PDH_0545 TYD 3n/a PDH_0546 TYG 3 n/a PDH_0547 YYA 3 n/a PDH_0548 DYG 3 n/a PDH_0549YYD 3 n/a PDH_0550 NYG 3 n/a PDH_0551 NYD 3 n/a PDH_0552 PYG 3 n/aPDH_0553 YYG 3 n/a PDH_0554 PYD 3 n/a PDH_0555 NYA 3 n/a PDH_0556 FYA 3n/a PDH_0557 PYA 3 n/a PDH_0558 VYG 3 n/a PDH_0559 AYD 3 n/a PDH_0560IYG 3 n/a PDH_0561 AYA 3 n/a PDH_0562 SYG 3 n/a PDH_0563 IYD 3 n/aPDH_0564 IYA 3 n/a PDH_0565 AYG 3 n/a PDH_0566 DYA 3 n/a PDH_0567 SYD 3n/a PDH_0568 SYA 3 n/a PDH_0569 TSE 3 n/a PDH_0570 HSE 3 n/a PDH_0571YSE 3 n/a PDH_0572 ASE 3 n/a PDH_0573 NSE 3 n/a PDH_0574 FSE 3 n/aPDH_0575 DSE 3 n/a PDH_0576 ISE 3 n/a PDH_0577 SSE 3 n/a PDH_0578 VSE 3n/a PDH_0579 PSE 3 n/a PDH_0580 LSE 3 n/a PDH_0581 EWP 3 n/a PDH_0582PWP 3 n/a PDH_0583 KWA 3 n/a PDH_0584 IWP 3 n/a PDH_0585 LWA 3 n/aPDH_0586 LWV 3 n/a PDH_0587 AWV 3 n/a PDH_0588 AWA 3 n/a PDH_0589 PWA 3n/a PDH_0590 QWP 3 n/a PDH_0591 PWV 3 n/a PDH_0592 TWV 3 n/a PDH_0593TWP 3 n/a PDH_0594 QWA 3 n/a PDH_0595 KWP 3 n/a PDH_0596 QWV 3 n/aPDH_0597 EWV 3 n/a PDH_0598 VWA 3 n/a PDH_0599 AWP 3 n/a PDH_0600 VWV 3n/a PDH_0601 TWA 3 n/a PDH_0602 EWA 3 n/a PDH_0603 IWV 3 n/a PDH_0604VWP 3 n/a PDH_0605 IWA 3 n/a PDH_0606 LWP 3 n/a PDH_0607 KWV 3 n/aPDH_0608 HDY 3 n/a PDH_0609 IVY 3 n/a PDH_0610 PVY 3 n/a PDH_0611 AVY 3n/a PDH_0612 GVY 3 n/a PDH_0613 LVY 3 n/a PDH_0614 GDY 3 n/a PDH_0615ADY 3 n/a PDH_0616 VVY 3 n/a PDH_0617 NVY 3 n/a PDH_0618 SDY 3 n/aPDH_0619 RVY 3 n/a PDH_0620 LDY 3 n/a PDH_0621 HVY 3 n/a PDH_0622 PDY 3n/a PDH_0623 RDY 3 n/a PDH_0624 SVY 3 n/a PDH_0625 IDY 3 n/a PDH_0626DDY 3 n/a PDH_0627 NDY 3 n/a PDH_0628 VDY 3 n/a PDH_0629 DVY 3 n/aPDH_0630 TVY 3 n/a PDH_0631 TDY 3 n/a PDH_0632 GKL 3 n/a PDH_0633 GIL 3n/a PDH_0634 GRL 3 n/a PDH_0635 GGL 3 n/a PDH_0636 GAL 3 n/a PDH_0637GTL 3 n/a PDH_0638 LRG 3 n/a PDH_0639 DTG 3 n/a PDH_0640 ARG 3 n/aPDH_0641 YIG 3 n/a PDH_0642 ITG 3 n/a PDH_0643 PIG 3 n/a PDH_0644 DIG 3n/a PDH_0645 ATG 3 n/a PDH_0646 STG 3 n/a PDH_0647 HTG 3 n/a PDH_0648VRG 3 n/a PDH_0649 YRG 3 n/a PDH_0650 NIG 3 n/a PDH_0651 VIG 3 n/aPDH_0652 IRG 3 n/a PDH_0653 LTG 3 n/a PDH_0654 SRG 3 n/a PDH_0655 VTG 3n/a PDH_0656 AIG 3 n/a PDH_0657 IIG 3 n/a PDH_0658 FTG 3 n/a PDH_0659HIG 3 n/a PDH_0660 HRG 3 n/a PDH_0661 PTG 3 n/a PDH_0662 YTG 3 n/aPDH_0663 PRG 3 n/a PDH_0664 TIG 3 n/a PDH_0665 DRG 3 n/a PDH_0666 TRG 3n/a PDH_0667 FIG 3 n/a PDH_0668 NTG 3 n/a PDH_0669 FRG 3 n/a PDH_0670LIG 3 n/a PDH_0671 NRG 3 n/a PDH_0672 TTG 3 n/a PDH_0673 SIG 3 n/aPDH_0674 EAG 3 n/a PDH_0675 KAV 3 n/a PDH_0676 IAD 3 n/a PDH_0677 IAV 3n/a PDH_0678 KAD 3 n/a PDH_0679 QAA 3 n/a PDH_0680 LAA 3 n/a PDH_0681QAD 3 n/a PDH_0682 AAD 3 n/a PDH_0683 AAA 3 n/a PDH_0684 LAD 3 n/aPDH_0685 VAD 3 n/a PDH_0686 TAA 3 n/a PDH_0687 TAD 3 n/a PDH_0688 TAV 3n/a PDH_0689 EAA 3 n/a PDH_0690 AAV 3 n/a PDH_0691 QAV 3 n/a PDH_0692EAV 3 n/a PDH_0693 LAV 3 n/a PDH_0694 QAG 3 n/a PDH_0695 KAA 3 n/aPDH_0696 IAA 3 n/a PDH_0697 KAG 3 n/a PDH_0698 EAD 3 n/a PDH_0699 WVG 3n/a PDH_0700 VFG 3 n/a PDH_0701 SFG 3 n/a PDH_0702 RFG 3 n/a PDH_0703WAG 3 n/a PDH_0704 WFG 3 n/a PDH_0705 SVG 3 n/a PDH_0706 TVG 3 n/aPDH_0707 GFG 3 n/a PDH_0708 MVG 3 n/a PDH_0709 MFG 3 n/a PDH_0710 LVG 3n/a PDH_0711 WSG 3 n/a PDH_0712 AFG 3 n/a PDH_0713 MAG 3 n/a PDH_0714LFG 3 n/a PDH_0715 MSG 3 n/a PDH_0716 VVG 3 n/a PDH_0717 RVG 3 n/aPDH_0718 AVG 3 n/a PDH_0719 GVG 3 n/a PDH_0720 TFG 3 n/a PDH_0721 DGI 3n/a PDH_0722 LGI 3 n/a PDH_0723 SGI 3 n/a PDH_0724 HGI 3 n/a PDH_0725PGI 3 n/a PDH_0726 VGI 3 n/a PDH_0727 YGI 3 n/a PDH_0728 FGI 3 n/aPDH_0729 AGI 3 n/a PDH_0730 DVD 3 n/a PDH_0731 FVA 3 n/a PDH_0732 DVA 3n/a PDH_0733 YVD 3 n/a PDH_0734 YVA 3 n/a PDH_0735 DVG 3 n/a PDH_0736HVG 3 n/a PDH_0737 VVD 3 n/a PDH_0738 HVD 3 n/a PDH_0739 VVA 3 n/aPDH_0740 IVA 3 n/a PDH_0741 AVD 3 n/a PDH_0742 YVG 3 n/a PDH_0743 TVD 3n/a PDH_0744 FVG 3 n/a PDH_0745 FVD 3 n/a PDH_0746 TVA 3 n/a PDH_0747PVG 3 n/a PDH_0748 PVA 3 n/a PDH_0749 AVA 3 n/a PDH_0750 PVD 3 n/aPDH_0751 NVG 3 n/a PDH_0752 IVD 3 n/a PDH_0753 HVA 3 n/a PDH_0754 SVA 3n/a PDH_0755 SVD 3 n/a PDH_0756 IVG 3 n/a PDH_0757 NVA 3 n/a PDH_0758LVD 3 n/a PDH_0759 LVA 3 n/a PDH_0760 NVD 3 n/a PDH_0761 AYY 3 n/aPDH_0762 LFY 3 n/a PDH_0763 RFY 3 n/a PDH_0764 IFY 3 n/a PDH_0765 TYY 3n/a PDH_0766 RYY 3 n/a PDH_0767 PYY 3 n/a PDH_0768 VYY 3 n/a PDH_0769SFY 3 n/a PDH_0770 GYY 3 n/a PDH_0771 GFY 3 n/a PDH_0772 DFY 3 n/aPDH_0773 VFY 3 n/a PDH_0774 HYY 3 n/a PDH_0775 SYY 3 n/a PDH_0776 PFY 3n/a PDH_0777 LYY 3 n/a PDH_0778 IYY 3 n/a PDH_0779 TFY 3 n/a PDH_0780NFY 3 n/a PDH_0781 HFY 3 n/a PDH_0782 AFY 3 n/a PDH_0783 DYY 3 n/aPDH_0784 NYY 3 n/a PDH_0785 VVV 3 n/a PDH_0786 VVF 3 n/a PDH_0787 VVP 3n/a PDH_0788 VVH 3 n/a PDH_0789 VVS 3 n/a PDH_0790 GDL 3 n/a PDH_0791SAL 3 n/a PDH_0792 RAL 3 n/a PDH_0793 RGL 3 n/a PDH_0794 IAL 3 n/aPDH_0795 LDL 3 n/a PDH_0796 TDL 3 n/a PDH_0797 ADL 3 n/a PDH_0798 VDL 3n/a PDH_0799 IDL 3 n/a PDH_0800 SDL 3 n/a PDH_0801 TAL 3 n/a PDH_0802RDL 3 n/a PDH_0803 AAL 3 n/a PDH_0804 SGL 3 n/a PDH_0805 PDL 3 n/aPDH_0806 LAL 3 n/a PDH_0807 AGE 3 n/a PDH_0808 SGE 3 n/a PDH_0809 HGE 3n/a PDH_0810 LGE 3 n/a PDH_0811 VGE 3 n/a PDH_0812 PGE 3 n/a PDH_0813YGE 3 n/a PDH_0814 DGE 3 n/a PDH_0815 FGE 3 n/a PDH_0816 SDG 3 n/aPDH_0817 NDG 3 n/a PDH_0818 GDG 3 n/a PDH_0819 HDG 3 n/a PDH_0820 ADG 3n/a PDH_0821 TDG 3 n/a PDH_0822 IDG 3 n/a PDH_0823 DDG 3 n/a PDH_0824VDG 3 n/a PDH_0825 RDG 3 n/a PDH_0826 LDG 3 n/a PDH_0827 PDG 3 n/aPDH_0828 RHG 3 n/a PDH_0829 RLG 3 n/a PDH_0830 RYG 3 n/a PDH_0831 DLY 3n/a PDH_0832 IWY 3 n/a PDH_0833 LWY 3 n/a PDH_0834 ALY 3 n/a PDH_0835RWY 3 n/a PDH_0836 SLY 3 n/a PDH_0837 HLY 3 n/a PDH_0838 ILY 3 n/aPDH_0839 SWY 3 n/a PDH_0840 GLY 3 n/a PDH_0841 RLY 3 n/a PDH_0842 DWY 3n/a PDH_0843 NLY 3 n/a PDH_0844 VWY 3 n/a PDH_0845 GWY 3 n/a PDH_0846AWY 3 n/a PDH_0847 HWY 3 n/a PDH_0848 PLY 3 n/a PDH_0849 LLY 3 n/aPDH_0850 TWY 3 n/a PDH_0851 TLY 3 n/a PDH_0852 NWY 3 n/a PDH_0853 VLY 3n/a PDH_0854 PWY 3 n/a PDH_0855 GSL 3 n/a PDH_0856 ISL 3 n/a PDH_0857DWL 3 n/a PDH_0858 SSL 3 n/a PDH_0859 TSL 3 n/a PDH_0860 VSL 3 n/aPDH_0861 DSL 3 n/a PDH_0862 HWL 3 n/a PDH_0863 ASL 3 n/a PDH_0864 SWL 3n/a PDH_0865 NWL 3 n/a PDH_0866 NLL 3 n/a PDH_0867 DLL 3 n/a PDH_0868RSL 3 n/a PDH_0869 PSL 3 n/a PDH_0870 HLL 3 n/a PDH_0871 GWL 3 n/aPDH_0872 HSL 3 n/a PDH_0873 NSL 3 n/a PDH_0874 LSL 3 n/a PDH_0875 RWL 3n/a PDH_0876 FDY 3 n/a PDH_0877 YYY 3 n/a PDH_0878 FYY 3 n/a PDH_0879YDY 3 n/a PDH_0880 LIL 3 n/a PDH_0881 LKL 3 n/a PDH_0882 LTL 3 n/aPDH_0883 YAG 3 n/a PDH_0884 FPG 3 n/a PDH_0885 YPG 3 n/a PDH_0886 FAG 3n/a PDH_0887 FYH 3 n/a PDH_0888 LYH 3 n/a PDH_0889 IYH 3 n/a PDH_0890SYH 3 n/a PDH_0891 TYH 3 n/a PDH_0892 YYH 3 n/a PDH_0893 NYH 3 n/aPDH_0894 PYH 3 n/a PDH_0895 AYH 3 n/a PDH_0896 VYH 3 n/a PDH_0897 HYH 3n/a PDH_0898 DYH 3 n/a PDH_0899 YTAM 4 5639 PDH_0900 HTAI 4 5640PDH_0901 YTAS 4 5641 PDH_0902 YTAI 4 5642 PDH_0903 YTAR 4 5643 PDH_0904PTAS 4 5644 PDH_0905 LTAM 4 5645 PDH_0906 DTAI 4 5646 PDH_0907 FTAS 45647 PDH_0908 FTAM 4 5648 PDH_0909 LTAS 4 5649 PDH_0910 ATAI 4 5650PDH_0911 STAI 4 5651 PDH_0912 FTAR 4 5652 PDH_0913 DTAM 4 4420 PDH_0914STAR 4 5653 PDH_0915 LTAR 4 5654 PDH_0916 FTAI 4 5655 PDH_0917 LTAI 45656 PDH_0918 STAM 4 5657 PDH_0919 ATAM 4 5658 PDH_0920 STAS 4 5659PDH_0921 ATAR 4 5660 PDH_0922 HTAS 4 5661 PDH_0923 HTAM 4 5662 PDH_0924VTAI 4 3907 PDH_0925 DTAR 4 5663 PDH_0926 HTAR 4 5664 PDH_0927 ATAS 45665 PDH_0928 VTAM 4 5666 PDH_0929 PTAR 4 5667 PDH_0930 DTAS 4 5668PDH_0931 VTAS 4 5669 PDH_0932 PTAM 4 5670 PDH_0933 VTAR 4 5671 PDH_0934PTAI 4 5672 PDH_0935 LVAT 4 5673 PDH_0936 LAAT 4 5674 PDH_0937 MVAT 45675 PDH_0938 TGAT 4 5676 PDH_0939 AVAT 4 5677 PDH_0940 VAAT 4 3803PDH_0941 PAAT 4 5678 PDH_0942 KGAT 4 5679 PDH_0943 EGAT 4 5680 PDH_0944PVAT 4 5681 PDH_0945 AGAT 4 5682 PDH_0946 QAAT 4 5683 PDH_0947 AAAT 45684 PDH_0948 VVAT 4 5685 PDH_0949 VGAT 4 3756 PDH_0950 TVAT 4 5686PDH_0951 EVAT 4 5687 PDH_0952 LGAT 4 5688 PDH_0953 KAAT 4 5689 PDH_0954MGAT 4 5690 PDH_0955 PGAT 4 5691 PDH_0956 QVAT 4 5692 PDH_0957 KVAT 45693 PDH_0958 EAAT 4 5694 PDH_0959 TAAT 4 5695 PDH_0960 MAAT 4 5696PDH_0961 QGAT 4 5697 PDH_0962 RFGA 4 5698 PDH_0963 MFGE 4 5699 PDH_0964MFGA 4 5700 PDH_0965 VFGG 4 5701 PDH_0966 RFGE 4 5702 PDH_0967 MFGV 45703 PDH_0968 VFGA 4 5704 PDH_0969 VFGE 4 5705 PDH_0970 VFGV 4 5706PDH_0971 MFGG 4 5707 PDH_0972 LFGV 4 5708 PDH_0973 SFGE 4 5709 PDH_0974SFGA 4 5710 PDH_0975 RFGG 4 5711 PDH_0976 SFGV 4 5712 PDH_0977 LFGA 45713 PDH_0978 SFGG 4 5714 PDH_0979 LFGG 4 5715 PDH_0980 LFGE 4 5716PDH_0981 WFGE 4 3966 PDH_0982 WFGG 4 5717 PDH_0983 WFGV 4 5718 PDH_0984WFGA 4 5719 PDH_0985 TFGG 4 4154 PDH_0986 TFGE 4 5720 PDH_0987 AFGV 45721 PDH_0988 AFGA 4 5722 PDH_0989 GFGV 4 5723 PDH_0990 GFGA 4 5724PDH_0991 GFGG 4 5725 PDH_0992 TFGV 4 5726 PDH_0993 AFGE 4 5727 PDH_0994TFGA 4 5728 PDH_0995 RFGV 4 5729 PDH_0996 AFGG 4 5730 PDH_0997 GFGE 45731 PDH_0998 AGDY 4 5732 PDH_0999 PGYY 4 5733 PDH_1000 VGAY 4 5734PDH_1001 HGSY 4 5735 PDH_1002 SGSY 4 3763 PDH_1003 PGDY 4 5736 PDH_1004LGDY 4 5737 PDH_1005 DGAY 4 5738 PDH_1006 FGDY 4 5739 PDH_1007 LGAY 45740 PDH_1008 DGYY 4 5741 PDH_1009 VGSY 4 5742 PDH_1010 YGAY 4 5743PDH_1011 FGYY 4 5744 PDH_1012 DGDY 4 5745 PDH_1013 AGYY 4 5746 PDH_1014YGSY 4 5747 PDH_1015 VGYY 4 5748 PDH_1016 AGAY 4 5749 PDH_1017 DGSY 45750 PDH_1018 HGDY 4 5751 PDH_1019 FGAY 4 5752 PDH_1020 HGYY 4 5753PDH_1021 YGYY 4 5754 PDH_1022 SGYY 4 4187 PDH_1023 SGAY 4 5755 PDH_1024AGSY 4 5756 PDH_1025 HGAY 4 5757 PDH_1026 PGAY 4 5758 PDH_1027 PGSY 45759 PDH_1028 LGSY 4 5760 PDH_1029 VGDY 4 5761 PDH_1030 SGDY 4 5762PDH_1031 LGYY 4 5763 PDH_1032 FGSY 4 5764 PDH_1033 YGDY 4 4350 PDH_1034YSSV 4 5765 PDH_1035 YSSI 4 5766 PDH_1036 YSSS 4 4442 PDH_1037 YSSR 45767 PDH_1038 YSSP 4 5768 PDH_1039 YSSA 4 5769 PDH_1040 YSSL 4 5770PDH_1041 YSSG 4 4462 PDH_1042 YSST 4 5771 PDH_1043 EVRG 4 5772 PDH_1044PVRG 4 5773 PDH_1045 PVTG 4 5774 PDH_1046 EVAG 4 5775 PDH_1047 MVTG 45776 PDH_1048 MVGG 4 5777 PDH_1049 EVGG 4 3728 PDH_1050 QVRG 4 5778PDH_1051 MVRG 4 4017 PDH_1052 QVGG 4 5779 PDH_1053 VVAG 4 5780 PDH_1054EVTG 4 5781 PDH_1055 VVRG 4 5782 PDH_1056 PVAG 4 5783 PDH_1057 LVAG 45784 PDH_1058 LVRG 4 5785 PDH_1059 QVTG 4 5786 PDH_1060 PVGG 4 5787PDH_1061 AVGG 4 5788 PDH_1062 TVGG 4 5789 PDH_1063 KVGG 4 5790 PDH_1064TVAG 4 5791 PDH_1065 AVTG 4 5792 PDH_1066 KVRG 4 5793 PDH_1067 LVTG 45794 PDH_1068 AVRG 4 5795 PDH_1069 LVGG 4 5796 PDH_1070 AVAG 4 4473PDH_1071 QVAG 4 5797 PDH_1072 KVTG 4 5798 PDH_1073 TVTG 4 5799 PDH_1074VVGG 4 5800 PDH_1075 KVAG 4 5801 PDH_1076 MVAG 4 5802 PDH_1077 VVTG 45803 PDH_1078 TVRG 4 5804 PDH_1079 SDGY 4 5805 PDH_1080 IDGF 4 5806PDH_1081 ADGY 4 5807 PDH_1082 ADGS 4 5808 PDH_1083 RDGF 4 5809 PDH_1084IDGS 4 5810 PDH_1085 GDGS 4 5811 PDH_1086 LDGY 4 5812 PDH_1087 GDGY 45813 PDH_1088 IDGY 4 5814 PDH_1089 SDGS 4 5815 PDH_1090 SDGF 4 5816PDH_1091 VDGF 4 5817 PDH_1092 GDGF 4 5818 PDH_1093 TDGY 4 5819 PDH_1094RDGY 4 4410 PDH_1095 VDGY 4 5820 PDH_1096 TDGS 4 5821 PDH_1097 RDGS 45822 PDH_1098 LDGF 4 5823 PDH_1099 VDGS 4 5824 PDH_1100 ADGF 4 5825PDH_1101 LDGS 4 5826 PDH_1102 PDGS 4 5827 PDH_1103 PDGF 4 5828 PDH_1104PDGY 4 5829 PDH_1105 TDGF 4 5830 PDH_1106 NYGG 4 5831 PDH_1107 TYGD 45832 PDH_1108 LYGD 4 5833 PDH_1109 FYGG 4 5834 PDH_1110 SYGG 4 5835PDH_1111 TYGG 4 5836 PDH_1112 LYGA 4 5837 PDH_1113 SYGA 4 5838 PDH_1114LYGG 4 5839 PDH_1115 VYGD 4 5840 PDH_1116 SYGD 4 5841 PDH_1117 AYGG 45842 PDH_1118 VYGG 4 5843 PDH_1119 HYGG 4 5844 PDH_1120 FYGA 4 5845PDH_1121 NYGD 4 5846 PDH_1122 TYGA 4 5847 PDH_1123 FYGD 4 5848 PDH_1124IYGD 4 5849 PDH_1125 DYGD 4 4349 PDH_1126 PYGD 4 5850 PDH_1127 DYGA 45851 PDH_1128 HYGA 4 5852 PDH_1129 PYGA 4 5853 PDH_1130 PYGG 4 5854PDH_1131 HYGD 4 5855 PDH_1132 AYGA 4 5856 PDH_1133 VYGA 4 5857 PDH_1134YYGD 4 5858 PDH_1135 AYGD 4 5859 PDH_1136 NYGA 4 5860 PDH_1137 YYGA 45861 PDH_1138 YYGG 4 5862 PDH_1139 IYGG 4 5863 PDH_1140 IYGA 4 5864PDH_1141 DYGG 4 4357 PDH_1142 LMAT 4 5865 PDH_1143 VTAT 4 5866 PDH_1144KMAT 4 5867 PDH_1145 QMAT 4 5868 PDH_1146 ETAT 4 5869 PDH_1147 TTAT 45870 PDH_1148 TMAT 4 5871 PDH_1149 PTAT 4 5872 PDH_1150 VMAT 4 5873PDH_1151 LTAT 4 5874 PDH_1152 KTAT 4 5875 PDH_1153 MMAT 4 5876 PDH_1154ATAT 4 5877 PDH_1155 QTAT 4 5878 PDH_1156 PMAT 4 5879 PDH_1157 MTAT 45880 PDH_1158 EMAT 4 4402 PDH_1159 AMAT 4 5881 PDH_1160 TYSA 4 5882PDH_1161 LYSS 4 5883 PDH_1162 LYST 4 5884 PDH_1163 QYSS 4 5885 PDH_1164VYST 4 5886 PDH_1165 VYSS 4 5887 PDH_1166 AYSA 4 5888 PDH_1167 PYSG 45889 PDH_1168 PYST 4 5890 PDH_1169 VYSA 4 5891 PDH_1170 PYSS 4 5892PDH_1171 VYSG 4 5893 PDH_1172 PYSA 4 5894 PDH_1173 KYST 4 5895 PDH_1174QYST 4 5896 PDH_1175 TYSG 4 5897 PDH_1176 TYST 4 5898 PDH_1177 QYSA 45899 PDH_1178 AYSS 4 5900 PDH_1179 TYSS 4 5901 PDH_1180 IYSA 4 5902PDH_1181 AYST 4 5903 PDH_1182 IYSG 4 5904 PDH_1183 EYSS 4 4479 PDH_1184KYSG 4 5905 PDH_1185 EYSA 4 5906 PDH_1186 LYSG 4 5907 PDH_1187 AYSG 45908 PDH_1188 EYSG 4 5909 PDH_1189 LYSA 4 5910 PDH_1190 QYSG 4 5911PDH_1191 IYST 4 5912 PDH_1192 EYST 4 5913 PDH_1193 KYSS 4 5914 PDH_1194IYSS 4 5915 PDH_1195 KYSA 4 5916 PDH_1196 QWLS 4 5917 PDH_1197 QWLL 45918 PDH_1198 QWLP 4 5919 PDH_1199 QWLD 4 5920 PDH_1200 QWLY 4 5921PDH_1201 QWLA 4 5922 PDH_1202 QWLV 4 4475 PDH_1203 QWLH 4 5923 PDH_1204QWLF 4 5924 PDH_1205 PVAD 4 5925 PDH_1206 PVAA 4 5926 PDH_1207 IVAA 45927 PDH_1208 EVAA 4 5928 PDH_1209 EVAV 4 5929 PDH_1210 VVAA 4 3802PDH_1211 IVAD 4 5930 PDH_1212 EVAD 4 5931 PDH_1213 IVAG 4 5932 PDH_1214QVAD 4 5933 PDH_1215 AVAA 4 5934 PDH_1216 AVAV 4 5935 PDH_1217 AVAD 45936 PDH_1218 KVAA 4 5937 PDH_1219 QVAA 4 5938 PDH_1220 TVAV 4 5939PDH_1221 LVAD 4 5940 PDH_1222 LVAA 4 5941 PDH_1223 IVAV 4 5942 PDH_1224VVAD 4 5943 PDH_1225 VVAV 4 5944 PDH_1226 QVAV 4 5945 PDH_1227 PVAV 45946 PDH_1228 KVAV 4 5947 PDH_1229 LVAV 4 5948 PDH_1230 TVAD 4 5949PDH_1231 KVAD 4 5950 PDH_1232 TVAA 4 5951 PDH_1233 STVA 4 5952 PDH_1234STVK 4 5953 PDH_1235 RTVA 4 5954 PDH_1236 ITVT 4 5955 PDH_1237 PTVA 45956 PDH_1238 ATVT 4 5957 PDH_1239 ATVK 4 5958 PDH_1240 VTVK 4 5959PDH_1241 TTVK 4 5960 PDH_1242 PTVE 4 5961 PDH_1243 VTVT 4 5962 PDH_1244STVT 4 5963 PDH_1245 VTVE 4 5964 PDH_1246 TTVT 4 4352 PDH_1247 LTVA 45965 PDH_1248 RTVT 4 5966 PDH_1249 LTVE 4 5967 PDH_1250 TTVE 4 5968PDH_1251 RTVK 4 5969 PDH_1252 VTVA 4 5970 PDH_1253 STVE 4 5971 PDH_1254ATVA 4 5972 PDH_1255 GTVE 4 5973 PDH_1256 GTVA 4 5974 PDH_1257 ITVE 45975 PDH_1258 PTVT 4 5976 PDH_1259 ITVA 4 5977 PDH_1260 ATVE 4 5978PDH_1261 GTVK 4 5979 PDH_1262 LTVK 4 5980 PDH_1263 ITVK 4 5981 PDH_1264RTVE 4 5982 PDH_1265 LTVT 4 5983 PDH_1266 TTVA 4 5984 PDH_1267 PTVK 45985 PDH_1268 GTVT 4 5986 PDH_1269 SSSA 4 5987 PDH_1270 SSSS 4 4480PDH_1271 SSSL 4 5988 PDH_1272 SSSW 4 4443 PDH_1273 SSSR 4 5989 PDH_1274SSSV 4 5990 PDH_1275 SSST 4 5991 PDH_1276 SSSM 4 5992 PDH_1277 SSSG 45993 PDH_1278 LSYG 4 5994 PDH_1279 PYYG 4 5995 PDH_1280 ASYG 4 5996PDH_1281 FYYG 4 5997 PDH_1282 DSYG 4 5998 PDH_1283 VYYG 4 5999 PDH_1284IYYG 4 6000 PDH_1285 DYYG 4 6001 PDH_1286 HYYG 4 6002 PDH_1287 SYYG 46003 PDH_1288 YYYG 4 3989 PDH_1289 VSYG 4 6004 PDH_1290 NSYG 4 6005PDH_1291 SSYG 4 6006 PDH_1292 FSYG 4 6007 PDH_1293 ISYG 4 6008 PDH_1294TSYG 4 6009 PDH_1295 LYYG 4 6010 PDH_1296 PSYG 4 6011 PDH_1297 AYYG 46012 PDH_1298 YSYG 4 4433 PDH_1299 HSYG 4 6013 PDH_1300 NYYG 4 6014PDH_1301 TYYG 4 6015 PDH_1302 FSWY 4 6016 PDH_1303 SSWF 4 6017 PDH_1304DSWS 4 6018 PDH_1305 LSWS 4 6019 PDH_1306 DSWY 4 6020 PDH_1307 LSWF 46021 PDH_1308 LSWY 4 6022 PDH_1309 VSWS 4 6023 PDH_1310 HSWY 4 6024PDH_1311 SSWS 4 6025 PDH_1312 PSWS 4 6026 PDH_1313 SSWY 4 4444 PDH_1314FSWF 4 6027 PDH_1315 FSWS 4 6028 PDH_1316 PSWF 4 6029 PDH_1317 VSWF 46030 PDH_1318 HSWF 4 6031 PDH_1319 VSWY 4 6032 PDH_1320 HSWS 4 6033PDH_1321 DSWF 4 6034 PDH_1322 PSWY 4 6035 PDH_1323 ASWY 4 6036 PDH_1324YSWS 4 6037 PDH_1325 ASWF 4 6038 PDH_1326 ASWS 4 6039 PDH_1327 YSWF 46040 PDH_1328 YSWY 4 6041 PDH_1329 IQLV 4 6042 PDH_1330 AQLG 4 6043PDH_1331 IQLA 4 6044 PDH_1332 EQLV 4 6045 PDH_1333 AQLA 4 6046 PDH_1334IQLG 4 6047 PDH_1335 KQLD 4 6048 PDH_1336 TQLV 4 6049 PDH_1337 QQLA 46050 PDH_1338 AQLD 4 6051 PDH_1339 IQLD 4 6052 PDH_1340 AQLV 4 6053PDH_1341 KQLA 4 6054 PDH_1342 KQLG 4 6055 PDH_1343 LQLD 4 6056 PDH_1344LQLG 4 6057 PDH_1345 TQLA 4 6058 PDH_1346 VQLD 4 6059 PDH_1347 TQLD 46060 PDH_1348 VQLA 4 6061 PDH_1349 EQLD 4 6062 PDH_1350 VQLG 4 6063PDH_1351 TQLG 4 6064 PDH_1352 PQLD 4 6065 PDH_1353 QQLV 4 4455 PDH_1354QQLD 4 6066 PDH_1355 PQLA 4 6067 PDH_1356 PQLG 4 6068 PDH_1357 VQLV 46069 PDH_1358 QQLG 4 6070 PDH_1359 KQLV 4 6071 PDH_1360 LQLV 4 6072PDH_1361 LQLA 4 6073 PDH_1362 EQLA 4 6074 PDH_1363 PQLV 4 6075 PDH_1364EQLG 4 6076 PDH_1365 DGSA 4 6077 PDH_1366 DGSS 4 6078 PDH_1367 SGSA 46079 PDH_1368 DGSD 4 6080 PDH_1369 SGSD 4 6081 PDH_1370 PGSD 4 6082PDH_1371 FGSS 4 6083 PDH_1372 HGSA 4 6084 PDH_1373 YGSS 4 6085 PDH_1374FGSA 4 6086 PDH_1375 FGSD 4 6087 PDH_1376 LGSA 4 6088 PDH_1377 LGSS 46089 PDH_1378 AGSD 4 6090 PDH_1379 VGSS 4 6091 PDH_1380 AGSS 4 6092PDH_1381 HGSD 4 6093 PDH_1382 VGSA 4 6094 PDH_1383 YGSA 4 6095 PDH_1384YGSD 4 6096 PDH_1385 AGSA 4 6097 PDH_1386 HGSS 4 6098 PDH_1387 VGSD 46099 PDH_1388 PGSA 4 6100 PDH_1389 PGSS 4 6101 PDH_1390 SGSS 4 6102PDH_1391 LGSD 4 6103 PDH_1392 SGWY 4 4464 PDH_1393 PGWY 4 6104 PDH_1394FGWY 4 6105 PDH_1395 YGWY 4 6106 PDH_1396 AGWY 4 6107 PDH_1397 VGWY 46108 PDH_1398 LGWY 4 6109 PDH_1399 DGWY 4 6110 PDH_1400 HGWY 4 6111PDH_1401 PSGW 4 6112 PDH_1402 YSGW 4 6113 PDH_1403 FSGR 4 6114 PDH_1404VSGG 4 6115 PDH_1405 NSGR 4 6116 PDH_1406 DSGR 4 6117 PDH_1407 ASGR 46118 PDH_1408 FSGW 4 6119 PDH_1409 DSGW 4 6120 PDH_1410 VSGR 4 6121PDH_1411 ISGW 4 6122 PDH_1412 LSGW 4 6123 PDH_1413 DSGG 4 3723 PDH_1414HSGR 4 6124 PDH_1415 NSGW 4 6125 PDH_1416 HSGG 4 6126 PDH_1417 HSGW 46127 PDH_1418 ISGG 4 6128 PDH_1419 YSGR 4 6129 PDH_1420 ISGR 4 6130PDH_1421 YSGG 4 6131 PDH_1422 NSGG 4 6132 PDH_1423 SSGW 4 4463 PDH_1424VSGW 4 6133 PDH_1425 SSGR 4 6134 PDH_1426 LSGR 4 6135 PDH_1427 PSGR 46136 PDH_1428 FSGG 4 6137 PDH_1429 TSGR 4 6138 PDH_1430 TSGW 4 6139PDH_1431 ASGG 4 6140 PDH_1432 LSGG 4 6141 PDH_1433 ASGW 4 6142 PDH_1434PSGG 4 6143 PDH_1435 TSGG 4 6144 PDH_1436 SSGG 4 6145 PDH_1437 VGYD 46146 PDH_1438 AGYD 4 6147 PDH_1439 DGYD 4 6148 PDH_1440 AGYA 4 6149PDH_1441 DGYA 4 6150 PDH_1442 FGYA 4 6151 PDH_1443 FGYD 4 6152 PDH_1444PGYA 4 6153 PDH_1445 DGYS 4 6154 PDH_1446 YGYA 4 6155 PDH_1447 FGYS 46156 PDH_1448 VGYA 4 6157 PDH_1449 PGYD 4 6158 PDH_1450 PGYS 4 6159PDH_1451 VGYS 4 6160 PDH_1452 YGYD 4 6161 PDH_1453 HGYA 4 6162 PDH_1454YGYS 4 6163 PDH_1455 HGYD 4 6164 PDH_1456 SGYS 4 6165 PDH_1457 LGYA 46166 PDH_1458 HGYS 4 6167 PDH_1459 LGYD 4 6168 PDH_1460 AGYS 4 6169PDH_1461 LGYS 4 6170 PDH_1462 SGYD 4 4393 PDH_1463 SGYA 4 6171 PDH_1464QVTA 4 6172 PDH_1465 PVTA 4 6173 PDH_1466 LVTT 4 6174 PDH_1467 PVTT 46175 PDH_1468 PVTE 4 6176 PDH_1469 QVTT 4 6177 PDH_1470 AVTA 4 6178PDH_1471 QVTK 4 6179 PDH_1472 IVTA 4 6180 PDH_1473 PVTK 4 6181 PDH_1474LVTK 4 6182 PDH_1475 AVTE 4 6183 PDH_1476 LVTA 4 6184 PDH_1477 EVTA 46185 PDH_1478 LVTE 4 6186 PDH_1479 EVTE 4 6187 PDH_1480 IVTE 4 6188PDH_1481 VVTA 4 3906 PDH_1482 TVTK 4 6189 PDH_1483 TVTT 4 4353 PDH_1484IVTT 4 6190 PDH_1485 VVTE 4 6191 PDH_1486 IVTK 4 6192 PDH_1487 TVTE 46193 PDH_1488 AVTT 4 6194 PDH_1489 KVTA 4 6195 PDH_1490 KVTE 4 6196PDH_1491 AVTK 4 6197 PDH_1492 EVTK 4 6198 PDH_1493 KVTK 4 6199 PDH_1494VVTK 4 6200 PDH_1495 TVTA 4 6201 PDH_1496 QVTE 4 6202 PDH_1497 VVTT 46203 PDH_1498 EVTT 4 6204 PDH_1499 KVTT 4 6205 PDH_1500 AAAG 4 4453PDH_1501 AAAS 4 6206 PDH_1502 AAAL 4 6207 PDH_1503 AAAR 4 6208 PDH_1504AAAI 4 6209 PDH_1505 AAAV 4 6210 PDH_1506 AAAP 4 6211 PDH_1507 AAAA 46212 PDH_1508 AIFG 4 6213 PDH_1509 ATFG 4 6214 PDH_1510 PTFG 4 6215PDH_1511 TTFG 4 6216 PDH_1512 ITFG 4 4153 PDH_1513 RTFG 4 6217 PDH_1514STFG 4 6218 PDH_1515 SIFG 4 6219 PDH_1516 LIFG 4 6220 PDH_1517 RIFG 46221 PDH_1518 TIFG 4 4291 PDH_1519 GIFG 4 6222 PDH_1520 IIFG 4 6223PDH_1521 LTFG 4 6224 PDH_1522 VIFG 4 6225 PDH_1523 PIFG 4 6226 PDH_1524GTFG 4 6227 PDH_1525 VTFG 4 6228 PDH_1526 VAAK 4 6229 PDH_1527 VAAL 46230 PDH_1528 VAAP 4 6231 PDH_1529 VAAQ 4 6232 PDH_1530 VAAA 4 6233PDH_1531 VAAE 4 6234 PDH_1532 VAAV 4 6235 PDH_1533 VAAI 4 6236 PDH_1534YYYD 4 4182 PDH_1535 VYYA 4 6237 PDH_1536 AYYD 4 6238 PDH_1537 YYYA 46239 PDH_1538 PYYD 4 6240 PDH_1539 IYYD 4 6241 PDH_1540 VYYD 4 6242PDH_1541 IYYA 4 6243 PDH_1542 TYYA 4 6244 PDH_1543 LYYD 4 6245 PDH_1544DYYD 4 6246 PDH_1545 SYYD 4 6247 PDH_1546 LYYA 4 6248 PDH_1547 HYYA 46249 PDH_1548 DYYA 4 6250 PDH_1549 SYYA 4 6251 PDH_1550 FYYD 4 6252PDH_1551 FYYA 4 6253 PDH_1552 PYYA 4 6254 PDH_1553 AYYA 4 6255 PDH_1554HYYD 4 6256 PDH_1555 NYYA 4 6257 PDH_1556 TYYD 4 6258 PDH_1557 NYYD 46259 PDH_1558 GYGY 4 6260 PDH_1559 LYGY 4 6261 PDH_1560 SSGY 4 4186PDH_1561 RYGY 4 6262 PDH_1562 TYGY 4 6263 PDH_1563 TSGY 4 6264 PDH_1564VYGY 4 6265 PDH_1565 ISGY 4 6266 PDH_1566 ASGY 4 6267 PDH_1567 PSGY 46268 PDH_1568 RSGY 4 6269 PDH_1569 GAGY 4 6270 PDH_1570 AAGY 4 6271PDH_1571 LSGY 4 6272 PDH_1572 SYGY 4 4434 PDH_1573 VSGY 4 6273 PDH_1574VAGY 4 6274 PDH_1575 TAGY 4 6275 PDH_1576 PAGY 4 6276 PDH_1577 SAGY 46277 PDH_1578 RAGY 4 6278 PDH_1579 IAGY 4 6279 PDH_1580 AYGY 4 6280PDH_1581 PYGY 4 6281 PDH_1582 LAGY 4 6282 PDH_1583 IYGY 4 6283 PDH_1584GSGY 4 6284 PDH_1585 LQLL 4 6285 PDH_1586 AQLW 4 6286 PDH_1587 TQLW 46287 PDH_1588 QQLL 4 6288 PDH_1589 IQLW 4 4427 PDH_1590 EQLL 4 6289PDH_1591 QQLW 4 6290 PDH_1592 IQLL 4 6291 PDH_1593 EQLW 4 6292 PDH_1594VQLW 4 6293 PDH_1595 KQLW 4 6294 PDH_1596 KQLL 4 6295 PDH_1597 PQLW 46296 PDH_1598 VQLL 4 6297 PDH_1599 TQLL 4 6298 PDH_1600 PQLL 4 6299PDH_1601 LQLW 4 6300 PDH_1602 AQLL 4 6301 PDH_1603 LGVA 4 6302 PDH_1604LGGA 4 6303 PDH_1605 KGIA 4 6304 PDH_1606 KGSA 4 6305 PDH_1607 VGIA 46306 PDH_1608 KGGA 4 6307 PDH_1609 MGGA 4 6308 PDH_1610 TGGA 4 6309PDH_1611 QGSA 4 6310 PDH_1612 QGIA 4 6311 PDH_1613 EGSA 4 6312 PDH_1614PGVA 4 6313 PDH_1615 QGVA 4 6314 PDH_1616 EGIA 4 6315 PDH_1617 AGVA 46316 PDH_1618 MGVA 4 6317 PDH_1619 EGGA 4 3671 PDH_1620 PGIA 4 6318PDH_1621 AGGA 4 6319 PDH_1622 VGGA 4 6320 PDH_1623 EGVA 4 6321 PDH_1624PGGA 4 6322 PDH_1625 QGGA 4 6323 PDH_1626 AGIA 4 6324 PDH_1627 LGIA 46325 PDH_1628 MGIA 4 6326 PDH_1629 TGSA 4 6327 PDH_1630 TGIA 4 6328PDH_1631 MGSA 4 6329 PDH_1632 KGVA 4 6330 PDH_1633 TGVA 4 6331 PDH_1634VGVA 4 6332 PDH_1635 PFGE 4 6333 PDH_1636 VVGE 4 6334 PDH_1637 ALGE 46335 PDH_1638 IFGE 4 6336 PDH_1639 PLGE 4 6337 PDH_1640 ILGE 4 6338PDH_1641 PVGE 4 6339 PDH_1642 RVGE 4 6340 PDH_1643 LLGE 4 6341 PDH_1644SVGE 4 6342 PDH_1645 GLGE 4 6343 PDH_1646 IVGE 4 6344 PDH_1647 RLGE 44068 PDH_1648 LVGE 4 6345 PDH_1649 SLGE 4 6346 PDH_1650 TLGE 4 6347PDH_1651 VLGE 4 6348 PDH_1652 TVGE 4 6349 PDH_1653 GVGE 4 6350 PDH_1654AVGE 4 6351 PDH_1655 PYSY 4 6352 PDH_1656 PYSD 4 6353 PDH_1657 DYSD 46354 PDH_1658 DYSA 4 6355 PDH_1659 SYSD 4 6356 PDH_1660 FYSY 4 6357PDH_1661 VYSY 4 6358 PDH_1662 FYSS 4 6359 PDH_1663 SYSS 4 6360 PDH_1664YYSS 4 6361 PDH_1665 YYSY 4 6362 PDH_1666 AYSY 4 6363 PDH_1667 AYSD 46364 PDH_1668 HYSY 4 6365 PDH_1669 VYSD 4 6366 PDH_1670 FYSA 4 6367PDH_1671 SYSY 4 6368 PDH_1672 SYSA 4 6369 PDH_1673 FYSD 4 6370 PDH_1674YYSD 4 6371 PDH_1675 LYSY 4 6372 PDH_1676 YYSA 4 6373 PDH_1677 HYSS 46374 PDH_1678 DYSS 4 6375 PDH_1679 HYSA 4 6376 PDH_1680 DYSY 4 6377PDH_1681 HYSD 4 6378 PDH_1682 LYSD 4 6379 PDH_1683 AVPA 4 6380 PDH_1684AVRA 4 6381 PDH_1685 PVRA 4 6382 PDH_1686 PVPA 4 6383 PDH_1687 IVGA 43755 PDH_1688 EVPA 4 6384 PDH_1689 LVGA 4 6385 PDH_1690 IVRA 4 6386PDH_1691 QVGA 4 6387 PDH_1692 IVPA 4 6388 PDH_1693 EVGA 4 6389 PDH_1694LVPA 4 6390 PDH_1695 QVPA 4 6391 PDH_1696 AVGA 4 6392 PDH_1697 QVRA 46393 PDH_1698 TVRA 4 6394 PDH_1699 KVGA 4 6395 PDH_1700 VVPA 4 3837PDH_1701 VVGA 4 6396 PDH_1702 EVRA 4 6397 PDH_1703 LVRA 4 6398 PDH_1704VVRA 4 6399 PDH_1705 PVGA 4 6400 PDH_1706 TVGA 4 6401 PDH_1707 KVRA 46402 PDH_1708 KVPA 4 6403 PDH_1709 TVPA 4 6404 PDH_1710 GRGV 4 6405PDH_1711 SRGV 4 6406 PDH_1712 ILGV 4 6407 PDH_1713 AQGV 4 6408 PDH_1714TQGV 4 6409 PDH_1715 VQGV 4 4062 PDH_1716 PQGV 4 6410 PDH_1717 SQGV 46411 PDH_1718 RRGV 4 6412 PDH_1719 PLGV 4 6413 PDH_1720 PRGV 4 6414PDH_1721 IRGV 4 6415 PDH_1722 ALGV 4 6416 PDH_1723 VRGV 4 4018 PDH_1724TRGV 4 6417 PDH_1725 TLGV 4 6418 PDH_1726 GQGV 4 6419 PDH_1727 RQGV 46420 PDH_1728 ARGV 4 4812 PDH_1729 RLGV 4 6421 PDH_1730 LRGV 4 6422PDH_1731 SLGV 4 6423 PDH_1732 VLGV 4 6424 PDH_1733 IQGV 4 6425 PDH_1734LLGV 4 6426 PDH_1735 LQGV 4 6427 PDH_1736 GLGV 4 6428 PDH_1737 DSSW 46429 PDH_1738 DSSR 4 6430 PDH_1739 DSSV 4 6431 PDH_1740 DSST 4 6432PDH_1741 DSSM 4 6433 PDH_1742 DSSG 4 4185 PDH_1743 DSSA 4 6434 PDH_1744DSSS 4 6435 PDH_1745 DSSL 4 6436 PDH_1746 TGYS 4 6437 PDH_1747 SDYS 46438 PDH_1748 LAYS 4 6439 PDH_1749 VDYS 4 6440 PDH_1750 LDYS 4 6441PDH_1751 PDYS 4 6442 PDH_1752 SAYS 4 6443 PDH_1753 TDYS 4 6444 PDH_1754LAYS 4 6445 PDH_1755 GAYS 4 6446 PDH_1756 GDYS 4 6447 PDH_1757 VAYS 46448 PDH_1758 RDYS 4 6449 PDH_1759 IDYS 4 6450 PDH_1760 RAYS 4 6451PDH_1761 PAYS 4 6452 PDH_1762 ADYS 4 6453 PDH_1763 TAYS 4 6454 PDH_1764IGYS 4 6455 PDH_1765 GGYS 4 6456 PDH_1766 RGYS 4 6457 PDH_1767 AAYS 46458 PDH_1768 LYGS 4 6459 PDH_1769 TYGS 4 6460 PDH_1770 DYGS 4 6461PDH_1771 SYGS 4 6462 PDH_1772 FYGS 4 6463 PDH_1773 YYGS 4 3990 PDH_1774NYGS 4 6464 PDH_1775 HYGS 4 6465 PDH_1776 VYGS 4 6466 PDH_1777 AYGS 46467 PDH_1778 PYGS 4 6468 PDH_1779 IYGS 4 6469 PDH_1780 YYYS 4 6470PDH_1781 SYYS 4 6471 PDH_1782 YYYY 4 6472 PDH_1783 PYYF 4 6473 PDH_1784FYYS 4 6474 PDH_1785 NYYS 4 6475 PDH_1786 NYYF 4 6476 PDH_1787 FYYF 46477 PDH_1788 FYYY 4 6478 PDH_1789 AYYY 4 6479 PDH_1790 SYYY 4 6480PDH_1791 DYYY 4 6481 PDH_1792 AYYS 4 6482 PDH_1793 IYYY 4 6483 PDH_1794LYYY 4 6484 PDH_1795 DYYS 4 6485 PDH_1796 AYYF 4 6486 PDH_1797 PYYS 46487 PDH_1798 IYYS 4 6488 PDH_1799 YYYF 4 6489 PDH_1800 HYYS 4 6490PDH_1801 PYYY 4 6491 PDH_1802 HYYF 4 6492 PDH_1803 HYYY 4 6493 PDH_1804LYYS 4 6494 PDH_1805 VYYF 4 6495 PDH_1806 SYYF 4 6496 PDH_1807 IYYF 46497 PDH_1808 LYYF 4 6498 PDH_1809 DYYF 4 6499 PDH_1810 TYYS 4 6500PDH_1811 NYYY 4 6501 PDH_1812 TYYF 4 6502 PDH_1813 TYYY 4 6503 PDH_1814VYYS 4 6504 PDH_1815 VYYY 4 6505 PDH_1816 FSYS 4 6506 PDH_1817 VRYS 46507 PDH_1818 YSYS 4 6508 PDH_1819 LRYS 4 6509 PDH_1820 ARYS 4 6510PDH_1821 FRYS 4 6511 PDH_1822 SRYS 4 6512 PDH_1823 DSYS 4 6513 PDH_1824LSYS 4 6514 PDH_1825 HRYS 4 6515 PDH_1826 PSYS 4 6516 PDH_1827 HSYS 46517 PDH_1828 ASYS 4 6518 PDH_1829 YRYS 4 6519 PDH_1830 SSYS 4 6520PDH_1831 PRYS 4 6521 PDH_1832 VSYS 4 6522 PDH_1833 DRYS 4 6523 PDH_1834TWFG 4 6524 PDH_1835 GWFG 4 6525 PDH_1836 RWFG 4 6526 PDH_1837 PWFG 46527 PDH_1838 LWFG 4 3965 PDH_1839 VWFG 4 6528 PDH_1840 SWFG 4 6529PDH_1841 AWFG 4 6530 PDH_1842 IWFG 4 6531 PDH_1843 RYYY 4 6532 PDH_1844RYYS 4 6533 PDH_1845 RYYF 4 6534 PDH_1846 GYYF 4 6535 PDH_1847 GYYS 46536 PDH_1848 GYYY 4 4188 PDH_1849 KFGG 4 6537 PDH_1850 ALGG 4 6538PDH_1851 ILGG 4 6539 PDH_1852 EFGG 4 6540 PDH_1853 QFGG 4 6541 PDH_1854PLGG 4 6542 PDH_1855 VLGG 4 6543 PDH_1856 IVGG 4 6544 PDH_1857 LLGG 46545 PDH_1858 QLGG 4 6546 PDH_1859 KLGG 4 6547 PDH_1860 IFGG 4 6548PDH_1861 ELGG 4 3713 PDH_1862 PFGG 4 6549 PDH_1863 TLGG 4 6550 PDH_1864YMVRD 5 6551 PDH_1865 SMVRG 5 6552 PDH_1866 FMVRD 5 6553 PDH_1867 FMVRA5 6554 PDH_1868 DMVRA 5 6555 PDH_1869 HMVRG 5 6556 PDH_1870 LMVRG 5 6557PDH_1871 DMVRD 5 6558 PDH_1872 LMVRA 5 6559 PDH_1873 DMVRG 5 6560PDH_1874 AMVRA 5 6561 PDH_1875 AMVRD 5 6562 PDH_1876 NMVRA 5 6563PDH_1877 NMVRG 5 6564 PDH_1878 FMVRG 5 6565 PDH_1879 SMVRD 5 6566PDH_1880 YMVRA 5 6567 PDH_1881 SMVRA 5 6568 PDH_1882 PMVRD 5 6569PDH_1883 YMVRG 5 6570 PDH_1884 TMVRG 5 4010 PDH_1885 PMVRA 5 6571PDH_1886 PMVRG 5 6572 PDH_1887 VMVRA 5 6573 PDH_1888 NMVRD 5 6574PDH_1889 VMVRG 5 6575 PDH_1890 TMVRD 5 6576 PDH_1891 VMVRD 5 6577PDH_1892 TMVRA 5 6578 PDH_1893 AMVRG 5 6579 PDH_1894 LMVRD 5 6580PDH_1895 HMVRD 5 6581 PDH_1896 HMVRA 5 6582 PDH_1897 IMVRD 5 6583PDH_1898 IMVRA 5 6584 PDH_1899 IMVRG 5 6585 PDH_1900 AYGDF 5 6586PDH_1901 VYGDS 5 6587 PDH_1902 DYGDY 5 4348 PDH_1903 NYGDF 5 6588PDH_1904 DYGDS 5 6589 PDH_1905 HYGDS 5 6590 PDH_1906 HYGDY 5 6591PDH_1907 IYGDS 5 6592 PDH_1908 LYGDS 5 6593 PDH_1909 LYGDF 5 6594PDH_1910 AYGDY 5 6595 PDH_1911 AYGDS 5 6596 PDH_1912 SYGDS 5 6597PDH_1913 SYGDY 5 6598 PDH_1914 NYGDS 5 6599 PDH_1915 HYGDF 5 6600PDH_1916 FYGDS 5 6601 PDH_1917 IYGDF 5 6602 PDH_1918 FYGDY 5 6603PDH_1919 VYGDY 5 6604 PDH_1920 NYGDY 5 6605 PDH_1921 YYGDS 5 6606PDH_1922 YYGDF 5 6607 PDH_1923 PYGDY 5 6608 PDH_1924 SYGDF 5 6609PDH_1925 TYGDS 5 6610 PDH_1926 TYGDY 5 6611 PDH_1927 LYGDY 5 6612PDH_1928 DYGDF 5 6613 PDH_1929 IYGDY 5 6614 PDH_1930 VYGDF 5 6615PDH_1931 FYGDF 5 6616 PDH_1932 TYGDF 5 6617 PDH_1933 PYGDF 5 6618PDH_1934 PYGDS 5 6619 PDH_1935 YYGDY 5 6620 PDH_1936 TYSYD 5 6621PDH_1937 PYSYG 5 6622 PDH_1938 RYSYD 5 6623 PDH_1939 AYSYV 5 6624PDH_1940 PYSYA 5 6625 PDH_1941 TYSYV 5 6626 PDH_1942 PYSYD 5 6627PDH_1943 TYSYG 5 6628 PDH_1944 AYSYD 5 6629 PDH_1945 RYSYA 5 6630PDH_1946 PYSYV 5 6631 PDH_1947 GYSYG 5 4430 PDH_1948 GYSYA 5 6632PDH_1949 GYSYD 5 6633 PDH_1950 GYSYV 5 6634 PDH_1951 LYSYG 5 6635PDH_1952 SYSYV 5 6636 PDH_1953 LYSYA 5 6637 PDH_1954 LYSYD 5 6638PDH_1955 RYSYV 5 6639 PDH_1956 IYSYD 5 6640 PDH_1957 VYSYV 5 6641PDH_1958 IYSYG 5 6642 PDH_1959 IYSYA 5 6643 PDH_1960 IYSYV 5 6644PDH_1961 RYSYG 5 6645 PDH_1962 VYSYA 5 6646 PDH_1963 AYSYA 5 6647PDH_1964 SYSYA 5 6648 PDH_1965 VYSYG 5 6649 PDH_1966 AYSYG 5 6650PDH_1967 VYSYD 5 6651 PDH_1968 TYSYA 5 6652 PDH_1969 SYSYD 5 6653PDH_1970 SYSYG 5 6654 PDH_1971 LYSYV 5 6655 PDH_1972 GSGSS 5 6656PDH_1973 GSGSF 5 6657 PDH_1974 ASGSS 5 6658 PDH_1975 RSGSY 5 6659PDH_1976 NSGSY 5 6660 PDH_1977 NSGSS 5 6661 PDH_1978 TSGSS 5 6662PDH_1979 RSGSS 5 6663 PDH_1980 SSGSY 5 6664 PDH_1981 VSGSF 5 6665PDH_1982 HSGSY 5 6666 PDH_1983 TSGSY 5 6667 PDH_1984 SSGSF 5 6668PDH_1985 LSGSF 5 6669 PDH_1986 NSGSF 5 6670 PDH_1987 PSGSY 5 6671PDH_1988 TSGSF 5 6672 PDH_1989 PSGSS 5 6673 PDH_1990 PSGSF 5 6674PDH_1991 GSGSY 5 3987 PDH_1992 ISGSS 5 6675 PDH_1993 ISGSY 5 6676PDH_1994 ASGSY 5 6677 PDH_1995 RSGSF 5 6678 PDH_1996 DSGSS 5 6679PDH_1997 DSGSY 5 6680 PDH_1998 LSGSS 5 6681 PDH_1999 SSGSS 5 6682PDH_2000 HSGSF 5 6683 PDH_2001 HSGSS 5 6684 PDH_2002 LSGSY 5 6685PDH_2003 ASGSF 5 6686 PDH_2004 VSGSY 5 6687 PDH_2005 ISGSF 5 6688PDH_2006 VSGSS 5 6689 PDH_2007 DSGSF 5 6690 PDH_2008 IFLES 5 6691PDH_2009 IFLEL 5 6692 PDH_2010 RFLES 5 6693 PDH_2011 LFLEL 5 6694PDH_2012 TFLES 5 6695 PDH_2013 GFLEW 5 6696 PDH_2014 GFLES 5 6697PDH_2015 TFLEW 5 6698 PDH_2016 TFLEL 5 6699 PDH_2017 VFLEW 5 6700PDH_2018 SFLEL 5 6701 PDH_2019 VFLES 5 6702 PDH_2020 GFLEL 5 6703PDH_2021 VFLEL 5 6704 PDH_2022 AFLEL 5 6705 PDH_2023 PFLES 5 6706PDH_2024 SFLEW 5 6707 PDH_2025 AFLES 5 6708 PDH_2026 AFLEW 5 6709PDH_2027 LFLEW 5 6710 PDH_2028 PFLEL 5 6711 PDH_2029 RFLEW 5 4231PDH_2030 RFLEL 5 6712 PDH_2031 LFLES 5 6713 PDH_2032 PFLEW 5 6714PDH_2033 SFLES 5 6715 PDH_2034 IFLEW 5 6716 PDH_2035 PGSYS 5 6717PDH_2036 AGSYA 5 6718 PDH_2037 HGSYS 5 6719 PDH_2038 HGSYY 5 6720PDH_2039 PGSYY 5 6721 PDH_2040 SGSYS 5 6722 PDH_2041 SGSYD 5 6723PDH_2042 SGSYA 5 6724 PDH_2043 LGSYS 5 6725 PDH_2044 VGSYS 5 6726PDH_2045 DGSYY 5 6727 PDH_2046 FGSYS 5 6728 PDH_2047 FGSYY 5 6729PDH_2048 HGSYD 5 6730 PDH_2049 LGSYA 5 6731 PDH_2050 HGSYA 5 6732PDH_2051 LGSYD 5 6733 PDH_2052 DGSYS 5 6734 PDH_2053 VGSYD 5 6735PDH_2054 VGSYY 5 6736 PDH_2055 AGSYY 5 6737 PDH_2056 YGSYS 5 6738PDH_2057 AGSYS 5 6739 PDH_2058 AGSYD 5 6740 PDH_2059 VGSYA 5 6741PDH_2060 DGSYD 5 6742 PDH_2061 FGSYD 5 6743 PDH_2062 DGSYA 5 6744PDH_2063 YGSYA 5 6745 PDH_2064 FGSYA 5 6746 PDH_2065 YGSYD 5 6747PDH_2066 LGSYY 5 6748 PDH_2067 SGSYY 5 3761 PDH_2068 PGSYA 5 6749PDH_2069 PGSYD 5 6750 PDH_2070 YGSYY 5 6751 PDH_2071 FSGSF 5 6752PDH_2072 FSGSY 5 6753 PDH_2073 YSGSF 5 6754 PDH_2074 YSGSS 5 6755PDH_2075 FSGSS 5 6756 PDH_2076 YSGSY 5 3760 PDH_2077 ASSSW 5 6757PDH_2078 VSSSS 5 6758 PDH_2079 YSSSS 5 4478 PDH_2080 YSSSL 5 6759PDH_2081 VSSSW 5 6760 PDH_2082 ASSSL 5 6761 PDH_2083 HSSSL 5 6762PDH_2084 PSSSL 5 6763 PDH_2085 ASSSS 5 6764 PDH_2086 HSSSW 5 6765PDH_2087 VSSSL 5 6766 PDH_2088 FSSSL 5 6767 PDH_2089 HSSSS 5 6768PDH_2090 FSSSW 5 6769 PDH_2091 SSSSS 5 6770 PDH_2092 DSSSS 5 6771PDH_2093 SSSSL 5 6772 PDH_2094 FSSSS 5 6773 PDH_2095 LSSSW 5 6774PDH_2096 DSSSL 5 6775 PDH_2097 LSSSS 5 6776 PDH_2098 LSSSL 5 6777PDH_2099 SSSSW 5 6778 PDH_2100 DSSSW 5 6779 PDH_2101 PSSSW 5 6780PDH_2102 PSSSS 5 6781 PDH_2103 YSSSW 5 4439 PDH_2104 HSSGW 5 6782PDH_2105 FSSGL 5 6783 PDH_2106 SSSGW 5 6784 PDH_2107 SSSGS 5 6785PDH_2108 DSSGL 5 6786 PDH_2109 HSSGS 5 6787 PDH_2110 FSSGW 5 6788PDH_2111 ASSGW 5 6789 PDH_2112 DSSGW 5 6790 PDH_2113 DSSGS 5 6791PDH_2114 ASSGL 5 6792 PDH_2115 LSSGL 5 6793 PDH_2116 FSSGS 5 6794PDH_2117 ASSGS 5 6795 PDH_2118 PSSGS 5 6796 PDH_2119 LSSGW 5 6797PDH_2120 LSSGS 5 6798 PDH_2121 YSSGL 5 6799 PDH_2122 VSSGS 5 6800PDH_2123 PSSGW 5 6801 PDH_2124 PSSGL 5 6802 PDH_2125 VSSGL 5 6803PDH_2126 VSSGW 5 6804 PDH_2127 YSSGW 5 4460 PDH_2128 SSSGL 5 6805PDH_2129 HSSGL 5 6806 PDH_2130 YSSGS 5 6807 PDH_2131 VTVTT 5 6808PDH_2132 RTVTT 5 6809 PDH_2133 LTVTK 5 6810 PDH_2134 ATVTK 5 6811PDH_2135 GTVTT 5 6812 PDH_2136 VTVTK 5 6813 PDH_2137 LTVTR 5 6814PDH_2138 ATVTT 5 6815 PDH_2139 RTVTR 5 6816 PDH_2140 VTVTR 5 6817PDH_2141 TTVTK 5 6818 PDH_2142 GTVTR 5 6819 PDH_2143 TTVTI 5 6820PDH_2144 RTVTK 5 6821 PDH_2145 LTVTT 5 6822 PDH_2146 VTVTI 5 6823PDH_2147 GTVTK 5 6824 PDH_2148 TTVTR 5 6825 PDH_2149 GTVTI 5 6826PDH_2150 PTVTI 5 6827 PDH_2151 TTVTT 5 4351 PDH_2152 STVTT 5 6828PDH_2153 STVTI 5 6829 PDH_2154 ITVTI 5 6830 PDH_2155 STVTK 5 6831PDH_2156 STVTR 5 6832 PDH_2157 ATVTI 5 6833 PDH_2158 ITVTT 5 6834PDH_2159 ITVTR 5 6835 PDH_2160 LTVTI 5 6836 PDH_2161 PTVTR 5 6837PDH_2162 ATVTR 5 6838 PDH_2163 PTVTK 5 6839 PDH_2164 RTVTI 5 6840PDH_2165 ITVTK 5 6841 PDH_2166 PTVTT 5 6842 PDH_2167 ASSSF 5 6843PDH_2168 ASSSY 5 6844 PDH_2169 PSSSF 5 6845 PDH_2170 HSSSF 5 6846PDH_2171 VSSSY 5 6847 PDH_2172 YSSSF 5 6848 PDH_2173 FSSSF 5 6849PDH_2174 HSSSY 5 6850 PDH_2175 VSSSF 5 6851 PDH_2176 SSSSY 5 6852PDH_2177 SSSSF 5 6853 PDH_2178 LSSSY 5 6854 PDH_2179 DSSSY 5 6855PDH_2180 FSSSY 5 6856 PDH_2181 PSSSY 5 6857 PDH_2182 YSSSY 5 6858PDH_2183 DSSSF 5 6859 PDH_2184 LSSSF 5 6860 PDH_2185 FSGWF 5 6861PDH_2186 FSGWS 5 6862 PDH_2187 ISGWY 5 6863 PDH_2188 FSGWY 5 6864PDH_2189 ISGWS 5 6865 PDH_2190 PSGWS 5 6866 PDH_2191 DSGWF 5 6867PDH_2192 PSGWY 5 6868 PDH_2193 PSGWF 5 6869 PDH_2194 TSGWY 5 6870PDH_2195 ASGWF 5 6871 PDH_2196 LSGWF 5 6872 PDH_2197 ISGWF 5 6873PDH_2198 SSGWY 5 4461 PDH_2199 SSGWS 5 6874 PDH_2200 SSGWF 5 6875PDH_2201 NSGWS 5 6876 PDH_2202 NSGWY 5 6877 PDH_2203 NSGWF 5 6878PDH_2204 VSGWS 5 6879 PDH_2205 VSGWY 5 6880 PDH_2206 YSGWF 5 6881PDH_2207 LSGWS 5 6882 PDH_2208 ASGWY 5 6883 PDH_2209 LSGWY 5 6884PDH_2210 TSGWF 5 6885 PDH_2211 TSGWS 5 6886 PDH_2212 DSGWY 5 6887PDH_2213 DSGWS 5 6888 PDH_2214 ASGWS 5 6889 PDH_2215 HSGWF 5 6890PDH_2216 HSGWS 5 6891 PDH_2217 YSGWS 5 6892 PDH_2218 HSGWY 5 6893PDH_2219 YSGWY 5 6894 PDH_2220 VSGWF 5 6895 PDH_2221 ISGYD 5 6896PDH_2222 HSGYA 5 6897 PDH_2223 ISGYA 5 6898 PDH_2224 HSGYG 5 6899PDH_2225 TSGYD 5 6900 PDH_2226 TSGYA 5 6901 PDH_2227 DSGYA 5 6902PDH_2228 LSGYA 5 6903 PDH_2229 FSGYA 5 6904 PDH_2230 LSGYD 5 6905PDH_2231 FSGYG 5 6906 PDH_2232 SSGYD 5 6907 PDH_2233 SSGYA 5 6908PDH_2234 ISGYG 5 6909 PDH_2235 HSGYD 5 6910 PDH_2236 ASGYD 5 6911PDH_2237 YSGYA 5 6912 PDH_2238 YSGYD 5 4389 PDH_2239 VSGYA 5 6913PDH_2240 VSGYG 5 6914 PDH_2241 SSGYG 5 6915 PDH_2242 DSGYD 5 6916PDH_2243 DSGYG 5 6917 PDH_2244 FSGYD 5 6918 PDH_2245 LSGYG 5 6919PDH_2246 TSGYG 5 6920 PDH_2247 NSGYA 5 6921 PDH_2248 NSGYG 5 6922PDH_2249 NSGYD 5 6923 PDH_2250 PSGYA 5 6924 PDH_2251 PSGYG 5 6925PDH_2252 PSGYD 5 6926 PDH_2253 ASGYG 5 6927 PDH_2254 VSGYD 5 6928PDH_2255 ASGYA 5 6929 PDH_2256 YSGYG 5 6930 PDH_2257 FYYDS 5 6931PDH_2258 YYYDT 5 6932 PDH_2259 DYYDS 5 6933 PDH_2260 AYYDS 5 6934PDH_2261 AYYDT 5 6935 PDH_2262 DYYDI 5 6936 PDH_2263 VYYDI 5 6937PDH_2264 FYYDT 5 6938 PDH_2265 LYYDI 5 6939 PDH_2266 PYYDI 5 6940PDH_2267 HYYDI 5 6941 PDH_2268 IYYDI 5 6942 PDH_2269 LYYDS 5 6943PDH_2270 SYYDI 5 6944 PDH_2271 NYYDT 5 6945 PDH_2272 NYYDS 5 6946PDH_2273 SYYDT 5 6947 PDH_2274 AYYDI 5 6948 PDH_2275 SYYDS 5 6949PDH_2276 DYYDT 5 6950 PDH_2277 VYYDT 5 6951 PDH_2278 YYYDI 5 6952PDH_2279 VYYDS 5 6953 PDH_2280 FYYDI 5 6954 PDH_2281 YYYDS 5 4176PDH_2282 TYYDT 5 6955 PDH_2283 NYYDI 5 6956 PDH_2284 HYYDS 5 6957PDH_2285 LYYDT 5 6958 PDH_2286 IYYDT 5 6959 PDH_2287 IYYDS 5 6960PDH_2288 PYYDT 5 6961 PDH_2289 PYYDS 5 6962 PDH_2290 TYYDI 5 6963PDH_2291 HYYDT 5 6964 PDH_2292 TYYDS 5 6965 PDH_2293 DSSGY 5 4179PDH_2294 SSSGY 5 6966 PDH_2295 DSSGF 5 6967 PDH_2296 HSSGY 5 6968PDH_2297 SSSGF 5 6969 PDH_2298 ASSGF 5 6970 PDH_2299 ASSGY 5 6971PDH_2300 LSSGF 5 6972 PDH_2301 FSSGY 5 6973 PDH_2302 FSSGF 5 6974PDH_2303 YSSGF 5 6975 PDH_2304 PSSGY 5 6976 PDH_2305 VSSGY 5 6977PDH_2306 LSSGY 5 6978 PDH_2307 HSSGF 5 6979 PDH_2308 PSSGF 5 6980PDH_2309 VSSGF 5 6981 PDH_2310 YSSGY 5 6982 PDH_2311 QAARH 5 6983PDH_2312 QAARP 5 6984 PDH_2313 TAARR 5 6985 PDH_2314 QAARL 5 6986PDH_2315 KAARR 5 6987 PDH_2316 KAARP 5 6988 PDH_2317 KAARH 5 6989PDH_2318 TAARH 5 6990 PDH_2319 TAARP 5 6991 PDH_2320 EAARL 5 6992PDH_2321 EAARP 5 6993 PDH_2322 AAARL 5 6994 PDH_2323 LAARL 5 6995PDH_2324 IAARL 5 6996 PDH_2325 IAARH 5 6997 PDH_2326 LAARR 5 6998PDH_2327 LAARH 5 6999 PDH_2328 EAARH 5 7000 PDH_2329 IAARR 5 7001PDH_2330 QAARR 5 7002 PDH_2331 IAARP 5 4483 PDH_2332 EAARR 5 7003PDH_2333 KAARL 5 7004 PDH_2334 PAARR 5 7005 PDH_2335 PAARP 5 7006PDH_2336 PAARH 5 7007 PDH_2337 PAARL 5 7008 PDH_2338 VAARR 5 7009PDH_2339 AAARH 5 7010 PDH_2340 VAARP 5 7011 PDH_2341 VAARH 5 7012PDH_2342 AAARP 5 7013 PDH_2343 AAARR 5 7014 PDH_2344 TAARL 5 7015PDH_2345 LAARP 5 7016 PDH_2346 VAARL 5 7017 PDH_2347 EYYYG 5 7018PDH_2348 VYYYD 5 7019 PDH_2349 EYYYA 5 7020 PDH_2350 VYYYG 5 7021PDH_2351 EYYYD 5 7022 PDH_2352 PYYYD 5 7023 PDH_2353 PYYYG 5 7024PDH_2354 AYYYG 5 7025 PDH_2355 TYYYA 5 7026 PDH_2356 TYYYG 5 7027PDH_2357 TYYYD 5 7028 PDH_2358 QYYYA 5 7029 PDH_2359 QYYYG 5 7030PDH_2360 QYYYD 5 7031 PDH_2361 VYYYA 5 7032 PDH_2362 LYYYG 5 7033PDH_2363 LYYYD 5 7034 PDH_2364 LYYYA 5 7035 PDH_2365 AYYYD 5 7036PDH_2366 AYYYA 5 7037 PDH_2367 PYYYA 5 7038 PDH_2368 MYYYD 5 7039PDH_2369 KYYYD 5 7040 PDH_2370 KYYYG 5 7041 PDH_2371 MYYYA 5 7042PDH_2372 KYYYA 5 7043 PDH_2373 MYYYG 5 7044 PDH_2374 LTMVR 5 7045PDH_2375 RTMVQ 5 7046 PDH_2376 VTMVQ 5 7047 PDH_2377 TTMVR 5 7048PDH_2378 LTMVQ 5 7049 PDH_2379 STMVQ 5 7050 PDH_2380 ATMVQ 5 7051PDH_2381 PTMVR 5 7052 PDH_2382 RTMVR 5 7053 PDH_2383 ITMVQ 5 4055PDH_2384 GTMVR 5 7054 PDH_2385 VTMVR 5 7055 PDH_2386 PTMVQ 5 7056PDH_2387 ITMVR 5 4009 PDH_2388 ATMVR 5 7057 PDH_2389 STMVR 5 7058PDH_2390 TTMVQ 5 7059 PDH_2391 GTMVQ 5 7060 PDH_2392 HMVQG 5 7061PDH_2393 DMVQG 5 7062 PDH_2394 LMVQG 5 7063 PDH_2395 SMVQG 5 7064PDH_2396 FMVQG 5 7065 PDH_2397 NMVQG 5 7066 PDH_2398 VMVQG 5 7067PDH_2399 TMVQG 5 4056 PDH_2400 PMVQG 5 7068 PDH_2401 YMVQG 5 7069PDH_2402 AMVQG 5 7070 PDH_2403 IMVQG 5 7071 PDH_2404 PWGSY 5 7072PDH_2405 TWGSY 5 7073 PDH_2406 SWGSY 5 7074 PDH_2407 HWGSY 5 7075PDH_2408 LWGSY 5 7076 PDH_2409 DWGSY 5 7077 PDH_2410 FWGSY 5 7078PDH_2411 AWGSY 5 7079 PDH_2412 YWGSY 5 7080 PDH_2413 IWGSY 5 7081PDH_2414 NWGSY 5 7082 PDH_2415 VWGSY 5 4102 PDH_2416 FSSSWF 6 7083PDH_2417 HSSSWS 6 7084 PDH_2418 VSSSWF 6 7085 PDH_2419 HSSSWY 6 7086PDH_2420 VSSSWS 6 7087 PDH_2421 LSSSWY 6 7088 PDH_2422 VSSSWY 6 7089PDH_2423 HSSSWF 6 7090 PDH_2424 ASSSWY 6 7091 PDH_2425 DSSSWS 6 7092PDH_2426 PSSSWY 6 7093 PDH_2427 ASSSWS 6 7094 PDH_2428 DSSSWF 6 7095PDH_2429 DSSSWY 6 7096 PDH_2430 YSSSWS 6 7097 PDH_2431 YSSSWF 6 7098PDH_2432 ASSSWF 6 7099 PDH_2433 LSSSWS 6 7100 PDH_2434 SSSSWS 6 7101PDH_2435 YSSSWY 6 4437 PDH_2436 PSSSWS 6 7102 PDH_2437 SSSSWY 6 7103PDH_2438 LSSSWF 6 7104 PDH_2439 PSSSWF 6 7105 PDH_2440 FSSSWS 6 7106PDH_2441 SSSSWF 6 7107 PDH_2442 FSSSWY 6 7108 PDH_2443 AYYDST 6 7109PDH_2444 AYYDSI 6 7110 PDH_2445 YYYDST 6 7111 PDH_2446 PYYDST 6 7112PDH_2447 VYYDSS 6 7113 PDH_2448 NYYDSI 6 7114 PDH_2449 PYYDSS 6 7115PDH_2450 YYYDSS 6 4171 PDH_2451 YYYDSI 6 7116 PDH_2452 VYYDST 6 7117PDH_2453 VYYDSI 6 7118 PDH_2454 NYYDST 6 7119 PDH_2455 LYYDST 6 7120PDH_2456 NYYDSS 6 7121 PDH_2457 SYYDSI 6 7122 PDH_2458 LYYDSS 6 7123PDH_2459 SYYDST 6 7124 PDH_2460 LYYDSI 6 7125 PDH_2461 SYYDSS 6 7126PDH_2462 DYYDSI 6 7127 PDH_2463 DYYDST 6 7128 PDH_2464 DYYDSS 6 7129PDH_2465 FYYDSS 6 7130 PDH_2466 TYYDSI 6 7131 PDH_2467 FYYDST 6 7132PDH_2468 IYYDST 6 7133 PDH_2469 FYYDSI 6 7134 PDH_2470 IYYDSS 6 7135PDH_2471 TYYDSS 6 7136 PDH_2472 TYYDST 6 7137 PDH_2473 HYYDSS 6 7138PDH_2474 HYYDST 6 7139 PDH_2475 IYYDSI 6 7140 PDH_2476 HYYDSI 6 7141PDH_2477 PYYDSI 6 7142 PDH_2478 AYYDSS 6 7143 PDH_2479 LYSGYA 6 7144PDH_2480 GYSGYV 6 7145 PDH_2481 SYSGYD 6 7146 PDH_2482 LYSGYD 6 7147PDH_2483 IYSGYV 6 7148 PDH_2484 SYSGYG 6 7149 PDH_2485 IYSGYG 6 7150PDH_2486 SYSGYA 6 7151 PDH_2487 PYSGYG 6 7152 PDH_2488 VYSGYD 6 7153PDH_2489 PYSGYA 6 7154 PDH_2490 VYSGYV 6 7155 PDH_2491 GYSGYG 6 7156PDH_2492 VYSGYA 6 7157 PDH_2493 PYSGYV 6 7158 PDH_2494 GYSGYA 6 7159PDH_2495 IYSGYD 6 7160 PDH_2496 PYSGYD 6 7161 PDH_2497 RYSGYV 6 7162PDH_2498 RYSGYA 6 7163 PDH_2499 RYSGYG 6 7164 PDH_2500 GYSGYD 6 4386PDH_2501 IYSGYA 6 7165 PDH_2502 AYSGYA 6 7166 PDH_2503 RYSGYD 6 7167PDH_2504 AYSGYG 6 7168 PDH_2505 AYSGYD 6 7169 PDH_2506 AYSGYV 6 7170PDH_2507 VYSGYG 6 7171 PDH_2508 TYSGYV 6 7172 PDH_2509 TYSGYD 6 7173PDH_2510 TYSGYA 6 7174 PDH_2511 TYSGYG 6 7175 PDH_2512 SYSGYV 6 7176PDH_2513 LYSGYV 6 7177 PDH_2514 LYSGYG 6 7178 PDH_2515 SSSGWF 6 7179PDH_2516 PSSGWY 6 7180 PDH_2517 FSSGWF 6 7181 PDH_2518 PSSGWS 6 7182PDH_2519 FSSGWS 6 7183 PDH_2520 ASSGWY 6 7184 PDH_2521 FSSGWY 6 7185PDH_2522 ASSGWS 6 7186 PDH_2523 SSSGWS 6 7187 PDH_2524 YSSGWY 6 4458PDH_2525 SSSGWY 6 7188 PDH_2526 HSSGWS 6 7189 PDH_2527 DSSGWS 6 7190PDH_2528 YSSGWS 6 7191 PDH_2529 LSSGWF 6 7192 PDH_2530 HSSGWY 6 7193PDH_2531 DSSGWF 6 7194 PDH_2532 DSSGWY 6 7195 PDH_2533 LSSGWY 6 7196PDH_2534 HSSGWF 6 7197 PDH_2535 VSSGWS 6 7198 PDH_2536 LSSGWS 6 7199PDH_2537 VSSGWY 6 7200 PDH_2538 VSSGWF 6 7201 PDH_2539 PSSGWF 6 7202PDH_2540 ASSGWF 6 7203 PDH_2541 YSSGWF 6 7204 PDH_2542 IYYGSA 6 7205PDH_2543 PYYGSG 6 7206 PDH_2544 PYYGSA 6 7207 PDH_2545 PYYGSD 6 7208PDH_2546 VYYGSD 6 7209 PDH_2547 AYYGSA 6 7210 PDH_2548 VYYGSA 6 7211PDH_2549 NYYGSA 6 7212 PDH_2550 YYYGSA 6 7213 PDH_2551 AYYGSD 6 7214PDH_2552 NYYGSD 6 7215 PDH_2553 YYYGSD 6 7216 PDH_2554 FYYGSD 6 7217PDH_2555 NYYGSG 6 7218 PDH_2556 YYYGSG 6 3979 PDH_2557 FYYGSA 6 7219PDH_2558 FYYGSG 6 7220 PDH_2559 AYYGSG 6 7221 PDH_2560 LYYGSG 6 7222PDH_2561 DYYGSD 6 7223 PDH_2562 LYYGSA 6 7224 PDH_2563 TYYGSD 6 7225PDH_2564 DYYGSG 6 7226 PDH_2565 DYYGSA 6 7227 PDH_2566 LYYGSD 6 7228PDH_2567 HYYGSD 6 7229 PDH_2568 TYYGSA 6 7230 PDH_2569 TYYGSG 6 7231PDH_2570 HYYGSA 6 7232 PDH_2571 SYYGSG 6 7233 PDH_2572 HYYGSG 6 7234PDH_2573 SYYGSD 6 7235 PDH_2574 VYYGSG 6 7236 PDH_2575 SYYGSA 6 7237PDH_2576 IYYGSG 6 7238 PDH_2577 IYYGSD 6 7239 PDH_2578 VSSGYS 6 7240PDH_2579 VSSGYY 6 7241 PDH_2580 FSSGYY 6 7242 PDH_2581 ASSGYF 6 7243PDH_2582 ASSGYS 6 7244 PDH_2583 FSSGYS 6 7245 PDH_2584 SSSGYY 6 7246PDH_2585 ASSGYY 6 7247 PDH_2586 FSSGYF 6 7248 PDH_2587 HSSGYY 6 7249PDH_2588 DSSGYY 6 4174 PDH_2589 HSSGYS 6 7250 PDH_2590 SSSGYS 6 7251PDH_2591 DSSGYS 6 7252 PDH_2592 DSSGYF 6 7253 PDH_2593 HSSGYF 6 7254PDH_2594 PSSGYF 6 7255 PDH_2595 SSSGYF 6 7256 PDH_2596 YSSGYY 6 7257PDH_2597 YSSGYF 6 7258 PDH_2598 YSSGYS 6 7259 PDH_2599 PSSGYS 6 7260PDH_2600 PSSGYY 6 7261 PDH_2601 LSSGYY 6 7262 PDH_2602 LSSGYF 6 7263PDH_2603 LSSGYS 6 7264 PDH_2604 VSSGYF 6 7265 PDH_2605 TYDSSD 6 7266PDH_2606 YYDSSA 6 7267 PDH_2607 YYDSSG 6 4172 PDH_2608 LYDSSD 6 7268PDH_2609 LYDSSA 6 7269 PDH_2610 PYDSSA 6 7270 PDH_2611 LYDSSG 6 7271PDH_2612 PYDSSD 6 7272 PDH_2613 VYDSSD 6 7273 PDH_2614 HYDSSG 6 7274PDH_2615 VYDSSG 6 7275 PDH_2616 VYDSSA 6 7276 PDH_2617 PYDSSG 6 7277PDH_2618 SYDSSA 6 7278 PDH_2619 FYDSSG 6 7279 PDH_2620 NYDSSA 6 7280PDH_2621 SYDSSG 6 7281 PDH_2622 FYDSSA 6 7282 PDH_2623 NYDSSD 6 7283PDH_2624 SYDSSD 6 7284 PDH_2625 FYDSSD 6 7285 PDH_2626 DYDSSG 6 7286PDH_2627 AYDSSG 6 7287 PDH_2628 NYDSSG 6 7288 PDH_2629 IYDSSA 6 7289PDH_2630 DYDSSD 6 7290 PDH_2631 IYDSSG 6 7291 PDH_2632 AYDSSD 6 7292PDH_2633 DYDSSA 6 7293 PDH_2634 IYDSSD 6 7294 PDH_2635 HYDSSA 6 7295PDH_2636 AYDSSA 6 7296 PDH_2637 HYDSSD 6 7297 PDH_2638 TYDSSG 6 7298PDH_2639 TYDSSA 6 7299 PDH_2640 YYDSSD 6 7300 PDH_2641 TDFWSA 6 7301PDH_2642 ADFWSG 6 7302 PDH_2643 PDFWSG 6 7303 PDH_2644 VDFWSD 6 7304PDH_2645 TDFWSD 6 7305 PDH_2646 HDFWSA 6 7306 PDH_2647 HDFWSD 6 7307PDH_2648 TDFWSG 6 7308 PDH_2649 LDFWSA 6 7309 PDH_2650 LDFWSG 6 7310PDH_2651 HDFWSG 6 7311 PDH_2652 LDFWSD 6 7312 PDH_2653 FDFWSG 6 7313PDH_2654 DDFWSD 6 7314 PDH_2655 VDFWSA 6 7315 PDH_2656 SDFWSD 6 7316PDH_2657 SDFWSG 6 7317 PDH_2658 NDFWSD 6 7318 PDH_2659 SDFWSA 6 7319PDH_2660 NDFWSA 6 7320 PDH_2661 NDFWSG 6 7321 PDH_2662 VDFWSG 6 7322PDH_2663 IDFWSD 6 7323 PDH_2664 IDFWSG 6 7324 PDH_2665 FDFWSA 6 7325PDH_2666 ADFWSD 6 7326 PDH_2667 IDFWSA 6 7327 PDH_2668 FDFWSD 6 7328PDH_2669 DDFWSG 6 7329 PDH_2670 ADFWSA 6 7330 PDH_2671 YDFWSA 6 7331PDH_2672 PDFWSD 6 7332 PDH_2673 YDFWSG 6 4253 PDH_2674 DDFWSA 6 7333PDH_2675 PDFWSA 6 7334 PDH_2676 YDFWSD 6 7335 PDH_2677 DDSSGY 6 7336PDH_2678 LDSSGY 6 7337 PDH_2679 HDSSGS 6 7338 PDH_2680 DDSSGF 6 7339PDH_2681 DDSSGS 6 7340 PDH_2682 LDSSGS 6 7341 PDH_2683 HDSSGY 6 7342PDH_2684 SDSSGS 6 7343 PDH_2685 SDSSGF 6 7344 PDH_2686 PDSSGS 6 7345PDH_2687 SDSSGY 6 7346 PDH_2688 PDSSGY 6 7347 PDH_2689 ADSSGY 6 7348PDH_2690 ADSSGS 6 7349 PDH_2691 ADSSGF 6 7350 PDH_2692 YDSSGF 6 7351PDH_2693 VDSSGF 6 7352 PDH_2694 FDSSGF 6 7353 PDH_2695 VDSSGY 6 7354PDH_2696 YDSSGY 6 4173 PDH_2697 FDSSGS 6 7355 PDH_2698 VDSSGS 6 7356PDH_2699 YDSSGS 6 7357 PDH_2700 FDSSGY 6 7358 PDH_2701 LDSSGF 6 7359PDH_2702 HDSSGF 6 7360 PDH_2703 PDSSGF 6 7361 PDH_2704 EYFDWS 6 7362PDH_2705 QYFDWL 6 7363 PDH_2706 RYFDWF 6 7364 PDH_2707 AYFDWF 6 7365PDH_2708 PYFDWF 6 7366 PDH_2709 PYFDWP 6 7367 PDH_2710 LYFDWP 6 7368PDH_2711 EYFDWP 6 7369 PDH_2712 EYFDWL 6 7370 PDH_2713 RYFDWL 6 4305PDH_2714 LYFDWL 6 7371 PDH_2715 PYFDWL 6 7372 PDH_2716 LYFDWS 6 7373PDH_2717 QYFDWS 6 7374 PDH_2718 QYFDWF 6 7375 PDH_2719 VYFDWF 6 7376PDH_2720 RYFDWP 6 7377 PDH_2721 AYFDWP 6 7378 PDH_2722 LYFDWF 6 7379PDH_2723 AYFDWL 6 7380 PDH_2724 GYFDWF 6 7381 PDH_2725 GYFDWS 6 7382PDH_2726 VYFDWL 6 7383 PDH_2727 VYFDWS 6 7384 PDH_2728 VYFDWP 6 7385PDH_2729 RYFDWS 6 7386 PDH_2730 PYFDWS 6 7387 PDH_2731 QYFDWP 6 7388PDH_2732 EYFDWF 6 7389 PDH_2733 AYFDWS 6 7390 PDH_2734 GYFDWL 6 7391PDH_2735 GYFDWP 6 7392 PDH_2736 CGSTSC 6 7393 PDH_2737 CSGTSC 6 7394PDH_2738 CSSTSC 6 3814 PDH_2739 CGGTSC 6 7395 PDH_2740 PYYGSE 6 7396PDH_2741 VYYGSE 6 7397 PDH_2742 AYYGSE 6 7398 PDH_2743 NYYGSE 6 7399PDH_2744 FYYGSE 6 7400 PDH_2745 YYYGSE 6 7401 PDH_2746 DYYGSE 6 7402PDH_2747 TYYGSE 6 7403 PDH_2748 HYYGSE 6 7404 PDH_2749 LYYGSE 6 7405PDH_2750 IYYGSE 6 7406 PDH_2751 SYYGSE 6 7407 PDH_2752 CSSGSC 6 7408PDH_2753 CGGGSC 6 7409 PDH_2754 CGSGSC 6 7410 PDH_2755 CSGGSC 6 3775PDH_2756 PFWSGS 6 7411 PDH_2757 DFWSGF 6 7412 PDH_2758 PFWSGY 6 7413PDH_2759 AFWSGY 6 7414 PDH_2760 AFWSGS 6 7415 PDH_2761 AFWSGF 6 7416PDH_2762 PFWSGF 6 7417 PDH_2763 VFWSGY 6 7418 PDH_2764 YFWSGF 6 7419PDH_2765 IFWSGS 6 7420 PDH_2766 VFWSGF 6 7421 PDH_2767 VFWSGS 6 7422PDH_2768 IFWSGF 6 7423 PDH_2769 IFWSGY 6 7424 PDH_2770 SFWSGF 6 7425PDH_2771 YFWSGY 6 7426 PDH_2772 FFWSGS 6 7427 PDH_2773 YFWSGS 6 7428PDH_2774 FFWSGY 6 7429 PDH_2775 LFWSGF 6 7430 PDH_2776 TFWSGF 6 7431PDH_2777 NFWSGF 6 7432 PDH_2778 HFWSGF 6 7433 PDH_2779 NFWSGS 6 7434PDH_2780 SFWSGS 6 7435 PDH_2781 TFWSGS 6 7436 PDH_2782 NFWSGY 6 7437PDH_2783 DFWSGY 6 4254 PDH_2784 LFWSGY 6 7438 PDH_2785 HFWSGS 6 7439PDH_2786 SFWSGY 6 7440 PDH_2787 TFWSGY 6 7441 PDH_2788 DFWSGS 6 7442PDH_2789 LFWSGS 6 7443 PDH_2790 HFWSGY 6 7444 PDH_2791 FFWSGF 6 7445PDH_2792 DYYDSSG 7 7446 PDH_2793 SYYDSSG 7 7447 PDH_2794 IYYDSSG 7 7448PDH_2795 HYYDSSG 7 7449 PDH_2796 SYYDSSA 7 7450 PDH_2797 SYYDSSD 7 7451PDH_2798 IYYDSSA 7 7452 PDH_2799 AYYDSSA 7 7453 PDH_2800 IYYDSSD 7 7454PDH_2801 AYYDSSD 7 7455 PDH_2802 HYYDSSD 7 7456 PDH_2803 VYYDSSD 7 7457PDH_2804 HYYDSSA 7 7458 PDH_2805 AYYDSSG 7 7459 PDH_2806 VYYDSSA 7 7460PDH_2807 LYYDSSG 7 7461 PDH_2808 VYYDSSG 7 7462 PDH_2809 LYYDSSD 7 7463PDH_2810 FYYDSSG 7 7464 PDH_2811 LYYDSSA 7 7465 PDH_2812 FYYDSSD 7 7466PDH_2813 TYYDSSG 7 7467 PDH_2814 PYYDSSG 7 7468 PDH_2815 FYYDSSA 7 7469PDH_2816 TYYDSSD 7 7470 PDH_2817 NYYDSSG 7 7471 PDH_2818 TYYDSSA 7 7472PDH_2819 PYYDSSA 7 7473 PDH_2820 YYYDSSG 7 4167 PDH_2821 PYYDSSD 7 7474PDH_2822 NYYDSSA 7 7475 PDH_2823 YYYDSSD 7 7476 PDH_2824 NYYDSSD 7 7477PDH_2825 YYYDSSA 7 7478 PDH_2826 DYYDSSD 7 7479 PDH_2827 DYYDSSA 7 7480PDH_2828 FDILTGF 7 7481 PDH_2829 FDILTGS 7 7482 PDH_2830 LDILTGY 7 7483PDH_2831 YDILTGF 7 7484 PDH_2832 LDILTGS 7 7485 PDH_2833 SDILTGF 7 7486PDH_2834 IDILTGS 7 7487 PDH_2835 PDILTGS 7 7488 PDH_2836 PDILTGF 7 7489PDH_2837 IDILTGF 7 7490 PDH_2838 IDILTGY 7 7491 PDH_2839 PDILTGY 7 7492PDH_2840 YDILTGY 7 4325 PDH_2841 DDILTGF 7 7493 PDH_2842 YDILTGS 7 7494PDH_2843 HDILTGY 7 7495 PDH_2844 TDILTGF 7 7496 PDH_2845 VDILTGY 7 7497PDH_2846 LDILTGF 7 7498 PDH_2847 VDILTGS 7 7499 PDH_2848 HDILTGS 7 7500PDH_2849 ADILTGS 7 7501 PDH_2850 HDILTGF 7 7502 PDH_2851 NDILTGS 7 7503PDH_2852 NDILTGF 7 7504 PDH_2853 ADILTGY 7 7505 PDH_2854 VDILTGF 7 7506PDH_2855 TDILTGY 7 7507 PDH_2856 ADILTGF 7 7508 PDH_2857 NDILTGY 7 7509PDH_2858 TDILTGS 7 7510 PDH_2859 SDILTGS 7 7511 PDH_2860 DDILTGY 7 7512PDH_2861 SDILTGY 7 7513 PDH_2862 DDILTGS 7 7514 PDH_2863 FDILTGY 7 7515PDH_2864 SYDFWSA 7 7516 PDH_2865 FYDFWSG 7 7517 PDH_2866 IYDFWSD 7 7518PDH_2867 LYDFWSD 7 7519 PDH_2868 AYDFWSD 7 7520 PDH_2869 IYDFWSA 7 7521PDH_2870 PYDFWSD 7 7522 PDH_2871 AYDFWSA 7 7523 PDH_2872 SYDFWSG 7 7524PDH_2873 FYDFWSA 7 7525 PDH_2874 PYDFWSA 7 7526 PDH_2875 YYDFWSG 7 4248PDH_2876 FYDFWSD 7 7527 PDH_2877 IYDFWSG 7 7528 PDH_2878 PYDFWSG 7 7529PDH_2879 YYDFWSA 7 7530 PDH_2880 YYDFWSD 7 7531 PDH_2881 NYDFWSD 7 7532PDH_2882 VYDFWSA 7 7533 PDH_2883 NYDFWSA 7 7534 PDH_2884 VYDFWSD 7 7535PDH_2885 VYDFWSG 7 7536 PDH_2886 NYDFWSG 7 7537 PDH_2887 DYDFWSA 7 7538PDH_2888 DYDFWSD 7 7539 PDH_2889 DYDFWSG 7 7540 PDH_2890 HYDFWSG 7 7541PDH_2891 TYDFWSG 7 7542 PDH_2892 HYDFWSA 7 7543 PDH_2893 LYDFWSG 7 7544PDH_2894 HYDFWSD 7 7545 PDH_2895 TYDFWSA 7 7546 PDH_2896 SYDFWSD 7 7547PDH_2897 TYDFWSD 7 7548 PDH_2898 LYDFWSA 7 7549 PDH_2899 AYDFWSG 7 7550PDH_2900 SCSSTSC 7 7551 PDH_2901 HCSSTSC 7 7552 PDH_2902 PCSSTSC 7 7553PDH_2903 ACSSTSC 7 7554 PDH_2904 VCSSTSC 7 7555 PDH_2905 LCSSTSC 7 7556PDH_2906 YCSSTSC 7 3812 PDH_2907 FCSSTSC 7 7557 PDH_2908 DCSSTSC 7 7558PDH_2909 RYSSSWS 7 7559 PDH_2910 VYSSSWY 7 7560 PDH_2911 GYSSSWY 7 4435PDH_2912 VYSSSWS 7 7561 PDH_2913 TYSSSWY 7 7562 PDH_2914 TYSSSWF 7 7563PDH_2915 TYSSSWS 7 7564 PDH_2916 GYSSSWF 7 7565 PDH_2917 LYSSSWF 7 7566PDH_2918 AYSSSWS 7 7567 PDH_2919 SYSSSWF 7 7568 PDH_2920 WYSSSWS 7 7569PDH_2921 WYSSSWF 7 7570 PDH_2922 MYSSSWS 7 7571 PDH_2923 SYSSSWS 7 7572PDH_2924 LYSSSWY 7 7573 PDH_2925 AYSSSWY 7 7574 PDH_2926 AYSSSWF 7 7575PDH_2927 LYSSSWS 7 7576 PDH_2928 WYSSSWY 7 7577 PDH_2929 VYSSSWF 7 7578PDH_2930 SYSSSWY 7 7579 PDH_2931 RYSSSWF 7 7580 PDH_2932 MYSSSWF 7 7581PDH_2933 RYSSSWY 7 7582 PDH_2934 MYSSSWY 7 7583 PDH_2935 GYSSSWS 7 7584PDH_2936 IYDSSGY 7 7585 PDH_2937 PYDSSGS 7 7586 PDH_2938 VYDSSGF 7 7587PDH_2939 PYDSSGY 7 7588 PDH_2940 NYDSSGS 7 7589 PDH_2941 LYDSSGY 7 7590PDH_2942 NYDSSGY 7 7591 PDH_2943 LYDSSGS 7 7592 PDH_2944 VYDSSGY 7 7593PDH_2945 VYDSSGS 7 7594 PDH_2946 LYDSSGF 7 7595 PDH_2947 NYDSSGF 7 7596PDH_2948 YYDSSGY 7 4168 PDH_2949 YYDSSGS 7 7597 PDH_2950 DYDSSGS 7 7598PDH_2951 TYDSSGY 7 7599 PDH_2952 TYDSSGF 7 7600 PDH_2953 TYDSSGS 7 7601PDH_2954 YYDSSGF 7 7602 PDH_2955 AYDSSGS 7 7603 PDH_2956 HYDSSGY 7 7604PDH_2957 AYDSSGY 7 7605 PDH_2958 HYDSSGF 7 7606 PDH_2959 HYDSSGS 7 7607PDH_2960 FYDSSGF 7 7608 PDH_2961 IYDSSGF 7 7609 PDH_2962 DYDSSGY 7 7610PDH_2963 DYDSSGF 7 7611 PDH_2964 PYDSSGF 7 7612 PDH_2965 SYDSSGS 7 7613PDH_2966 SYDSSGF 7 7614 PDH_2967 AYDSSGF 7 7615 PDH_2968 FYDSSGY 7 7616PDH_2969 SYDSSGY 7 7617 PDH_2970 IYDSSGS 7 7618 PDH_2971 FYDSSGS 7 7619PDH_2972 HYYGSGI 7 7620 PDH_2973 SYYGSGI 7 7621 PDH_2974 AYYGSGT 7 7622PDH_2975 DYYGSGI 7 7623 PDH_2976 VYYGSGT 7 7624 PDH_2977 AYYGSGS 7 7625PDH_2978 VYYGSGI 7 7626 PDH_2979 VYYGSGS 7 7627 PDH_2980 HYYGSGS 7 7628PDH_2981 LYYGSGS 7 7629 PDH_2982 HYYGSGT 7 7630 PDH_2983 LYYGSGT 7 7631PDH_2984 PYYGSGS 7 7632 PDH_2985 SYYGSGS 7 7633 PDH_2986 NYYGSGS 7 7634PDH_2987 IYYGSGS 7 7635 PDH_2988 DYYGSGT 7 7636 PDH_2989 PYYGSGI 7 7637PDH_2990 PYYGSGT 7 7638 PDH_2991 SYYGSGT 7 7639 PDH_2992 FYYGSGS 7 7640PDH_2993 YYYGSGS 7 3975 PDH_2994 NYYGSGT 7 7641 PDH_2995 IYYGSGT 7 7642PDH_2996 DYYGSGS 7 7643 PDH_2997 TYYGSGS 7 7644 PDH_2998 FYYGSGT 7 7645PDH_2999 YYYGSGT 7 7646 PDH_3000 TYYGSGT 7 7647 PDH_3001 YYYGSGI 7 7648PDH_3002 FYYGSGI 7 7649 PDH_3003 AYYGSGI 7 7650 PDH_3004 TYYGSGI 7 7651PDH_3005 IYYGSGI 7 7652 PDH_3006 LYYGSGI 7 7653 PDH_3007 NYYGSGI 7 7654PDH_3008 TYSSGWF 7 7655 PDH_3009 TYSSGWS 7 7656 PDH_3010 VYSSGWF 7 7657PDH_3011 GYSSGWF 7 7658 PDH_3012 MYSSGWS 7 7659 PDH_3013 SYSSGWF 7 7660PDH_3014 MYSSGWY 7 7661 PDH_3015 LYSSGWY 7 7662 PDH_3016 MYSSGWF 7 7663PDH_3017 WYSSGWS 7 7664 PDH_3018 WYSSGWY 7 7665 PDH_3019 AYSSGWS 7 7666PDH_3020 RYSSGWF 7 7667 PDH_3021 LYSSGWF 7 7668 PDH_3022 AYSSGWY 7 7669PDH_3023 RYSSGWY 7 7670 PDH_3024 SYSSGWY 7 7671 PDH_3025 RYSSGWS 7 7672PDH_3026 GYSSGWY 7 4456 PDH_3027 WYSSGWF 7 7673 PDH_3028 AYSSGWF 7 7674PDH_3029 GYSSGWS 7 7675 PDH_3030 VYSSGWY 7 7676 PDH_3031 VYSSGWS 7 7677PDH_3032 LYSSGWS 7 7678 PDH_3033 TYSSGWY 7 7679 PDH_3034 SYSSGWS 7 7680PDH_3035 FCSGGSC 7 7681 PDH_3036 LCSGGSC 7 7682 PDH_3037 SCSGGSC 7 7683PDH_3038 YCSGGSC 7 3773 PDH_3039 DCSGGSC 7 7684 PDH_3040 HCSGGSC 7 7685PDH_3041 PCSGGSC 7 7686 PDH_3042 ACSGGSC 7 7687 PDH_3043 VCSGGSC 7 7688PDH_3044 CSGGSCH 7 7689 PDH_3045 CSGGSCL 7 7690 PDH_3046 CSGGSCS 7 7691PDH_3047 CSGGSCY 7 3774 PDH_3048 CSGGSCP 7 7692 PDH_3049 CSGGSCF 7 7693PDH_3050 CSSTSCY 7 3813 PDH_3051 CSSTSCL 7 7694 PDH_3052 CSSTSCF 7 7695PDH_3053 CSSTSCH 7 7696 PDH_3054 CSSTSCS 7 7697 PDH_3055 CSSTSCP 7 7698PDH_3056 ICGGDCF 7 7699 PDH_3057 VCGGDCS 7 7700 PDH_3058 DCGGDCF 7 7701PDH_3059 SCGGDCF 7 7702 PDH_3060 NCGGDCY 7 7703 PDH_3061 VCGGDCY 7 7704PDH_3062 SCGGDCS 7 7705 PDH_3063 NCGGDCS 7 7706 PDH_3064 HCGGDCF 7 7707PDH_3065 LCGGDCS 7 7708 PDH_3066 LCGGDCY 7 7709 PDH_3067 ICGGDCY 7 7710PDH_3068 LCGGDCF 7 7711 PDH_3069 ICGGDCS 7 7712 PDH_3070 VCGGDCF 7 7713PDH_3071 HCGGDCS 7 7714 PDH_3072 YCGGDCF 7 7715 PDH_3073 HCGGDCY 7 7716PDH_3074 FCGGDCF 7 7717 PDH_3075 YCGGDCS 7 7718 PDH_3076 DCGGDCS 7 7719PDH_3077 YCGGDCY 7 3866 PDH_3078 DCGGDCY 7 7720 PDH_3079 PCGGDCF 7 7721PDH_3080 FCGGDCS 7 7722 PDH_3081 FCGGDCY 7 7723 PDH_3082 ACGGDCF 7 7724PDH_3083 ACGGDCY 7 7725 PDH_3084 ACGGDCS 7 7726 PDH_3085 PCGGDCY 7 7727PDH_3086 PCGGDCS 7 7728 PDH_3087 SCGGDCY 7 7729 PDH_3088 NCGGDCF 7 7730PDH_3089 TCGGDCS 7 7731 PDH_3090 TCGGDCY 7 7732 PDH_3091 TCGGDCF 7 7733PDH_3092 NDFWSGF 7 7734 PDH_3093 HDFWSGF 7 7735 PDH_3094 SDFWSGF 7 7736PDH_3095 NDFWSGY 7 7737 PDH_3096 HDFWSGS 7 7738 PDH_3097 DDFWSGF 7 7739PDH_3098 HDFWSGY 7 7740 PDH_3099 SDFWSGY 7 7741 PDH_3100 FDFWSGF 7 7742PDH_3101 PDFWSGY 7 7743 PDH_3102 IDFWSGF 7 7744 PDH_3103 SDFWSGS 7 7745PDH_3104 PDFWSGS 7 7746 PDH_3105 TDFWSGF 7 7747 PDH_3106 LDFWSGS 7 7748PDH_3107 DDFWSGY 7 7749 PDH_3108 LDFWSGY 7 7750 PDH_3109 FDFWSGS 7 7751PDH_3110 ADFWSGF 7 7752 PDH_3111 FDFWSGY 7 7753 PDH_3112 TDFWSGS 7 7754PDH_3113 PDFWSGF 7 7755 PDH_3114 VDFWSGF 7 7756 PDH_3115 TDFWSGY 7 7757PDH_3116 IDFWSGY 7 7758 PDH_3117 DDFWSGS 7 7759 PDH_3118 YDFWSGS 7 7760PDH_3119 YDFWSGF 7 7761 PDH_3120 IDFWSGS 7 7762 PDH_3121 NDFWSGS 7 7763PDH_3122 YDFWSGY 7 4249 PDH_3123 LDFWSGF 7 7764 PDH_3124 ADFWSGY 7 7765PDH_3125 VDFWSGS 7 7766 PDH_3126 ADFWSGS 7 7767 PDH_3127 VDFWSGY 7 7768PDH_3128 YDSSGYS 7 7769 PDH_3129 VDSSGYF 7 7770 PDH_3130 VDSSGYS 7 7771PDH_3131 ADSSGYS 7 7772 PDH_3132 HDSSGYF 7 7773 PDH_3133 ADSSGYY 7 7774PDH_3134 DDSSGYY 7 7775 PDH_3135 DDSSGYS 7 7776 PDH_3136 SDSSGYS 7 7777PDH_3137 SDSSGYF 7 7778 PDH_3138 PDSSGYS 7 7779 PDH_3139 SDSSGYY 7 7780PDH_3140 PDSSGYY 7 7781 PDH_3141 HDSSGYY 7 7782 PDH_3142 PDSSGYF 7 7783PDH_3143 FDSSGYF 7 7784 PDH_3144 HDSSGYS 7 7785 PDH_3145 ADSSGYF 7 7786PDH_3146 DDSSGYF 7 7787 PDH_3147 LDSSGYF 7 7788 PDH_3148 LDSSGYY 7 7789PDH_3149 LDSSGYS 7 7790 PDH_3150 FDSSGYY 7 7791 PDH_3151 FDSSGYS 7 7792PDH_3152 YDSSGYF 7 7793 PDH_3153 YDSSGYY 7 4169 PDH_3154 VDSSGYY 7 7794PDH_3155 EYCGGDC 7 7795 PDH_3156 QYCGGDC 7 7796 PDH_3157 AYCGGDC 7 3865PDH_3158 IYCGGDC 7 7797 PDH_3159 TYCGGDC 7 7798 PDH_3160 PYCGGDC 7 7799PDH_3161 LYCGGDC 7 7800 PDH_3162 VYCGGDC 7 7801 PDH_3163 KYCGGDC 7 7802PDH_3164 VYYDSSGF 8 7803 PDH_3165 IYYDSSGF 8 7804 PDH_3166 YYYDSSGY 84164 PDH_3167 PYYDSSGF 8 7805 PDH_3168 HYYDSSGF 8 7806 PDH_3169 HYYDSSGY8 7807 PDH_3170 AYYDSSGF 8 7808 PDH_3171 HYYDSSGS 8 7809 PDH_3172DYYDSSGY 8 7810 PDH_3173 DYYDSSGS 8 7811 PDH_3174 YYYDSSGS 8 7812PDH_3175 SYYDSSGF 8 7813 PDH_3176 LYYDSSGF 8 7814 PDH_3177 NYYDSSGF 87815 PDH_3178 NYYDSSGY 8 7816 PDH_3179 IYYDSSGY 8 7817 PDH_3180 SYYDSSGS8 7818 PDH_3181 AYYDSSGS 8 7819 PDH_3182 IYYDSSGS 8 7820 PDH_3183AYYDSSGY 8 7821 PDH_3184 DYYDSSGF 8 7822 PDH_3185 SYYDSSGY 8 7823PDH_3186 NYYDSSGS 8 7824 PDH_3187 LYYDSSGY 8 7825 PDH_3188 PYYDSSGS 87826 PDH_3189 LYYDSSGS 8 7827 PDH_3190 PYYDSSGY 8 7828 PDH_3191 TYYDSSGY8 7829 PDH_3192 TYYDSSGS 8 7830 PDH_3193 FYYDSSGF 8 7831 PDH_3194FYYDSSGY 8 7832 PDH_3195 FYYDSSGS 8 7833 PDH_3196 VYYDSSGS 8 7834PDH_3197 TYYDSSGF 8 7835 PDH_3198 YYYDSSGF 8 7836 PDH_3199 VYYDSSGY 87837 PDH_3200 TYYGSGSS 8 7838 PDH_3201 SYYGSGSY 8 7839 PDH_3202 SYYGSGSS8 7840 PDH_3203 HYYGSGSF 8 7841 PDH_3204 PYYGSGSF 8 7842 PDH_3205AYYGSGSS 8 7843 PDH_3206 AYYGSGSF 8 7844 PDH_3207 AYYGSGSY 8 7845PDH_3208 YYYGSGSF 8 7846 PDH_3209 TYYGSGSY 8 7847 PDH_3210 YYYGSGSY 83972 PDH_3211 PYYGSGSS 8 7848 PDH_3212 PYYGSGSY 8 7849 PDH_3213 DYYGSGSY8 7850 PDH_3214 VYYGSGSY 8 7851 PDH_3215 VYYGSGSF 8 7852 PDH_3216YYYGSGSS 8 7853 PDH_3217 LYYGSGSF 8 7854 PDH_3218 LYYGSGSY 8 7855PDH_3219 DYYGSGSS 8 7856 PDH_3220 VYYGSGSS 8 7857 PDH_3221 IYYGSGSS 87858 PDH_3222 FYYGSGSY 8 7859 PDH_3223 FYYGSGSS 8 7860 PDH_3224 LYYGSGSS8 7861 PDH_3225 SYYGSGSF 8 7862 PDH_3226 IYYGSGSY 8 7863 PDH_3227IYYGSGSF 8 7864 PDH_3228 FYYGSGSF 8 7865 PDH_3229 DYYGSGSF 8 7866PDH_3230 NYYGSGSS 8 7867 PDH_3231 NYYGSGSY 8 7868 PDH_3232 TYYGSGSF 87869 PDH_3233 HYYGSGSY 8 7870 PDH_3234 HYYGSGSS 8 7871 PDH_3235 NYYGSGSF8 7872 PDH_3236 AYCSSTSC 8 7873 PDH_3237 PYCSSTSC 8 7874 PDH_3238RYCSSTSC 8 7875 PDH_3239 IYCSSTSC 8 7876 PDH_3240 LYCSSTSC 8 7877PDH_3241 VYCSSTSC 8 7878 PDH_3242 TYCSSTSC 8 7879 PDH_3243 SYCSSTSC 87880 PDH_3244 GYCSSTSC 8 3809 PDH_3245 IYCSGGSC 8 7881 PDH_3246 VYCSGGSC8 7882 PDH_3247 LYCSGGSC 8 7883 PDH_3248 AYCSGGSC 8 7884 PDH_3249TYCSGGSC 8 7885 PDH_3250 GYCSGGSC 8 3770 PDH_3251 RYCSGGSC 8 7886PDH_3252 PYCSGGSC 8 7887 PDH_3253 SYCSGGSC 8 7888 PDH_3254 FYDFWSGY 87889 PDH_3255 AYDFWSGF 8 7890 PDH_3256 NYDFWSGF 8 7891 PDH_3257 IYDFWSGF8 7892 PDH_3258 DYDFWSGS 8 7893 PDH_3259 DYDFWSGY 8 7894 PDH_3260NYDFWSGY 8 7895 PDH_3261 VYDFWSGF 8 7896 PDH_3262 VYDFWSGS 8 7897PDH_3263 YYDFWSGF 8 7898 PDH_3264 PYDFWSGF 8 7899 PDH_3265 FYDFWSGS 87900 PDH_3266 HYDFWSGF 8 7901 PDH_3267 HYDFWSGY 8 7902 PDH_3268 YYDFWSGS8 7903 PDH_3269 PYDFWSGY 8 7904 PDH_3270 PYDFWSGS 8 7905 PDH_3271VYDFWSGY 8 7906 PDH_3272 SYDFWSGS 8 7907 PDH_3273 YYDFWSGY 8 4245PDH_3274 LYDFWSGF 8 7908 PDH_3275 HYDFWSGS 8 7909 PDH_3276 SYDFWSGY 87910 PDH_3277 SYDFWSGF 8 7911 PDH_3278 LYDFWSGS 8 7912 PDH_3279 TYDFWSGF8 7913 PDH_3280 LYDFWSGY 8 7914 PDH_3281 AYDFWSGY 8 7915 PDH_3282AYDFWSGS 8 7916 PDH_3283 TYDFWSGS 8 7917 PDH_3284 IYDFWSGY 8 7918PDH_3285 TYDFWSGY 8 7919 PDH_3286 NYDFWSGS 8 7920 PDH_3287 DYDFWSGF 87921 PDH_3288 IYDFWSGS 8 7922 PDH_3289 FYDFWSGF 8 7923 PDH_3290 HDILTGYS8 7924 PDH_3291 PDILTGYY 8 7925 PDH_3292 PDILTGYF 8 7926 PDH_3293TDILTGYS 8 7927 PDH_3294 HDILTGYY 8 7928 PDH_3295 YDILTGYF 8 7929PDH_3296 IDILTGYS 8 7930 PDH_3297 IDILTGYY 8 7931 PDH_3298 IDILTGYF 87932 PDH_3299 LDILTGYF 8 7933 PDH_3300 LDILTGYY 8 7934 PDH_3301 LDILTGYS8 7935 PDH_3302 DDILTGYF 8 7936 PDH_3303 FDILTGYF 8 7937 PDH_3304SDILTGYF 8 7938 PDH_3305 ADILTGYY 8 7939 PDH_3306 VDILTGYY 8 7940PDH_3307 VDILTGYS 8 7941 PDH_3308 ADILTGYS 8 7942 PDH_3309 DDILTGYS 87943 PDH_3310 FDILTGYS 8 7944 PDH_3311 SDILTGYS 8 7945 PDH_3312 DDILTGYY8 7946 PDH_3313 SDILTGYY 8 7947 PDH_3314 YDILTGYY 8 4322 PDH_3315FDILTGYY 8 7948 PDH_3316 HDILTGYF 8 7949 PDH_3317 NDILTGYY 8 7950PDH_3318 NDILTGYF 8 7951 PDH_3319 PDILTGYS 8 7952 PDH_3320 VDILTGYF 87953 PDH_3321 TDILTGYY 8 7954 PDH_3322 TDILTGYF 8 7955 PDH_3323 YDILTGYS8 7956 PDH_3324 NDILTGYS 8 7957 PDH_3325 ADILTGYF 8 7958 PDH_3326SCSGGSCS 8 7959 PDH_3327 HCSGGSCF 8 7960 PDH_3328 DCSGGSCS 8 7961PDH_3329 PCSGGSCF 8 7962 PDH_3330 PCSGGSCY 8 7963 PDH_3331 SCSGGSCY 87964 PDH_3332 PCSGGSCS 8 7965 PDH_3333 LCSGGSCY 8 7966 PDH_3334 ACSGGSCY8 7967 PDH_3335 ACSGGSCS 8 7968 PDH_3336 ACSGGSCF 8 7969 PDH_3337VCSGGSCY 8 7970 PDH_3338 VCSGGSCF 8 7971 PDH_3339 VCSGGSCS 8 7972PDH_3340 YCSGGSCS 8 7973 PDH_3341 YCSGGSCF 8 7974 PDH_3342 DCSGGSCF 87975 PDH_3343 DCSGGSCY 8 7976 PDH_3344 FCSGGSCS 8 7977 PDH_3345 FCSGGSCY8 7978 PDH_3346 FCSGGSCF 8 7979 PDH_3347 SCSGGSCF 8 7980 PDH_3348YCSGGSCY 8 3771 PDH_3349 LCSGGSCF 8 7981 PDH_3350 LCSGGSCS 8 7982PDH_3351 HCSGGSCS 8 7983 PDH_3352 HCSGGSCY 8 7984 PDH_3353 ACSSTSCY 87985 PDH_3354 YCSSTSCF 8 7986 PDH_3355 FCSSTSCY 8 7987 PDH_3356 PCSSTSCF8 7988 PDH_3357 FCSSTSCS 8 7989 PDH_3358 DCSSTSCF 8 7990 PDH_3359VCSSTSCS 8 7991 PDH_3360 VCSSTSCF 8 7992 PDH_3361 LCSSTSCS 8 7993PDH_3362 VCSSTSCY 8 7994 PDH_3363 ACSSTSCS 8 7995 PDH_3364 LCSSTSCY 87996 PDH_3365 LCSSTSCF 8 7997 PDH_3366 FCSSTSCF 8 7998 PDH_3367 HCSSTSCY8 7999 PDH_3368 HCSSTSCS 8 8000 PDH_3369 HCSSTSCF 8 8001 PDH_3370SCSSTSCF 8 8002 PDH_3371 SCSSTSCS 8 8003 PDH_3372 YCSSTSCS 8 8004PDH_3373 DCSSTSCY 8 8005 PDH_3374 DCSSTSCS 8 8006 PDH_3375 SCSSTSCY 88007 PDH_3376 PCSSTSCS 8 8008 PDH_3377 ACSSTSCF 8 8009 PDH_3378 PCSSTSCY8 8010 PDH_3379 YCSSTSCY 8 3810 PDH_3380 LYCGGDCS 8 8011 PDH_3381EYCGGDCS 8 8012 PDH_3382 LYCGGDCY 8 8013 PDH_3383 VYCGGDCS 8 8014PDH_3384 VYCGGDCY 8 8015 PDH_3385 AYCGGDCY 8 3863 PDH_3386 AYCGGDCF 88016 PDH_3387 EYCGGDCY 8 8017 PDH_3388 TYCGGDCF 8 8018 PDH_3389 LYCGGDCF8 8019 PDH_3390 AYCGGDCS 8 8020 PDH_3391 QYCGGDCF 8 8021 PDH_3392QYCGGDCS 8 8022 PDH_3393 KYCGGDCF 8 8023 PDH_3394 KYCGGDCY 8 8024PDH_3395 QYCGGDCY 8 8025 PDH_3396 KYCGGDCS 8 8026 PDH_3397 VYCGGDCF 88027 PDH_3398 IYCGGDCF 8 8028 PDH_3399 IYCGGDCY 8 8029 PDH_3400 IYCGGDCS8 8030 PDH_3401 PYCGGDCS 8 8031 PDH_3402 PYCGGDCF 8 8032 PDH_3403TYCGGDCY 8 8033 PDH_3404 EYCGGDCF 8 8034 PDH_3405 PYCGGDCY 8 8035PDH_3406 TYCGGDCS 8 8036 PDH_3407 DYDSSGYS 8 8037 PDH_3408 YYDSSGYF 88038 PDH_3409 LYDSSGYS 8 8039 PDH_3410 AYDSSGYS 8 8040 PDH_3411 AYDSSGYY8 8041 PDH_3412 NYDSSGYY 8 8042 PDH_3413 IYDSSGYS 8 8043 PDH_3414FYDSSGYF 8 8044 PDH_3415 DYDSSGYY 8 8045 PDH_3416 YYDSSGYS 8 8046PDH_3417 TYDSSGYY 8 8047 PDH_3418 TYDSSGYS 8 8048 PDH_3419 PYDSSGYF 88049 PDH_3420 IYDSSGYY 8 8050 PDH_3421 IYDSSGYF 8 8051 PDH_3422 YYDSSGYY8 4165 PDH_3423 NYDSSGYS 8 8052 PDH_3424 NYDSSGYF 8 8053 PDH_3425VYDSSGYY 8 8054 PDH_3426 VYDSSGYF 8 8055 PDH_3427 FYDSSGYY 8 8056PDH_3428 FYDSSGYS 8 8057 PDH_3429 SYDSSGYY 8 8058 PDH_3430 SYDSSGYS 88059 PDH_3431 PYDSSGYS 8 8060 PDH_3432 PYDSSGYY 8 8061 PDH_3433 LYDSSGYY8 8062 PDH_3434 VYDSSGYS 8 8063 PDH_3435 SYDSSGYF 8 8064 PDH_3436LYDSSGYF 8 8065 PDH_3437 HYDSSGYY 8 8066 PDH_3438 HYDSSGYS 8 8067PDH_3439 TYDSSGYF 8 8068 PDH_3440 AYDSSGYF 8 8069 PDH_3441 DYDSSGYF 88070 PDH_3442 HYDSSGYF 8 8071 PDH_3443 RYYGSGSY 8 8072 PDH_3444 RYYGSGSS8 8073 PDH_3445 GYYGSGSS 8 8074 PDH_3446 GYYGSGSY 8 8075 PDH_3447FDFWSGYS 8 8076 PDH_3448 HDFWSGYF 8 8077 PDH_3449 FDFWSGYY 8 8078PDH_3450 SDFWSGYS 8 8079 PDH_3451 SDFWSGYY 8 8080 PDH_3452 PDFWSGYS 88081 PDH_3453 HDFWSGYS 8 8082 PDH_3454 IDFWSGYY 8 8083 PDH_3455 HDFWSGYY8 8084 PDH_3456 NDFWSGYF 8 8085 PDH_3457 YDFWSGYS 8 8086 PDH_3458IDFWSGYS 8 8087 PDH_3459 PDFWSGYY 8 8088 PDH_3460 SDFWSGYF 8 8089PDH_3461 VDFWSGYS 8 8090 PDH_3462 IDFWSGYF 8 8091 PDH_3463 YDFWSGYY 84246 PDH_3464 YDFWSGYF 8 8092 PDH_3465 TDFWSGYY 8 8093 PDH_3466 DDFWSGYS8 8094 PDH_3467 LDFWSGYS 8 8095 PDH_3468 DDFWSGYY 8 8096 PDH_3469DDFWSGYF 8 8097 PDH_3470 VDFWSGYY 8 8098 PDH_3471 VDFWSGYF 8 8099PDH_3472 NDFWSGYY 8 8100 PDH_3473 FDFWSGYF 8 8101 PDH_3474 NDFWSGYS 88102 PDH_3475 LDFWSGYY 8 8103 PDH_3476 ADFWSGYY 8 8104 PDH_3477 ADFWSGYS8 8105 PDH_3478 TDFWSGYS 8 8106 PDH_3479 TDFWSGYF 8 8107 PDH_3480ADFWSGYF 8 8108 PDH_3481 LDFWSGYF 8 8109 PDH_3482 PDFWSGYF 8 8110PDH_3483 LLRYFDWY 8 8111 PDH_3484 QLRYFDWY 8 8112 PDH_3485 PLRYFDWL 88113 PDH_3486 ILRYFDWF 8 8114 PDH_3487 ILRYFDWY 8 8115 PDH_3488 ALRYFDWL8 8116 PDH_3489 QLRYFDWF 8 8117 PDH_3490 KLRYFDWL 8 8118 PDH_3491TLRYFDWL 8 8119 PDH_3492 TLRYFDWH 8 8120 PDH_3493 ELRYFDWL 8 8121PDH_3494 ELRYFDWH 8 8122 PDH_3495 PLRYFDWH 8 8123 PDH_3496 ELRYFDWY 88124 PDH_3497 ELRYFDWF 8 8125 PDH_3498 LLRYFDWL 8 8126 PDH_3499 VLRYFDWF8 8127 PDH_3500 TLRYFDWF 8 8128 PDH_3501 ALRYFDWF 8 8129 PDH_3502ILRYFDWH 8 8130 PDH_3503 QLRYFDWL 8 8131 PDH_3504 QLRYFDWH 8 8132PDH_3505 VLRYFDWY 8 8133 PDH_3506 TLRYFDWY 8 8134 PDH_3507 ILRYFDWL 88135 PDH_3508 LLRYFDWH 8 8136 PDH_3509 VLRYFDWH 8 8137 PDH_3510 KLRYFDWY8 8138 PDH_3511 KLRYFDWF 8 8139 PDH_3512 ALRYFDWH 8 8140 PDH_3513ALRYFDWY 8 8141 PDH_3514 VLRYFDWL 8 4298 PDH_3515 KLRYFDWH 8 8142PDH_3516 PLRYFDWY 8 8143 PDH_3517 PLRYFDWF 8 8144 PDH_3518 LLRYFDWF 88145 PDH_3519 IYYDSSGYS 9 8146 PDH_3520 TYYDSSGYS 9 8147 PDH_3521HYYDSSGYY 9 8148 PDH_3522 TYYDSSGYY 9 8149 PDH_3523 HYYDSSGYS 9 8150PDH_3524 NYYDSSGYF 9 8151 PDH_3525 AYYDSSGYS 9 8152 PDH_3526 DYYDSSGYF 98153 PDH_3527 DYYDSSGYS 9 8154 PDH_3528 LYYDSSGYS 9 8155 PDH_3529NYYDSSGYS 9 8156 PDH_3530 HYYDSSGYF 9 8157 PDH_3531 DYYDSSGYY 9 8158PDH_3532 LYYDSSGYY 9 8159 PDH_3533 IYYDSSGYY 9 8160 PDH_3534 LYYDSSGYF 98161 PDH_3535 IYYDSSGYF 9 8162 PDH_3536 AYYDSSGYF 9 8163 PDH_3537AYYDSSGYY 9 8164 PDH_3538 FYYDSSGYS 9 8165 PDH_3539 YYYDSSGYS 9 8166PDH_3540 FYYDSSGYY 9 8167 PDH_3541 FYYDSSGYF 9 8168 PDH_3542 YYYDSSGYY 94162 PDH_3543 YYYDSSGYF 9 8169 PDH_3544 VYYDSSGYF 9 8170 PDH_3545PYYDSSGYY 9 8171 PDH_3546 PYYDSSGYS 9 8172 PDH_3547 VYYDSSGYS 9 8173PDH_3548 SYYDSSGYY 9 8174 PDH_3549 NYYDSSGYY 9 8175 PDH_3550 VYYDSSGYY 98176 PDH_3551 SYYDSSGYS 9 8177 PDH_3552 SYYDSSGYF 9 8178 PDH_3553TYYDSSGYF 9 8179 PDH_3554 PYYDSSGYF 9 8180 PDH_3555 PYCSGGSCF 9 8181PDH_3556 TYCSGGSCF 9 8182 PDH_3557 IYCSGGSCF 9 8183 PDH_3558 VYCSGGSCS 98184 PDH_3559 TYCSGGSCY 9 8185 PDH_3560 VYCSGGSCF 9 8186 PDH_3561TYCSGGSCS 9 8187 PDH_3562 GYCSGGSCS 9 8188 PDH_3563 IYCSGGSCS 9 8189PDH_3564 GYCSGGSCY 9 3768 PDH_3565 IYCSGGSCY 9 8190 PDH_3566 VYCSGGSCY 98191 PDH_3567 AYCSGGSCF 9 8192 PDH_3568 GYCSGGSCF 9 8193 PDH_3569LYCSGGSCF 9 8194 PDH_3570 RYCSGGSCF 9 8195 PDH_3571 LYCSGGSCS 9 8196PDH_3572 RYCSGGSCY 9 8197 PDH_3573 AYCSGGSCS 9 8198 PDH_3574 SYCSGGSCY 98199 PDH_3575 SYCSGGSCF 9 8200 PDH_3576 AYCSGGSCY 9 8201 PDH_3577SYCSGGSCS 9 8202 PDH_3578 PYCSGGSCS 9 8203 PDH_3579 LYCSGGSCY 9 8204PDH_3580 RYCSGGSCS 9 8205 PDH_3581 PYCSGGSCY 9 8206 PDH_3582 TYCSSTSCY 98207 PDH_3583 AYCSSTSCY 9 8208 PDH_3584 AYCSSTSCS 9 8209 PDH_3585RYCSSTSCS 9 8210 PDH_3586 TYCSSTSCS 9 8211 PDH_3587 PYCSSTSCY 9 8212PDH_3588 PYCSSTSCS 9 8213 PDH_3589 RYCSSTSCY 9 8214 PDH_3590 VYCSSTSCS 98215 PDH_3591 VYCSSTSCY 9 8216 PDH_3592 LYCSSTSCF 9 8217 PDH_3593LYCSSTSCY 9 8218 PDH_3594 PYCSSTSCF 9 8219 PDH_3595 VYCSSTSCF 9 8220PDH_3596 IYCSSTSCY 9 8221 PDH_3597 IYCSSTSCS 9 8222 PDH_3598 IYCSSTSCF 98223 PDH_3599 SYCSSTSCS 9 8224 PDH_3600 LYCSSTSCS 9 8225 PDH_3601SYCSSTSCY 9 8226 PDH_3602 SYCSSTSCF 9 8227 PDH_3603 GYCSSTSCY 9 3807PDH_3604 GYCSSTSCF 9 8228 PDH_3605 GYCSSTSCS 9 8229 PDH_3606 RYCSSTSCF 98230 PDH_3607 TYCSSTSCF 9 8231 PDH_3608 AYCSSTSCF 9 8232 PDH_3609IYDFWSGYY 9 8233 PDH_3610 NYDFWSGYY 9 8234 PDH_3611 PYDFWSGYF 9 8235PDH_3612 SYDFWSGYF 9 8236 PDH_3613 VYDFWSGYF 9 8237 PDH_3614 VYDFWSGYY 98238 PDH_3615 HYDFWSGYY 9 8239 PDH_3616 HYDFWSGYF 9 8240 PDH_3617HYDFWSGYS 9 8241 PDH_3618 IYDFWSGYS 9 8242 PDH_3619 NYDFWSGYS 9 8243PDH_3620 AYDFWSGYS 9 8244 PDH_3621 SYDFWSGYS 9 8245 PDH_3622 PYDFWSGYS 98246 PDH_3623 AYDFWSGYY 9 8247 PDH_3624 SYDFWSGYY 9 8248 PDH_3625PYDFWSGYY 9 8249 PDH_3626 FYDFWSGYY 9 8250 PDH_3627 FYDFWSGYF 9 8251PDH_3628 DYDFWSGYS 9 8252 PDH_3629 FYDFWSGYS 9 8253 PDH_3630 DYDFWSGYF 98254 PDH_3631 LYDFWSGYY 9 8255 PDH_3632 AYDFWSGYF 9 8256 PDH_3633LYDFWSGYS 9 8257 PDH_3634 DYDFWSGYY 9 8258 PDH_3635 LYDFWSGYF 9 8259PDH_3636 YYDFWSGYY 9 4243 PDH_3637 TYDFWSGYY 9 8260 PDH_3638 TYDFWSGYF 98261 PDH_3639 YYDFWSGYS 9 8262 PDH_3640 YYDFWSGYF 9 8263 PDH_3641TYDFWSGYS 9 8264 PDH_3642 VYDFWSGYS 9 8265 PDH_3643 NYDFWSGYF 9 8266PDH_3644 IYDFWSGYF 9 8267 PDH_3645 FCSGGSCYS 9 8268 PDH_3646 LCSGGSCYS 98269 PDH_3647 VCSGGSCYS 9 8270 PDH_3648 ACSGGSCYS 9 8271 PDH_3649ACSGGSCYY 9 8272 PDH_3650 FCSGGSCYY 9 8273 PDH_3651 LCSGGSCYF 9 8274PDH_3652 LCSGGSCYY 9 8275 PDH_3653 VCSGGSCYY 9 8276 PDH_3654 YCSGGSCYS 93769 PDH_3655 PCSGGSCYS 9 8277 PDH_3656 PCSGGSCYY 9 8278 PDH_3657PCSGGSCYF 9 8279 PDH_3658 YCSGGSCYF 9 8280 PDH_3659 YCSGGSCYY 9 8281PDH_3660 SCSGGSCYY 9 8282 PDH_3661 HCSGGSCYF 9 8283 PDH_3662 DCSGGSCYY 98284 PDH_3663 SCSGGSCYF 9 8285 PDH_3664 DCSGGSCYS 9 8286 PDH_3665HCSGGSCYS 9 8287 PDH_3666 SCSGGSCYS 9 8288 PDH_3667 FCSGGSCYF 9 8289PDH_3668 ACSGGSCYF 9 8290 PDH_3669 DCSGGSCYF 9 8291 PDH_3670 HCSGGSCYY 98292 PDH_3671 VCSGGSCYF 9 8293 PDH_3672 FYYDSSGYYY 10 8294 PDH_3673YYYDSSGYYF 10 8295 PDH_3674 PYYDSSGYYY 10 8296 PDH_3675 VYYDSSGYYS 108297 PDH_3676 PYYDSSGYYS 10 8298 PDH_3677 FYYDSSGYYS 10 8299 PDH_3678NYYDSSGYYY 10 8300 PDH_3679 NYYDSSGYYS 10 8301 PDH_3680 DYYDSSGYYS 108302 PDH_3681 HYYDSSGYYF 10 8303 PDH_3682 DYYDSSGYYY 10 8304 PDH_3683NYYDSSGYYF 10 8305 PDH_3684 HYYDSSGYYY 10 8306 PDH_3685 LYYDSSGYYF 108307 PDH_3686 IYYDSSGYYS 10 8308 PDH_3687 YYYDSSGYYS 10 8309 PDH_3688IYYDSSGYYF 10 8310 PDH_3689 YYYDSSGYYY 10 4161 PDH_3690 HYYDSSGYYS 108311 PDH_3691 TYYDSSGYYS 10 8312 PDH_3692 IYYDSSGYYY 10 8313 PDH_3693TYYDSSGYYF 10 8314 PDH_3694 LYYDSSGYYS 10 8315 PDH_3695 TYYDSSGYYY 108316 PDH_3696 LYYDSSGYYY 10 8317 PDH_3697 AYYDSSGYYF 10 8318 PDH_3698FYYDSSGYYF 10 8319 PDH_3699 AYYDSSGYYY 10 8320 PDH_3700 VYYDSSGYYY 108321 PDH_3701 SYYDSSGYYF 10 8322 PDH_3702 PYYDSSGYYF 10 8323 PDH_3703SYYDSSGYYY 10 8324 PDH_3704 AYYDSSGYYS 10 8325 PDH_3705 DYYDSSGYYF 108326 PDH_3706 SYYDSSGYYS 10 8327 PDH_3707 VYYDSSGYYF 10 8328 PDH_3708IYDYVWGSYAS 11 8329 PDH_3709 AYDYVWGSYAS 11 8330 PDH_3710 IYDYVWGSYAY 118331 PDH_3711 NYDYVWGSYAY 11 8332 PDH_3712 NYDYVWGSYAS 11 8333 PDH_3713YYDYVWGSYAF 11 8334 PDH_3714 DYDYVWGSYAF 11 8335 PDH_3715 SYDYVWGSYAY 118336 PDH_3716 DYDYVWGSYAS 11 8337 PDH_3717 DYDYVWGSYAY 11 8338 PDH_3718FYDYVWGSYAS 11 8339 PDH_3719 NYDYVWGSYAF 11 8340 PDH_3720 YYDYVWGSYAS 118341 PDH_3721 FYDYVWGSYAY 11 8342 PDH_3722 SYDYVWGSYAS 11 8343 PDH_3723PYDYVWGSYAF 11 8344 PDH_3724 TYDYVWGSYAY 11 8345 PDH_3725 VYDYVWGSYAY 118346 PDH_3726 SYDYVWGSYAF 11 8347 PDH_3727 FYDYVWGSYAF 11 8348 PDH_3728HYDYVWGSYAS 11 8349 PDH_3729 VYDYVWGSYAS 11 8350 PDH_3730 VYDYVWGSYAF 118351 PDH_3731 YYDYVWGSYAY 11 4071 PDH_3732 AYDYVWGSYAY 11 8352 PDH_3733LYDYVWGSYAS 11 8353 PDH_3734 TYDYVWGSYAF 11 8354 PDH_3735 AYDYVWGSYAF 118355 PDH_3736 HYDYVWGSYAY 11 8356 PDH_3737 TYDYVWGSYAS 11 8357 PDH_3738LYDYVWGSYAF 11 8358 PDH_3739 PYDYVWGSYAY 11 8359 PDH_3740 PYDYVWGSYAS 118360 PDH_3741 HYDYVWGSYAF 11 8361 PDH_3742 LYDYVWGSYAY 11 8362 PDH_3743IYDYVWGSYAF 11 8363 PDH_3744 NYDYVWGSYAYT 12 8364 PDH_3745 NYDYVWGSYAYI12 8365 PDH_3746 IYDYVWGSYAYI 12 8366 PDH_3747 YYDYVWGSYAYK 12 8367PDH_3748 NYDYVWGSYAYK 12 8368 PDH_3749 YYDYVWGSYAYT 12 4070 PDH_3750PYDYVWGSYAYT 12 8369 PDH_3751 DYDYVWGSYAYI 12 8370 PDH_3752 PYDYVWGSYAYK12 8371 PDH_3753 FYDYVWGSYAYI 12 8372 PDH_3754 VYDYVWGSYAYT 12 8373PDH_3755 DYDYVWGSYAYK 12 8374 PDH_3756 IYDYVWGSYAYT 12 8375 PDH_3757IYDYVWGSYAYK 12 8376 PDH_3758 LYDYVWGSYAYI 12 8377 PDH_3759 HYDYVWGSYAYK12 8378 PDH_3760 TYDYVWGSYAYI 12 8379 PDH_3761 HYDYVWGSYAYT 12 8380PDH_3762 AYDYVWGSYAYT 12 8381 PDH_3763 AYDYVWGSYAYK 12 8382 PDH_3764AYDYVWGSYAYI 12 8383 PDH_3765 TYDYVWGSYAYK 12 8384 PDH_3766 DYDYVWGSYAYT12 8385 PDH_3767 VYDYVWGSYAYK 12 8386 PDH_3768 TYDYVWGSYAYT 12 8387PDH_3769 FYDYVWGSYAYK 12 8388 PDH_3770 LYDYVWGSYAYK 12 8389 PDH_3771VYDYVWGSYAYI 12 8390 PDH_3772 LYDYVWGSYAYT 12 8391 PDH_3773 PYDYVWGSYAYI12 8392 PDH_3774 FYDYVWGSYAYT 12 8393 PDH_3775 YYDYVWGSYAYI 12 8394PDH_3776 SYDYVWGSYAYI 12 8395 PDH_3777 HYDYVWGSYAYI 12 8396 PDH_3778SYDYVWGSYAYT 12 8397 PDH_3779 SYDYVWGSYAYK 12 8398

TABLE 29 Theoretical segment pool of oligonucleotide sequences encodingN2 segments of Example 14. Degenerate Peptide SEQ ID Name Oligo LengthNO N2_000 0 Not degenerate n/a N2_001 GCT 1 Not degenerate n/a N2_002GAT 1 Not degenerate n/a N2_003 GAG 1 Not degenerate n/a N2_004 TTT 1Not degenerate n/a N2_005 GGC 1 Not degenerate n/a N2_006 CAT 1 Notdegenerate n/a N2_007 ATC 1 Not degenerate n/a N2_008 AAA 1 Notdegenerate n/a N2_009 TTG 1 Not degenerate n/a N2_010 ATG 1 Notdegenerate n/a N2_011 CCT 1 Not degenerate n/a N2_012 CAA 1 Notdegenerate n/a N2_013 AGG 1 Not degenerate n/a N2_014 TCA 1 Notdegenerate n/a N2_015 ACC 1 Not degenerate n/a N2_016 GTT 1 Notdegenerate n/a N2_017 TGG 1 Not degenerate n/a N2_018 TAC 1 Notdegenerate n/a N2_019 GMCKHT 2 n/a N2_020 GMCSVT 2 n/a N2_021 GMCSHT 2n/a N2_022 GMCSVG 2 n/a N2_023 GMTDYT 2 n/a N2_024 KHCGAS 2 n/a N2_025KHCGRC 2 n/a N2_026 KHCGWG 2 n/a N2_027 KHTTTM 2 n/a N2_028 KHTTYC 2 n/aN2_029 KHTTWC 2 n/a N2_030 KHCRGA 2 n/a N2_031 KHCKGG 2 n/a N2_032KHCCWC 2 n/a N2_033 KHCCMT 2 n/a N2_034 KHCMCA 2 n/a N2_035 GVCSWG 2 n/aN2_036 GVCMKC 2 n/a N2_037 GVCWSG 2 n/a N2_038 SVCYAC 2 n/a N2_039GNAAHA 2 n/a N2_040 BYCSAG 2 n/a N2_041 RBAAWA 2 n/a N2_042 RBAAYA 2 n/aN2_043 SBAMAA 2 n/a N2_044 VSCMAA 2 n/a N2_045 GRARVG 2 n/a N2_046GRADYT 2 n/a N2_047 GRABYT 2 n/a N2_048 GRAKBG 2 n/a N2_049 RDAGMT 2 n/aN2_050 RDAGAK 2 n/a N2_051 RDAGRT 2 n/a N2_052 RDAGWG 2 n/a N2_053RDARGG 2 n/a N2_054 RDACYA 2 n/a N2_055 SDACSA 2 n/a N2_056 VWACYA 2 n/aN2_057 VWATYA 2 n/a N2_058 VWAASA 2 n/a N2_059 YHCGMC 2 n/a N2_060YHCGMG 2 n/a N2_061 YHCGST 2 n/a N2_062 YHCSCG 2 n/a N2_063 YHCKCG 2 n/aN2_064 YHCSAC 2 n/a N2_065 YHCKAC 2 n/a N2_066 YHCRGA 2 n/a N2_067YHCCWC 2 n/a N2_068 YHCMCA 2 n/a N2_069 WTCYHT 2 n/a N2_070 HYCGWG 2 n/aN2_071 HYCTTM 2 n/a N2_072 HYCAGM 2 n/a N2_073 HYCTMC 2 n/a N2_074VKCTWT 2 n/a N2_075 CNCVGC 2 n/a N2_076 MHAGAK 2 n/a N2_077 MHAGRC 2 n/aN2_078 MHAGWG 2 n/a N2_079 MHAMCA 2 n/a N2_080 ANAGBT 2 n/a N2_081MBCYAC 2 n/a N2_082 MBCAWA 2 n/a N2_083 MHGGKA 2 n/a N2_084 CNABTT 2 n/aN2_085 CVACNA 2 n/a N2_086 CVAYSG 2 n/a N2_087 MSCAHG 2 n/a N2_088CRAKBG 2 n/a N2_089 WSGHCA 2 n/a N2_090 WGGKHC 2 n/a N2_091 MBCATR 2 n/aN2_092 AYABSG 2 n/a N2_093 VYCAWG 2 n/a N2_094 BGGSAK 2 n/a N2_095AHGRYT 2 n/a N2_096 BWCAMA 2 n/a N2_097 BHCTGG 2 n/a N2_098 TGGBHC 2 n/aN2_099 TGGVBT 2 n/a N2_100 NHCGCAGCC 3 n/a N2_101 BHCGGAATG 3 n/a N2_102BHCGGAGGA 3 n/a N2_103 BHCGGAGTA 3 n/a N2_104 VNCGCAGGA 3 n/a N2_105VBCGGAGCC 3 n/a N2_106 VBCGGAGGA 3 n/a N2_107 VBCGGACTA 3 n/a N2_108VBCGGAAGG 3 n/a N2_109 VBCGGAAGC 3 n/a N2_110 VBCGGAGTA 3 n/a N2_111VNCCTTGGA 3 n/a N2_112 VNCCCAGGA 3 n/a N2_113 VNCCCACCA 3 n/a N2_114VNCAGAGGA 3 n/a N2_115 VNCAGCGGA 3 n/a N2_116 VBCACAGGA 3 n/a N2_117VNCGTAGGA 3 n/a N2_118 BHCGGACAC 3 n/a N2_119 NHCAAACAA 3 n/a N2_120NHCAAAAGA 3 n/a N2_121 BHCACACAA 3 n/a N2_122 VNCTTTGAG 3 n/a N2_123VNCCCACTA 3 n/a N2_124 VNCCCATAC 3 n/a N2_125 BHCGGAGAG 3 n/a N2_126BHCGGACTA 3 n/a N2_127 BHCGGATGG 3 n/a N2_128 BHCGGATAC 3 n/a N2_129NHCAGAGGA 3 n/a N2_130 NHCAGCGAG 3 n/a N2_131 NHCAGCTGG 3 n/a N2_132VHAGGAGGA 3 n/a N2_133 BHCGGAAGG 3 n/a N2_134 NHCCAAGGA 3 n/a N2_135BHCACAGCT 3 n/a N2_136 GGABHCGGATAC 4 8399 N2_137 AGABHCGGATAC 4 8400N2_138 AGCBHCGGATAC 4 8401 N2_139 CCABHCGGATAC 4 8402 N2_140GGTAGAVHGTAC 4 8403 N2_141 AGGAGAVHGTAC 4 8404 N2_142 GGABHCGGATGG 48405 N2_143 GGABHCGGACTA 4 8406 N2_144 GGABHCACAGCT 4 8407 N2_145GGABHCACACAA 4 8408

TABLE 30 Theoretical segment pool of unique N2 polypeptide segmentsencoded by the oligonucleotides of Table 29. Name Sequence Length SEQ IDNO PN2_000 0 #N/A PN2_001 A 1 #N/A PN2_002 D 1 #N/A PN2_003 E 1 #N/APN2_004 F 1 #N/A PN2_005 G 1 #N/A PN2_006 H 1 #N/A PN2_007 I 1 #N/APN2_008 K 1 #N/A PN2_009 L 1 #N/A PN2_010 M 1 #N/A PN2_011 P 1 #N/APN2_012 Q 1 #N/A PN2_013 R 1 #N/A PN2_014 S 1 #N/A PN2_015 T 1 #N/APN2_016 V 1 #N/A PN2_017 W 1 #N/A PN2_018 Y 1 #N/A PN2_019 GW 2 #N/APN2_020 GV 2 #N/A PN2_021 GT 2 #N/A PN2_022 GS 2 #N/A PN2_023 GR 2 #N/APN2_024 GQ 2 #N/A PN2_025 GP 2 #N/A PN2_026 GY 2 #N/A PN2_027 GG 2 #N/APN2_028 GF 2 #N/A PN2_029 GE 2 #N/A PN2_030 GD 2 #N/A PN2_031 GA 2 #N/APN2_032 GL 2 #N/A PN2_033 GK 2 #N/A PN2_034 GI 2 #N/A PN2_035 GH 2 #N/APN2_036 MG 2 #N/A PN2_037 MA 2 #N/A PN2_038 MI 2 #N/A PN2_039 MT 2 #N/APN2_040 MV 2 #N/A PN2_041 FP 2 #N/A PN2_042 FQ 2 #N/A PN2_043 FR 2 #N/APN2_044 FS 2 #N/A PN2_045 FT 2 #N/A PN2_046 FV 2 #N/A PN2_047 FW 2 #N/APN2_048 FY 2 #N/A PN2_049 FA 2 #N/A PN2_050 FD 2 #N/A PN2_051 FE 2 #N/APN2_052 FF 2 #N/A PN2_053 FG 2 #N/A PN2_054 FH 2 #N/A PN2_055 FK 2 #N/APN2_056 FL 2 #N/A PN2_057 SY 2 #N/A PN2_058 SS 2 #N/A PN2_059 SR 2 #N/APN2_060 SQ 2 #N/A PN2_061 SP 2 #N/A PN2_062 SW 2 #N/A PN2_063 SV 2 #N/APN2_064 ST 2 #N/A PN2_065 SK 2 #N/A PN2_066 SI 2 #N/A PN2_067 SH 2 #N/APN2_068 SM 2 #N/A PN2_069 SL 2 #N/A PN2_070 SA 2 #N/A PN2_071 SG 2 #N/APN2_072 SF 2 #N/A PN2_073 SE 2 #N/A PN2_074 SD 2 #N/A PN2_075 YH 2 #N/APN2_076 YK 2 #N/A PN2_077 YL 2 #N/A PN2_078 YA 2 #N/A PN2_079 YE 2 #N/APN2_080 YD 2 #N/A PN2_081 YG 2 #N/A PN2_082 YF 2 #N/A PN2_083 YY 2 #N/APN2_084 YP 2 #N/A PN2_085 YS 2 #N/A PN2_086 YR 2 #N/A PN2_087 YT 2 #N/APN2_088 YW 2 #N/A PN2_089 YV 2 #N/A PN2_090 LF 2 #N/A PN2_091 LD 2 #N/APN2_092 LE 2 #N/A PN2_093 LL 2 #N/A PN2_094 LM 2 #N/A PN2_095 LK 2 #N/APN2_096 LH 2 #N/A PN2_097 LI 2 #N/A PN2_098 LW 2 #N/A PN2_099 LT 2 #N/APN2_100 LR 2 #N/A PN2_101 LS 2 #N/A PN2_102 LP 2 #N/A PN2_103 LQ 2 #N/APN2_104 LY 2 #N/A PN2_105 LG 2 #N/A PN2_106 LA 2 #N/A PN2_107 RT 2 #N/APN2_108 RV 2 #N/A PN2_109 RW 2 #N/A PN2_110 RP 2 #N/A PN2_111 RQ 2 #N/APN2_112 RR 2 #N/A PN2_113 RS 2 #N/A PN2_114 RY 2 #N/A PN2_115 RD 2 #N/APN2_116 RE 2 #N/A PN2_117 RF 2 #N/A PN2_118 RG 2 #N/A PN2_119 RA 2 #N/APN2_120 RL 2 #N/A PN2_121 RM 2 #N/A PN2_122 RH 2 #N/A PN2_123 RI 2 #N/APN2_124 RK 2 #N/A PN2_125 LV 2 #N/A PN2_126 IP 2 #N/A PN2_127 EL 2 #N/APN2_128 VK 2 #N/A PN2_129 EI 2 #N/A PN2_130 EK 2 #N/A PN2_131 EE 2 #N/APN2_132 ED 2 #N/A PN2_133 EG 2 #N/A PN2_134 EF 2 #N/A PN2_135 EA 2 #N/APN2_136 IT 2 #N/A PN2_137 ET 2 #N/A PN2_138 EW 2 #N/A PN2_139 EV 2 #N/APN2_140 EP 2 #N/A PN2_141 ES 2 #N/A PN2_142 ER 2 #N/A PN2_143 II 2 #N/APN2_144 IH 2 #N/A PN2_145 VR 2 #N/A PN2_146 VT 2 #N/A PN2_147 KA 2 #N/APN2_148 KG 2 #N/A PN2_149 KE 2 #N/A PN2_150 KD 2 #N/A PN2_151 KI 2 #N/APN2_152 KL 2 #N/A PN2_153 KS 2 #N/A PN2_154 KR 2 #N/A PN2_155 KP 2 #N/APN2_156 KV 2 #N/A PN2_157 KT 2 #N/A PN2_158 DK 2 #N/A PN2_159 DH 2 #N/APN2_160 DI 2 #N/A PN2_161 DF 2 #N/A PN2_162 DG 2 #N/A PN2_163 DD 2 #N/APN2_164 DE 2 #N/A PN2_165 DA 2 #N/A PN2_166 DY 2 #N/A PN2_167 DV 2 #N/APN2_168 DW 2 #N/A PN2_169 DT 2 #N/A PN2_170 DR 2 #N/A PN2_171 DS 2 #N/APN2_172 DP 2 #N/A PN2_173 DQ 2 #N/A PN2_174 QQ 2 #N/A PN2_175 QP 2 #N/APN2_176 QS 2 #N/A PN2_177 QR 2 #N/A PN2_178 QT 2 #N/A PN2_179 QW 2 #N/APN2_180 QA 2 #N/A PN2_181 QE 2 #N/A PN2_182 QD 2 #N/A PN2_183 QG 2 #N/APN2_184 QF 2 #N/A PN2_185 QL 2 #N/A PN2_186 WG 2 #N/A PN2_187 WF 2 #N/APN2_188 WE 2 #N/A PN2_189 WD 2 #N/A PN2_190 WA 2 #N/A PN2_191 WL 2 #N/APN2_192 WI 2 #N/A PN2_193 WH 2 #N/A PN2_194 WV 2 #N/A PN2_195 WT 2 #N/APN2_196 WS 2 #N/A PN2_197 WR 2 #N/A PN2_198 WQ 2 #N/A PN2_199 WP 2 #N/APN2_200 WY 2 #N/A PN2_201 PR 2 #N/A PN2_202 PS 2 #N/A PN2_203 PP 2 #N/APN2_204 PQ 2 #N/A PN2_205 PV 2 #N/A PN2_206 PW 2 #N/A PN2_207 PT 2 #N/APN2_208 PY 2 #N/A PN2_209 PA 2 #N/A PN2_210 PF 2 #N/A PN2_211 PG 2 #N/APN2_212 PD 2 #N/A PN2_213 PE 2 #N/A PN2_214 PK 2 #N/A PN2_215 PH 2 #N/APN2_216 PI 2 #N/A PN2_217 PL 2 #N/A PN2_218 PM 2 #N/A PN2_219 DL 2 #N/APN2_220 IY 2 #N/A PN2_221 VA 2 #N/A PN2_222 VD 2 #N/A PN2_223 VE 2 #N/APN2_224 VF 2 #N/A PN2_225 VG 2 #N/A PN2_226 VH 2 #N/A PN2_227 VI 2 #N/APN2_228 IS 2 #N/A PN2_229 IR 2 #N/A PN2_230 VL 2 #N/A PN2_231 VM 2 #N/APN2_232 IW 2 #N/A PN2_233 IV 2 #N/A PN2_234 VP 2 #N/A PN2_235 VQ 2 #N/APN2_236 IK 2 #N/A PN2_237 VS 2 #N/A PN2_238 IM 2 #N/A PN2_239 IL 2 #N/APN2_240 VV 2 #N/A PN2_241 VW 2 #N/A PN2_242 IA 2 #N/A PN2_243 VY 2 #N/APN2_244 IE 2 #N/A PN2_245 ID 2 #N/A PN2_246 IG 2 #N/A PN2_247 IF 2 #N/APN2_248 TQ 2 #N/A PN2_249 TF 2 #N/A PN2_250 HY 2 #N/A PN2_251 HR 2 #N/APN2_252 HS 2 #N/A PN2_253 HP 2 #N/A PN2_254 HW 2 #N/A PN2_255 HT 2 #N/APN2_256 HK 2 #N/A PN2_257 HH 2 #N/A PN2_258 HL 2 #N/A PN2_259 HA 2 #N/APN2_260 HG 2 #N/A PN2_261 HD 2 #N/A PN2_262 HE 2 #N/A PN2_263 QV 2 #N/APN2_264 TY 2 #N/A PN2_265 TV 2 #N/A PN2_266 TW 2 #N/A PN2_267 TT 2 #N/APN2_268 TR 2 #N/A PN2_269 TS 2 #N/A PN2_270 TP 2 #N/A PN2_271 TL 2 #N/APN2_272 TM 2 #N/A PN2_273 TK 2 #N/A PN2_274 TH 2 #N/A PN2_275 TI 2 #N/APN2_276 TG 2 #N/A PN2_277 TD 2 #N/A PN2_278 TE 2 #N/A PN2_279 TA 2 #N/APN2_280 AA 2 #N/A PN2_281 AE 2 #N/A PN2_282 AD 2 #N/A PN2_283 AG 2 #N/APN2_284 AF 2 #N/A PN2_285 AI 2 #N/A PN2_286 AH 2 #N/A PN2_287 AK 2 #N/APN2_288 AM 2 #N/A PN2_289 AL 2 #N/A PN2_290 AQ 2 #N/A PN2_291 AP 2 #N/APN2_292 AS 2 #N/A PN2_293 AR 2 #N/A PN2_294 AT 2 #N/A PN2_295 AW 2 #N/APN2_296 AV 2 #N/A PN2_297 AY 2 #N/A PN2_298 AGM 3 #N/A PN2_299 AGL 3#N/A PN2_300 AGH 3 #N/A PN2_301 AGG 3 #N/A PN2_302 AGE 3 #N/A PN2_303AGA 3 #N/A PN2_304 TQG 3 #N/A PN2_305 AGY 3 #N/A PN2_306 AGW 3 #N/APN2_307 AGV 3 #N/A PN2_308 AGS 3 #N/A PN2_309 AGR 3 #N/A PN2_310 SPY 3#N/A PN2_311 SPP 3 #N/A PN2_312 TAG 3 #N/A PN2_313 SPL 3 #N/A PN2_314TAA 3 #N/A PN2_315 SPG 3 #N/A PN2_316 VAA 3 #N/A PN2_317 VAG 3 #N/APN2_318 NPL 3 #N/A PN2_319 PAA 3 #N/A PN2_320 NPG 3 #N/A PN2_321 LAG 3#N/A PN2_322 LAA 3 #N/A PN2_323 NPY 3 #N/A PN2_324 ALG 3 #N/A PN2_325RLG 3 #N/A PN2_326 TSG 3 #N/A PN2_327 DTA 3 #N/A PN2_328 PAG 3 #N/APN2_329 QGG 3 #N/A PN2_330 DLG 3 #N/A PN2_331 DTQ 3 #N/A PN2_332 LSG 3#N/A PN2_333 LSE 3 #N/A PN2_334 PGR 3 #N/A PN2_335 VLG 3 #N/A PN2_336LSW 3 #N/A PN2_337 AFE 3 #N/A PN2_338 TTG 3 #N/A PN2_339 FTQ 3 #N/APN2_340 IGV 3 #N/A PN2_341 NFE 3 #N/A PN2_342 IGS 3 #N/A PN2_343 IGR 3#N/A PN2_344 PGS 3 #N/A PN2_345 IGG 3 #N/A PN2_346 GRG 3 #N/A PN2_347IGA 3 #N/A PN2_348 DSG 3 #N/A PN2_349 IGL 3 #N/A PN2_350 HLG 3 #N/APN2_351 HPP 3 #N/A PN2_352 AKQ 3 #N/A PN2_353 GLG 3 #N/A PN2_354 LFE 3#N/A PN2_355 GGV 3 #N/A PN2_356 GGS 3 #N/A PN2_357 GGR 3 #N/A PN2_358GGG 3 #N/A PN2_359 GGA 3 #N/A PN2_360 GGL 3 #N/A PN2_361 YTA 3 #N/APN2_362 LTQ 3 #N/A PN2_363 FQG 3 #N/A PN2_364 LVG 3 #N/A PN2_365 LTA 3#N/A PN2_366 LTG 3 #N/A PN2_367 DPY 3 #N/A PN2_368 DFE 3 #N/A PN2_369DPG 3 #N/A PN2_370 HTA 3 #N/A PN2_371 DPP 3 #N/A PN2_372 PGG 3 #N/APN2_373 VPP 3 #N/A PN2_374 PGA 3 #N/A PN2_375 VPY 3 #N/A PN2_376 PGV 3#N/A PN2_377 VPL 3 #N/A PN2_378 STG 3 #N/A PN2_379 STA 3 #N/A PN2_380HGV 3 #N/A PN2_381 HGW 3 #N/A PN2_382 HGR 3 #N/A PN2_383 HGL 3 #N/APN2_384 HGM 3 #N/A PN2_385 STQ 3 #N/A PN2_386 HGH 3 #N/A PN2_387 HGG 3#N/A PN2_388 DSW 3 #N/A PN2_389 HGE 3 #N/A PN2_390 TFE 3 #N/A PN2_391PGY 3 #N/A PN2_392 PGL 3 #N/A PN2_393 PGM 3 #N/A PN2_394 DKR 3 #N/APN2_395 DKQ 3 #N/A PN2_396 VFE 3 #N/A PN2_397 PRG 3 #N/A PN2_398 PGH 3#N/A PN2_399 PPL 3 #N/A PN2_400 EGG 3 #N/A PN2_401 RAG 3 #N/A PN2_402SAA 3 #N/A PN2_403 FGW 3 #N/A PN2_404 SAG 3 #N/A PN2_405 FGR 3 #N/APN2_406 FGH 3 #N/A PN2_407 FGG 3 #N/A PN2_408 TPG 3 #N/A PN2_409 LPY 3#N/A PN2_410 TPL 3 #N/A PN2_411 LPP 3 #N/A PN2_412 LPL 3 #N/A PN2_413TPP 3 #N/A PN2_414 LPG 3 #N/A PN2_415 HRG 3 #N/A PN2_416 TPY 3 #N/APN2_417 APY 3 #N/A PN2_418 IPG 3 #N/A PN2_419 APP 3 #N/A PN2_420 PQG 3#N/A PN2_421 IPL 3 #N/A PN2_422 IPP 3 #N/A PN2_423 APL 3 #N/A PN2_424SFE 3 #N/A PN2_425 APG 3 #N/A PN2_426 YSE 3 #N/A PN2_427 IFE 3 #N/APN2_428 YSW 3 #N/A PN2_429 PKR 3 #N/A PN2_430 RTG 3 #N/A PN2_431 PKQ 3#N/A PN2_432 HGY 3 #N/A PN2_433 TKR 3 #N/A PN2_434 NLG 3 #N/A PN2_435VKR 3 #N/A PN2_436 RFE 3 #N/A PN2_437 SSW 3 #N/A PN2_438 NPP 3 #N/APN2_439 SSE 3 #N/A PN2_440 SSG 3 #N/A PN2_441 YGR 3 #N/A PN2_442 ATG 3#N/A PN2_443 ATA 3 #N/A PN2_444 HPL 3 #N/A PN2_445 ISW 3 #N/A PN2_446ATQ 3 #N/A PN2_447 ISG 3 #N/A PN2_448 ISE 3 #N/A PN2_449 DGR 3 #N/APN2_450 ASW 3 #N/A PN2_451 DGG 3 #N/A PN2_452 DGE 3 #N/A PN2_453 HPG 3#N/A PN2_454 DGH 3 #N/A PN2_455 DGL 3 #N/A PN2_456 DGM 3 #N/A PN2_457LKQ 3 #N/A PN2_458 DGV 3 #N/A PN2_459 DGW 3 #N/A PN2_460 PVG 3 #N/APN2_461 ASG 3 #N/A PN2_462 IRG 3 #N/A PN2_463 VTA 3 #N/A PN2_464 TSE 3#N/A PN2_465 FRG 3 #N/A PN2_466 ASE 3 #N/A PN2_467 VTG 3 #N/A PN2_468GTG 3 #N/A PN2_469 LGW 3 #N/A PN2_470 VTQ 3 #N/A PN2_471 TLG 3 #N/APN2_472 YAA 3 #N/A PN2_473 DGY 3 #N/A PN2_474 ITG 3 #N/A PN2_475 HVG 3#N/A PN2_476 RPP 3 #N/A PN2_477 AAG 3 #N/A PN2_478 RSG 3 #N/A PN2_479AAA 3 #N/A PN2_480 TGR 3 #N/A PN2_481 TGS 3 #N/A PN2_482 TGV 3 #N/APN2_483 RPY 3 #N/A PN2_484 TGA 3 #N/A PN2_485 TGG 3 #N/A PN2_486 RPG 3#N/A PN2_487 RPL 3 #N/A PN2_488 TGL 3 #N/A PN2_489 FKQ 3 #N/A PN2_490FKR 3 #N/A PN2_491 SLG 3 #N/A PN2_492 LGM 3 #N/A PN2_493 LGA 3 #N/APN2_494 NRG 3 #N/A PN2_495 LGG 3 #N/A PN2_496 LGE 3 #N/A PN2_497 LGY 3#N/A PN2_498 LGR 3 #N/A PN2_499 LGS 3 #N/A PN2_500 LGV 3 #N/A PN2_501GFE 3 #N/A PN2_502 LQG 3 #N/A PN2_503 HSG 3 #N/A PN2_504 HSE 3 #N/APN2_505 HSW 3 #N/A PN2_506 DPL 3 #N/A PN2_507 GPL 3 #N/A PN2_508 HAA 3#N/A PN2_509 IAG 3 #N/A PN2_510 GPG 3 #N/A PN2_511 IAA 3 #N/A PN2_512HAG 3 #N/A PN2_513 DQG 3 #N/A PN2_514 GPY 3 #N/A PN2_515 GPP 3 #N/APN2_516 VQG 3 #N/A PN2_517 RGR 3 #N/A PN2_518 RGS 3 #N/A PN2_519 SKQ 3#N/A PN2_520 RGV 3 #N/A PN2_521 SKR 3 #N/A PN2_522 RGA 3 #N/A PN2_523RGG 3 #N/A PN2_524 RGL 3 #N/A PN2_525 VGL 3 #N/A PN2_526 VGM 3 #N/APN2_527 VGH 3 #N/A PN2_528 FGY 3 #N/A PN2_529 VGE 3 #N/A PN2_530 VGG 3#N/A PN2_531 VGA 3 #N/A PN2_532 YKR 3 #N/A PN2_533 YKQ 3 #N/A PN2_534VGY 3 #N/A PN2_535 VGV 3 #N/A PN2_536 VGW 3 #N/A PN2_537 VGR 3 #N/APN2_538 VGS 3 #N/A PN2_539 FTA 3 #N/A PN2_540 PSG 3 #N/A PN2_541 PSE 3#N/A PN2_542 NVG 3 #N/A PN2_543 FGV 3 #N/A PN2_544 FGL 3 #N/A PN2_545ILG 3 #N/A PN2_546 PGW 3 #N/A PN2_547 FSE 3 #N/A PN2_548 DVG 3 #N/APN2_549 FSW 3 #N/A PN2_550 IKR 3 #N/A PN2_551 IKQ 3 #N/A PN2_552 DSE 3#N/A PN2_553 FGM 3 #N/A PN2_554 VRG 3 #N/A PN2_555 NKQ 3 #N/A PN2_556TVG 3 #N/A PN2_557 NQG 3 #N/A PN2_558 SRG 3 #N/A PN2_559 YRG 3 #N/APN2_560 FGE 3 #N/A PN2_561 PLG 3 #N/A PN2_562 PPG 3 #N/A PN2_563 NSE 3#N/A PN2_564 NSG 3 #N/A PN2_565 PPP 3 #N/A PN2_566 GAG 3 #N/A PN2_567PPY 3 #N/A PN2_568 FAA 3 #N/A PN2_569 NSW 3 #N/A PN2_570 HPY 3 #N/APN2_571 PSW 3 #N/A PN2_572 ARG 3 #N/A PN2_573 SGS 3 #N/A PN2_574 NAA 3#N/A PN2_575 NAG 3 #N/A PN2_576 SGW 3 #N/A PN2_577 GSG 3 #N/A PN2_578DRG 3 #N/A PN2_579 RVG 3 #N/A PN2_580 HKR 3 #N/A PN2_581 HKQ 3 #N/APN2_582 AQG 3 #N/A PN2_583 VPG 3 #N/A PN2_584 AKR 3 #N/A PN2_585 VVG 3#N/A PN2_586 SGL 3 #N/A PN2_587 VKQ 3 #N/A PN2_588 SVG 3 #N/A PN2_589SQG 3 #N/A PN2_590 LKR 3 #N/A PN2_591 PTG 3 #N/A PN2_592 PGE 3 #N/APN2_593 PTA 3 #N/A PN2_594 LLG 3 #N/A PN2_595 PTQ 3 #N/A PN2_596 TRG 3#N/A PN2_597 GVG 3 #N/A PN2_598 IVG 3 #N/A PN2_599 LRG 3 #N/A PN2_600HTQ 3 #N/A PN2_601 AVG 3 #N/A PN2_602 IPY 3 #N/A PN2_603 YQG 3 #N/APN2_604 HFE 3 #N/A PN2_605 RRG 3 #N/A PN2_606 LGH 3 #N/A PN2_607 LGL 3#N/A PN2_608 TSW 3 #N/A PN2_609 TKQ 3 #N/A PN2_610 IQG 3 #N/A PN2_611HQG 3 #N/A PN2_612 SGY 3 #N/A PN2_613 DAG 3 #N/A PN2_614 DAA 3 #N/APN2_615 SGR 3 #N/A PN2_616 SGV 3 #N/A PN2_617 SGH 3 #N/A PN2_618 SGM 3#N/A PN2_619 SGA 3 #N/A PN2_620 SGE 3 #N/A PN2_621 SGG 3 #N/A PN2_622YTQ 3 #N/A PN2_623 YGG 3 #N/A PN2_624 YGE 3 #N/A PN2_625 PFE 3 #N/APN2_626 VSW 3 #N/A PN2_627 YGM 3 #N/A PN2_628 YGL 3 #N/A PN2_629 YGH 3#N/A PN2_630 YGW 3 #N/A PN2_631 YGV 3 #N/A PN2_632 NKR 3 #N/A PN2_633VSE 3 #N/A PN2_634 KGG 3 #N/A PN2_635 VSG 3 #N/A PN2_636 YGY 3 #N/APN2_637 RPGY 4 8409 PN2_638 SAGY 4 6277 PN2_639 PSGY 4 6268 PN2_640 RFGY4 8410 PN2_641 RLGY 4 8411 PN2_642 PYGY 4 6281 PN2_643 GREY 4 8412PN2_644 GRKY 4 8413 PN2_645 GHGW 4 8414 PN2_646 RVGY 4 8415 PN2_647 GLGL4 8416 PN2_648 GHGY 4 8417 PN2_649 RDGY 4 4410 PN2_650 RRVY 4 8418PN2_651 GLGW 4 8419 PN2_652 GHGL 4 8420 PN2_653 GLGY 4 8421 PN2_654 PVGY4 8422 PN2_655 GPGY 4 8423 PN2_656 GPGW 4 8424 PN2_657 PDGY 4 5829PN2_658 GPGL 4 8425 PN2_659 GVTA 4 8426 PN2_660 GRLY 4 8427 PN2_661 RRAY4 8428 PN2_662 GHTQ 4 8429 PN2_663 GVGL 4 8430 PN2_664 SYGY 4 4434PN2_665 RRQY 4 8431 PN2_666 GATQ 4 8432 PN2_667 PFGY 4 8433 PN2_668 GVGY4 8434 PN2_669 GVGW 4 8435 PN2_670 RAGY 4 6278 PN2_671 GATA 4 8436PN2_672 RYGY 4 6262 PN2_673 GRAY 4 8437 PN2_674 GRMY 4 8438 PN2_675 GRTY4 8439 PN2_676 PPGY 4 8440 PN2_677 RRPY 4 8441 PN2_678 RHGY 4 8442PN2_679 GDGY 4 5813 PN2_680 SVGY 4 8443 PN2_681 GVTQ 4 8444 PN2_682 GDGW4 8445 PN2_683 GRQY 4 8446 PN2_684 GDGL 4 8447 PN2_685 SHGY 4 8448PN2_686 GSGY 4 6284 PN2_687 GFGY 4 8449 PN2_688 GFGW 4 8450 PN2_689 GSGW4 8451 PN2_690 GFGL 4 8452 PN2_691 GSGL 4 8453 PN2_692 RRLY 4 8454PN2_693 GFTA 4 8455 PN2_694 PLGY 4 8456 PN2_695 GYTQ 4 8457 PN2_696 GLTQ4 8458 PN2_697 GHTA 4 8459 PN2_698 PHGY 4 8460 PN2_699 GFTQ 4 8461PN2_700 GRVY 4 8462 PN2_701 GYTA 4 8463 PN2_702 GLTA 4 8464 PN2_703 PAGY4 6276 PN2_704 RRKY 4 8465 PN2_705 SSGY 4 4186 PN2_706 GPTQ 4 8466PN2_707 SDGY 4 5805 PN2_708 GPTA 4 8467 PN2_709 GDTQ 4 8468 PN2_710 GAGW4 8469 PN2_711 GAGY 4 6270 PN2_712 GDTA 4 8470 PN2_713 SFGY 4 8471PN2_714 GAGL 4 8472 PN2_715 GSTQ 4 8473 PN2_716 GRPY 4 8474 PN2_717 SLGY4 8475 PN2_718 GSTA 4 8476 PN2_719 GYGL 4 8477 PN2_720 RSGY 4 6269PN2_721 RREY 4 8478 PN2_722 SPGY 4 8479 PN2_723 GYGY 4 6260 PN2_724 RRTY4 8480 PN2_725 GYGW 4 8481 PN2_726 RRMY 4 8482

TABLE 31 Theoretical segment pool of oligonucleotides encoding JHsegments of Example 15. Peptide Degen- SEQ ID Name Degenerate OligoLength erate NO JH4_001 TGGGGACAGGGTACATTGGTCACCGTCTCCTCA 0 8483 JH1_002CATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8484 JH1_003ATTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8485 JH1_004TACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8486 JH1_005CCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8487 JH1_006GTCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8488 JH1_007GATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8489 JH1_008TTCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8490 JH1_009AATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8491 JH1_010AGTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8492 JH1_011ACTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8493 JH1_200GCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 1 8494 JH4_013GATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8495 JH4_016GCTTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8496 JH4_017TTCTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8497 JH4_018GGCTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8498 JH4_019CATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8499 JH4_023AGTTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8500 JH4_024GTTTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8501 JH4_025TACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 8502 JH4_022CSATACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 YES 8503 JH3_012RACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 2 YES 8504 JH5_014RACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 YES 8505 JH3_015RACGTATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 2 YES 8506 JH4_021AMCTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 YES 8507 JH4_020MTATACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 2 YES 8508 JH4_029TTCGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8509 JH4_030ATTGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8510 JH4_031GTGGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8511 JH4_032TTAGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8512 JH4_033TCCGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8513 JH4_034CACGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8514 JH4_035AGAGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8515 JH4_036CCAGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8516 JH4_037AACGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8517 JH4_038ACTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8518 JH4_039GATGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8519 JH4_040GGTGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8520 JH4_041GCAGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8521 JH4_042TACGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8522 JH5_043TTCGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 8523 JH6_044ATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 3 8524 JH1_026TTMCAACACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 YES 8525 JH3_046STAGACGTATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 3 YES 8526 JH3_028TTMGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 3 YES 8527 JH2_027TTMGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 3 YES 8528 JH5_045TYAGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 3 YES 8529 JH3_049GCCTTTGATATTTGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 4 8530 JH4_051TACTTTGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8531 JH4_052AATTTCGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8532 JH4_053GACTTCGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8533 JH4_054CATTTCGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8534 JH4_055TTCTTTGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8535 JH4_056TCTTTTGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8536 JH4_057AGATTCGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8537 JH4_058TTGTTCGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8538 JH4_059CCCTTCGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8539 JH4_060ATTTTCGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8540 JH4_061ACCTTTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8541 JH4_062GGATTCGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8542 JH4_063GTTTTCGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8543 JH4_064GCTTTTGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8544 JH5_065TGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 8545 JH6_068GGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 4 8546 JH6_070YCAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 4 YES 8547 JH6_069KACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 4 YES 8548 JH3_198STATTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 4 YES 8549 JH2_048KACTTCGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 4 YES 8550 JH1_047KACTTCCAACACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 YES 8551 JH5_067GSATTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 YES 8552 JH3_050YCATTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 4 YES 8553 JH5_066AGMTTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 4 YES 8554 JH2_072TGGTACTTCGACTTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 5 8555 JH3_075GACGCATTTGATATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8556 JH3_076TACGCATTTGATATTTGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8557 JH3_077CACGCATTCGACATCTGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8558 JH3_078TTCGCATTCGATATCTGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8559 JH3_079TCAGCTTTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8560 JH3_080AGAGCCTTCGATATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8561 JH3_081TTAGCCTTCGATATCTGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8562 JH3_082GGAGCCTTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 8563 JH4_086GACTATTTTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8564 JH4_087TATTACTTTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8565 JH4_088CACTATTTCGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8566 JH4_089TTCTATTTTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8567 JH4_090AGTTATTTTGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8568 JH4_091AGATACTTTGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8569 JH4_092TTATATTTCGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8570 JH4_093CCCTACTTTGACTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8571 JH4_095GGATATTTCGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8572 JH4_096GTTTACTTTGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8573 JH4_097GCTTACTTTGATTATTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8574 JH4_098AACTACTTCGATTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8575 JH5_099AATTGGTTCGATCCTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8576 JH5_100GATTGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8577 JH5_101TATTGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8578 JH5_102CACTGGTTCGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8579 JH5_103TTCTGGTTTGACCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8580 JH5_104TCTTGGTTTGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8581 JH5_105AGATGGTTTGATCCTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8582 JH5_107GGTTGGTTCGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8583 JH5_109GCTTGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 8584 JH6_110TACGGTATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 5 8585 JH6_112GATGGGATGGATGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 5 8586 JH3_084SCAGCATTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 YES 8587 JH5_106MCATGGTTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 YES 8588 JH2_073RGCTACTTCGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 5 YES 8589 JH4_094AYATACTTCGACTACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 YES 8590 JH5_108RTATGGTTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 YES 8591 JH6_113CWCGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 5 YES 8592 JH6_114KCAGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 5 YES 8593 JH3_083RTAGCATTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 YES 8594 JH1_071RAGTACTTCCAACACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 5 YES 8595 JH3_085AMCGCATTCGACATATGGGGTCAGGGTACAATGGTCACCGTCTCCTCA 5 YES 8596 JH2_074CKATACTTCGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 5 YES 8597 JH6_111KACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 5 YES 8598 JH2_116TACTGGTACTTCGATTTGTGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 6 8599 JH2_117GATTGGTACTTCGATTTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 6 8600 JH5_120GATAATTGGTTCGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8601 JH5_121TATAACTGGTTCGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8602 JH5_122CACAATTGGTTCGACCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8603 JH5_123TTCAATTGGTTTGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8604 JH5_124AGCAACTGGTTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8605 JH5_125AGAAACTGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8606 JH5_126TTAAATTGGTTCGACCCTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8607 JH5_127CCCAATTGGTTTGATCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8608 JH5_128ATAAATTGGTTCGACCCTTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8609 JH5_129ACTAACTGGTTTGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8610 JH5_130GGTAACTGGTTTGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8611 JH5_131GTGAACTGGTTTGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8612 JH5_132GCCAACTGGTTCGATCCCTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8613 JH5_133AACAATTGGTTCGACCCATGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 8614 JH6_134TACTACGGCATGGATGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8615 JH6_136GATTATGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8616 JH6_137TTCTACGGTATGGATGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8617 JH6_138CATTACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8618 JH6_139TTGTACGGAATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8619 JH6_140AACTATGGCATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 8620 JH2_118CWCTGGTACTTCGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 6 YES 8621 JH6_141SCATACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 YES 8622 JH1_115SCAGAATACTTCCAACACTGGGGACAGGGTACATTGGTCACCGTCTCCTCA 6 YES 8623 JH6_135KACTACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 6 YES 8624 JH6_199GKATACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 YES 8625 JH6_142AKCTACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 YES 8626 JH2_119ARCTGGTACTTCGACCTATGGGGGAGAGGTACCTTGGTCACCGTCTCCTCA 6 YES 8627 JH6_143ASATACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 6 YES 8628 JH6_144TATTACTATGGTATGGATGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 7 8629 JH6_145TATTACTATTATATGGATGTTTGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 7 8630 JH6_146GATTACTACGGCATGGATGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 7 8631 JH6_148AACTACTACGGCATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 7 8632 JH6_147CWCTACTACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 7 YES 8633JH6_150 RACTACTACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 7 YES8634 JH6_149 YCATACTACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 7YES 8635 JH6_151TATTACTACTACGGAATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8636 JH6_152AATTATTATTACGGCATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8637 JH6_153GATTACTATTACGGTATGGATGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8638 JH6_154CACTATTACTACGGCATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8639 JH6_155TTCTATTATTATGGTATGGATGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8640 JH6_156TCTTACTACTATGGGATGGACGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8641 JH6_157AGATATTACTACGGCATGGATGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8642 JH6_158TTATACTACTATGGGATGGATGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8643 JH6_159CCTTACTACTATGGCATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8644 JH6_160ACCTATTACTATGGTATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8645 JH6_161GGATACTACTATGGGATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8646 JH6_163GCCTACTATTATGGCATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 8647 JH6_164TACTACTATTATTATATGGACGTTTGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 8 8648 JH6_165AACTACTACTACTATATGGATGTTTGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 8 8649 JH6_166GATTATTATTACTATATGGACGTCTGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 8 8650 JH6_162RTATACTACTACGGAATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 8 YES 8651JH6_168 RGCTACTACTACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 8 YES8652 JH6_167 CMCTACTACTACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 8YES 8653 JH6_169TATTACTATTATTACGGGATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8654JH6_170 AATTATTATTATTATGGGATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98655 JH6_171GACTATTACTATTATGGAATGGATGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8656JH6_172 CATTATTATTATTACGGAATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98657 JH6_173TTCTATTACTATTATGGCATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8658JH6_174 AGCTACTACTATTATGGTATGGACGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98659 JH6_175AGATATTACTACTATGGCATGGATGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8660JH6_176 TTATACTACTATTACGGCATGGATGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98661 JH6_177CCCTATTATTACTACGGAATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8662JH6_178 ATCTATTACTATTATGGCATGGATGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98663 JH6_179ACCTATTACTACTATGGCATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8664JH6_180 GGCTACTATTACTATGGGATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98665 JH6_181GTCTACTATTATTATGGCATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 9 8666JH6_182 GCTTACTATTACTACGGCATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 98667 JH6_183GATTATTATTATTACTATGGTATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8668JH6_184 TACTATTACTACTATTATGGCATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8669 JH6_185CACTACTACTATTATTATGGGATGGACGTATGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8670JH6_186 TTCTATTATTATTATTACGGAATGGACGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8671 JH6_187AGCTACTATTACTACTATGGGATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8672JH6_188 AGATATTACTATTACTATGGTATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8673 JH6_189TTGTACTATTACTATTATGGAATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8674JH6_190 CCTTACTATTATTATTATGGGATGGATGTCTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8675 JH6_191ATATATTACTATTACTACGGGATGGATGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8676JH6_192 ACCTACTATTATTATTACGGGATGGACGTTTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8677 JH6_193GGTTACTATTATTACTACGGGATGGACGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8678JH6_194 GTGTATTACTATTACTACGGGATGGACGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8679 JH6_195GCCTATTACTACTACTATGGGATGGATGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA 10 8680JH6_196 AATTATTATTACTATTACGGTATGGACGTGTGGGGCCAGGGAACAACTGTCACCGTCTCCTCA10 8681 JH6_197KACTACTACTACTACTACTACATGGACGTATGGGGCAAGGGTACAACTGTCACCGTCTCCTCA 10 YES8682

TABLE 32 Theoretical segment pool of unique H3-JH polypeptide segmentsencoded by the oligonucleotides of Table 31. Name Sequence Length SEQ IDNO PJH4_001 0 n/a PJH1_002 H 1 n/a PJH1_003 I 1 n/a PJH1_004 Y 1 n/aPJH1_005 P 1 n/a PJH1_006 V 1 n/a PJH1_007 D 1 n/a PJH1_008 F 1 n/aPJH1_009 N 1 n/a PJH1_010 S 1 n/a PJH1_011 T 1 n/a PJH1_200 A 1 n/aPJH4_013 DY 2 n/a PJH4_016 AY 2 n/a PJH4_017 FY 2 n/a PJH4_018 GY 2 n/aPJH4_019 HY 2 n/a PJH4_023 SY 2 n/a PJH4_024 VY 2 n/a PJH4_025 YY 2 n/aPJH3_012A NI 2 n/a PJH3_012B DI 2 n/a PJH3_015A NV 2 n/a PJH3_015B DV 2n/a PJH4_020A LY 2 n/a PJH4_020B IY 2 n/a PJH4_021A NY 2 n/a PJH4_021BTY 2 n/a PJH4_022A PY 2 n/a PJH4_022B RY 2 n/a PJH5_014A NP 2 n/aPJH5_014B DP 2 n/a PJH4_029 FDY 3 n/a PJH4_030 IDY 3 n/a PJH4_031 VDY 3n/a PJH4_032 LDY 3 n/a PJH4_033 SDY 3 n/a PJH4_034 HDY 3 n/a PJH4_035RDY 3 n/a PJH4_036 PDY 3 n/a PJH4_037 NDY 3 n/a PJH4_038 TDY 3 n/aPJH4_039 DDY 3 n/a PJH4_040 GDY 3 n/a PJH4_041 ADY 3 n/a PJH4_042 YDY 3n/a PJH5_043 FDP 3 n/a PJH6_044 MDV 3 n/a PJH1_026A LQH 3 n/a PJH1_026BFQH 3 n/a PJH2_027A FDL 3 n/a PJH2_027B LDL 3 n/a PJH3_028A FDI 3 n/aPJH3_028B LDI 3 n/a PJH3_046A VDV 3 n/a PJH3_046B LDV 3 n/a PJH5_045ALDP 3 n/a PJH5_045B SDP 3 n/a PJH3_049 AFDI 4 4539 PJH4_051 YFDY 4 4567PJH4_052 NFDY 4 4580 PJH4_053 DFDY 4 4581 PJH4_054 HFDY 4 4582 PJH4_055FFDY 4 4583 PJH4_056 SFDY 4 4584 PJH4_057 RFDY 4 4585 PJH4_058 LFDY 44586 PJH4_059 PFDY 4 4587 PJH4_060 IFDY 4 4588 PJH4_061 TFDY 4 4589PJH4_062 GFDY 4 4590 PJH4_063 VFDY 4 4591 PJH4_064 AFDY 4 4592 PJH5_065WFDP 4 4596 PJH6_068 GMDV 4 4641 PJH1_047A YFQH 4 4489 PJH1_047B DFQH 44514 PJH2_048A DFDL 4 4537 PJH2_048B YFDL 4 4529 PJH3_050A PFDI 4 4554PJH3_050B SFDI 4 4553 PJH3_198A VFDI 4 4563 PJH3_198B LFDI 4 4558PJH5_066A RFDP 4 4622 PJH5_066B SFDP 4 4625 PJH5_067A GFDP 4 4623PJH5_067B AFDP 4 4633 PJH6_069A YMDV 4 4687 PJH6_069B DMDV 4 8683PJH6_070A PMDV 4 8684 PJH6_070B SMDV 4 8685 PJH2_072 WYFDL 5 4528PJH3_075 DAFDI 5 4538 PJH3_076 YAFDI 5 4540 PJH3_077 HAFDI 5 4541PJH3_078 FAFDI 5 4542 PJH3_079 SAFDI 5 4543 PJH3_080 RAFDI 5 4544PJH3_081 LAFDI 5 4545 PJH3_082 GAFDI 5 4549 PJH4_086 DYFDY 5 4566PJH4_087 YYFDY 5 4568 PJH4_088 HYFDY 5 4569 PJH4_089 FYFDY 5 4570PJH4_090 SYFDY 5 4571 PJH4_091 RYFDY 5 4572 PJH4_092 LYFDY 5 4573PJH4_093 PYFDY 5 4574 PJH4_095 GYFDY 5 4577 PJH4_096 VYFDY 5 4578PJH4_097 AYFDY 5 4579 PJH4_098 NYFDY 5 4593 PJH5_099 NWFDP 5 4595PJH5_100 DWFDP 5 4609 PJH5_101 YWFDP 5 4610 PJH5_102 HWFDP 5 4611PJH5_103 FWFDP 5 4612 PJH5_104 SWFDP 5 4613 PJH5_105 RWFDP 5 4614PJH5_107 GWFDP 5 4619 PJH5_109 AWFDP 5 4621 PJH6_110 YGMDV 5 4640PJH6_112 DGMDV 5 8686 PJH1_071A EYFQH 5 4488 PJH1_071B KYFQH 5 4502PJH2_073A SYFDL 5 8687 PJH2_073B GYFDL 5 4533 PJH2_074A RYFDL 5 4534PJH2_074B LYFDL 5 8688 PJH3_083A IAFDI 5 4547 PJH3_083B VAFDI 5 4550PJH3_084A PAFDI 5 4546 PJH3_084B AAFDI 5 4551 PJH3_085A NAFDI 5 4565PJH3_085B TAFDI 5 4548 PJH4_094A IYFDY 5 4575 PJH4_094B TYFDY 5 4576PJH5_106A PWFDP 5 4616 PJH5_106B TWFDP 5 4618 PJH5_108A IWFDP 5 4617PJH5_108B VWFDP 5 4620 PJH6_111A YYMDV 5 4686 PJH6_111B DYMDV 5 8689PJH6_113A HGMDV 5 8690 PJH6_113B LGMDV 5 8691 PJH6_114A SGMDV 5 8692PJH6_114B AGMDV 5 8693 PJH2_116 YWYFDL 6 4527 PJH2_117 DWYFDL 6 4530PJH5_120 DNWFDP 6 4594 PJH5_121 YNWFDP 6 4597 PJH5_122 HNWFDP 6 4598PJH5_123 FNWFDP 6 4599 PJH5_124 SNWFDP 6 4600 PJH5_125 RNWFDP 6 4601PJH5_126 LNWFDP 6 4602 PJH5_127 PNWFDP 6 4603 PJH5_128 INWFDP 6 4604PJH5_129 TNWFDP 6 4605 PJH5_130 GNWFDP 6 4606 PJH5_131 VNWFDP 6 4607PJH5_132 ANWFDP 6 4608 PJH5_133 NNWFDP 6 4634 PJH6_134 YYGMDV 6 4639PJH6_136 DYGMDV 6 8694 PJH6_137 FYGMDV 6 8695 PJH6_138 HYGMDV 6 8696PJH6_139 LYGMDV 6 8697 PJH6_140 NYGMDV 6 8698 PJH1_115A AEYFQH 6 4526PJH1_115B PEYFQH 6 4491 PJH2_118A LWYFDL 6 8699 PJH2_118B HWYFDL 6 4531PJH2_119A NWYFDL 6 4532 PJH2_119B SWYFDL 6 8700 PJH6_135A DYYMDV 6 8701PJH6_135B YYYMDV 6 4685 PJH6_141A AYGMDV 6 8702 PJH6_141B PYGMDV 6 8703PJH6_142A SYGMDV 6 8704 PJH6_142B IYGMDV 6 8705 PJH6_143A TYGMDV 6 8706PJH6_143B RYGMDV 6 8707 PJH6_199A GYGMDV 6 8708 PJH6_199B VYGMDV 6 8709PJH6_144 YYYGMDV 7 4638 PJH6_145 YYYYMDV 7 4684 PJH6_146 DYYGMDV 7 8710PJH6_148 NYYGMDV 7 8711 PJH6_147A LYYGMDV 7 8712 PJH6_147B HYYGMDV 78713 PJH6_149A SYYGMDV 7 8714 PJH6_149B PYYGMDV 7 8715 PJH6_150A NYYYMDV7 8716 PJH6_150B DYYYMDV 7 8717 PJH6_151 YYYYGMDV 8 4637 PJH6_152NYYYGMDV 8 4667 PJH6_153 DYYYGMDV 8 4668 PJH6_154 HYYYGMDV 8 4669PJH6_155 FYYYGMDV 8 4670 PJH6_156 SYYYGMDV 8 4671 PJH6_157 RYYYGMDV 84672 PJH6_158 LYYYGMDV 8 4673 PJH6_159 PYYYGMDV 8 4674 PJH6_160 TYYYGMDV8 4676 PJH6_161 GYYYGMDV 8 4677 PJH6_163 AYYYGMDV 8 4679 PJH6_164YYYYYMDV 8 4683 PJH6_165 NYYYYMDV 8 4713 PJH6_166 DYYYYMDV 8 4714PJH6_162A VYYYGMDV 8 4678 PJH6_162B IYYYGMDV 8 4675 PJH6_167A HYYYYMDV 84715 PJH6_167B PYYYYMDV 8 4720 PJH6_168A SYYYYMDV 8 4717 PJH6_168BGYYYYMDV 8 4723 PJH6_169 YYYYYGMDV 9 4636 PJH6_170 NYYYYGMDV 9 4654PJH6_171 DYYYYGMDV 9 4655 PJH6_172 HYYYYGMDV 9 4656 PJH6_173 FYYYYGMDV 94657 PJH6_174 SYYYYGMDV 9 4658 PJH6_175 RYYYYGMDV 9 4659 PJH6_176LYYYYGMDV 9 4660 PJH6_177 PYYYYGMDV 9 4661 PJH6_178 IYYYYGMDV 9 4662PJH6_179 TYYYYGMDV 9 4663 PJH6_180 GYYYYGMDV 9 4664 PJH6_181 VYYYYGMDV 94665 PJH6_182 AYYYYGMDV 9 4666 PJH6_183 DYYYYYGMDV 10 4635 PJH6_184YYYYYYGMDV 10 4642 PJH6_185 HYYYYYGMDV 10 4643 PJH6_186 FYYYYYGMDV 104644 PJH6_187 SYYYYYGMDV 10 4645 PJH6_188 RYYYYYGMDV 10 4646 PJH6_189LYYYYYGMDV 10 4647 PJH6_190 PYYYYYGMDV 10 4648 PJH6_191 IYYYYYGMDV 104649 PJH6_192 TYYYYYGMDV 10 4650 PJH6_193 GYYYYYGMDV 10 4651 PJH6_194VYYYYYGMDV 10 4652 PJH6_195 AYYYYYGMDV 10 4653 PJH6_196 NYYYYYGMDV 104680 PJH6_197A DYYYYYYMDV 10 4681 PJH6_197B YYYYYYYMDV 10 4688

EQUIVALENTS

Those skilled in the art will recognize, or be able to ascertain usingno more than routine experimentation, many equivalents to the specificembodiments and methods described herein. Such equivalents are intendedto be encompassed by the scope of the following claims.

APPENDIX A GI NUMBERS OF 3,571 SEQUENCES IN THE HEALTHY PREIMMUNE SET(HPS) 33628 1052674 1685242 1770847 37745 1052676 1685246 1770848 377471052683 1685248 1770851 37749 1052685 1685250 1770852 37751 10526911685252 1770853 37753 1052692 1685254 1770854 37755 1052693 16852561770855 37757 1052695 1685258 1770860 37759 1154682 1685260 177086137761 1154691 1685264 1770865 37763 1154698 1685266 1770866 377651154699 1685268 1770867 37767 1154706 1770744 1770869 37769 11547101770746 1770870 37773 1154713 1770747 1770872 37777 1154715 17707511770874 38383 1154724 1770755 1770875 38391 1154754 1770756 177087638393 1154769 1770758 1770877 38397 1154770 1770759 1770878 384011154805 1770761 1770879 185292 1154807 1770763 1770880 264183 11548081770765 1770881 297147 1154809 1770766 1770882 306949 1154810 17707701770883 306951 1154811 1770771 1770884 306953 1154813 1770772 1770885483332 1154818 1770775 1770887 483333 1154820 1770776 1770888 4833351154822 1770777 1770891 483336 1154824 1770779 1770892 483338 11548251770780 1770893 483339 1154834 1770783 1770894 483348 1154837 17707841770895 483350 1154838 1770785 1770896 510999 1154839 1770789 1770898547164 1154840 1770791 1770902 587252 1154841 1770792 1770904 5872541154843 1770793 1770905 587266 1154844 1770794 1770906 587276 11548451770795 1770908 587278 1154847 1770796 1770909 587280 1154848 17707971770910 587286 1197299 1770799 1770911 587288 1197300 1770800 1770912587291 1197304 1770801 1770913 587293 1197307 1770805 1770914 5872951197308 1770806 1770915 587299 1197309 1770807 1770916 587301 11973121770808 1770918 587304 1197313 1770809 1770922 587306 1197314 17708101770932 587308 1197315 1770811 1770936 587311 1197316 1770812 1770937587313 1197318 1770813 1770950 587315 1197319 1770814 1770952 5873171197321 1770815 1770954 1052611 1197322 1770816 1770958 1052620 11973231770817 1770961 1052622 1197324 1770818 1770962 1052626 1197325 17708201770963 1052627 1197326 1770822 1770964 1052634 1197327 1770824 17709671052637 1197328 1770826 1770969 1052639 1495508 1770829 1770971 10526401495511 1770830 1770972 1052642 1495512 1770831 1770974 1052644 14955161770833 1770976 1052655 1495518 1770835 1770979 1052656 1592729 17708361770981 1052657 1685210 1770837 1770982 1052658 1685220 1770839 17709831052659 1685222 1770840 1770989 1052662 1685228 1770843 1770992 10526681685234 1770844 1770994 1052669 1685238 1770845 1770995 1052671 16852401770846 1770997 1770998 1791142 3170752 3170974 1771002 1791144 31707543170978 1771004 1791152 3170756 3170980 1771008 1791154 3170758 31709841771010 1791160 3170760 3170986 1771014 1791164 3170762 3170988 17710161791176 3170764 3170990 1771017 1791182 3170766 3170992 1771018 17911843170768 3171006 1771022 1791186 3170772 3171008 1771026 1791190 31707743171010 1771027 1791194 3170778 3171016 1771029 1791196 3170782 31710181771033 1791200 3170784 3171020 1771034 1791204 3170786 3171022 17710351791206 3170788 3171024 1771036 1869905 3170794 3171026 1771038 18699073170796 3171028 1771039 1869912 3170802 3171030 1771042 1869913 31708083171038 1771044 1869915 3170810 3171040 1771045 1869918 3170812 31710421771055 1869919 3170816 3171044 1771057 1934921 3170820 3171242 17710582367538 3170822 3608440 1771059 2388836 3170824 3608462 1771060 23888373170826 3954953 1771061 2388839 3170830 3954955 1771063 2388840 31708324530538 1791008 2388841 3170834 4530544 1791010 2388842 3170836 47537411791012 2388843 3170840 4959477 1791018 2388846 3170842 4995315 17910202388847 3170844 4995317 1791026 2388848 3170846 4995319 1791028 23888513170848 4995321 1791030 2388852 3170852 4995323 1791032 2388853 31708544995325 1791034 2388856 3170856 4995327 1791036 2388859 3170858 49953291791040 2388861 3170862 4995331 1791042 2388862 3170864 4995333 17910462388863 3170866 4995335 1791050 2388864 3170868 4995337 1791052 23888653170870 4995339 1791054 2388868 3170872 4995341 1791058 2388871 31708744995343 1791060 2388873 3170876 4995345 1791062 2388875 3170878 49953471791064 2388876 3170880 4995349 1791072 2388878 3170882 4995351 17910742773082 3170884 4995353 1791076 3170658 3170890 4995355 1791078 31706623170894 4995357 1791080 3170664 3170898 4995359 1791082 3170668 31709024995361 1791084 3170670 3170908 4995365 1791086 3170686 3170910 49953671791088 3170688 3170916 4995375 1791090 3170692 3170918 4995383 17910963170694 3170922 4995385 1791098 3170696 3170924 4995389 1791100 31707023170926 4995391 1791104 3170704 3170930 4995393 1791106 3170712 31709324995397 1791108 3170714 3170934 4995399 1791110 3170716 3170936 49954001791112 3170720 3170938 4995404 1791114 3170722 3170944 4995406 17911163170726 3170946 4995408 1791118 3170728 3170954 4995410 1791122 31707303170958 4995418 1791124 3170734 3170960 4995422 1791130 3170736 31709644995426 1791132 3170738 3170966 4995428 1791134 3170740 3170968 49954301791136 3170748 3170970 4995432 4995434 5834089 6531600 8489286 49954365834091 6723523 8489289 4995438 5834093 6723525 8489291 4995440 58340956723527 11137164 4995442 5834097 6723531 11137170 4995446 58340996723535 11137172 4995456 5834101 6723537 11137174 4995462 58341036723543 11137178 4995466 5834105 6723545 11137183 4995470 58341076723549 11137186 4995474 5834109 6723551 11137188 4995476 58341136723558 11137196 4995478 5834115 6723565 11137200 4995480 58341196723581 11137205 4995482 5834121 6723583 11137215 4995484 58341236723595 11137219 4995486 5834125 6723597 11137229 4995488 58341276723599 11137231 4995490 5834129 7161042 11137242 4995492 58341317161061 11137251 4995494 5834133 7161129 11137253 4995496 58341357161136 11137261 4995498 5834137 7161164 11137262 4995500 58341398249510 11137274 4995502 5834141 8249514 11137276 4995504 58341438249518 11137279 4995506 5834145 8249524 11137281 4995508 58341478249528 11137283 4995510 5834149 8249538 11137285 4995512 58341518249546 11137289 4995514 5834153 8249552 11137290 4995516 58341558249554 11137293 4995520 5834159 8249558 11137295 4995524 58341618249560 11137298 4995530 5834163 8249562 11137301 4995535 58341658249566 11137303 4995537 5834169 8249568 11137305 4995539 58341758249608 11137307 4995549 5834177 8249622 11137309 4995555 58341798249632 11137313 4995557 5834183 8249650 11137315 4995563 58341858249652 11137317 4995569 5834187 8249654 11137319 4995575 58341918249656 11137322 4995581 5834193 8249662 11137326 4995589 58341958249674 11137329 4995591 5834197 8249682 11137333 5833973 58341998249698 11137335 5833980 5834201 8249712 11137339 5833984 58342038249716 11137343 5833986 5834205 8249718 11137348 5834003 58342078249730 11137350 5834011 5834209 8249738 11137352 5834015 58342138249740 11137354 5834019 5834215 8249744 11137359 5834031 60130398249754 11137361 5834035 6013043 8249756 11137363 5834037 60130458249760 11137365 5834039 6531445 8249772 11137367 5834041 65314578249778 11137369 5834043 6531461 8249784 11137371 5834047 65314658249786 11137373 5834049 6531481 8249788 11137375 5834051 65314898249790 11137377 5834053 6531493 8249812 11137379 5834055 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162950314162950431 159035596 162950139 162950315 162950432 159035597 162950140162950316 162950433 159035598 162950141 162950317 162950434 159035602162950143 162950318 162950435 159035603 162950146 162950319 162950437159035604 162950147 162950321 162950438 162950439 162950566 162950674162950440 162950568 162950675 162950441 162950570 162950676 162950442162950571 162950685 162950444 162950572 162950686 162950445 162950573162950688 162950446 162950574 162950689 162950447 162950575 162950691162950449 162950577 162950692 162950450 162950578 162950693 162950451162950579 162950694 162950453 162950580 162950695 162950454 162950581162950696 162950456 162950582 162950697 162950461 162950583 162950698162950463 162950584 162950710 162950464 162950585 162950714 162950465162950586 162950716 162950466 162950587 162950720 162950467 162950589162950724 162950469 162950590 162950725 162950470 162950591 162950726162950471 162950592 162950728 162950474 162950593 162950729 162950476162950594 194719560 162950481 162950596 194719575 162950483 162950597218454113 162950484 162950598 218454117 162950485 162950599 219937557162950487 162950600 162950489 162950602 162950490 162950604 162950491162950605 162950492 162950606 162950493 162950609 162950494 162950610162950496 162950611 162950498 162950613 162950500 162950614 162950503162950615 162950504 162950617 162950514 162950618 162950515 162950619162950516 162950620 162950517 162950621 162950518 162950622 162950519162950626 162950520 162950627 162950522 162950628 162950525 162950629162950526 162950631 162950527 162950632 162950528 162950633 162950529162950634 162950530 162950635 162950531 162950640 162950532 162950641162950534 162950642 162950535 162950644 162950536 162950645 162950537162950646 162950539 162950647 162950540 162950649 162950542 162950650162950543 162950651 162950546 162950652 162950547 162950654 162950551162950655 162950552 162950656 162950555 162950659 162950556 162950660162950557 162950661 162950558 162950662 162950559 162950665 162950562162950666 162950564 162950671 162950565 162950673

What is claimed is:
 1. A library comprising synthetic polynucleotidesthat encode an antibody heavy chain or alternative scaffold containingat least about 10⁴ unique antibody CDRH3 amino acid sequences, whereineach of the polynucleotides encoding the at least about 10⁴ uniqueantibody CDRH3 amino acid sequences has an antibody CDRH3 amino acidsequence represented by the following formula: [TN1]-[DH]-[N2]- [H3-JH],wherein: TN1 is a polypeptide corresponding to any of the TN1polypeptides of Tables 10 and 18-26, or a polypeptide produced bytranslation of any of the TN1 polynucleotides of Tables 25-26; DH is apolypeptide corresponding to any of the DH polypeptides of Tables 11,17-25 and 28, or a polypeptide produced by translation of any of theDH-encoding polynucleotides of Tables 16, 25 and 27; N2 is a polypeptidecorresponding to any of the N2 polypeptides of Tables 12, 18-25 and 30,or a polypeptide produced by translation of any of the N2-encodingpolynucleotides of Tables 25 and 29; H3-JH is a polypeptidecorresponding to any of the H3-JH polypeptides of Tables 13, 15, 18-25and 32, or a polypeptide produced by translation of any of theH3-JH-encoding polynucleotides of Tables 14, 25 and 31; wherein thediversity of the polynucleotides encoding the at least about 10⁴ uniqueantibody CDRH3 amino acid sequences is created by each of thepolynucleotides having a CDRH3 sequence that is different from the CDRH3sequence of every other polynucleotide; wherein the antibody heavy chainis a variable domain with framework (FRM) and complementary determiningregions (CDR) comprising FRMH1-CDRH1-FRMH2-CDRH2-FRMH3-CDRH3-FRMH4; andwherein the alternative scaffold is selected from the group consistingof fibronectin, the β-sandwich, lipocalin, EETI-IFAGRP,BPTIFLAC1-D1/IT1-D2, thioredoxin, protein A, ankyrin repeats,γB-crystallin/ubiquitin, CTLD₃ and (LDLR-A module)₃.
 2. The library ofclaim 1, wherein the polynucleotides encode CDRH3 polypeptides producedby the sets of TN1, DH, N2, and H3-JH polypeptides provided in any oneof Tables 23-25.
 3. The library of claim 1, wherein the polynucleotidesencode CDRI-13 polypeptides produced by the set of TN1 polypeptidesprovided in Table 26, the set of DH polypeptides provided in Table 28,the set of N2 polypeptides provided in Table 30 and the set of H3-JHpolypeptides provided in Table
 32. 4. The library of claim 1 whereinnumber of N-linked glycosylation sites, deamidation motifs, and/or Cysresidues are reduced or eliminated in comparison to libraries producedby amplification of a repertoire from a biological source.
 5. Thelibrary of claim 1, further comprising a polynucleotide encoding one ormore light chain variable domain polypeptides.
 6. The library of claim5, wherein the polypeptides are expressed as full-length IgGs.
 7. Thepolypeptide expression products of the library of claim
 6. 8. Anantibody isolated from the polypeptide expression products of claim 7.9. A vector containing the library of claim
 1. 10. A host cellcontaining the vector of claim
 9. 11. The host cell of claim 10, whereinthe host cell is a yeast cell.
 12. The yeast cell of claim 11, whereinthe yeast is Saccharomyces cerevisiae.
 13. A kit containing the libraryof claim 1.